Arbuscular mycorrhizal fungal community responses to drought and nitrogen fertilization in switchgrass stands
Abstract
Anthropogenic global change is increasing the severity and frequency of abiotic stresses such as drought that are likely to affect soil communities. Arbuscular mycorrhizal fungi (AMF) play important roles in many soil processes, so it is important to understand how drought affects AMF biodiversity. This is especially relevant in agricultural systems where crops rely on AMF associations for water and nutrient uptake, and where management decisions such as crop selection and fertilizer application may influence how the AMF community responds to drought. In this study, we examined the effects of reduced precipitation and nitrogen fertilization on AMF richness, community composition, and root and soil colonization in monocultures of two cultivars of switchgrass (Panicum virgatum) grown for bioenergy feedstock. We conducted a two-year field experiment using rain-out shelters to manipulate precipitation in mature stands of switchgrass growing in a long-term nitrogen fertilization (0 or 56 kg N ha–1) experiment at the W.K. Kellogg Biological Station Long-Term Ecological Research site in Michigan, USA. We expected that AMF richness and colonization would decrease due to drought, as predicted by the stress exclusion hypothesis. Contrary to our expectations, we found that drought stress increased AMF species richness in fertilized plots by 15%; there wasmore »
- Authors:
-
- University of Louisville, KY (United States)
- Michigan State University, Hickory Corners, MI (United States)
- Michigan State University, Hickory Corners, MI (United States); University of Wisconsin-Green Bay, WI (United States)
- Michigan State University, Hickory Corners, MI (United States); Michigan State University, East Lansing, MI (United States)
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States); Battelle Memorial Institute, Columbus, OH (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1976865
- Grant/Contract Number:
- FC02-07ER64494; AC05-76RL01830; AC06-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Applied Soil Ecology
- Additional Journal Information:
- Journal Volume: 169; Journal Issue: C; Journal ID: ISSN 0929-1393
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; Agriculture; Drought; Nitrogen; Arbuscular mycorrhizal fungi; Biofuels; Long-term ecological research; Sequencing
Citation Formats
Emery, Sarah M., Bell-Dereske, Lukas, Stahlheber, Karen A., and Gross, Katherine L. Arbuscular mycorrhizal fungal community responses to drought and nitrogen fertilization in switchgrass stands. United States: N. p., 2021.
Web. doi:10.1016/j.apsoil.2021.104218.
Emery, Sarah M., Bell-Dereske, Lukas, Stahlheber, Karen A., & Gross, Katherine L. Arbuscular mycorrhizal fungal community responses to drought and nitrogen fertilization in switchgrass stands. United States. https://doi.org/10.1016/j.apsoil.2021.104218
Emery, Sarah M., Bell-Dereske, Lukas, Stahlheber, Karen A., and Gross, Katherine L. Wed .
"Arbuscular mycorrhizal fungal community responses to drought and nitrogen fertilization in switchgrass stands". United States. https://doi.org/10.1016/j.apsoil.2021.104218. https://www.osti.gov/servlets/purl/1976865.
@article{osti_1976865,
title = {Arbuscular mycorrhizal fungal community responses to drought and nitrogen fertilization in switchgrass stands},
author = {Emery, Sarah M. and Bell-Dereske, Lukas and Stahlheber, Karen A. and Gross, Katherine L.},
abstractNote = {Anthropogenic global change is increasing the severity and frequency of abiotic stresses such as drought that are likely to affect soil communities. Arbuscular mycorrhizal fungi (AMF) play important roles in many soil processes, so it is important to understand how drought affects AMF biodiversity. This is especially relevant in agricultural systems where crops rely on AMF associations for water and nutrient uptake, and where management decisions such as crop selection and fertilizer application may influence how the AMF community responds to drought. In this study, we examined the effects of reduced precipitation and nitrogen fertilization on AMF richness, community composition, and root and soil colonization in monocultures of two cultivars of switchgrass (Panicum virgatum) grown for bioenergy feedstock. We conducted a two-year field experiment using rain-out shelters to manipulate precipitation in mature stands of switchgrass growing in a long-term nitrogen fertilization (0 or 56 kg N ha–1) experiment at the W.K. Kellogg Biological Station Long-Term Ecological Research site in Michigan, USA. We expected that AMF richness and colonization would decrease due to drought, as predicted by the stress exclusion hypothesis. Contrary to our expectations, we found that drought stress increased AMF species richness in fertilized plots by 15%; there was no effect of drought on AMF richness in unfertilized plots. Drought also significantly altered AMF community composition, primarily due to increases in Rhizophagus taxa abundance, and reduced AMF root colonization in switchgrass by 6%. We also found variation in AMF richness and colonization across switchgrass cultivars as well as sampling dates. Furthermore, the changes in AMF richness and composition that we observed in this study may have implications for perennial bioenergy feedstock selection and management as changes in AMF communities may feedback to affect host plants.},
doi = {10.1016/j.apsoil.2021.104218},
journal = {Applied Soil Ecology},
number = C,
volume = 169,
place = {United States},
year = {Wed Sep 08 00:00:00 EDT 2021},
month = {Wed Sep 08 00:00:00 EDT 2021}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Biomass Yield and Stand Characteristics of Switchgrass in South Central U.S. Environments
journal, January 2005
- Cassida, K. A.; Muir, J. P.; Hussey, M. A.
- Crop Science, Vol. 45, Issue 2
Effects of different management practices on arbuscular mycorrhizal fungal diversity in maize fields by a molecular approach
journal, April 2012
- Borriello, Roberto; Lumini, Erica; Girlanda, Mariangela
- Biology and Fertility of Soils, Vol. 48, Issue 8
Fungal community responses to precipitation: FUNGAL CLIMATE RESPONSE
journal, October 2010
- Hawkes, Christine V.; Kivlin, Stephanie N.; Rocca, Jennifer D.
- Global Change Biology, Vol. 17, Issue 4
Associations between species and groups of sites: indices and statistical inference
journal, December 2009
- Cáceres, Miquel De; Legendre, Pierre
- Ecology, Vol. 90, Issue 12
The potential role of arbuscular mycorrhizal (AM) fungi in the bioprotection of plants against soil-borne pathogens in organic and/or other sustainable farming systems
journal, January 2004
- Harrier, Lucy A.; Watson, Christine A.
- Pest Management Science, Vol. 60, Issue 2
Effects of long-term fertilization on AM fungal community structure and Glomalin-related soil protein in the Loess Plateau of China
journal, December 2010
- Wu, Fasi; Dong, Maoxing; Liu, Yongjun
- Plant and Soil, Vol. 342, Issue 1-2
Arbuscular mycorrhizal symbiosis and abiotic stress in plants: A review
journal, October 2016
- Latef, Arafat Abdel Hamed Abdel; Hashem, Abeer; Rasool, Saiema
- Journal of Plant Biology, Vol. 59, Issue 5
Soil mycorrhizal and nematode diversity vary in response to bioenergy crop identity and fertilization
journal, June 2017
- Emery, Sarah M.; Reid, Matthew L.; Bell-Dereske, Lukas
- GCB Bioenergy, Vol. 9, Issue 11
A meta-analysis of mycorrhizal responses to nitrogen, phosphorus, and atmospheric CO2 in field studies
journal, July 2004
- Treseder, Kathleen K.
- New Phytologist, Vol. 164, Issue 2
Feedstock loss from drought is a major economic risk for biofuel producers
journal, October 2014
- Morrow, William R.; Gopal, Anand; Fitts, Gary
- Biomass and Bioenergy, Vol. 69
Global negative effects of nitrogen deposition on soil microbes
journal, March 2018
- Zhang, Tian’an; Chen, Han Y. H.; Ruan, Honghua
- The ISME Journal, Vol. 12, Issue 7
A rainout shelter design for intercepting different amounts of rainfall
journal, October 2002
- Yahdjian, Laura; Sala, Osvaldo E.
- Oecologia, Vol. 133, Issue 2
Arbuscular mycorrhizal fungi and organic farming
journal, April 2006
- Gosling, P.; Hodge, A.; Goodlass, G.
- Agriculture, Ecosystems & Environment, Vol. 113, Issue 1-4
Waste Not, Want Not: Why Rarefying Microbiome Data Is Inadmissible
journal, April 2014
- McMurdie, Paul J.; Holmes, Susan
- PLoS Computational Biology, Vol. 10, Issue 4
Naive Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy
journal, June 2007
- Wang, Q.; Garrity, G. M.; Tiedje, J. M.
- Applied and Environmental Microbiology, Vol. 73, Issue 16
Belowground biodiversity and ecosystem functioning
journal, November 2014
- Bardgett, Richard D.; van der Putten, Wim H.
- Nature, Vol. 515, Issue 7528
Evolutionary ecology of mycorrhizal functional diversity in agricultural systems: AMF in agriculture
journal, July 2010
- Verbruggen, Erik; Toby Kiers, E.
- Evolutionary Applications, Vol. 3, Issue 5-6
Phylogenetic trait conservatism and the evolution of functional trade-offs in arbuscular mycorrhizal fungi
journal, September 2009
- Powell, Jeff R.; Parrent, Jeri L.; Hart, Miranda M.
- Proceedings of the Royal Society B: Biological Sciences, Vol. 276, Issue 1676
Soil microbial communities under model biofuel cropping systems in southern Wisconsin, USA: Impact of crop species and soil properties
journal, March 2012
- Liang, Chao; Jesus, Ederson da C.; Duncan, David S.
- Applied Soil Ecology, Vol. 54
Arbuscular mycorrhizal fungal communities and global change: an uncertain future
journal, September 2018
- Cotton, TE Anne
- FEMS Microbiology Ecology, Vol. 94, Issue 11
Mycorrhizal fungal abundance is affected by long-term climatic manipulations in the field
journal, February 2003
- Staddon, Philip L.; Thompson, Ken; Jakobsen, Iver
- Global Change Biology, Vol. 9, Issue 2
Arbuscular mycorrhizal fungal responses to abiotic stresses: A review
journal, March 2016
- Lenoir, Ingrid; Fontaine, Joël; Lounès-Hadj Sahraoui, Anissa
- Phytochemistry, Vol. 123
Fungal symbionts alter plant responses to global change
journal, July 2013
- Kivlin, Stephanie N.; Emery, Sarah M.; Rudgers, Jennifer A.
- American Journal of Botany, Vol. 100, Issue 7
Ribosomal Database Project: data and tools for high throughput rRNA analysis
journal, November 2013
- Cole, James R.; Wang, Qiong; Fish, Jordan A.
- Nucleic Acids Research, Vol. 42, Issue D1
Response of “Alamo” switchgrass tissue chemistry and biomass to nitrogen fertilization in West Tennessee, USA
journal, January 2011
- Garten, Charles T.; Brice, Deanne J.; Castro, Hector F.
- Agriculture, Ecosystems & Environment, Vol. 140, Issue 1-2
Low variation in arbuscular mycorrhizal fungal associations and effects on biomass among switchgrass cultivars
journal, December 2018
- Emery, Sarah M.; Kinnetz, Erin R.; Bell-Dereske, Lukas
- Biomass and Bioenergy, Vol. 119
An Evaluation of Techniques for Measuring Vesicular Arbuscular Mycorrhizal Infection in Roots
journal, March 1980
- Giovannetti, M.; Mosse, B.
- New Phytologist, Vol. 84, Issue 3
Observations on the extra-matrical mycelium of a vesicular-arbuscular endophyte
journal, December 1959
- Mosse, Barbara
- Transactions of the British Mycological Society, Vol. 42, Issue 4
Consistent responses of soil microbial communities to elevated nutrient inputs in grasslands across the globe
journal, August 2015
- Leff, Jonathan W.; Jones, Stuart E.; Prober, Suzanne M.
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 35
UPARSE: highly accurate OTU sequences from microbial amplicon reads
journal, August 2013
- Edgar, Robert C.
- Nature Methods, Vol. 10, Issue 10
Soil carbon mapping using on-the-go near infrared spectroscopy, topography and aerial photographs
journal, October 2011
- Muñoz, Juan D.; Kravchenko, Alexandra
- Geoderma, Vol. 166, Issue 1
Drought minimized nitrogen fertilization effects on bioenergy feedstock quality
journal, February 2020
- Emery, Sarah M.; Stahlheber, Karen A.; Gross, Katherine L.
- Biomass and Bioenergy, Vol. 133
A trait-based framework to understand life history of mycorrhizal fungi
journal, September 2013
- Chagnon, Pierre-Luc; Bradley, Robert L.; Maherali, Hafiz
- Trends in Plant Science, Vol. 18, Issue 9
Temporal dynamics of mycorrhizal fungal communities and co‐associations with grassland plant communities following experimental manipulation of rainfall
journal, September 2019
- Deveautour, Coline; Power, Sally A.; Barnett, Kirk L.
- Journal of Ecology, Vol. 108, Issue 2
Ink and Vinegar, a Simple Staining Technique for Arbuscular-Mycorrhizal Fungi
journal, January 1998
- Vierheilig, Horst; Coughlan, Andrew P.; Wyss, Urs
- Applied and Environmental Microbiology, Vol. 64, Issue 12
Registration of Southlow Michigan Germplasm Switchgrass
journal, January 2008
- Durling, J. C.; Leif, J. W.; Burgdorf, D. W.
- Journal of Plant Registrations, Vol. 2, Issue 1
Climate change impacts on biodiversity and ecosystems in Sri Lanka: a review
journal, January 2017
- Kottawa-Arachchi, J. Dananjaya; Wijeratne, Madawala A.
- Nature Conservation Research, Vol. 2, Issue 3
Patterns and drivers of biodiversity-stability relationships under climate extremes
journal, November 2017
- De Boeck, Hans J.; Bloor, Juliette M. G.; Kreyling, Juergen
- Journal of Ecology, Vol. 106, Issue 3
Effect of elevated atmospheric CO2 on mycorrhizal colonization, external mycorrhizal hyphal production and phosphorus inflow in Plantago lanceolata and Trifolium repens in association with the arbuscular mycorrhizal fungus Glomus mosseae
journal, March 1999
- Staddon, Philip L.; Fitter, Alastair H.; Graves, Jonathan D.
- Global Change Biology, Vol. 5, Issue 3
Climate change impacts on biodiversity in Switzerland: A review
journal, June 2013
- Vittoz, Pascal; Cherix, Daniel; Gonseth, Yves
- Journal for Nature Conservation, Vol. 21, Issue 3
Cultivar × Environment Interactions in Switchgrass
journal, January 2003
- Casler, M. D.; Boe, A. R.
- Crop Science, Vol. 43, Issue 6
More Intense, More Frequent, and Longer Lasting Heat Waves in the 21st Century
journal, August 2004
- Meehl, G. A.
- Science, Vol. 305, Issue 5686
An evidence-based consensus for the classification of arbuscular mycorrhizal fungi (Glomeromycota)
journal, April 2013
- Redecker, Dirk; Schüßler, Arthur; Stockinger, Herbert
- Mycorrhiza, Vol. 23, Issue 7
The abundance of arbuscular mycorrhizal fungal species in symbiosis with okra plants is affected by induced drought conditions in a calcareous substrate
journal, June 2019
- Müller, Anja; Eltigani, Amna; George, Eckhard
- Rhizosphere, Vol. 10
Mycorrhizal Community Dynamics Following Nitrogen Fertilization: a Cross-Site test in five Grasslands
journal, November 2007
- Egerton-Warburton, Louise M.; Johnson, Nancy Collins; Allen, Edith B.
- Ecological Monographs, Vol. 77, Issue 4
Comparative economic analysis of perennial, annual, and intercrops for biomass production
journal, December 2001
- Hallam, A.; Anderson, I. C.; Buxton, D. R.
- Biomass and Bioenergy, Vol. 21, Issue 6
Diversity of arbuscular mycorrhizal fungi colonising roots of the grass species Agrostis capillaris and Lolium perenne in a field experiment
journal, May 2003
- Gollotte, Armelle; van Tuinen, Diederik; Atkinson, David
- Mycorrhiza, Vol. 14, Issue 2
A meta-analysis of context-dependency in plant response to inoculation with mycorrhizal fungi
journal, March 2010
- Hoeksema, Jason D.; Chaudhary, V. Bala; Gehring, Catherine A.
- Ecology Letters, Vol. 13, Issue 3
Taxonomic shifts in arbuscular mycorrhizal fungal communities with shade and soil nitrogen across conventionally managed and organic coffee agroecosystems
journal, June 2020
- Aldrich-Wolfe, Laura; Black, Katie L.; Hartmann, Eliza D. L.
- Mycorrhiza, Vol. 30, Issue 4
Community Structure of Arbuscular Mycorrhizal Fungi in Soils of Switchgrass Harvested for Bioenergy
journal, September 2020
- Dirks, Alden C.; Jackson, Randall D.
- Applied and Environmental Microbiology, Vol. 86, Issue 19
Soil moisture—a regulator of arbuscular mycorrhizal fungal community assembly and symbiotic phosphorus uptake
journal, August 2014
- Deepika, Sharma; Kothamasi, David
- Mycorrhiza, Vol. 25, Issue 1
Little evidence that farmers should consider abundance or diversity of arbuscular mycorrhizal fungi when managing crops
journal, July 2018
- Ryan, Megan H.; Graham, James H.
- New Phytologist, Vol. 220, Issue 4
Plant communities mediate the interactive effects of invasion and drought on soil microbial communities
journal, February 2020
- Fahey, Catherine; Koyama, Akihiro; Antunes, Pedro M.
- The ISME Journal, Vol. 14, Issue 6
Stressed out symbiotes: hypotheses for the influence of abiotic stress on arbuscular mycorrhizal fungi
journal, June 2016
- Millar, Niall S.; Bennett, Alison E.
- Oecologia, Vol. 182, Issue 3
Tracking carbon from the atmosphere to the rhizosphere
journal, December 2005
- Olsson, Pal A.; Johnson, Nancy C.
- Ecology Letters, Vol. 8, Issue 12
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
journal, December 2014
- Love, Michael I.; Huber, Wolfgang; Anders, Simon
- Genome Biology, Vol. 15, Issue 12
Diversity of communities of arbuscular mycorrhizal (AM) fungi present in conventional versus low-input agricultural sites in eastern Pennsylvania, USA
journal, January 2001
- Franke-Snyder, Marlise; Douds, David D.; Galvez, Larisa
- Applied Soil Ecology, Vol. 16, Issue 1
The Biology and Agronomy of Switchgrass for Biofuels
journal, September 2005
- Parrish, David J.; Fike, John H.
- Critical Reviews in Plant Sciences, Vol. 24, Issue 5-6
Predicting productivity: A trait-based analysis of variability in biomass yield among switchgrass feedstock cultivars
journal, September 2020
- Stahlheber, Karen A.; Lindquist, Jacob; Drogosh, Peter D.
- Agriculture, Ecosystems & Environment, Vol. 300
Shifts in Arbuscular Mycorrhizal Communities Along an Anthropogenic Nitrogen Deposition Gradient
journal, April 2000
- Egerton-Warburton, Louise M.; Allen, Edith B.
- Ecological Applications, Vol. 10, Issue 2