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Title: Spectroradiometric detection of competitor diatoms and the grazer Poteriochromonas in algal cultures

Abstract

To address challenges in early detection of pond pests, we have extended a spectroradiometric monitoring method, initially demonstrated for measurement of pigment optical activity and biomass, to the detection of algal competitors and grazers. The method relies upon measurement and interpretation of pond reflectance spectra spanning from the visible into the near-infrared. Reflectance spectra are acquired every 5 min with a multi-channel, fiber-coupled spectroradiometer, providing monitoring of algal pond conditions with high temporal frequency. The spectra are interpreted via numerical inversion of a reflectance model, in which the abovewater reflectance is expressed in terms of the absorption and backscatter coefficients of the cultured species, with additional terms accounting for the pigment fluorescence features and for the water-surface reflection of sunlight and skylight. With this method we demonstrate detection of diatoms and the predator Poteriochromonas in outdoor cultures of Nannochloropsis oceanica and Chlorella vulgaris, respectively. Here, the relative strength of these signatures is compared to microscopy and sequencing analysis. Spectroradiometric detection of diatoms is then further assessed on beaker-contained mixtures of Microchloropsis salina with Phaeodactylum tricornutum, Thalassiosira weissflogii, and Thalassiosira pseudonana, respectively, providing an initial evaluation of the sensitivity and specificity of detecting pond competitors.

Authors:
 [1];  [2];  [3];  [4];  [5];  [1];  [1];  [1];  [2]
+ Show Author Affiliations
  1. Sandia National Laboratory (SNL-CA), Livermore, CA (United States)
  2. Sandia National Laboratory (SNL-NM), Albuquerque, NM (United States)
  3. Sandia National Laboratory (SNL-NM), Albuquerque, NM (United States); Agilent Technologies, Frederick, CO (United States)
  4. Arizona State University, Mesa, AZ (United States); Santa Fe Community College, NM (United States)
  5. Arizona State University, Mesa, AZ (United States)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States); Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; Algae Testbed Public Private Partnership
OSTI Identifier:
1828029
Alternate Identifier(s):
OSTI ID: 1772936; OSTI ID: 1785548; OSTI ID: 1809803
Report Number(s):
SAND-2021-3058J; SAND2021-3058J
Journal ID: ISSN 2211-9264; 694772
Grant/Contract Number:  
NA0003525; EE0005996; AC04-94AL85000; NL0032356
Resource Type:
Accepted Manuscript
Journal Name:
Algal Research
Additional Journal Information:
Journal Volume: 51; Journal ID: ISSN 2211-9264
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; algal crop protection; algal cultivation; reflectance spectroscopy

Citation Formats

Reichardt, Thomas A., Maes, Danae, Jensen, Travis J., Dempster, Thomas A., McGowen, John A., Poorey, Kunal, Curtis, Deanna J., Lane, Todd W., and Timlin, Jerilyn A. Spectroradiometric detection of competitor diatoms and the grazer Poteriochromonas in algal cultures. United States: N. p., 2020. Web. doi:10.1016/j.algal.2020.102020.
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Reichardt, Thomas A., Maes, Danae, Jensen, Travis J., Dempster, Thomas A., McGowen, John A., Poorey, Kunal, Curtis, Deanna J., Lane, Todd W., & Timlin, Jerilyn A. Spectroradiometric detection of competitor diatoms and the grazer Poteriochromonas in algal cultures. United States. https://doi.org/10.1016/j.algal.2020.102020
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Reichardt, Thomas A., Maes, Danae, Jensen, Travis J., Dempster, Thomas A., McGowen, John A., Poorey, Kunal, Curtis, Deanna J., Lane, Todd W., and Timlin, Jerilyn A. Mon . "Spectroradiometric detection of competitor diatoms and the grazer Poteriochromonas in algal cultures". United States. https://doi.org/10.1016/j.algal.2020.102020. https://www.osti.gov/servlets/purl/1828029.
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@article{osti_1828029,
title = {Spectroradiometric detection of competitor diatoms and the grazer Poteriochromonas in algal cultures},
author = {Reichardt, Thomas A. and Maes, Danae and Jensen, Travis J. and Dempster, Thomas A. and McGowen, John A. and Poorey, Kunal and Curtis, Deanna J. and Lane, Todd W. and Timlin, Jerilyn A.},
abstractNote = {To address challenges in early detection of pond pests, we have extended a spectroradiometric monitoring method, initially demonstrated for measurement of pigment optical activity and biomass, to the detection of algal competitors and grazers. The method relies upon measurement and interpretation of pond reflectance spectra spanning from the visible into the near-infrared. Reflectance spectra are acquired every 5 min with a multi-channel, fiber-coupled spectroradiometer, providing monitoring of algal pond conditions with high temporal frequency. The spectra are interpreted via numerical inversion of a reflectance model, in which the abovewater reflectance is expressed in terms of the absorption and backscatter coefficients of the cultured species, with additional terms accounting for the pigment fluorescence features and for the water-surface reflection of sunlight and skylight. With this method we demonstrate detection of diatoms and the predator Poteriochromonas in outdoor cultures of Nannochloropsis oceanica and Chlorella vulgaris, respectively. Here, the relative strength of these signatures is compared to microscopy and sequencing analysis. Spectroradiometric detection of diatoms is then further assessed on beaker-contained mixtures of Microchloropsis salina with Phaeodactylum tricornutum, Thalassiosira weissflogii, and Thalassiosira pseudonana, respectively, providing an initial evaluation of the sensitivity and specificity of detecting pond competitors.},
doi = {10.1016/j.algal.2020.102020},
journal = {Algal Research},
number = ,
volume = 51,
place = {United States},
year = {Mon Aug 03 00:00:00 EDT 2020},
month = {Mon Aug 03 00:00:00 EDT 2020}
}
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Journal Article:
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Publisher's Version of Record
https://doi.org/10.1016/j.algal.2020.102020
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