A fully-integrated aptamer-based affinity assay platform for monitoring astronaut health in space.
- AM Biotechnologies LLC, Houston, TX
Here we demonstrate the suitability of robust nucleic acid affinity reagents in an integrated point-of-care diagnostic platform for monitoring proteomic biomarkers indicative of astronaut health in spaceflight applications. A model thioaptamer targeting nuclear factor-kappa B (NF-{kappa}B) is evaluated in an on-chip electrophoretic gel-shift assay for human serum. Key steps of (i) mixing sample with the aptamer, (ii) buffer exchange, and (iii) preconcentration of sample were successfully integrated upstream of fluorescence-based detection. Challenges due to (i) nonspecific interactions with serum, and (ii) preconcentration at a nanoporous membrane are discussed and successfully resolved to yield a robust, rapid, and fully-integrated diagnostic system.
- Research Organization:
- Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC04-94AL85000
- OSTI ID:
- 1021620
- Report Number(s):
- SAND2010-4882C; TRN: US201117%%214
- Resource Relation:
- Conference: Proposed for presentation at the MicroTAS 2010 Conference held October 3-7, 2010 in Groningen, Netherlands.
- Country of Publication:
- United States
- Language:
- English
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