Cell-free gene expression: an expanded repertoire of applications
Abstract
Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-yielding (grams of protein per litre), cell-free gene expression systems from model bacteria, the development of cell-free platforms from non-model organisms and multiplexed strategies for rapidly assessing biological design. These advances provide exciting opportunities to profoundly transform synthetic biology by enabling new approaches to the model-driven design of synthetic gene networks, the fast and portable sensing of compounds, on-demand biomanufacturing, building cells from the bottom up, and next-generation educational kits.
- Authors:
-
- Northwestern Univ., Evanston, IL (United States). Chemistry of Life Processes Institute and Center for Synthetic Biology
- Northwestern Univ., Evanston, IL (United States); Northwestern Univ., Chicago, IL (United States). Center for Synthetic Biology, Robert H. Lurie Comprehensive Cancer Center, and Simpson Querrey Institute
- Publication Date:
- Research Org.:
- Northwestern Univ., Evanston, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1660454
- Alternate Identifier(s):
- OSTI ID: 2301593
- Grant/Contract Number:
- SC0018249; EE0008354; EE0008343
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Reviews. Genetics
- Additional Journal Information:
- Journal Volume: 21; Journal Issue: 3; Journal ID: ISSN 1471-0056
- Publisher:
- Springer Nature
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Silverman, Adam D., Karim, Ashty S., and Jewett, Michael C. Cell-free gene expression: an expanded repertoire of applications. United States: N. p., 2019.
Web. doi:10.1038/s41576-019-0186-3.
Silverman, Adam D., Karim, Ashty S., & Jewett, Michael C. Cell-free gene expression: an expanded repertoire of applications. United States. https://doi.org/10.1038/s41576-019-0186-3
Silverman, Adam D., Karim, Ashty S., and Jewett, Michael C. Thu .
"Cell-free gene expression: an expanded repertoire of applications". United States. https://doi.org/10.1038/s41576-019-0186-3. https://www.osti.gov/servlets/purl/1660454.
@article{osti_1660454,
title = {Cell-free gene expression: an expanded repertoire of applications},
author = {Silverman, Adam D. and Karim, Ashty S. and Jewett, Michael C.},
abstractNote = {Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-yielding (grams of protein per litre), cell-free gene expression systems from model bacteria, the development of cell-free platforms from non-model organisms and multiplexed strategies for rapidly assessing biological design. These advances provide exciting opportunities to profoundly transform synthetic biology by enabling new approaches to the model-driven design of synthetic gene networks, the fast and portable sensing of compounds, on-demand biomanufacturing, building cells from the bottom up, and next-generation educational kits.},
doi = {10.1038/s41576-019-0186-3},
journal = {Nature Reviews. Genetics},
number = 3,
volume = 21,
place = {United States},
year = {Thu Nov 28 00:00:00 EST 2019},
month = {Thu Nov 28 00:00:00 EST 2019}
}
Web of Science
Works referenced in this record:
Expression optimization and synthetic gene networks in cell-free systems
journal, December 2011
- Karig, David K.; Iyer, Sukanya; Simpson, Michael L.
- Nucleic Acids Research, Vol. 40, Issue 8
Cell-free Protein Synthesis from a Release Factor 1 Deficient Escherichia coli Activates Efficient and Multiple Site-specific Nonstandard Amino Acid Incorporation
journal, January 2014
- Hong, Seok Hoon; Ntai, Ioanna; Haimovich, Adrian D.
- ACS Synthetic Biology, Vol. 3, Issue 6
Establishing a Cell-Free Vibrio natriegens Expression System
journal, August 2018
- Wiegand, Daniel J.; Lee, Henry H.; Ostrov, Nili
- ACS Synthetic Biology, Vol. 7, Issue 10
Single step generation of protein arrays from DNA by cell-free expression and in situ immobilisation (PISA method)
journal, August 2001
- He, M.
- Nucleic Acids Research, Vol. 29, Issue 15
Highly sensitive detection of gene expression of an intronless gene: amplification of mRNA, but not genomic DNA by nucleic acid sequence based amplification (NASBA)
journal, May 1998
- Heim, A.
- Nucleic Acids Research, Vol. 26, Issue 9
Cell-Free and In Vivo Characterization of Lux, Las, and Rpa Quorum Activation Systems in E. coli
journal, November 2017
- Halleran, Andrew D.; Murray, Richard M.
- ACS Synthetic Biology, Vol. 7, Issue 2
Rapid and Scalable Preparation of Bacterial Lysates for Cell-Free Gene Expression
journal, August 2017
- Didovyk, Andriy; Tonooka, Taishi; Tsimring, Lev
- ACS Synthetic Biology, Vol. 6, Issue 12
Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines
journal, March 2011
- Zawada, James F.; Yin, Gang; Steiner, Alexander R.
- Biotechnology and Bioengineering, Vol. 108, Issue 7
Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements
journal, November 2016
- Kelwick, Richard; Webb, Alexander J.; MacDonald, James T.
- Metabolic Engineering, Vol. 38
A novel cell-free translation/glycosylation system prepared from insect cells
journal, January 2000
- Tarui, Hiroshi; Imanishi, Shigeo; Hara, Toshio
- Journal of Bioscience and Bioengineering, Vol. 90, Issue 5
Oligonucleotide-mediated tRNA sequestration enables one-pot sense codon reassignment in vitro
journal, May 2018
- Cui, Zhenling; Wu, Yue; Mureev, Sergey
- Nucleic Acids Research, Vol. 46, Issue 12
Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system
journal, March 2012
- Yin, Gang; Garces, Eudean D.; Yang, Junhao
- mAbs, Vol. 4, Issue 2
Quantitative polysome analysis identifies limitations in bacterial cell-free protein synthesis
journal, January 2005
- Underwood, Kelly A.; Swartz, James R.; Puglisi, Joseph D.
- Biotechnology and Bioengineering, Vol. 91, Issue 4
Towards synthesis of a minimal cell
journal, January 2006
- Forster, Anthony C.; Church, George M.
- Molecular Systems Biology, Vol. 2, Issue 1
PERSIA for Direct Fluorescence Measurements of Transcription, Translation, and Enzyme Activity in Cell-Free Systems
journal, March 2019
- Wick, Scott; Walsh, David I.; Bobrow, Johanna
- ACS Synthetic Biology, Vol. 8, Issue 5
Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis
journal, July 2016
- Dudley, Quentin M.; Anderson, Kim C.; Jewett, Michael C.
- ACS Synthetic Biology, Vol. 5, Issue 12
Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases
journal, May 2018
- Kightlinger, Weston; Lin, Liang; Rosztoczy, Madisen
- Nature Chemical Biology, Vol. 14, Issue 6
Quantitative Characterization of Translational Riboregulators Using an in Vitro Transcription–Translation System
journal, April 2018
- Senoussi, Anis; Lee Tin Wah, Jonathan; Shimizu, Yoshihiro
- ACS Synthetic Biology, Vol. 7, Issue 5
Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids
journal, March 2018
- Martin, Rey W.; Des Soye, Benjamin J.; Kwon, Yong-Chan
- Nature Communications, Vol. 9, Issue 1
Rapid acquisition and model-based analysis of cell-free transcription–translation reactions from nonmodel bacteria
journal, April 2018
- Moore, Simon J.; MacDonald, James T.; Wienecke, Sarah
- Proceedings of the National Academy of Sciences, Vol. 115, Issue 19
Rapid recombinant protein expression in cell-free extracts from human blood
journal, June 2018
- Burgenson, David; Gurramkonda, Chandrasekhar; Pilli, Manohar
- Scientific Reports, Vol. 8, Issue 1
Coarse-Grained Dynamics of Protein Synthesis in a Cell-Free System
journal, January 2011
- Karzbrun, Eyal; Shin, Jonghyeon; Bar-Ziv, Roy H.
- Physical Review Letters, Vol. 106, Issue 4
Prolonging Cell-Free Protein Synthesis by Selective Reagent Additions
journal, June 2000
- Kim, D. -M.; Swartz, J. R.
- Biotechnology Progress, Vol. 16, Issue 3
A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases
journal, December 2017
- Schoborg, Jennifer A.; Hershewe, Jasmine M.; Stark, Jessica C.
- Biotechnology and Bioengineering, Vol. 115, Issue 3
Prolonging cell-free protein synthesis with a novel ATP regeneration system
journal, January 1999
- Kim, Dong-Myung; Swartz, James R.
- Biotechnology and Bioengineering, Vol. 66, Issue 3
Efficient production of isotopically labeled proteins by cell-free synthesis: A practical protocol
journal, November 2004
- Torizawa, Takuya; Shimizu, Masato; Taoka, Masato
- Journal of Biomolecular NMR, Vol. 30, Issue 3
Implementation of cell-free biological networks at steady state
journal, September 2013
- Niederholtmeyer, H.; Stepanova, V.; Maerkl, S. J.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 40
Engineering Transcriptional Regulator Effector Specificity Using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor
journal, August 2015
- de los Santos, Emmanuel L. C.; Meyerowitz, Joseph T.; Mayo, Stephen L.
- ACS Synthetic Biology, Vol. 5, Issue 4
Cell-free protein synthesis: Applications come of age
journal, September 2012
- Carlson, Erik D.; Gan, Rui; Hodgman, C. Eric
- Biotechnology Advances, Vol. 30, Issue 5
A Semicontinuous Prokaryotic Coupled Transcription/Translation System Using a Dialysis Membrane
journal, October 1996
- Kim, D. -M.; Choi, C. -Y.
- Biotechnology Progress, Vol. 12, Issue 5
Artificial photosynthetic cell producing energy for protein synthesis
journal, March 2019
- Berhanu, Samuel; Ueda, Takuya; Kuruma, Yutetsu
- Nature Communications, Vol. 10, Issue 1
A paper-based, cell-free biosensor system for the detection of heavy metals and date rape drugs
journal, March 2019
- Gräwe, Alexander; Dreyer, Anna; Vornholt, Tobias
- PLOS ONE, Vol. 14, Issue 3
Cell-free translation reconstituted with purified components
journal, August 2001
- Shimizu, Yoshihiro; Inoue, Akio; Tomari, Yukihide
- Nature Biotechnology, Vol. 19, Issue 8
Field-deployable viral diagnostics using CRISPR-Cas13
journal, April 2018
- Myhrvold, Cameron; Freije, Catherine A.; Gootenberg, Jonathan S.
- Science, Vol. 360, Issue 6387
A theoretical study on the amount of ATP required for synthesis of microbial cell material
journal, December 1973
- Stouthamer, A. H.
- Antonie van Leeuwenhoek, Vol. 39, Issue 1
Efficient production of a bioactive, multiple disulfide-bonded protein using modified extracts ofEscherichia coli
journal, January 2003
- Kim, Dong-Myung; Swartz, James R.
- Biotechnology and Bioengineering, Vol. 85, Issue 2
Streamlined extract preparation for Escherichia coli -based cell-free protein synthesis by sonication or bead vortex mixing
journal, September 2012
- Shrestha, Prashanta; Holland, Troy Michael; Bundy, Bradley Charles
- BioTechniques, Vol. 53, Issue 3
Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System
journal, January 2014
- Zimmerman, Erik S.; Heibeck, Tyler H.; Gill, Avinash
- Bioconjugate Chemistry, Vol. 25, Issue 2
Experimentally Validated Model Enables Debottlenecking of in Vitro Protein Synthesis and Identifies a Control Shift under in Vivo Conditions
journal, June 2017
- Nieß, Alexander; Failmezger, Jurek; Kuschel, Maike
- ACS Synthetic Biology, Vol. 6, Issue 10
Development of a CHO-Based Cell-Free Platform for Synthesis of Active Monoclonal Antibodies
journal, April 2017
- Martin, Rey W.; Majewska, Natalia I.; Chen, Cindy X.
- ACS Synthetic Biology, Vol. 6, Issue 7
Rapidly Characterizing the Fast Dynamics of RNA Genetic Circuitry with Cell-Free Transcription–Translation (TX-TL) Systems
journal, March 2014
- Takahashi, Melissa K.; Chappell, James; Hayes, Clarmyra A.
- ACS Synthetic Biology, Vol. 4, Issue 5
Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array
journal, August 2019
- Zhang, Yi; Minagawa, Yoshihiro; Kizoe, Hiroto
- Science Advances, Vol. 5, Issue 8
Detergent proteases
journal, August 2004
- Maurer, K.
- Current Opinion in Biotechnology, Vol. 15, Issue 4
Barcoding Biological Reactions with DNA‐Functionalized Vesicles
journal, October 2019
- Peruzzi, Justin A.; Jacobs, Miranda L.; Vu, Timothy Q.
- Angewandte Chemie, Vol. 131, Issue 51
BioBits Health: Classroom Activities Exploring Engineering, Biology, and Human Health with Fluorescent Readouts
journal, March 2019
- Stark, Jessica C.; Huang, Ally; Hsu, Karen J.
- ACS Synthetic Biology, Vol. 8, Issue 5
A cell-free expression and purification process for rapid production of protein biologics
journal, December 2015
- Sullivan, Challise J.; Pendleton, Erik D.; Sasmor, Henri H.
- Biotechnology Journal, Vol. 11, Issue 2
Optimization of a blueprint for in vitro glycolysis by metabolic real-time analysis
journal, March 2011
- Bujara, Matthias; Schümperli, Michael; Pellaux, René
- Nature Chemical Biology, Vol. 7, Issue 5
Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate
journal, July 2013
- Sokolova, E.; Spruijt, E.; Hansen, M. M. K.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 29
Point-of-care production of therapeutic proteins of good-manufacturing-practice quality
journal, July 2018
- Adiga, Rajani; Al-adhami, Mustafa; Andar, Abhay
- Nature Biomedical Engineering, Vol. 2, Issue 9
Cell-free synthesis of a functional G protein-coupled receptor complexed with nanometer scale bilayer discs
journal, January 2011
- Yang, Jian-Ping; Cirico, Tatiana; Katzen, Federico
- BMC Biotechnology, Vol. 11, Issue 1
Tuning the expression level of recombinant proteins by modulating mRNA stability in a cell-free protein synthesis system
journal, October 2008
- Ahn, Jin-Ho; Kang, Taek-Jin; Kim, Dong-Myung
- Biotechnology and Bioengineering, Vol. 101, Issue 2
Cell-free unnatural amino acid incorporation with alternative energy systems and linear expression templates
journal, January 2014
- Shrestha, Prashanta; Smith, Mark Thomas; Bundy, Bradley Charles
- New Biotechnology, Vol. 31, Issue 1
Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization
journal, January 2003
- Griffiths, A. D.
- The EMBO Journal, Vol. 22, Issue 1
In vitro gene expression within membrane-free coacervate protocells
journal, January 2015
- Dora. Tang, T. -Y.; van Swaay, Dirk; deMello, Andrew
- Chemical Communications, Vol. 51, Issue 57
Principles of cell-free genetic circuit assembly
journal, October 2003
- Noireaux, V.; Bar-Ziv, R.; Libchaber, A.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 22
Design of a Transcriptional Biosensor for the Portable, On-Demand Detection of Cyanuric Acid
journal, December 2019
- Liu, Xiangyang; Silverman, Adam D.; Alam, Khalid K.
- ACS Synthetic Biology, Vol. 9, Issue 1
Cell-free synthesis of stable isotope-labeled internal standards for targeted quantitative proteomics
journal, June 2018
- Narumi, Ryohei; Masuda, Keiko; Tomonaga, Takeshi
- Synthetic and Systems Biotechnology, Vol. 3, Issue 2
Fabrication optimization of a miniaturized array device for cell-free protein synthesis
journal, October 2011
- Khnouf, Ruba; Chapman, Benjamin D.; Hugh Fan, Z.
- ELECTROPHORESIS, Vol. 32, Issue 22
Cell-Free Protein Synthesis From Fast-Growing Vibrio natriegens
journal, June 2018
- Failmezger, Jurek; Scholz, Steffen; Blombach, Bastian
- Frontiers in Microbiology, Vol. 9
An Integrated Device for Monitoring Time-Dependent in vitro Expression From Single Genes in Picolitre Droplets
journal, February 2008
- Courtois, Fabienne; Olguin, Luis F.; Whyte, Graeme
- ChemBioChem, Vol. 9, Issue 3
Multi-dimensional studies of synthetic genetic promoters enabled by microfluidic impact printing
journal, January 2017
- Fan, Jinzhen; Villarreal, Fernando; Weyers, Brent
- Lab on a Chip, Vol. 17, Issue 13
Expanding the palette of Streptomyces-based cell-free protein synthesis systems with enhanced yields
journal, February 2018
- Li, Jian; Wang, He; Jewett, Michael C.
- Biochemical Engineering Journal, Vol. 130
N-Linked Glycosylation in Campylobacter jejuni and Its Functional Transfer into E. coli
journal, November 2002
- Wacker, M.
- Science, Vol. 298, Issue 5599
Construction of Incoherent Feedforward Loop Circuits in a Cell-Free System and in Cells
journal, January 2019
- Guo, Shaobin; Murray, Richard M.
- ACS Synthetic Biology, Vol. 8, Issue 3
Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens
journal, August 2018
- Des Soye, Benjamin J.; Davidson, Samuel R.; Weinstock, Matthew T.
- ACS Synthetic Biology, Vol. 7, Issue 9
BioBits™ Explorer: A modular synthetic biology education kit
journal, August 2018
- Huang, Ally; Nguyen, Peter Q.; Stark, Jessica C.
- Science Advances, Vol. 4, Issue 8
Dual function of the messenger RNA cap structure in poly(A)-tail-promoted translation in yeast
journal, April 1998
- Preiss, Thomas; Hentze, Matthias W.
- Nature, Vol. 392, Issue 6675
BioBits™ Bright: A fluorescent synthetic biology education kit
journal, August 2018
- Stark, Jessica C.; Huang, Ally; Nguyen, Peter Q.
- Science Advances, Vol. 4, Issue 8
Programmable on-chip DNA compartments as artificial cells
journal, August 2014
- Karzbrun, E.; Tayar, A. M.; Noireaux, V.
- Science, Vol. 345, Issue 6198
Massively parallel measurements of molecular interaction kinetics on a microfluidic platform
journal, September 2012
- Geertz, M.; Shore, D.; Maerkl, S. J.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 41
Energizing cell-free protein synthesis with glucose metabolism
journal, January 2005
- Calhoun, Kara A.; Swartz, James R.
- Biotechnology and Bioengineering, Vol. 90, Issue 5
Preservation of protein expression systems at elevated temperatures for portable therapeutic production
journal, April 2017
- Karig, David K.; Bessling, Seneca; Thielen, Peter
- Journal of The Royal Society Interface, Vol. 14, Issue 129
Cell-free production of scFv fusion proteins: an efficient approach for personalized lymphoma vaccines
journal, December 2006
- Kanter, Gregory; Yang, Junhao; Voloshin, Alexei
- Blood, Vol. 109, Issue 8
Cell-free protein expression in a microchannel array with passive pumping
journal, January 2009
- Khnouf, Ruba; Beebe, David J.; Fan, Z. Hugh
- Lab Chip, Vol. 9, Issue 1
Toehold Switches: De-Novo-Designed Regulators of Gene Expression
journal, November 2014
- Green, Alexander A.; Silver, Pamela A.; Collins, James J.
- Cell, Vol. 159, Issue 4
Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components
journal, May 2016
- Pardee, Keith; Green, Alexander A.; Takahashi, Melissa K.
- Cell, Vol. 165, Issue 5
The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides
journal, October 1961
- Nirenberg, M. W.; Matthaei, J. H.
- Proceedings of the National Academy of Sciences, Vol. 47, Issue 10
Stochasticity in Gene Expression in a Cell-Sized Compartment
journal, September 2014
- Nishimura, Kazuya; Tsuru, Saburo; Suzuki, Hiroaki
- ACS Synthetic Biology, Vol. 4, Issue 5
Gene Circuit Performance Characterization and Resource Usage in a Cell-Free “Breadboard”
journal, April 2014
- Siegal-Gaskins, Dan; Tuza, Zoltan A.; Kim, Jongmin
- ACS Synthetic Biology, Vol. 3, Issue 6
Paper-Based Synthetic Gene Networks
journal, November 2014
- Pardee, Keith; Green, Alexander A.; Ferrante, Tom
- Cell, Vol. 159, Issue 4
Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin
journal, June 2018
- Zemella, Anne; Thoring, Lena; Hoffmeister, Christian
- Scientific Reports, Vol. 8, Issue 1
Cell-free extract based optimization of biomolecular circuits with droplet microfluidics
journal, January 2017
- Hori, Yutaka; Kantak, Chaitanya; Murray, Richard M.
- Lab on a Chip, Vol. 17, Issue 18
The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology
journal, February 2016
- Garamella, Jonathan; Marshall, Ryan; Rustad, Mark
- ACS Synthetic Biology, Vol. 5, Issue 4
Green fluorescent protein as a marker for gene expression
journal, February 1994
- Chalfie, M.; Tu, Y.; Euskirchen, G.
- Science, Vol. 263, Issue 5148
Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology
journal, January 2013
- Chappell, James; Jensen, Kirsten; Freemont, Paul S.
- Nucleic Acids Research, Vol. 41, Issue 5
A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
journal, November 2019
- Kightlinger, Weston; Duncker, Katherine E.; Ramesh, Ashvita
- Nature Communications, Vol. 10, Issue 1
Rapid Screening of Quorum-Sensing Signal N-Acyl Homoserine Lactones by an In Vitro Cell-Free Assay
journal, April 2008
- Kawaguchi, Tomohiro; Chen, Yung Pin; Norman, R. Sean
- Applied and Environmental Microbiology, Vol. 74, Issue 12
Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
journal, July 2018
- Jaroentomeechai, Thapakorn; Stark, Jessica C.; Natarajan, Aravind
- Nature Communications, Vol. 9, Issue 1
Cell-free supplement mixtures: Elucidating the history and biochemical utility of additives used to support in vitro protein synthesis in E. coli extract
journal, January 2019
- Dopp, By Jared L.; Tamiev, Denis D.; Reuel, Nigel F.
- Biotechnology Advances, Vol. 37, Issue 1
Metabolic Profiling of Escherichia coli -Based Cell-Free Expression Systems for Process Optimization
journal, September 2019
- Miguez, April M.; McNerney, Monica P.; Styczynski, Mark P.
- Industrial & Engineering Chemistry Research, Vol. 58, Issue 50
Cell-free prediction of protein expression costs for growing cells
journal, April 2018
- Borkowski, Olivier; Bricio, Carlos; Murgiano, Michela
- Nature Communications, Vol. 9, Issue 1
Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis: Improved Yeast CFPS
journal, July 2013
- Hodgman, C. Eric; Jewett, Michael C.
- Biotechnology and Bioengineering, Vol. 110, Issue 10
An Economical Method for Cell-Free Protein Synthesis using Glucose and Nucleoside Monophosphates
journal, January 2005
- Calhoun, Kara A.; Swartz, James R.
- Biotechnology Progress, Vol. 21, Issue 4
Regeneration of adenosine triphosphate from glycolytic intermediates for cell-free protein synthesis
journal, January 2001
- Kim, Dong-Myung; Swartz, James R.
- Biotechnology and Bioengineering, Vol. 74, Issue 4
Expanding biological applications using cell-free metabolic engineering: An overview
journal, November 2018
- Swartz, James R.
- Metabolic Engineering, Vol. 50
Endotoxin-Free E. coli- Based Cell-Free Protein Synthesis: Pre-Expression Endotoxin Removal Approaches for on-Demand Cancer Therapeutic Production
journal, September 2018
- Wilding, Kristen M.; Hunt, John P.; Wilkerson, Joshua W.
- Biotechnology Journal, Vol. 14, Issue 3
Cell-Free Protein Synthesis Enhancement from Real-Time NMR Metabolite Kinetics: Redirecting Energy Fluxes in Hybrid RRL Systems
journal, September 2017
- Panthu, Baptiste; Ohlmann, Théophile; Perrier, Johan
- ACS Synthetic Biology, Vol. 7, Issue 1
DNA Detection Using Recombination Proteins
journal, June 2006
- Piepenburg, Olaf; Williams, Colin H.; Stemple, Derek L.
- PLoS Biology, Vol. 4, Issue 7
Streamlining Escherichia Coli S30 Extract Preparation for Economical Cell-Free Protein Synthesis
journal, January 2005
- Liu, David V.; Zawada, James F.; Swartz, James R.
- Biotechnology Progress, Vol. 21, Issue 2
Secretion in yeast: Reconstitution of the translocation and glycosylation of α-factor and invertase in a homologous cell-free system
journal, February 1986
- Rothblatt, Jonathan A.; Meyer, David I.
- Cell, Vol. 44, Issue 4
Translation inhibition and resource balance in the TX-TL cell-free gene expression system
journal, January 2017
- Nagaraj, Vijayalakshmi H.; Greene, James M.; Sengupta, Anirvan M.
- Synthetic Biology, Vol. 2, Issue 1
Lyophilized Escherichia coli -based cell-free systems for robust, high-density, long-term storage
journal, April 2014
- Smith, Mark Thomas; Berkheimer, Scott D.
- BioTechniques, Vol. 56, Issue 4
Characterizing and prototyping genetic networks with cell-free transcription–translation reactions
journal, September 2015
- Takahashi, Melissa K.; Hayes, Clarmyra A.; Chappell, James
- Methods, Vol. 86
High-throughput preparation methods of crude extract for robust cell-free protein synthesis
journal, March 2015
- Kwon, Yong-Chan; Jewett, Michael C.
- Scientific Reports, Vol. 5, Issue 1
Development of cell-free protein synthesis platforms for disulfide bonded proteins
journal, January 2007
- Goerke, Aaron R.; Swartz, James R.
- Biotechnology and Bioengineering, Vol. 99, Issue 2
Broadening Horizons and Teaching Basic Biology Through Cell-Free Synthesis of Green Fluorescent Protein in a High School Laboratory Course
journal, July 2013
- Albayrak, Cem; Jones, K. C.; Swartz, James R.
- Journal of Science Education and Technology, Vol. 22, Issue 6
A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers
journal, August 2018
- Takahashi, Melissa K.; Tan, Xiao; Dy, Aaron J.
- Nature Communications, Vol. 9, Issue 1
Spatiotemporal control of coacervate formation within liposomes
journal, April 2019
- Deshpande, Siddharth; Brandenburg, Frank; Lau, Anson
- Nature Communications, Vol. 10, Issue 1
Short DNA containing χ sites enhances DNA stability and gene expression in E. coli cell-free transcription-translation systems: Enhancing TXTL-Based Expression With χ-Site DNA
journal, May 2017
- Marshall, Ryan; Maxwell, Colin S.; Collins, Scott P.
- Biotechnology and Bioengineering, Vol. 114, Issue 9
Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis
journal, September 1995
- Kigawa, Takanori; Muto, Yutaka; Yokoyama, Shigeyuki
- Journal of Biomolecular NMR, Vol. 6, Issue 2
Multiplex transcriptional characterizations across diverse bacterial species using cell‐free systems
journal, August 2019
- Yim, Sung Sun; Johns, Nathan I.; Park, Jimin
- Molecular Systems Biology, Vol. 15, Issue 8
Modelling cell-free RNA and protein synthesis with minimal systems
journal, January 2019
- Doerr, Anne; de Reus, Elise; van Nies, Pauline
- Physical Biology, Vol. 16, Issue 2
RNA Codewords and Protein Synthesis: The Effect of Trinucleotides upon the Binding of sRNA to Ribosomes
journal, September 1964
- Nirenberg, M.; Leder, P.
- Science, Vol. 145, Issue 3639
Simple procedures for the construction of a robust and cost-effective cell-free protein synthesis system
journal, December 2006
- Kim, Tae-Wan; Keum, Jung-Won; Oh, In-Seok
- Journal of Biotechnology, Vol. 126, Issue 4
Improving Cell-Free Protein Synthesis through Genome Engineering of Escherichia coli Lacking Release Factor 1
journal, March 2015
- Hong, Seok Hoon; Kwon, Yong-Chan; Martin, Rey W.
- ChemBioChem, Vol. 16, Issue 5
High yield cell-free production of integral membrane proteins without refolding or detergents
journal, May 2008
- Wuu, Jessica J.; Swartz, James R.
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1778, Issue 5, p. 1237-1250
Optimizing Cell-Free Biosensors to Monitor Enzymatic Production
journal, July 2019
- Pandi, Amir; Grigoras, Ioana; Borkowski, Olivier
- ACS Synthetic Biology, Vol. 8, Issue 8
Cell-free microcompartmentalised transcription–translation for the prototyping of synthetic communication networks
journal, August 2019
- Dubuc, Emilien; Pieters, Pascal A.; van der Linden, Ardjan J.
- Current Opinion in Biotechnology, Vol. 58
Cell-Free Optogenetic Gene Expression System
journal, March 2018
- Jayaraman, Premkumar; Yeoh, Jing Wui; Jayaraman, Sudhaghar
- ACS Synthetic Biology, Vol. 7, Issue 4
Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2,3-butanediol
journal, November 2015
- Kay, Jennifer E.; Jewett, Michael C.
- Metabolic Engineering, Vol. 32
Real-Time mRNA Measurement during an in Vitro Transcription and Translation Reaction Using Binary Probes
journal, December 2012
- Niederholtmeyer, Henrike; Xu, Ling; Maerkl, Sebastian J.
- ACS Synthetic Biology, Vol. 2, Issue 8
Point-of-care biomarker quantification enabled by sample-specific calibration
journal, September 2019
- McNerney, Monica P.; Zhang, Yan; Steppe, Paige
- Science Advances, Vol. 5, Issue 9
High-yield production of “difficult-to-express” proteins in a continuous exchange cell-free system based on CHO cell lysates
journal, September 2017
- Thoring, Lena; Dondapati, Srujan K.; Stech, Marlitt
- Scientific Reports, Vol. 7, Issue 1
Engineering and characterization of a superfolder green fluorescent protein
journal, December 2005
- Pédelacq, Jean-Denis; Cabantous, Stéphanie; Tran, Timothy
- Nature Biotechnology, Vol. 24, Issue 1, p. 79-88
Robust production of recombinant phosphoproteins using cell-free protein synthesis
journal, September 2015
- Oza, Javin P.; Aerni, Hans R.; Pirman, Natasha L.
- Nature Communications, Vol. 6, Issue 1
Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system
journal, November 2015
- Salehi, Amin S. M.; Smith, Mark Thomas; Bennett, Anthony M.
- Biotechnology Journal, Vol. 11, Issue 2
Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin
text, January 2020
- Zemella, Anne; Thoring, Lena; Hoffmeister, Christian
- Universität Potsdam
Quantification of Interlaboratory Cell-Free Protein Synthesis Variability
journal, August 2019
- Cole, Stephanie D.; Beabout, Kathryn; Turner, Kendrick B.
- ACS Synthetic Biology, Vol. 8, Issue 9
Increasing PCR Fragment Stability and Protein Yields in a Cell-Free System with Genetically Modified Escherichia coli Extracts
journal, January 2005
- Michel-Reydellet, Nathalie; Woodrow, Kim; Swartz, James
- Journal of Molecular Microbiology and Biotechnology, Vol. 9, Issue 1
Artificial Gel-Based Organelles for Spatial Organization of Cell-Free Gene Expression Reactions
journal, November 2018
- Aufinger, Lukas; Simmel, Friedrich C.
- Angewandte Chemie International Edition, Vol. 57, Issue 52
A Pressure Test to Make 10 Molecules in 90 Days: External Evaluation of Methods to Engineer Biology
journal, February 2018
- Casini, Arturo; Chang, Fang-Yuan; Eluere, Raissa
- Journal of the American Chemical Society, Vol. 140, Issue 12
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs
journal, November 1986
- Blow, J. Julian; Laskey, Ronald A.
- Cell, Vol. 47, Issue 4
Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool
journal, February 2017
- Moore, Simon J.; Lai, Hung-En; Needham, Hannah
- Biotechnology Journal, Vol. 12, Issue 4
Distinct timescales of RNA regulators enable the construction of a genetic pulse generator
journal, February 2019
- Westbrook, Alexandra; Tang, Xun; Marshall, Ryan
- Biotechnology and Bioengineering, Vol. 116, Issue 5
Engineering toward a bacterial “endoplasmic reticulum” for the rapid expression of immunoglobulin proteins
journal, February 2014
- Groff, Dan; Armstrong, Stephanie; Rivers, Patrick J.
- mAbs, Vol. 6, Issue 3
Substrate replenishment extends protein synthesis with an in vitro translation system designed to mimic the cytoplasm
journal, January 2004
- Jewett, Michael C.; Swartz, James R.
- Biotechnology and Bioengineering, Vol. 87, Issue 4
Quantitative modeling of transcription and translation of an all-E. coli cell-free system
journal, August 2019
- Marshall, Ryan; Noireaux, Vincent
- Scientific Reports, Vol. 9, Issue 1
A New Embedded Process for Compartmentalized Cell-Free Protein Expression and On-line Detection in Microfluidic Devices
journal, April 2005
- Dittrich, Petra S.; Jahnz, Michael; Schwille, Petra
- ChemBioChem, Vol. 6, Issue 5
Self-assembling functional programmable protein array for studying protein–protein interactions in malaria parasites
journal, July 2018
- Arévalo-Pinzón, Gabriela; González-González, María; Suárez, Carlos Fernando
- Malaria Journal, Vol. 17, Issue 1
DiscernArray™ technology: a cell-free method for the generation of protein arrays from PCR DNA
journal, March 2003
- He, Mingyue; Taussig, Michael J.
- Journal of Immunological Methods, Vol. 274, Issue 1-2
Establishing a high yielding streptomyces -based cell-free protein synthesis system: Establishing a Streptomyces -Based CFPS System
journal, February 2017
- Li, Jian; Wang, He; Kwon, Yong-Chan
- Biotechnology and Bioengineering, Vol. 114, Issue 6
Cell-free synthetic biology: Thinking outside the cell
journal, May 2012
- Hodgman, C. Eric; Jewett, Michael C.
- Metabolic Engineering, Vol. 14, Issue 3
Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors
journal, April 2019
- Voyvodic, Peter L.; Pandi, Amir; Koch, Mathilde
- Nature Communications, Vol. 10, Issue 1
Characterization of the cytoplasm of Escherichia coli K-12 as a function of external osmolarity
journal, November 1991
- Cayley, Scott; Lewis, Barbara A.; Guttman, Harry J.
- Journal of Molecular Biology, Vol. 222, Issue 2
Printing protein arrays from DNA arrays
journal, January 2008
- He, Mingyue; Stoevesandt, Oda; Palmer, Elizabeth A.
- Nature Methods, Vol. 5, Issue 2
Mimicking theEscherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis
journal, January 2004
- Jewett, Michael C.; Swartz, James R.
- Biotechnology and Bioengineering, Vol. 86, Issue 1
A sequential expression system for high-throughput functional genomic analysis
journal, November 2007
- Woodrow, Kim A.; Swartz, James R.
- PROTEOMICS, Vol. 7, Issue 21
Portable, On-Demand Biomolecular Manufacturing
journal, September 2016
- Pardee, Keith; Slomovic, Shimyn; Nguyen, Peter Q.
- Cell, Vol. 167, Issue 1
Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70
journal, January 2010
- Shin, Jonghyeon; Noireaux, Vincent
- Journal of Biological Engineering, Vol. 4, Issue 1
Strand Displacement Probes Combined with Isothermal Nucleic Acid Amplification for Instrument-Free Detection from Complex Samples
journal, April 2018
- Phillips, Elizabeth A.; Moehling, Taylor J.; Bhadra, Sanchita
- Analytical Chemistry, Vol. 90, Issue 11
Toward Microfluidic Reactors for Cell-Free Protein Synthesis at the Point-of-Care
journal, December 2015
- Timm, Andrea C.; Shankles, Peter G.; Foster, Carmen M.
- Small, Vol. 12, Issue 6
Rapid cell-free forward engineering of novel genetic ring oscillators
journal, October 2015
- Niederholtmeyer, Henrike; Sun, Zachary Z.; Hori, Yutaka
- eLife, Vol. 4
Characterization of the all‐ E. coli transcription‐translation system myTXTL by mass spectrometry
journal, May 2019
- Garenne, David; Beisel, Chase L.; Noireaux, Vincent
- Rapid Communications in Mass Spectrometry, Vol. 33, Issue 11
Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system
journal, April 2014
- Caschera, Filippo; Noireaux, Vincent
- Biochimie, Vol. 99
Automated physics-based design of synthetic riboswitches from diverse RNA aptamers
journal, November 2015
- Espah Borujeni, Amin; Mishler, Dennis M.; Wang, Jingzhi
- Nucleic Acids Research, Vol. 44, Issue 1
A novel cell-free protein synthesis system
journal, June 2004
- Sitaraman, Kalavathy; Esposito, Dominic; Klarmann, George
- Journal of Biotechnology, Vol. 110, Issue 3
Cell-free prototyping strategies for enhancing the sustainable production of polyhydroxyalkanoates bioplastics
journal, January 2018
- Kelwick, Richard; Ricci, Luca; Chee, Soo Mei
- Synthetic Biology, Vol. 3, Issue 1
Low-cost detection of norovirus using paper-based cell-free systems and synbody-based viral enrichment
journal, January 2018
- Ma, Duo; Shen, Luhui; Wu, Kaiyue
- Synthetic Biology, Vol. 3, Issue 1
Cell-free protein synthesis and assembly on a biochip
journal, May 2012
- Heyman, Yael; Buxboim, Amnon; Wolf, Sharon G.
- Nature Nanotechnology, Vol. 7, Issue 6
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System
journal, January 2018
- Marshall, Ryan; Maxwell, Colin S.; Collins, Scott P.
- Molecular Cell, Vol. 69, Issue 1
A continuous cell-free translation system capable of producing polypeptides in high yield
journal, November 1988
- Spirin, A.; Baranov, V.; Ryabova, L.
- Science, Vol. 242, Issue 4882
Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates
journal, September 2017
- Stech, M.; Nikolaeva, O.; Thoring, L.
- Scientific Reports, Vol. 7, Issue 1
Enzymatic replication of the origin of the Escherichia coli chromosome.
journal, December 1981
- Fuller, R. S.; Kaguni, J. M.; Kornberg, A.
- Proceedings of the National Academy of Sciences, Vol. 78, Issue 12
In Vitro Synthesis of Protein in Microbial Systems
journal, December 1973
- Zubay, G.
- Annual Review of Genetics, Vol. 7, Issue 1
Synthetic translational regulation by an L7Ae–kink-turn RNP switch
journal, December 2009
- Saito, Hirohide; Kobayashi, Tetsuhiro; Hara, Tomoaki
- Nature Chemical Biology, Vol. 6, Issue 1
Mass spectrometry-based absolute quantification reveals rhythmic variation of mouse circadian clock proteins
journal, May 2016
- Narumi, Ryohei; Shimizu, Yoshihiro; Ukai-Tadenuma, Maki
- Proceedings of the National Academy of Sciences, Vol. 113, Issue 24
The E. coli S30 lysate proteome: A prototype for cell-free protein production
journal, January 2018
- Foshag, Daniel; Henrich, Erik; Hiller, Ekkehard
- New Biotechnology, Vol. 40
A vesicle bioreactor as a step toward an artificial cell assembly
journal, December 2004
- Noireaux, V.; Libchaber, A.
- Proceedings of the National Academy of Sciences, Vol. 101, Issue 51
A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa -Infected Respiratory Samples
journal, October 2017
- Wen, Ke Yan; Cameron, Loren; Chappell, James
- ACS Synthetic Biology, Vol. 6, Issue 12
Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System
journal, December 2013
- Sun, Zachary Z.; Yeung, Enoch; Hayes, Clarmyra A.
- ACS Synthetic Biology, Vol. 3, Issue 6
Sequence Specific Modeling of E. coli Cell-Free Protein Synthesis
journal, June 2018
- Vilkhovoy, Michael; Horvath, Nicholas; Shih, Che-Hsiao
- ACS Synthetic Biology, Vol. 7, Issue 8
Robust Strand Exchange Reactions for the Sequence-Specific, Real-Time Detection of Nucleic Acid Amplicons
journal, March 2015
- Jiang, Yu Sherry; Bhadra, Sanchita; Li, Bingling
- Analytical Chemistry, Vol. 87, Issue 6
Development of a Pseudomonas putida cell-free protein synthesis platform for rapid screening of gene regulatory elements
journal, January 2018
- Wang, He; Li, Jian; Jewett, Michael C.
- Synthetic Biology, Vol. 3, Issue 1
Cell-free TXTL synthesis of infectious bacteriophage T4 in a single test tube reaction
journal, January 2018
- Rustad, Mark; Eastlund, Allen; Jardine, Paul
- Synthetic Biology, Vol. 3, Issue 1
Cell-Free Protein Synthesis Approach to Biosensing hTRβ-Specific Endocrine Disruptors
journal, March 2017
- Salehi, Amin S. M.; Shakalli Tang, Miriam J.; Smith, Mark T.
- Analytical Chemistry, Vol. 89, Issue 6
PLANT-Dx: A Molecular Diagnostic for Point-of-Use Detection of Plant Pathogens
journal, February 2019
- Verosloff, M.; Chappell, J.; Perry, K. L.
- ACS Synthetic Biology, Vol. 8, Issue 4
Adapting Enzyme-Free DNA Circuits to the Detection of Loop-Mediated Isothermal Amplification Reactions
journal, September 2012
- Li, Bingling; Chen, Xi; Ellington, Andrew D.
- Analytical Chemistry, Vol. 84, Issue 19
Digital-to-biological converter for on-demand production of biologics
journal, May 2017
- Boles, Kent S.; Kannan, Krishna; Gill, John
- Nature Biotechnology, Vol. 35, Issue 7
Rapid expression of vaccine proteins for B-cell lymphoma in a cell-free system
journal, January 2005
- Yang, Junhao; Kanter, Gregory; Voloshin, Alexei
- Biotechnology and Bioengineering, Vol. 89, Issue 5
A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution
journal, January 2012
- Fallah-Araghi, Ali; Baret, Jean-Christophe; Ryckelynck, Michael
- Lab on a Chip, Vol. 12, Issue 5
An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells
journal, January 2012
- Shin, Jonghyeon; Noireaux, Vincent
- ACS Synthetic Biology, Vol. 1, Issue 1
DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation
journal, January 2014
- Thiele, J.; Ma, Y.; Foschepoth, D.
- Lab on a Chip, Vol. 14, Issue 15
Man-made cell-like compartments for molecular evolution
journal, July 1998
- Tawfik, Dan S.; Griffiths, Andrew D.
- Nature Biotechnology, Vol. 16, Issue 7
Amino acid stabilization for cell-free protein synthesis by modification of the Escherichia coli genome
journal, July 2004
- Michel-Reydellet, Nathalie; Calhoun, Kara; Swartz, James
- Metabolic Engineering, Vol. 6, Issue 3
An engineered eukaryotic protein glycosylation pathway in Escherichia coli
journal, March 2012
- Valderrama-Rincon, Juan D.; Fisher, Adam C.; Merritt, Judith H.
- Nature Chemical Biology, Vol. 8, Issue 5
Towards synthetic cells using peptide-based reaction compartments
journal, September 2018
- Vogele, Kilian; Frank, Thomas; Gasser, Lukas
- Nature Communications, Vol. 9, Issue 1
An integrated cell-free metabolic platform for protein production and synthetic biology
journal, October 2008
- Jewett, Michael C.; Calhoun, Kara A.; Voloshin, Alexei
- Molecular Systems Biology, Vol. 4, Issue 1, Article No. 220
An educational module to explore CRISPR technologies with a cell-free transcription-translation system
journal, January 2019
- Collias, Daphne; Marshall, Ryan; Collins, Scott P.
- Synthetic Biology, Vol. 4, Issue 1
Cell-free biosynthesis of limonene using enzyme-enriched Escherichia coli lysates
journal, January 2019
- Dudley, Quentin M.; Nash, Connor J.; Jewett, Michael C.
- Synthetic Biology, Vol. 4, Issue 1
Systematic discovery of natural CRISPR-Cas12a inhibitors
journal, September 2018
- Watters, Kyle E.; Fellmann, Christof; Bai, Hua B.
- Science, Vol. 362, Issue 6411
Miniaturized fluid array for high-throughput protein expression
journal, November 2010
- Khnouf, Ruba; Olivero, Daniel; Jin, Shouguang
- Biotechnology Progress, Vol. 26, Issue 6
In silico assessment of cell-free systems
journal, May 2012
- Bujara, Matthias; Panke, Sven
- Biotechnology and Bioengineering, Vol. 109, Issue 10
Cell-free gene-regulatory network engineering with synthetic transcription factors
journal, March 2019
- Swank, Zoe; Laohakunakorn, Nadanai; Maerkl, Sebastian J.
- Proceedings of the National Academy of Sciences, Vol. 116, Issue 13
A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery
journal, July 2016
- Karim, Ashty S.; Jewett, Michael C.
- Metabolic Engineering, Vol. 36
Genome Replication, Synthesis, and Assembly of the Bacteriophage T7 in a Single Cell-Free Reaction
journal, July 2012
- Shin, Jonghyeon; Jardine, Paul; Noireaux, Vincent
- ACS Synthetic Biology, Vol. 1, Issue 9
Controlling cell-free metabolism through physiochemical perturbations
journal, January 2018
- Karim, Ashty S.; Heggestad, Jacob T.; Crowe, Samantha A.
- Metabolic Engineering, Vol. 45
Escherichia coli-based cell-free synthesis of virus-like particles
journal, January 2008
- Bundy, Bradley C.; Franciszkowicz, Marc J.; Swartz, James R.
- Biotechnology and Bioengineering, Vol. 100, Issue 1
Long-lived protein expression in hydrogel particles: towards artificial cells
journal, January 2018
- Zhou, Xiaoyu; Wu, Han; Cui, Miao
- Chemical Science, Vol. 9, Issue 18
Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation
journal, April 2013
- Albayrak, Cem; Swartz, James R.
- Nucleic Acids Research, Vol. 41, Issue 11
A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins
journal, November 2011
- Guarino, Cassandra; DeLisa, Matthew P.
- Glycobiology, Vol. 22, Issue 5
Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry
journal, December 2018
- Silverman, Adam D.; Kelley-Loughnane, Nancy; Lucks, Julius B.
- ACS Synthetic Biology, Vol. 8, Issue 2
In vitro gene expression within membrane-free coacervate protocells
text, January 2015
- Tang, T-Y Dora; Swaay, Dirk Van; De Mello, Andrew
- ETH Zurich
Engineering a Functional Small RNA Negative Autoregulation Network with Model-Guided Design
journal, May 2018
- Hu, Chelsea Y.; Takahashi, Melissa K.; Zhang, Yan
- ACS Synthetic Biology, Vol. 7, Issue 6
A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System
journal, January 2019
- Lavickova, Barbora; Maerkl, Sebastian J.
- ACS Synthetic Biology, Vol. 8, Issue 2
Enhancing multiple disulfide bonded protein folding in a cell-free system
journal, January 2004
- Yin, Gang; Swartz, James R.
- Biotechnology and Bioengineering, Vol. 86, Issue 2
Works referencing / citing this record:
Mutational characterization and mapping of the 70S ribosome active site
journal, February 2020
- d’Aquino, Anne E.; Azim, Tasfia; Aleksashin, Nikolay A.
- Nucleic Acids Research, Vol. 48, Issue 5
In vitro ribosome synthesis and evolution through ribosome display
journal, February 2020
- Hammerling, Michael J.; Fritz, Brian R.; Yoesep, Danielle J.
- Nature Communications, Vol. 11, Issue 1
In vitro multi-enzymatic cascades using recombinant lysates of E. coli: an emerging biocatalysis platform
journal, January 2020
- Alissandratos, Apostolos
- Biophysical Reviews, Vol. 12, Issue 1
Strategies for in vitro engineering of the translation machinery
journal, November 2019
- Hammerling, Michael J.; Krüger, Antje; Jewett, Michael C.
- Nucleic Acids Research, Vol. 48, Issue 3
In vitro ribosome synthesis and evolution through ribosome display
journal, February 2020
- Hammerling, Michael J.; Fritz, Brian R.; Yoesep, Danielle J.
- Nature Communications, Vol. 11, Issue 1
Bottom-up construction of complex biomolecular systems with cell-free synthetic biology
text, January 2020
- Laohakunakorn, Nadanai; Grasemann, Laura; Lavickova, Barbora
- Zenodo