Varroa destructor mites vector and transmit pathogenic honey bee viruses acquired from an artificial diet
Abstract
The ectoparasitic mite Varroa destructor is one of the most destructive pests of the honey bee (Apis mellifera) and the primary biotic cause of colony collapse in many regions of the world. These mites inflict physical injury on their honey bee hosts from feeding on host hemolymph and fat body cells/cellular components, and serve as the vector for deadly honey bee viruses, including Deformed wing virus (DWV) and the related Varroa destructor virus-1 (VDV-1) (i.e., DWV-like viruses). Studies focused on elucidating the dynamics of Varroa-mediated vectoring and transmission of DWV-like viruses may be confounded by viruses present in ingested host tissues or the mites themselves. Here we describe a system that includes an artificial diet free of insect tissue-derived components for maintaining Varroa mites for in vitro experimentation. Using this system, together with the novel engineered cDNA clone-derived genetically tagged VDV-1 and wild-type DWV, we demonstrated for the first time that Varroa mites provided an artificial diet supplemented with engineered viruses for 36 hours could acquire and transmit sufficient numbers of virus particles to establish an infection in virus-naïve hosts. While the in vitro system described herein provides for only up to five days of mite survival, precluding study ofmore »
- Authors:
-
[1];
[1]
- US Dept. of Agriculture (USDA), Beltsville, MD (United States)
- US Dept. of Agriculture (USDA), Beltsville, MD (United States); Old Dominion University, Norfolk, VA (United States)
- Publication Date:
- Research Org.:
- Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); National Institute of Food and Agriculture (NIFA); United States Department of Agriculture (USDA)
- OSTI Identifier:
- 1904924
- Grant/Contract Number:
- SC0014664; 2017-06481; 19-8130-0745-IA
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 15; Journal Issue: 11; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; mites; pupae; honey bees; diet; viral transmission and infection; trophic interactions; vector biology; viral vectors
Citation Formats
Posada-Florez, Francisco, Ryabov, Eugene V., Heerman, Matthew C., Chen, Yanping, Evans, Jay D., Sonenshine, Daniel E., and Cook, Steven C. Varroa destructor mites vector and transmit pathogenic honey bee viruses acquired from an artificial diet. United States: N. p., 2020.
Web. doi:10.1371/journal.pone.0242688.
Posada-Florez, Francisco, Ryabov, Eugene V., Heerman, Matthew C., Chen, Yanping, Evans, Jay D., Sonenshine, Daniel E., & Cook, Steven C. Varroa destructor mites vector and transmit pathogenic honey bee viruses acquired from an artificial diet. United States. https://doi.org/10.1371/journal.pone.0242688
Posada-Florez, Francisco, Ryabov, Eugene V., Heerman, Matthew C., Chen, Yanping, Evans, Jay D., Sonenshine, Daniel E., and Cook, Steven C. Tue .
"Varroa destructor mites vector and transmit pathogenic honey bee viruses acquired from an artificial diet". United States. https://doi.org/10.1371/journal.pone.0242688. https://www.osti.gov/servlets/purl/1904924.
@article{osti_1904924,
title = {Varroa destructor mites vector and transmit pathogenic honey bee viruses acquired from an artificial diet},
author = {Posada-Florez, Francisco and Ryabov, Eugene V. and Heerman, Matthew C. and Chen, Yanping and Evans, Jay D. and Sonenshine, Daniel E. and Cook, Steven C.},
abstractNote = {The ectoparasitic mite Varroa destructor is one of the most destructive pests of the honey bee (Apis mellifera) and the primary biotic cause of colony collapse in many regions of the world. These mites inflict physical injury on their honey bee hosts from feeding on host hemolymph and fat body cells/cellular components, and serve as the vector for deadly honey bee viruses, including Deformed wing virus (DWV) and the related Varroa destructor virus-1 (VDV-1) (i.e., DWV-like viruses). Studies focused on elucidating the dynamics of Varroa-mediated vectoring and transmission of DWV-like viruses may be confounded by viruses present in ingested host tissues or the mites themselves. Here we describe a system that includes an artificial diet free of insect tissue-derived components for maintaining Varroa mites for in vitro experimentation. Using this system, together with the novel engineered cDNA clone-derived genetically tagged VDV-1 and wild-type DWV, we demonstrated for the first time that Varroa mites provided an artificial diet supplemented with engineered viruses for 36 hours could acquire and transmit sufficient numbers of virus particles to establish an infection in virus-naïve hosts. While the in vitro system described herein provides for only up to five days of mite survival, precluding study of the long-term impacts of viruses on mite health, the system allows for extensive insights into the dynamics of Varroa-mediated vectoring and transmission of honey bee viruses.},
doi = {10.1371/journal.pone.0242688},
journal = {PLoS ONE},
number = 11,
volume = 15,
place = {United States},
year = {Tue Nov 24 00:00:00 EST 2020},
month = {Tue Nov 24 00:00:00 EST 2020}
}
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