Immune responses to an encapsulated allogeneic islet {beta}-cell line in diabetic NOD mice

Journal Article · Fri Feb 03 00:00:00 EST 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.11.180· OSTI ID:20798785

Black, Sasha P ^[1]; Constantinidis, Ioannis ^[2]; Cui, Hong ^[3]; Tucker-Burden, Carol ^[3]; Weber, Collin J ^[3]; Safley, Susan A ^[3]

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1. Charles River Laboratories, Pre-clinical Services Montreal, Senneville, Que., H9X 3R3 (Canada)
2. Department of Medicine, Division of Endocrinology, University of Florida, Gainesville, FL 32610 (United States)
3. Department of Surgery, Emory University School of Medicine, Atlanta, GA 30322 (United States)

Our goal is to develop effective islet grafts for treating type 1 diabetes. Since human islets are scarce, we evaluated the efficacy of a microencapsulated insulin-secreting conditionally transformed allogeneic {beta}-cell line ({beta}TC-tet) in non-obese diabetic mice treated with tetracycline to inhibit cell growth. Relatively low serum levels of tetracycline controlled proliferation of {beta}TC-tet cells without inhibiting effective control of hyperglycemia in recipients. There was no significant host cellular reaction to the allografts or host cell adherence to microcapsules, and host cytokine levels were similar to those of sham-operated controls. We conclude that encapsulated allogeneic {beta}-cell lines may be clinically relevant, because they effectively restore euglycemia and do not elicit a strong cellular immune response following transplantation. To our knowledge, this is First extensive characterization of the kinetics of host cellular and cytokine responses to an encapsulated islet cell line in an animal model of type 1 diabetes.

• OSTI ID: 20798785
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 340, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2005.11.180; PII: S0006-291X(05)02689-6; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ALGINATES
ANIMAL CELLS
CELL PROLIFERATION
GRAFTS
INSULIN
LYMPHOKINES
MICE
TETRACYCLINES