Melanization slows the rapid movement of fungal necromass carbon and nitrogen into both bacterial and fungal decomposer communities and soils
Abstract
Microbial necromass contributes significantly to both soil carbon (C) persistence and ecosystem nitrogen (N) availability, but quantitative estimates of C and N movement from necromass into soils and decomposer communities are lacking. Additionally, while melanin is known to slow fungal necromass decomposition, how it influences microbial C and N acquisition as well as elemental release into soils remains unclear. Here, we tracked decomposition of isotopically labeled low and high melanin fungal necromass and measured 13C and 15N accumulation in surrounding soils and microbial communities over 77 d in a temperate forest in Minnesota, USA. Mass loss was significantly higher from low melanin necromass, corresponding with greater 13C and 15N soil inputs. A taxonomically and functionally diverse array of bacteria and fungi was enriched in 13C and/or 15N at all sampling points, with enrichment being consistently higher on low melanin necromass and earlier in decomposition. Similar patterns of preferential C and N enrichment of many bacterial and fungal genera early in decomposition suggest that both microbial groups co-contribute to the rapid assimilation of resource-rich soil organic matter inputs. While overall richness of taxa enriched in C was higher than in N for both bacteria and fungi, there was a significant positivemore »
- Authors:
-
[3];
[1]
- University of Minnesota, Saint Paul, MN (United States)
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States); Univ. of California, Merced, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 2204112
- Report Number(s):
- LLNL-JRNL-852819
Journal ID: ISSN 2379-5077; 1080387
- Grant/Contract Number:
- AC52-07NA27344
- Resource Type:
- Accepted Manuscript
- Journal Name:
- mSystems
- Additional Journal Information:
- Journal Volume: 8; Journal Issue: 4; Journal ID: ISSN 2379-5077
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; bacteria; fungi; soil carbon and nitrogen cycling; forests; quantitative stable-isotope probing
Citation Formats
Maillard, François, Michaud, Talia J., See, Craig R., DeLancey, Lang C., Blazewicz, Steven J., Kimbrel, Jeffrey A., Pett-Ridge, Jennifer, and Kennedy, Peter G. Melanization slows the rapid movement of fungal necromass carbon and nitrogen into both bacterial and fungal decomposer communities and soils. United States: N. p., 2023.
Web. doi:10.1128/msystems.00390-23.
Maillard, François, Michaud, Talia J., See, Craig R., DeLancey, Lang C., Blazewicz, Steven J., Kimbrel, Jeffrey A., Pett-Ridge, Jennifer, & Kennedy, Peter G. Melanization slows the rapid movement of fungal necromass carbon and nitrogen into both bacterial and fungal decomposer communities and soils. United States. https://doi.org/10.1128/msystems.00390-23
Maillard, François, Michaud, Talia J., See, Craig R., DeLancey, Lang C., Blazewicz, Steven J., Kimbrel, Jeffrey A., Pett-Ridge, Jennifer, and Kennedy, Peter G. Tue .
"Melanization slows the rapid movement of fungal necromass carbon and nitrogen into both bacterial and fungal decomposer communities and soils". United States. https://doi.org/10.1128/msystems.00390-23. https://www.osti.gov/servlets/purl/2204112.
@article{osti_2204112,
title = {Melanization slows the rapid movement of fungal necromass carbon and nitrogen into both bacterial and fungal decomposer communities and soils},
author = {Maillard, François and Michaud, Talia J. and See, Craig R. and DeLancey, Lang C. and Blazewicz, Steven J. and Kimbrel, Jeffrey A. and Pett-Ridge, Jennifer and Kennedy, Peter G.},
abstractNote = {Microbial necromass contributes significantly to both soil carbon (C) persistence and ecosystem nitrogen (N) availability, but quantitative estimates of C and N movement from necromass into soils and decomposer communities are lacking. Additionally, while melanin is known to slow fungal necromass decomposition, how it influences microbial C and N acquisition as well as elemental release into soils remains unclear. Here, we tracked decomposition of isotopically labeled low and high melanin fungal necromass and measured 13C and 15N accumulation in surrounding soils and microbial communities over 77 d in a temperate forest in Minnesota, USA. Mass loss was significantly higher from low melanin necromass, corresponding with greater 13C and 15N soil inputs. A taxonomically and functionally diverse array of bacteria and fungi was enriched in 13C and/or 15N at all sampling points, with enrichment being consistently higher on low melanin necromass and earlier in decomposition. Similar patterns of preferential C and N enrichment of many bacterial and fungal genera early in decomposition suggest that both microbial groups co-contribute to the rapid assimilation of resource-rich soil organic matter inputs. While overall richness of taxa enriched in C was higher than in N for both bacteria and fungi, there was a significant positive relationship between C and N in co-enriched taxa. Collectively, our results demonstrate that melanization acts as a key ecological trait mediating not only fungal necromass decomposition rate but also necromass C and N release and that both elements are rapidly co-utilized by diverse bacterial and fungal decomposers in natural settings.},
doi = {10.1128/msystems.00390-23},
journal = {mSystems},
number = 4,
volume = 8,
place = {United States},
year = {Tue Jun 20 00:00:00 EDT 2023},
month = {Tue Jun 20 00:00:00 EDT 2023}
}
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