Measurement of interaction force between nanoarrayed integrin {alpha}{sub v}{beta}{sub 3} and immobilized vitronectin on the cantilever tip
- Chungbuk BIT Research-Oriented University Consortium, Chungbuk National University, Cheongju 361-763 (Korea, Republic of)
- Department of Semiconductor Engineering, Research Institute of UbiquitousBio-Information Technology, Chungbuk National University, Cheongju 361-763 (Korea, Republic of)
- Department of Biochemistry, Protein Chip Center, Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763 (Korea, Republic of)
- Department of Biological Science, Hoseo University, Ansan 336-795 (Korea, Republic of)
Protein nanoarrays containing integrin {alpha}{sub v}{beta}{sub 3} or BSA were fabricated on ProLinker{sup TM}-coated Au surface by dip-pen nanolithography (DPN). An atomic force microscope (AFM) tip coated with ProLinker{sup TM} was modified by vitronectin. We measured the interaction force between nanoarrayed integrin {alpha}{sub v}{beta}{sub 3} or BSA and immobilized vitronectin on the cantilever tip by employing tethering-unbinding method. The unbinding force between integrin {alpha}{sub v}{beta}{sub 3} and vitronectin (1087 {+-} 62 pN) was much higher than that of between BSA and vitronectin (643 {+-} 74 pN). These results demonstrate that one can distinguish a specific protein interaction from non-specific interactions by means of force measurement on the molecular interactions between the nanoarrayed protein and its interacting protein on the AFM tip.
- OSTI ID:
- 21032951
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 362, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2007.08.084; PII: S0006-291X(07)01798-6; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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