MALDI-TOF analysis of steroid/PAH-modified DNA adducts at the femtomole level
- Washington Univ., St. Louis, MO (United States)
- Univ. of Nebraska Medical Center, Omaha, NE (United States); and others
Covalent binding of polycyclic aromatic hydrocarbons (PAH`s) and steroids to DNA to form adducts is one of the first events in the process of tumor initiation in carcinogenesis. Structure elucidation and characterization of these adducts provide important information that leads to further understanding of their biological metabolic pathways. In in vivo and in vitro steroid/PAH-DNA binding studies, the reaction products (adducts) are often of low amount (low picomole to femtomole). Previous results from this laboratory have shown that the sensitivity of MALDI-TOF can be improved by proper matrix selection. An increase in sensitivity can also be obtained with the use of d-fucose as a co-matrix. In this study 4-phenyl-{alpha}-cyanocinnamic acid, PCC, 4-benzyloxy-{alpha}-cyanocinnamic acid, BCC, ferulic acid, FA, {alpha}-cyano-4-hydroxycinnamic acid, 4HCCA, and 3-(2-naphthyl)-2-cyanoacrylic NCA, were used in the determination of the limit of detection for two different DNA adducts dibenzocarbazole-5-N7Ade, and 4-hydroxyestrone-N7Gua.
- OSTI ID:
- 210632
- Report Number(s):
- CONF-9505261-; TRN: 96:001157-0071
- Resource Relation:
- Conference: 43. American Society of Mass Spectrometry (ASMS) conference on mass spectrometry and allied topics, Atlanta, GA (United States), 21-26 May 1995; Other Information: PBD: 1995; Related Information: Is Part Of Proceedings of the 43rd ASMS conference on mass spectrometry and allied topics; PB: 1411 p.
- Country of Publication:
- United States
- Language:
- English
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