Wireworm (Coleoptera: Elateridae) genomic analysis reveals putative cryptic species, population structure, and adaptation to pest control
Abstract
The larvae of click beetles (Coleoptera: Elateridae), known as “wireworms,” are agricultural pests that pose a substantial economic threat worldwide. We produced one of the first wireworm genome assemblies (Limonius californicus), and investigated population structure and phylogenetic relationships of three species (L. californicus, L. infuscatus, L. canus) across the northwest US and southwest Canada using genome-wide markers (RADseq) and genome skimming. We found two species (L. californicus and L. infuscatus) are comprised of multiple genetically distinct groups that diverged in the Pleistocene but have no known distinguishing morphological characters, and therefore could be considered cryptic species complexes. We also found within-species population structure across relatively short geographic distances. Genome scans for selection provided preliminary evidence for signatures of adaptation associated with different pesticide treatments in an agricultural field trial for L. canus. We demonstrate that genomic tools can be a strong asset in developing effective wireworm control strategies.
- Authors:
-
[1];
[1];
[4];
[1]
- Univ. of Idaho, Moscow, ID (United States)
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Washington State Univ., Pullman, WA (United States)
- Oregon State Univ., Hermiston, OR (United States)
- Agriculture and Agri-Food Canada, Agassiz, BC (Canada)
- Sentinel IPM Services, Chilliwack, BC (Canada)
- Montana State Univ., Bozeman, MT (United States)
- Idaho Wheat Commission, Boise, ID (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1665864
- Report Number(s):
- NREL/JA-2C00-77923
Journal ID: ISSN 2399-3642; MainId:31832;UUID:cd1cd1d9-6882-43dd-be33-4a4d386abcfd;MainAdminID:18536
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Communications Biology
- Additional Journal Information:
- Journal Volume: 3; Journal Issue: 1; Journal ID: ISSN 2399-3642
- Publisher:
- Springer Nature
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biogeography; molecular ecology; phylogenetics; population genetics
Citation Formats
Andrews, Kimberly R., Gerritsen, Alida, Rashed, Arash, Crowder, David W., Rondon, Silvia I., van Herk, Willem G., Vernon, Robert, Wanner, Kevin W., Wilson, Cathy M., New, Daniel D., Fagnan, Matthew W., Hohenlohe, Paul A., and Hunter, Samuel S. Wireworm (Coleoptera: Elateridae) genomic analysis reveals putative cryptic species, population structure, and adaptation to pest control. United States: N. p., 2020.
Web. doi:10.1038/s42003-020-01169-9.
Andrews, Kimberly R., Gerritsen, Alida, Rashed, Arash, Crowder, David W., Rondon, Silvia I., van Herk, Willem G., Vernon, Robert, Wanner, Kevin W., Wilson, Cathy M., New, Daniel D., Fagnan, Matthew W., Hohenlohe, Paul A., & Hunter, Samuel S. Wireworm (Coleoptera: Elateridae) genomic analysis reveals putative cryptic species, population structure, and adaptation to pest control. United States. https://doi.org/10.1038/s42003-020-01169-9
Andrews, Kimberly R., Gerritsen, Alida, Rashed, Arash, Crowder, David W., Rondon, Silvia I., van Herk, Willem G., Vernon, Robert, Wanner, Kevin W., Wilson, Cathy M., New, Daniel D., Fagnan, Matthew W., Hohenlohe, Paul A., and Hunter, Samuel S. Mon .
"Wireworm (Coleoptera: Elateridae) genomic analysis reveals putative cryptic species, population structure, and adaptation to pest control". United States. https://doi.org/10.1038/s42003-020-01169-9. https://www.osti.gov/servlets/purl/1665864.
@article{osti_1665864,
title = {Wireworm (Coleoptera: Elateridae) genomic analysis reveals putative cryptic species, population structure, and adaptation to pest control},
author = {Andrews, Kimberly R. and Gerritsen, Alida and Rashed, Arash and Crowder, David W. and Rondon, Silvia I. and van Herk, Willem G. and Vernon, Robert and Wanner, Kevin W. and Wilson, Cathy M. and New, Daniel D. and Fagnan, Matthew W. and Hohenlohe, Paul A. and Hunter, Samuel S.},
abstractNote = {The larvae of click beetles (Coleoptera: Elateridae), known as “wireworms,” are agricultural pests that pose a substantial economic threat worldwide. We produced one of the first wireworm genome assemblies (Limonius californicus), and investigated population structure and phylogenetic relationships of three species (L. californicus, L. infuscatus, L. canus) across the northwest US and southwest Canada using genome-wide markers (RADseq) and genome skimming. We found two species (L. californicus and L. infuscatus) are comprised of multiple genetically distinct groups that diverged in the Pleistocene but have no known distinguishing morphological characters, and therefore could be considered cryptic species complexes. We also found within-species population structure across relatively short geographic distances. Genome scans for selection provided preliminary evidence for signatures of adaptation associated with different pesticide treatments in an agricultural field trial for L. canus. We demonstrate that genomic tools can be a strong asset in developing effective wireworm control strategies.},
doi = {10.1038/s42003-020-01169-9},
journal = {Communications Biology},
number = 1,
volume = 3,
place = {United States},
year = {Mon Sep 07 00:00:00 EDT 2020},
month = {Mon Sep 07 00:00:00 EDT 2020}
}
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Fig. 1: Map of sampling locations color-coded by genetically distinct groups identified using PCA and sNMF. a L. canus, colored black; and L. californicus, with group names (A–D) following the circles in Fig. 4a. b L. infuscatus, with group names (E–G) following the circles in Fig. 4b. Samples from bothmore »
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