The structure of Toho1 β‐lactamase in complex with penicillin reveals the role of Tyr105 in substrate recognition

Langan, Patricia S.; Vandavasi, Venu Gopal; Weiss, Kevin L.; Cooper, Jonathan B.; Ginell, Stephan L.; Coates, Leighton

doi:10.1002/2211-5463.12132

Title: The structure of Toho1 β‐lactamase in complex with penicillin reveals the role of Tyr105 in substrate recognition

Journal Article · Mon Nov 07 00:00:00 EST 2016 · FEBS Open Bio

DOI:https://doi.org/10.1002/2211-5463.12132· OSTI ID:1331023

Langan, Patricia S. ^[1]; Vandavasi, Venu Gopal ^[1]; Weiss, Kevin L. ^[1]; Cooper, Jonathan B. ^[2]; Ginell, Stephan L. ^[3]; Coates, Leighton ^[1]

Biology and Soft Matter Division Oak Ridge National Laboratory TN USA
Birkbeck University of London UK
Structural Biology Center Argonne National Laboratory IL USA

The role of the conserved residue Tyr105 in class A β‐lactamases has been the subject of investigation using both structural studies and saturation mutagenesis. Both have shown that while it does not need to be strictly conserved for activity, it is important for substrate recognition. With this in mind we determined the crystal structure of Toho1 β‐lactamase at 15 K to 1.10 Å resolution in complex with penicillin. As expected a ring‐opened penicillin molecule bound to Ser70 the catalytic nucleophile, can clearly be seen in electron density in the active site. In addition to the trapped penicillin, however, are two additional intact ring‐closed penicillin molecules, captured by the enzyme through noncovalent interactions at the edge of the active site.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1331023

Alternate ID(s):: OSTI ID: 1331025; OSTI ID: 1623459

Journal Information:: FEBS Open Bio, Journal Name: FEBS Open Bio Vol. 6 Journal Issue: 12; ISSN 2211-5463

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 9 works

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