Review of methods to probe single cell metabolism and bioenergetics

Vasdekis, Andreas E.; Stephanopoulos, Gregory

doi:10.1016/j.ymben.2014.09.007

Title: Review of methods to probe single cell metabolism and bioenergetics

Journal Article · Fri Oct 31 00:00:00 EDT 2014 · Metabolic Engineering

DOI:https://doi.org/10.1016/j.ymben.2014.09.007· OSTI ID:1184963

Vasdekis, Andreas E. ^[1]; Stephanopoulos, Gregory ^[2]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab.
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Chemical Engineering

The sampling and manipulation of cells down to the individual has been of substantial interest since the very beginning of Life Sciences. Herein, our objective is to highlight the most recent developments in single cell manipulation, as well as pioneering ones. First, flow-through methods will be discussed, namely methods in which the single cells flow continuously in an ordered manner during their analysis. This section will be followed by confinement techniques that enable cell isolation and confinement in one, two- or three-dimensions. Flow cytometry and droplet microfluidics are the two most common methods of flow-through analysis. While both are high-throughput techniques, their difference lays in the fact that the droplet encapsulated cells experience a restricted and personal microenvironment, while in flow cytometry cells experience similar nutrient and stimuli initial concentrations. These methods are rather well established; however, they recently enabled immense strides in single cell phenotypic analysis, namely the identification and analysis of metabolically distinct individuals from an isogenic population using both droplet microfluidics and flow cytometry.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830; SC0008744; PN12005/2406

OSTI ID:: 1184963

Alternate ID(s):: OSTI ID: 1226845

Report Number(s):: PNNL-SA-104442

Journal Information:: Metabolic Engineering, Vol. 27, Issue C; ISSN 1096-7176

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 66 works

Citation information provided by
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