Draft genome sequences of strains CBS6241 and CBS6242 of the basidiomycetous yeast Filobasidium floriforme
Abstract
The Tremellomycetes are a species-rich group within the basidiomycete fungi; however, most analyses of this group to date have focused on pathogenic Cryptococcus species within the order Tremellales. Recent genome-assisted studies of other Tremellomycetes have identified interesting features with respect to biotechnological applications as well as the evolution of genes involved in mating and sexual development. Here, we report genome sequences of two strains of Filobasidium floriforme, a species from the order Filobasidiales, which branches basally to the Tremellales, Trichosporonales, and Holtermanniales. The assembled genomes of strains CBS6241 and CBS6242 are 27.4 Mb and 26.4 Mb in size, respectively, with 8314 and 7695 predicted protein-coding genes. Overall sequence identity at nucleic acid level between the strains is 97%. Among the predicted genes are pheromone precursor and pheromone receptor genes as well as two genes encoding homedomain (HD) transcription factors, which are predicted to be part of the mating type (MAT) locus. Sequence analysis indicates that CBS6241 and CBS6242 carry different alleles for both the pheromone/receptor genes as well as the HD transcription factors. Orthology inference identified 1482 orthogroups exclusively found in F. floriforme, some of which were involved in carbohydrate transport and metabolism. Subsequent CAZyme repertoire characterization identified 267 andmore »
- Authors:
-
[1];
[1]
- Ruhr Univ., Bochum (Germany)
- USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); German Research Foundation (DFG)
- OSTI Identifier:
- 1894069
- Grant/Contract Number:
- AC02-05CH11231; NO407/7-2
- Resource Type:
- Accepted Manuscript
- Journal Name:
- G3
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 2; Journal ID: ISSN 2160-1836
- Publisher:
- Genetics Society of America
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Filobasidium floriforme; mating-type locus; basidiomycete; Filobasidiales; CAZymes
Citation Formats
Guerreiro, Marco Alexandre, Ahrendt, Steven, Pangilinan, Jasmyn, Chen, Cindy, Yan, Mi, Lipzen, Anna, Barry, Kerrie, Grigoriev, Igor V., Begerow, Dominik, and Nowrousian, Minou. Draft genome sequences of strains CBS6241 and CBS6242 of the basidiomycetous yeast Filobasidium floriforme. United States: N. p., 2021.
Web. doi:10.1093/g3journal/jkab398.
Guerreiro, Marco Alexandre, Ahrendt, Steven, Pangilinan, Jasmyn, Chen, Cindy, Yan, Mi, Lipzen, Anna, Barry, Kerrie, Grigoriev, Igor V., Begerow, Dominik, & Nowrousian, Minou. Draft genome sequences of strains CBS6241 and CBS6242 of the basidiomycetous yeast Filobasidium floriforme. United States. https://doi.org/10.1093/g3journal/jkab398
Guerreiro, Marco Alexandre, Ahrendt, Steven, Pangilinan, Jasmyn, Chen, Cindy, Yan, Mi, Lipzen, Anna, Barry, Kerrie, Grigoriev, Igor V., Begerow, Dominik, and Nowrousian, Minou. Mon .
"Draft genome sequences of strains CBS6241 and CBS6242 of the basidiomycetous yeast Filobasidium floriforme". United States. https://doi.org/10.1093/g3journal/jkab398. https://www.osti.gov/servlets/purl/1894069.
@article{osti_1894069,
title = {Draft genome sequences of strains CBS6241 and CBS6242 of the basidiomycetous yeast Filobasidium floriforme},
author = {Guerreiro, Marco Alexandre and Ahrendt, Steven and Pangilinan, Jasmyn and Chen, Cindy and Yan, Mi and Lipzen, Anna and Barry, Kerrie and Grigoriev, Igor V. and Begerow, Dominik and Nowrousian, Minou},
abstractNote = {The Tremellomycetes are a species-rich group within the basidiomycete fungi; however, most analyses of this group to date have focused on pathogenic Cryptococcus species within the order Tremellales. Recent genome-assisted studies of other Tremellomycetes have identified interesting features with respect to biotechnological applications as well as the evolution of genes involved in mating and sexual development. Here, we report genome sequences of two strains of Filobasidium floriforme, a species from the order Filobasidiales, which branches basally to the Tremellales, Trichosporonales, and Holtermanniales. The assembled genomes of strains CBS6241 and CBS6242 are 27.4 Mb and 26.4 Mb in size, respectively, with 8314 and 7695 predicted protein-coding genes. Overall sequence identity at nucleic acid level between the strains is 97%. Among the predicted genes are pheromone precursor and pheromone receptor genes as well as two genes encoding homedomain (HD) transcription factors, which are predicted to be part of the mating type (MAT) locus. Sequence analysis indicates that CBS6241 and CBS6242 carry different alleles for both the pheromone/receptor genes as well as the HD transcription factors. Orthology inference identified 1482 orthogroups exclusively found in F. floriforme, some of which were involved in carbohydrate transport and metabolism. Subsequent CAZyme repertoire characterization identified 267 and 247 enzymes for CBS6241 and CBS6242, respectively, the second highest number of CAZymes among the analyzed Tremellomycete species. In addition, F. floriforme contains five CAZymes absent in other species and several plant-cell-wall degrading CAZymes with the highest copy number in Tremellomycota, indicating the biotechnological potential of this species.},
doi = {10.1093/g3journal/jkab398},
journal = {G3},
number = 2,
volume = 12,
place = {United States},
year = {Mon Nov 15 00:00:00 EST 2021},
month = {Mon Nov 15 00:00:00 EST 2021}
}
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