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Title: Kinetic Effects on Self-Assembly and Function of Protein-Polymer Bioconjugates in Thin Films Prepared by Flow Coating

Abstract

The self-assembly of nanostructured globular protein arrays in thin films is demonstrated using protein–polymer block copolymers based on a model protein mCherry and the polymer poly(oligoethylene glycol acrylate) (POEGA). Conjugates are flow coated into thin films on a poly(ethylene oxide) grafted Si surface, forming self-assembled cylindrical nanostructures with POEGA domains selectively segregating to the air–film interface. Long-range order and preferential arrangement of parallel cylinders templated by selective surfaces are demonstrated by controlling relative humidity. Long-range order increases with coating speed when the film thicknesses are kept constant, due to reduced nucleation per unit area of drying film. Fluorescence emission spectra of mCherry in films prepared at <25% relative humidity shows a small shift suggesting that proteins are more perturbed at low humidity than high humidity or the solution state.

Authors:
 [1];  [1];  [1]
  1. Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave Cambridge MA 02142 USA
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE
OSTI Identifier:
1339732
Resource Type:
Journal Article
Journal Name:
Macromolecular Rapid Communications
Additional Journal Information:
Journal Volume: 38; Journal Issue: 1; Journal ID: ISSN 1022-1336
Country of Publication:
United States
Language:
ENGLISH
Subject:
36 MATERIALS SCIENCE

Citation Formats

Chang, Dongsook, Huang, Aaron, and Olsen, Bradley D. Kinetic Effects on Self-Assembly and Function of Protein-Polymer Bioconjugates in Thin Films Prepared by Flow Coating. United States: N. p., 2016. Web. doi:10.1002/marc.201600449.
Chang, Dongsook, Huang, Aaron, & Olsen, Bradley D. Kinetic Effects on Self-Assembly and Function of Protein-Polymer Bioconjugates in Thin Films Prepared by Flow Coating. United States. https://doi.org/10.1002/marc.201600449
Chang, Dongsook, Huang, Aaron, and Olsen, Bradley D. 2016. "Kinetic Effects on Self-Assembly and Function of Protein-Polymer Bioconjugates in Thin Films Prepared by Flow Coating". United States. https://doi.org/10.1002/marc.201600449.
@article{osti_1339732,
title = {Kinetic Effects on Self-Assembly and Function of Protein-Polymer Bioconjugates in Thin Films Prepared by Flow Coating},
author = {Chang, Dongsook and Huang, Aaron and Olsen, Bradley D.},
abstractNote = {The self-assembly of nanostructured globular protein arrays in thin films is demonstrated using protein–polymer block copolymers based on a model protein mCherry and the polymer poly(oligoethylene glycol acrylate) (POEGA). Conjugates are flow coated into thin films on a poly(ethylene oxide) grafted Si surface, forming self-assembled cylindrical nanostructures with POEGA domains selectively segregating to the air–film interface. Long-range order and preferential arrangement of parallel cylinders templated by selective surfaces are demonstrated by controlling relative humidity. Long-range order increases with coating speed when the film thicknesses are kept constant, due to reduced nucleation per unit area of drying film. Fluorescence emission spectra of mCherry in films prepared at <25% relative humidity shows a small shift suggesting that proteins are more perturbed at low humidity than high humidity or the solution state.},
doi = {10.1002/marc.201600449},
url = {https://www.osti.gov/biblio/1339732}, journal = {Macromolecular Rapid Communications},
issn = {1022-1336},
number = 1,
volume = 38,
place = {United States},
year = {Fri Nov 04 00:00:00 EDT 2016},
month = {Fri Nov 04 00:00:00 EDT 2016}
}