Mimicking the Bioactivity of Fibroblast Growth Factor-2 Using Supramolecular Nanoribbons
Abstract
Fibroblast growth factor (FGF-2) is a multifunctional growth factor that has pleiotropic effects in different tissues and organs. In particular, FGF-2 has a special role in angiogenesis, an important process in development, wound healing, cell survival, and differentiation. Therefore, incorporating biological agents like FGF-2 within therapeutic biomaterials is a potential strategy to create angiogenic bioactivity for the repair of damaged tissue caused by trauma or complications that arise from age and/or disease. However, the use of growth factors as therapeutic agents can be costly and does not always bring about efficient tissue repair due to rapid clearance from the targeted site. An alternative would be a stable supramolecular nanostructure with the capacity to activate the FGF-2 receptor that can also assemble into a scaffold deliverable to tissue. We report here on peptide amphiphiles that incorporate a peptide known to activate the FGF-2 receptor and peptide domains that drive its self-assembly into supramolecular nanoribbons. These FGF2-PA nanoribbons displayed the ability to increase the proliferation and migration of the human umbilical vein endothelial cells (HUVECs) in vitro to the same extent as the native FGF-2 protein at certain concentrations. We confirmed that this activity was specific to the FGFR1 signaling pathway bymore »
- Authors:
-
[3]
- Northwestern Univ., Chicago, IL (United States)
- Northwestern Univ., Evanston, IL (United States)
- Northwestern Univ., Chicago, IL (United States); Northwestern Univ., Evanston, IL (United States)
- Publication Date:
- Research Org.:
- Northwestern Univ., Evanston, IL (United States); Energy Frontier Research Centers (EFRC) (United States). Center for Bio-Inspired Energy Science (CBES)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1821894
- Alternate Identifier(s):
- OSTI ID: 1846790
- Grant/Contract Number:
- FG02-00ER45810
- Resource Type:
- Accepted Manuscript
- Journal Name:
- ACS Biomaterials Science & Engineering
- Additional Journal Information:
- Journal Volume: 3; Journal Issue: 9; Journal ID: ISSN 2373-9878
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 99 GENERAL AND MISCELLANEOUS; supramolecular biomaterials; peptide amphiphiles; nanoribbons; fibroblast growth factor-2; mimetic peptide; nanostructures; cell signaling; peptides and proteins; two dimensional materials; bioactivity; Nanostructures, Cell signaling, Peptides and proteins, Two dimensional materials, Bioactivity
Citation Formats
Rubert Pérez, Charles M., Álvarez, Zaida, Chen, Feng, Aytun, Taner, and Stupp, Samuel I. Mimicking the Bioactivity of Fibroblast Growth Factor-2 Using Supramolecular Nanoribbons. United States: N. p., 2017.
Web. doi:10.1021/acsbiomaterials.7b00347.
Rubert Pérez, Charles M., Álvarez, Zaida, Chen, Feng, Aytun, Taner, & Stupp, Samuel I. Mimicking the Bioactivity of Fibroblast Growth Factor-2 Using Supramolecular Nanoribbons. United States. https://doi.org/10.1021/acsbiomaterials.7b00347
Rubert Pérez, Charles M., Álvarez, Zaida, Chen, Feng, Aytun, Taner, and Stupp, Samuel I. Sun .
"Mimicking the Bioactivity of Fibroblast Growth Factor-2 Using Supramolecular Nanoribbons". United States. https://doi.org/10.1021/acsbiomaterials.7b00347. https://www.osti.gov/servlets/purl/1821894.
@article{osti_1821894,
title = {Mimicking the Bioactivity of Fibroblast Growth Factor-2 Using Supramolecular Nanoribbons},
author = {Rubert Pérez, Charles M. and Álvarez, Zaida and Chen, Feng and Aytun, Taner and Stupp, Samuel I.},
abstractNote = {Fibroblast growth factor (FGF-2) is a multifunctional growth factor that has pleiotropic effects in different tissues and organs. In particular, FGF-2 has a special role in angiogenesis, an important process in development, wound healing, cell survival, and differentiation. Therefore, incorporating biological agents like FGF-2 within therapeutic biomaterials is a potential strategy to create angiogenic bioactivity for the repair of damaged tissue caused by trauma or complications that arise from age and/or disease. However, the use of growth factors as therapeutic agents can be costly and does not always bring about efficient tissue repair due to rapid clearance from the targeted site. An alternative would be a stable supramolecular nanostructure with the capacity to activate the FGF-2 receptor that can also assemble into a scaffold deliverable to tissue. We report here on peptide amphiphiles that incorporate a peptide known to activate the FGF-2 receptor and peptide domains that drive its self-assembly into supramolecular nanoribbons. These FGF2-PA nanoribbons displayed the ability to increase the proliferation and migration of the human umbilical vein endothelial cells (HUVECs) in vitro to the same extent as the native FGF-2 protein at certain concentrations. We confirmed that this activity was specific to the FGFR1 signaling pathway by tracking the phosphorylation of downstream signaling effectors such ERK1/2 and pH3. These results indicated the specificity of FGF2-PA nanoribbons in activating the FGF-2 signaling pathway and its potential application as a supramolecular scaffold that can be used in vivo as an alternative to the encapsulation and delivery of the native FGF-2 protein.},
doi = {10.1021/acsbiomaterials.7b00347},
journal = {ACS Biomaterials Science & Engineering},
number = 9,
volume = 3,
place = {United States},
year = {Sun Aug 06 00:00:00 EDT 2017},
month = {Sun Aug 06 00:00:00 EDT 2017}
}
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