Restoration and Modification of Magnetosome Biosynthesis by Internal Gene Acquisition in a Magnetotactic Bacterium
Abstract
We report that the Integration of a large-sized DNA fragment into a chromosome is an important strategy for characterization of cellular functions in microorganisms. Magnetotactic bacteria synthesize intracellular organelles comprising membrane-bound single crystalline magnetite, also referred to as magnetosomes. Magnetosomes have gained interest in both scientific and engineering sectors as they can be utilized as a material for biomedical and nanotechnological applications. Although genetic engineering of magnetosome biosynthesis mechanism has been investigated, the current method requires cumbersome gene preparation processes. Here, the chromosomal integration of a plasmid containing ≈27 magnetosome genes (≈26 kbp region) in a non-magnetic mutant of Magnetospirillum magneticum AMB-1 using a broad-host-range plasmid is shown. The genome sequencing of gene-complemented strains reveals the chromosomal integration of the plasmid with magnetosome genes at a specific site, most likely by catalysis of an endogenous transposase. Magnetosome production is successfully enhanced by integrating a variation of magnetosome gene operons in the chromosome. This chromosomal integration mechanism will allow the design of functional magnetosomes de novo and M. magneticum AMB-1 may be used as a chassis for the designed magnetosome production.
- Authors:
-
[2];
- Tokyo Univ. of Agriculture and Technology (Japan)
- Tokyo Institute of Technology (Japan)
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Tokyo Univ. of Agriculture and Technology (Japan); Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka (Japan)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); Japan Society for the Promotion of Science (JSPS)
- OSTI Identifier:
- 1845297
- Grant/Contract Number:
- AC02-05CH11231; 16H02421; 18H01794
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology Journal
- Additional Journal Information:
- Journal Volume: 15; Journal Issue: 12; Journal ID: ISSN 1860-6768
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; magnetotactic bacteria; genetic engineering; chromosomal integration; biomineralization; bioproduction
Citation Formats
Arakaki, Atsushi, Goto, Mayu, Maruyama, Mina, Yoda, Takuto, Tanaka, Masayoshi, Yamagishi, Ayana, Yoshikuni, Yasuo, and Matsunaga, Tadashi. Restoration and Modification of Magnetosome Biosynthesis by Internal Gene Acquisition in a Magnetotactic Bacterium. United States: N. p., 2020.
Web. doi:10.1002/biot.202000278.
Arakaki, Atsushi, Goto, Mayu, Maruyama, Mina, Yoda, Takuto, Tanaka, Masayoshi, Yamagishi, Ayana, Yoshikuni, Yasuo, & Matsunaga, Tadashi. Restoration and Modification of Magnetosome Biosynthesis by Internal Gene Acquisition in a Magnetotactic Bacterium. United States. https://doi.org/10.1002/biot.202000278
Arakaki, Atsushi, Goto, Mayu, Maruyama, Mina, Yoda, Takuto, Tanaka, Masayoshi, Yamagishi, Ayana, Yoshikuni, Yasuo, and Matsunaga, Tadashi. Wed .
"Restoration and Modification of Magnetosome Biosynthesis by Internal Gene Acquisition in a Magnetotactic Bacterium". United States. https://doi.org/10.1002/biot.202000278. https://www.osti.gov/servlets/purl/1845297.
@article{osti_1845297,
title = {Restoration and Modification of Magnetosome Biosynthesis by Internal Gene Acquisition in a Magnetotactic Bacterium},
author = {Arakaki, Atsushi and Goto, Mayu and Maruyama, Mina and Yoda, Takuto and Tanaka, Masayoshi and Yamagishi, Ayana and Yoshikuni, Yasuo and Matsunaga, Tadashi},
abstractNote = {We report that the Integration of a large-sized DNA fragment into a chromosome is an important strategy for characterization of cellular functions in microorganisms. Magnetotactic bacteria synthesize intracellular organelles comprising membrane-bound single crystalline magnetite, also referred to as magnetosomes. Magnetosomes have gained interest in both scientific and engineering sectors as they can be utilized as a material for biomedical and nanotechnological applications. Although genetic engineering of magnetosome biosynthesis mechanism has been investigated, the current method requires cumbersome gene preparation processes. Here, the chromosomal integration of a plasmid containing ≈27 magnetosome genes (≈26 kbp region) in a non-magnetic mutant of Magnetospirillum magneticum AMB-1 using a broad-host-range plasmid is shown. The genome sequencing of gene-complemented strains reveals the chromosomal integration of the plasmid with magnetosome genes at a specific site, most likely by catalysis of an endogenous transposase. Magnetosome production is successfully enhanced by integrating a variation of magnetosome gene operons in the chromosome. This chromosomal integration mechanism will allow the design of functional magnetosomes de novo and M. magneticum AMB-1 may be used as a chassis for the designed magnetosome production.},
doi = {10.1002/biot.202000278},
journal = {Biotechnology Journal},
number = 12,
volume = 15,
place = {United States},
year = {Wed Aug 26 00:00:00 EDT 2020},
month = {Wed Aug 26 00:00:00 EDT 2020}
}
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