Imaging Supramolecular Morphogenesis with Confocal Laser Scanning Microscopy at Elevated Temperatures
Abstract
The morphogenesis of supramolecular assemblies is a highly dynamic process that has only recently been recognized, and our understanding of this phenomenon will require imaging techniques capable of crossing scales. Shape transformations depend both on the complex energy landscapes of supramolecular systems and the kinetically controlled pathways that define their structures and functions. We report here the use of confocal laser scanning microscopy coupled with a custom-designed variable-temperature sample stage that enables in situ observation of such shape changes. The submicrometer resolution of this technique allows for real-time observation of the nanostructures in the native liquid environments in which they transform with thermal energy. We use this technique to study the temperature-dependent morphogenic behavior of peptide amphiphile nanofibers and photocatalytic chromophore amphiphile nanoribbons. The variable-temperature confocal microscopy technique demonstrated in this work can sample a large volume and provides real-time information on thermally induced morphological changes in the solution.
- Authors:
-
[1];
[2];
[3];
[4]
- Northwestern Univ., Chicago, IL (United States)
- Northwestern Univ., Evanston, IL (United States)
- Northwestern Univ., Chicago, IL (United States); Northwestern Univ., Evanston, IL (United States)
- Northwestern Univ., Evanston, IL (United States); Northwestern Univ., Chicago, IL (United States)
- Publication Date:
- Research Org.:
- Energy Frontier Research Centers (EFRC) (United States). Center for Bio-Inspired Energy Science (CBES)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1822879
- Alternate Identifier(s):
- OSTI ID: 1846637
- Grant/Contract Number:
- SC0000989
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nano Letters
- Additional Journal Information:
- Journal Volume: 20; Journal Issue: 6; Journal ID: ISSN 1530-6984
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 77 NANOSCIENCE AND NANOTECHNOLOGY; 99 GENERAL AND MISCELLANEOUS; Supramolecular structures and assemblies; Nanofibers; Crystallization; Amphiphiles; Annealing (metallurgy); Supramolecular assembly; Confocal microscopy; In situ microscopy; Nanoribbons; 36 MATERIALS SCIENCE
Citation Formats
Sai, Hiroaki, Lau, Garrett C., Dannenhoffer, Adam J., Chin, Stacey M., D̵ord̵ević, Luka, and Stupp, Samuel I. Imaging Supramolecular Morphogenesis with Confocal Laser Scanning Microscopy at Elevated Temperatures. United States: N. p., 2020.
Web. doi:10.1021/acs.nanolett.0c00662.
Sai, Hiroaki, Lau, Garrett C., Dannenhoffer, Adam J., Chin, Stacey M., D̵ord̵ević, Luka, & Stupp, Samuel I. Imaging Supramolecular Morphogenesis with Confocal Laser Scanning Microscopy at Elevated Temperatures. United States. https://doi.org/10.1021/acs.nanolett.0c00662
Sai, Hiroaki, Lau, Garrett C., Dannenhoffer, Adam J., Chin, Stacey M., D̵ord̵ević, Luka, and Stupp, Samuel I. Fri .
"Imaging Supramolecular Morphogenesis with Confocal Laser Scanning Microscopy at Elevated Temperatures". United States. https://doi.org/10.1021/acs.nanolett.0c00662. https://www.osti.gov/servlets/purl/1822879.
@article{osti_1822879,
title = {Imaging Supramolecular Morphogenesis with Confocal Laser Scanning Microscopy at Elevated Temperatures},
author = {Sai, Hiroaki and Lau, Garrett C. and Dannenhoffer, Adam J. and Chin, Stacey M. and D̵ord̵ević, Luka and Stupp, Samuel I.},
abstractNote = {The morphogenesis of supramolecular assemblies is a highly dynamic process that has only recently been recognized, and our understanding of this phenomenon will require imaging techniques capable of crossing scales. Shape transformations depend both on the complex energy landscapes of supramolecular systems and the kinetically controlled pathways that define their structures and functions. We report here the use of confocal laser scanning microscopy coupled with a custom-designed variable-temperature sample stage that enables in situ observation of such shape changes. The submicrometer resolution of this technique allows for real-time observation of the nanostructures in the native liquid environments in which they transform with thermal energy. We use this technique to study the temperature-dependent morphogenic behavior of peptide amphiphile nanofibers and photocatalytic chromophore amphiphile nanoribbons. The variable-temperature confocal microscopy technique demonstrated in this work can sample a large volume and provides real-time information on thermally induced morphological changes in the solution.},
doi = {10.1021/acs.nanolett.0c00662},
journal = {Nano Letters},
number = 6,
volume = 20,
place = {United States},
year = {Fri May 08 00:00:00 EDT 2020},
month = {Fri May 08 00:00:00 EDT 2020}
}
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