Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Expression of the human immunodeficiency virus envelope glycoprotein is restricted to basolateral surfaces of polarized epithelial cells

Journal Article · · J. Virol.; (United States)
OSTI ID:5822139
;
Polarized epithelial cells exhibit apical (lumenal) and basolateral (serosal) membrane domains that are separated by circumferential tight junctions. In such cells, enveloped viruses that mature by budding at cell surfaces are released at particular membrane domains. The authors have used a vaccinia virus recombinant to investigate the site of surface expression of the human immunodeficiency virus type 1 envelope glycoprotein in Madin-Darby canine kidney cells. Cells were infected with the vaccinia virus recombinant, and surface expression of the glycoprotein was analyzed by indirect immunofluorescence, /sup 125/I-protein A binding, and immunoelectron microscopy. The glycoprotein appeared exclusively at the basolateral surface as early as 2 h postinfection and reached a maximum level at 8 h postinfection. The gp120 glycoprotein was found to be secreted efficiently into culture medium, and this secretion occurred exclusively at the basolateral surface.
Research Organization:
Univ. of Alabama, Birmingham (USA)
OSTI ID:
5822139
Journal Information:
J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 63:2; ISSN JOVIA
Country of Publication:
United States
Language:
English

Similar Records

Humoral immune response to the entire human immunodeficiency virus envelope glycoprotein made in insect cells
Journal Article · Thu Oct 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:7242205
Steady-state distribution and biogenesis of endogenous Madin-Darby canine kidney glycoproteins: Evidence for intracellular sorting and polarized cell surface delivery
Journal Article · Tue Oct 31 23:00:00 EST 1989 · Journal of Cell Biology; (USA) · OSTI ID:5102139
Galactosyl ceramide or a derivative is an essential component of the neural receptor for human immunodeficiency virus type 1 envelope glycoprotein gp120
Journal Article · Thu Aug 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States) · OSTI ID:5822654

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AIDS VIRUS
AMINO ACIDS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
ELECTROPHORESIS
EPITHELIUM
GLUCOPROTEINS
GLYCOPROTEINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LEUCINE
MEMBRANE TRANSPORT
MEMBRANES
MICROORGANISMS
MICROSCOPY
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PATHOGENESIS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RNA
SACCHARIDES
SECRETION
TISSUES
TRITIUM COMPOUNDS
VACCINIA VIRUS
VIRUSES