The importance of nutrients for microbial priming in a bog rhizosphere
Abstract
Wetlands host anaerobic microbes which convert organic carbon into methane (CH4), a powerful greenhouse gas. Wetland plants can influence which carbon compounds are available for microbial processing by exuding freshly fixed carbon from their roots. Notably, exudation of carbon from plant roots can trigger microbial priming: the process of new carbon stimulating the microbial community into processing more soil carbon than they otherwise would have. This study utilized high resolution Fourier transform ion cyclotron mass spectrometry (FT-ICR-MS) analysis to probe the chemical composition of soil organic compounds from the rhizosphere of Carex aquatillis, a common wetland sedge, which is known to have stimulated microbial priming within a peat soil. The goal was to identify what types of molecules are created or lost during microbial priming in the wetland rhizosphere and thus advance mechanistic understanding of the process. FT-ICR-MS analysis demonstrated that more microbial transformations of carbon occurred among water-soluble compounds than among hydrophobic compounds, but that some hydrophobic compounds were processed as well. Crucially for understanding microbial priming, the root exudates triggered increased processing of high molecular weight molecules regardless of nutrient content but processed low molecular weight compounds only if they contained essential nutrients. Furthermore, the pattern of whichmore »
- Authors:
-
[1];
[2];
[3];
[1]
- Univ. of Washington, Seattle, WA (United States)
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL); Univ. of Arizona, Tucson, AZ (United States)
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Workforce Development for Teachers and Scientists (WDTS)
- OSTI Identifier:
- 1767053
- Report Number(s):
- PNNL-SA-159934
Journal ID: ISSN 0168-2563
- Grant/Contract Number:
- AC05-76RL01830; SC0010338; SC0014664
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biogeochemistry
- Additional Journal Information:
- Journal Volume: 152; Journal Issue: 2-3; Journal ID: ISSN 0168-2563
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; methane; wetlands; microbial priming; root exudates; climate change
Citation Formats
Waldo, Nicholas B., Tfaily, Malak M., Anderton, Christopher, and Neumann, Rebecca B. The importance of nutrients for microbial priming in a bog rhizosphere. United States: N. p., 2021.
Web. doi:10.1007/s10533-021-00754-2.
Waldo, Nicholas B., Tfaily, Malak M., Anderton, Christopher, & Neumann, Rebecca B. The importance of nutrients for microbial priming in a bog rhizosphere. United States. https://doi.org/10.1007/s10533-021-00754-2
Waldo, Nicholas B., Tfaily, Malak M., Anderton, Christopher, and Neumann, Rebecca B. Tue .
"The importance of nutrients for microbial priming in a bog rhizosphere". United States. https://doi.org/10.1007/s10533-021-00754-2. https://www.osti.gov/servlets/purl/1767053.
@article{osti_1767053,
title = {The importance of nutrients for microbial priming in a bog rhizosphere},
author = {Waldo, Nicholas B. and Tfaily, Malak M. and Anderton, Christopher and Neumann, Rebecca B.},
abstractNote = {Wetlands host anaerobic microbes which convert organic carbon into methane (CH4), a powerful greenhouse gas. Wetland plants can influence which carbon compounds are available for microbial processing by exuding freshly fixed carbon from their roots. Notably, exudation of carbon from plant roots can trigger microbial priming: the process of new carbon stimulating the microbial community into processing more soil carbon than they otherwise would have. This study utilized high resolution Fourier transform ion cyclotron mass spectrometry (FT-ICR-MS) analysis to probe the chemical composition of soil organic compounds from the rhizosphere of Carex aquatillis, a common wetland sedge, which is known to have stimulated microbial priming within a peat soil. The goal was to identify what types of molecules are created or lost during microbial priming in the wetland rhizosphere and thus advance mechanistic understanding of the process. FT-ICR-MS analysis demonstrated that more microbial transformations of carbon occurred among water-soluble compounds than among hydrophobic compounds, but that some hydrophobic compounds were processed as well. Crucially for understanding microbial priming, the root exudates triggered increased processing of high molecular weight molecules regardless of nutrient content but processed low molecular weight compounds only if they contained essential nutrients. Furthermore, the pattern of which molecules are processed and which are not is evidence for a selective priming effect in which some types of carbon compounds are used at an increased rate, while others are not.},
doi = {10.1007/s10533-021-00754-2},
journal = {Biogeochemistry},
number = 2-3,
volume = 152,
place = {United States},
year = {Tue Jan 26 00:00:00 EST 2021},
month = {Tue Jan 26 00:00:00 EST 2021}
}
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