N 2 gas is an effective fertilizer for bioethanol production by Zymomonas mobilis

Kremer, Timothy A.; LaSarre, Breah; Posto, Amanda L.; McKinlay, James B.

doi:10.1073/pnas.1420663112

Title: N ₂ gas is an effective fertilizer for bioethanol production by Zymomonas mobilis

Journal Article · Mon Feb 02 00:00:00 EST 2015 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1420663112· OSTI ID:1235509

Kremer, Timothy A. ^[1]; LaSarre, Breah ^[1]; Posto, Amanda L. ^[1]; McKinlay, James B. ^[1]

Indiana Univ., Bloomington, IN (United States). Dept. of Biology

A nascent cellulosic ethanol industry is struggling to become cost-competitive against corn ethanol and gasoline. Millions of dollars are spent on nitrogen supplements to make up for the low nitrogen content of the cellulosic feedstock. Here we show for the first time to our knowledge that the ethanol-producing bacterium, Zymomonas mobilis, can use N2 gas in lieu of traditional nitrogen supplements. Despite being an electron-intensive process, N2 fixation by Z. mobilis did not divert electrons away from ethanol production, as the ethanol yield was greater than 97% of the theoretical maximum. In a defined medium, Z. mobilis produced ethanol 50% faster per cell and generated half the unwanted biomass when supplied N2 instead of ammonium. In a cellulosic feedstock-derived medium, Z. mobilis achieved a similar cell density and a slightly higher ethanol yield when supplied N2 instead of the industrial nitrogen supplement, corn steep liquor. We estimate that N2-utilizing Z. mobilis could save a cellulosic ethanol production facility more than $1 million/y

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Research Organization:: Indiana Univ., Bloomington, IN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Contributing Organization:: Univ. of Illinois at Urbana-Champaign, IL (United States)

Grant/Contract Number:: SC0008131

OSTI ID:: 1235509

Alternate ID(s):: OSTI ID: 1348418; OSTI ID: 1454419

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Vol. 112 Journal Issue: 7; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 50 works

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References (32)

Biochemical Production of Ethanol from Corn Stover: 2008 State of Technology Model Humbird, D.; Aden, A. https://doi.org/10.2172/962490	report	August 2009
Calvin Cycle Mutants of Photoheterotrophic Purple Nonsulfur Bacteria Fail To Grow Due to an Electron Imbalance Rather than Toxic Metabolite Accumulation Gordon, G. C.; McKinlay, J. B. Journal of Bacteriology, Vol. 196, Issue 6 https://doi.org/10.1128/JB.01299-13	journal	January 2014
Cellulosic ethanol fights for life Peplow, Mark Nature, Vol. 507, Issue 7491 https://doi.org/10.1038/507152a	journal	March 2014
Production of Ethanol from Cellulosic Biomass Hydrolysates Using Genetically Engineered <I>Saccharomyces</I> Yeast Capable of Cofermenting Glucose and Xylose Sedlak, Miroslav; Ho, Nancy W. Y. Applied Biochemistry and Biotechnology, Vol. 114, Issue 1-3 https://doi.org/10.1385/ABAB:114:1-3:403	journal	January 2004
Cellulosic ethanol at last? Service, R. F. Science, Vol. 345, Issue 6201 https://doi.org/10.1126/science.345.6201.1111	journal	September 2014
Insights into Actinobacillus succinogenes Fermentative Metabolism in a Chemically Defined Growth Medium McKinlay, J. B.; Zeikus, J. G.; Vieille, C. Applied and Environmental Microbiology, Vol. 71, Issue 11 https://doi.org/10.1128/AEM.71.11.6651-6656.2005	journal	November 2005
Non-growing Rhodopseudomonas palustris Increases the Hydrogen Gas Yield from Acetate by Shifting from the Glyoxylate Shunt to the Tricarboxylic Acid Cycle McKinlay, James B.; Oda, Yasuhiro; Rühl, Martin Journal of Biological Chemistry, Vol. 289, Issue 4 https://doi.org/10.1074/jbc.M113.527515	journal	December 2013
Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol: Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover Humbird, D.; Davis, R.; Tao, L. https://doi.org/10.2172/1013269	report	March 2011
Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations Yang, Shihui; Tschaplinski, Timothy J.; Engle, Nancy L. BMC Genomics, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2164-10-34	journal	January 2009
Corn steep liquor as a cost-effective nutrition adjunct in high-performanceZymomonas ethanol fermentations Lawford, Hugh G.; Rousseau, Joyce D. Applied Biochemistry and Biotechnology, Vol. 63-65, Issue 1 https://doi.org/10.1007/BF02920431	journal	March 1997
Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis for Corn Stover Aden, A.; Ruth, M.; Ibsen, K. https://doi.org/10.2172/15001119 NREL/TP-510-32438	report	June 2002
Analysis of the Respiratory Chain in Ethanologenic <i>Zymomonas mobilis</i> with a Cyanide-Resistant <i>bd</i>-Type Ubiquinol Oxidase as the Only Terminal Oxidase and Its Possible Physiological Roles Sootsuwan, Kaewta; Lertwattanasakul, Noppon; Thanonkeo, Pornthap Journal of Molecular Microbiology and Biotechnology, Vol. 14, Issue 4 https://doi.org/10.1159/000112598	journal	January 2008
Carbon dioxide fixation as a central redox cofactor recycling mechanism in bacteria McKinlay, J. B.; Harwood, C. S. Proceedings of the National Academy of Sciences, Vol. 107, Issue 26 https://doi.org/10.1073/pnas.1006175107	journal	June 2010
Miscanthus : a fast-growing crop for biofuels and chemicals production : A fast-growing crop for biofuels and chemicals production Brosse, Nicolas; Dufour, Anthony; Meng, Xianzhi Biofuels, Bioproducts and Biorefining, Vol. 6, Issue 5 https://doi.org/10.1002/bbb.1353	journal	July 2012
Biochemical Production of Ethanol from Corn Stover: 2007 State of Technology Model Aden, Andy https://doi.org/10.2172/1218322	report	May 2008
Genome Sequence of the Ethanol-Producing Zymomonas mobilis subsp. pomaceae Lectotype Strain ATCC 29192 Kouvelis, V. N.; Davenport, K. W.; Brettin, T. S. Journal of Bacteriology, Vol. 193, Issue 18 https://doi.org/10.1128/JB.05273-11	journal	July 2011
Determining Actinobacillus succinogenes metabolic pathways and fluxes by NMR and GC-MS analyses of 13C-labeled metabolic product isotopomers McKinlay, James B.; Shachar-Hill, Yair; Zeikus, J. Gregory Metabolic Engineering, Vol. 9, Issue 2 https://doi.org/10.1016/j.ymben.2006.10.006	journal	March 2007
Modelling of the metabolism of Zymomonas mobilis growing on a defined medium Posten, C. Bioprocess Engineering, Vol. 4, Issue 5 https://doi.org/10.1007/BF00369175	journal	January 1989
Ethanol fermentation on the move Jeffries, Thomas W. Nature Biotechnology, Vol. 23, Issue 1 https://doi.org/10.1038/nbt0105-40	journal	January 2005
Genetic regulation of biological nitrogen fixation Dixon, Ray; Kahn, Daniel Nature Reviews Microbiology, Vol. 2, Issue 8 https://doi.org/10.1038/nrmicro954	journal	August 2004
Grain and cellulosic ethanol: History, economics, and energy policy Solomon, Barry D.; Barnes, Justin R.; Halvorsen, Kathleen E. Biomass and Bioenergy, Vol. 31, Issue 6 https://doi.org/10.1016/j.biombioe.2007.01.023	journal	June 2007
IsoCor: correcting MS data in isotope labeling experiments Millard, P.; Letisse, F.; Sokol, S. Bioinformatics, Vol. 28, Issue 9 https://doi.org/10.1093/bioinformatics/bts127	journal	March 2012
The genome-scale metabolic network analysis of Zymomonas mobilis ZM4 explains physiological features and suggests ethanol and succinic acid production strategies Lee, Kyung; Park, Jong; Kim, Tae Microbial Cell Factories, Vol. 9, Issue 1 https://doi.org/10.1186/1475-2859-9-94	journal	January 2010
13CFLUX2—high-performance software suite for 13C-metabolic flux analysis Weitzel, Michael; Nöh, Katharina; Dalman, Tolga Bioinformatics, Vol. 29, Issue 1 https://doi.org/10.1093/bioinformatics/bts646	journal	October 2012
Bioenergy crop greenhouse gas mitigation potential under a range of management practices Hudiburg, Tara W.; Davis, Sarah C.; Parton, William GCB Bioenergy, Vol. 7, Issue 2 https://doi.org/10.1111/gcbb.12152	journal	March 2014
How a century of ammonia synthesis changed the world Erisman, Jan Willem; Sutton, Mark A.; Galloway, James Nature Geoscience, Vol. 1, Issue 10 https://doi.org/10.1038/ngeo325	journal	September 2008
Kinetic studies of pigment synthesis by non-sulfur purple bacteria Cohen-Bazire, Germaine; Sistrom, W. R.; Stanier, R. Y. Journal of Cellular and Comparative Physiology, Vol. 49, Issue 1 https://doi.org/10.1002/jcp.1030490104	journal	February 1957
How can increased use of biological N ₂ fixation in agriculture benefit the environment? Jensen, Erik Steen; Hauggaard-Nielsen, Henrik Plant and Soil, Vol. 252, Issue 1 https://doi.org/10.1023/A:1024189029226	journal	May 2003
Critical analysis of techno-economic estimates for the production cost of lignocellulosic bio-ethanol Chovau, Simon; Degrauwe, David; Van der Bruggen, Bart Renewable and Sustainable Energy Reviews, Vol. 26 https://doi.org/10.1016/j.rser.2013.05.064	journal	October 2013
Physiology of Zymomonas mobilis: Some Unanswered Questions Kalnenieks, Uldis Advances in Microbial Physiology Volume 51 https://doi.org/10.1016/S0065-2911(06)51002-1	book	September 2006
Kinetic control of ethanol production by Zymomonas mobilis Jones, ColinW.; Doelle, HorstW. Applied Microbiology and Biotechnology, Vol. 35, Issue 1 https://doi.org/10.1007/BF00180626	journal	April 1991
Mitochondrial Metabolism in Developing Embryos of Brassica napus Schwender, Jörg; Shachar-Hill, Yair; Ohlrogge, John B. Journal of Biological Chemistry, Vol. 281, Issue 45 https://doi.org/10.1074/jbc.M606266200	journal	September 2006

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59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
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ethanol
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Title: N 2 gas is an effective fertilizer for bioethanol production by Zymomonas mobilis

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Title: N ₂ gas is an effective fertilizer for bioethanol production by Zymomonas mobilis