The cadmium-induced disruption of tight junctions in LLC-PK{sub 1} cells does not result from apoptosis
- Midwestern Univ., Downers Grove, IL (United States)
Exposure of LLC-PK{sub 1} cells to low micromolar concentrations of Cd{sup 2+} for 1-4 hours causes the disruption of the adhering and occluding junctions between the cells, whereas exposure to higher concentrations of Cd{sup 2+} for longer periods of time causes more severe toxic effects and cell death. The objective of the present studies was to determine whether or not the junctional effects of Cd{sup 2+} might be a consequence of apoptotic injury. LLC-PK{sub 1} cells on cell culture inserts were exposed to either Cd{sup +2} or tumor necrosis factor (TNF-{alpha}) plus cycloheximide, a treatment that has recently been shown to cause apoptosis in LLC-PK{sub 1} cells. The results showed that at the time the Cd{sup 2+}-induced junctional changes were occurring, there was no increase in the number of apoptotic cells or evidence of DNA fragmentation. By contrast, TNF-{alpha} plus cycloheximide induced changes that were characteristic of apoptosis. These results indicate that the disruption of intercellular junctions by Cd{sup 2+} in the LLC-PK{sub 1} cell line occurs independently of apoptosis. 19 refs., 3 figs.
- OSTI ID:
- 121290
- Journal Information:
- Life Sciences, Vol. 57, Issue 15; Other Information: PBD: 1 Sep 1995
- Country of Publication:
- United States
- Language:
- English
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