Cell cycle regulation of human immunodeficiency virus type 1 integration in T cells: antagonistic effects of nuclear envelope breakdown and chromatin condensation
- EMI-0013 Institut National de la Sante et de la Recherche Medicale-Universite Paris 7, 75010 Paris (France) and Laboratoire d'Immunologie Cellulaire et Immunopathologie de l'Ecole Pratique des Hautes Etudes, Institut Universitaire d'Hematologie, hopital Saint-Louis, 75010 Paris (France) and Laboratoire de Biochimie and JFR 3012 Associee a l'Agence Universitaire Francophone (AUPELF-UREF), Faculte des Sciences, Rabat (Morocco)
- EMI-0013 Institut National de la Sante et de la Recherche Medicale-Universite Paris 7, 75010 Paris (France) and Laboratoire d'Immunologie Cellulaire et Immunopathologie de l'Ecole Pratique des Hautes Etudes, Institut Universitaire d'Hematologie, hopital Saint-Louis, 75010 Paris (France)
- EMI-0013 Institut National de la Sante et de la Recherche Medicale-Universite Paris 7, 75010 Paris (France) and Laboratoire d'Immunologie Cellulaire et Immunopathologie de l'Ecole Pratique des Hautes Etudes, Institut Universitaire d'Hematologie, hopital Saint-Louis, 75010 Paris (France); and others
We examined the influence of mitosis on the kinetics of human immunodeficiency virus type 1 integration in T cells. Single-round infection of cells arrested in G1b or allowed to synchronously proceed through division showed that mitosis delays virus integration until 18-24 h postinfection, whereas integration reaches maximum levels by 15 h in G1b-arrested cells. Subcellular fractionation of metaphase-arrested cells indicated that, while nuclear envelope disassembly facilitates docking of viral DNA to chromatin, chromosome condensation directly antagonizes and therefore delays integration. As a result of the balance between the two effects, virus integration efficiency is eventually up to threefold greater in dividing cells. At the single-cell level, using a green fluorescent protein-expressing reporter virus, we found that passage through mitosis leads to prominent asymmetric segregation of the viral genome in daughter cells without interfering with provirus expression.
- OSTI ID:
- 20637175
- Journal Information:
- Virology, Vol. 329, Issue 1; Other Information: DOI: 10.1016/j.virol.2004.08.022; PII: S0042-6822(04)00515-X; Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
Similar Records
Expression of the human immunodeficiency virus envelope glycoprotein is restricted to basolateral surfaces of polarized epithelial cells
Vaccination of rhesus macaques with a vif-deleted simian immunodeficiency virus proviral DNA vaccine