Signalling mechanisms of SDF-induced endothelial cell proliferation and migration
- Justus-Liebig-University of Giessen, Department of Cardiology and Angiology (Germany)
The aim of our study was to investigate the effect of stromal-derived factor-1-{alpha} (SDF-1-{alpha}) on endothelial angiogenic effects. SDF-1-{alpha} (50 ng/ml) increased the number of cultured endothelial cells from 33,653 {+-} 1183 to 55,398 {+-} 2741, which significantly reduced by adding the BK{sub Ca}-inhibitor iberiotoxin, or the endothelial nitric oxide synthase-blocker, L-NMMA (n = 24, p < 0.05). Using the 'Fences'-assay a significant increase of HUVEC migration induced by SDF-1-{alpha} was reported, which was blocked by the addition of iberiotoxin or L-NMMA (n = 12, p < 0.05). BK{sub Ca} open-state probability (NPo) was analysed using the patch-clamp technique and NPo was increased from 0.003 (control) to 0.052 (SDF-1-{alpha}; n = 10, p < 0.05). NO synthesis was measured using a cGMP-radioimmunoassay. A significant increase of cGMP levels from 0.952 pmol/mg protein to 2.179 pmol/mg protein was observed, that was abolished by L-NMMA and significantly reduced by iberiotoxin (n = 15, p < 0.05). SDF-1-{alpha} increases endothelial proliferation and migration involving the activation of BK{sub Ca} and an increased production of NO.
- OSTI ID:
- 20711010
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 335, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2005.08.006; PII: S0006-291X(05)01674-8; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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