Metabolic engineering of Zymomonas mobilis for ethanol production from renewable feedstocks
Conference
·
OSTI ID:127024
- National Renewable Energy Lab., Golden, CO (United States); and others
Ethanol is an important industrial chemical and a national effort directed towards its development as an alternative transportation fuel will be successful if it can be produced from renewable feedstocks at economical cost. These feedstocks, such as agricultural and forestry residues, typically contain cellulose, hemicellulose and lignin. Unfortunately, conversion of pentoses sugars in the hemicellulose fraction, and particularly xylose, has been difficult due to the lack of a suitable biocatalyst. Zymomonas mobilis has demonstrated several advantages for ethanol production from glucose but is unable to ferment xylose due to the lack of xylose assimilation and pentose metabolism pathways. To broaden its range of fermentable sugars, we have metabolically engineered Z. mobilis to grow on and efficiently ferment xylose to ethanol by introducing the required metabolic pathways. The engineered strain efficiently ferments both glucose and xylose, which is essential for economical conversion of lignocellulosic biomass to ethanol.
- OSTI ID:
- 127024
- Report Number(s):
- CONF-950402--
- Country of Publication:
- United States
- Language:
- English
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