Selection of DNA aptamers against epidermal growth factor receptor with high affinity and specificity

Journal Article · Fri Oct 31 04:00:00 EDT 2014 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2014.09.023· OSTI ID:22416809

Wang, Deng-Liang ^[1]; Song, Yan-Ling; Zhu, Zhi; Li, Xi-Lan; Zou, Yuan ^[2]; Yang, Hai-Tao; Wang, Jiang-Jie ^[1]; Yao, Pei-Sen ^[3]; Pan, Ru-Jun ^[1]; Kang, De-Zhi ^[1]

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1. The First Clinical Medical College of Fujian Medical University, Fuzhou (China)
2. State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
3. Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou (China)

Highlights: • This is the first report of DNA aptamer against EGFR in vitro. • Aptamer can bind targets with high affinity and selectivity. • DNA aptamers are more stable, cheap and efficient than RNA aptamers. • Our selected DNA aptamer against EGFR has high affinity with K{sub d} 56 ± 7.3 nM. • Our selected DNA aptamer against EGFR has high selectivity. - Abstract: Epidermal growth factor receptor (EGFR/HER1/c-ErbB1), is overexpressed in many solid cancers, such as epidermoid carcinomas, malignant gliomas, etc. EGFR plays roles in proliferation, invasion, angiogenesis and metastasis of malignant cancer cells and is the ideal antigen for clinical applications in cancer detection, imaging and therapy. Aptamers, the output of the systematic evolution of ligands by exponential enrichment (SELEX), are DNA/RNA oligonucleotides which can bind protein and other substances with specificity. RNA aptamers are undesirable due to their instability and high cost of production. Conversely, DNA aptamers have aroused researcher’s attention because they are easily synthesized, stable, selective, have high binding affinity and are cost-effective to produce. In this study, we have successfully identified DNA aptamers with high binding affinity and selectivity to EGFR. The aptamer named TuTu22 with K{sub d} 56 ± 7.3 nM was chosen from the identified DNA aptamers for further study. Flow cytometry analysis results indicated that the TuTu22 aptamer was able to specifically recognize a variety of cancer cells expressing EGFR but did not bind to the EGFR-negative cells. With all of the aforementioned advantages, the DNA aptamers reported here against cancer biomarker EGFR will facilitate the development of novel targeted cancer detection, imaging and therapy.

• OSTI ID: 22416809
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 453; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AFFINITY
ANGIOGENESIS
ANTIGENS
BIOLOGICAL MARKERS
CARCINOMAS
CELL PROLIFERATION
GLIOMAS
GROWTH FACTORS
IN VITRO
LUNGS
METASTASES
NUCLEOTIDES
OLIGONUCLEOTIDES
RECEPTORS
RNA
THERAPY
TUMOR CELLS
TYROSINE