Comparison of the cytotoxic effects of cadmium chloride and cadmium-metallothionein in LLC-PK[sub 1] cells

Journal Article · Fri Jan 01 04:00:00 EST 1993 · Life Sciences; (United States)

OSTI ID:5100827

Prozialeck, W C; Wellington, D R; Lamar, P C ^[1]

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1. Midwestern Univ., Downers Grove, IL (United States)

Recent studies have shown that ionic cadmium (Cd[sup 2+]) can selectively damage the tight junctions between LLC-PK[sub 1] cells. The objective of the present studies was to determine if cadmium that is bound to metallothionein (CD-MT) can also damage the junctions between these cells. Cells on Falcon Cell Culture Inserts were exposed to Cd[sup 2+] or Cd-Mt from the apical and basolateral compartments. The integrity of cell junctions was assessed by monitoring the transepithelial electrical resistance, and cell viability was evaluated by monitoring the release of lactate dehydrogenase into the medium. Exposure to Cd[sup 2+] for 1-4 hours caused a pronounced decrease in the transepithelial resistance without affecting cell viability. By contrast, exposure to Cd-Mt had little effect on the electrical resistance until the cells began to die, which did not occur until 24-48 hours of exposure. Additional results showed that the cells accumulated Cd[sup 2+] more rapidly than Cd-Mt. These results indicate that Cd-Mt does not damage the junctions between LLC-PK[sub 1] cells, but that it can kill the cells after prolonged exposure.

• OSTI ID: 5100827
• Journal Information: Life Sciences; (United States), Journal Name: Life Sciences; (United States) Vol. 53:20; ISSN 0024-3205; ISSN LIFSAK
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
CADMIUM
CADMIUM COMPLEXES
CELL KILLING
COMPLEXES
ELEMENTS
JUNCTIONS
METALS