Pathway discovery and engineering for cleavage of a β-1 lignin-derived biaryl compound
Abstract
Lignin biosynthesis typically results in a polymer with several inter-monomer bond linkages, and the heterogeneity of linkages presents a challenge for depolymerization processes. While several enzyme classes have been shown to cleave common dimer linkages in lignin, the pathway of bacterial ß-1 spirodienone linkage cleavage has not been elucidated. Here, we identified a pathway for cleavage of 1,2-diguaiacylpropane-1,3-diol (DGPD), a ß-1 linked biaryl representative of a ring-opened spirodienone linkage, in Novosphingobium aromaticivorans DSM12444. In vitro assays using cell lysates demonstrated that RS14230 (LsdE) converts DGPD to a lignostilbene intermediate, which the carotenoid oxygenase, LsdA, then converts to vanillin. A Pseudomonas putida KT2440 strain engineered with lsdEA expression catabolizes erythro-DGPD, but not threo-DGPD. We further engineered P. putida to convert DGPD to a product, cis,cis-muconic acid. Overall, this work demonstrates the potential to identify new enzymatic reactions in N. aromaticivorans and expands the biological funnel of P. putida for microbial lignin valorization.
- Authors:
-
[2];
[4];
[3];
[1];
[1]
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL); National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; National Science Foundation (NSF); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office
- OSTI Identifier:
- 1771901
- Alternate Identifier(s):
- OSTI ID: 1772445; OSTI ID: 1815315
- Report Number(s):
- NREL/JA-2A00-78148
Journal ID: ISSN 1096-7176
- Grant/Contract Number:
- AC05-00OR22725; AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Metabolic Engineering
- Additional Journal Information:
- Journal Volume: 65; Journal ID: ISSN 1096-7176
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; lignin valorization; dimer catabolism; novosphingobium aromaticivorans DSM12444; pseudomonas putida KT2440
Citation Formats
Presley, Gerald N., Werner, Allison Z., Katahira, Rui, Garcia, David C., Haugen, Stefan J., Ramirez, Kelsey J., Giannone, Richard J., Beckham, Gregg T., and Michener, Joshua K. Pathway discovery and engineering for cleavage of a β-1 lignin-derived biaryl compound. United States: N. p., 2021.
Web. doi:10.1016/j.ymben.2021.02.003.
Presley, Gerald N., Werner, Allison Z., Katahira, Rui, Garcia, David C., Haugen, Stefan J., Ramirez, Kelsey J., Giannone, Richard J., Beckham, Gregg T., & Michener, Joshua K. Pathway discovery and engineering for cleavage of a β-1 lignin-derived biaryl compound. United States. https://doi.org/10.1016/j.ymben.2021.02.003
Presley, Gerald N., Werner, Allison Z., Katahira, Rui, Garcia, David C., Haugen, Stefan J., Ramirez, Kelsey J., Giannone, Richard J., Beckham, Gregg T., and Michener, Joshua K. Tue .
"Pathway discovery and engineering for cleavage of a β-1 lignin-derived biaryl compound". United States. https://doi.org/10.1016/j.ymben.2021.02.003. https://www.osti.gov/servlets/purl/1771901.
@article{osti_1771901,
title = {Pathway discovery and engineering for cleavage of a β-1 lignin-derived biaryl compound},
author = {Presley, Gerald N. and Werner, Allison Z. and Katahira, Rui and Garcia, David C. and Haugen, Stefan J. and Ramirez, Kelsey J. and Giannone, Richard J. and Beckham, Gregg T. and Michener, Joshua K.},
abstractNote = {Lignin biosynthesis typically results in a polymer with several inter-monomer bond linkages, and the heterogeneity of linkages presents a challenge for depolymerization processes. While several enzyme classes have been shown to cleave common dimer linkages in lignin, the pathway of bacterial ß-1 spirodienone linkage cleavage has not been elucidated. Here, we identified a pathway for cleavage of 1,2-diguaiacylpropane-1,3-diol (DGPD), a ß-1 linked biaryl representative of a ring-opened spirodienone linkage, in Novosphingobium aromaticivorans DSM12444. In vitro assays using cell lysates demonstrated that RS14230 (LsdE) converts DGPD to a lignostilbene intermediate, which the carotenoid oxygenase, LsdA, then converts to vanillin. A Pseudomonas putida KT2440 strain engineered with lsdEA expression catabolizes erythro-DGPD, but not threo-DGPD. We further engineered P. putida to convert DGPD to a product, cis,cis-muconic acid. Overall, this work demonstrates the potential to identify new enzymatic reactions in N. aromaticivorans and expands the biological funnel of P. putida for microbial lignin valorization.},
doi = {10.1016/j.ymben.2021.02.003},
journal = {Metabolic Engineering},
number = ,
volume = 65,
place = {United States},
year = {Tue Feb 23 00:00:00 EST 2021},
month = {Tue Feb 23 00:00:00 EST 2021}
}
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