Never the Two Shall Mix: Robust Indel Markers to Ensure the Fidelity of Two Pivotal and Closely-Related Accessions of Brachypodium distachyon
Abstract
Brachypodium distachyon is an established model for monocotyledonous plants. Numerous markers intended for gene discovery and population genetics have been designed. However to date, very few indel markers with larger and easily scored length polymorphism differences, that distinguish between the two morphologically similar and highly utilized B. distachyon accessions, Bd21, the reference genome accession, and Bd21-3, the transformation-optimal accession, are publically available. In this study, 22 indel markers were designed and utilized to produce length polymorphism differences of 150 bp or more, for easy discrimination between Bd21 and Bd21-3. When tested on four other B. distachyon accessions, one case of multiallelism was observed. It was also shown that the markers could be used to determine homozygosity and heterozygosity at specific loci in a Bd21 x Bd3-1 F2 population. The work done in this study allows researchers to maintain the fidelity of Bd21 and Bd21-3 stocks for both transgenic and nontransgenic studies. It also provides markers that can be utilized in conjunction with others already available for further research on population genetics, gene discovery and gene characterization, all of which are necessary for the relevance of B. distachyon as a model species.
- Authors:
-
[1];
[1]
- Washington State Univ., Pullman, WA (United States). Dept. of Crop and Soil Sciences
- Publication Date:
- Research Org.:
- Washington State Univ., Pullman, WA (United States).
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1628520
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Plants
- Additional Journal Information:
- Journal Volume: 8; Journal Issue: 6; Journal ID: ISSN 2223-7747
- Publisher:
- MDPI
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Plant Sciences; Brachypodium distachyon; Bd21; Bd21-3; indels
Citation Formats
Brew-Appiah, Rhoda A. T., Peracchi, Luigi M., and Sanguinet, Karen A. Never the Two Shall Mix: Robust Indel Markers to Ensure the Fidelity of Two Pivotal and Closely-Related Accessions of Brachypodium distachyon. United States: N. p., 2019.
Web. doi:10.3390/plants8060153.
Brew-Appiah, Rhoda A. T., Peracchi, Luigi M., & Sanguinet, Karen A. Never the Two Shall Mix: Robust Indel Markers to Ensure the Fidelity of Two Pivotal and Closely-Related Accessions of Brachypodium distachyon. United States. https://doi.org/10.3390/plants8060153
Brew-Appiah, Rhoda A. T., Peracchi, Luigi M., and Sanguinet, Karen A. Sat .
"Never the Two Shall Mix: Robust Indel Markers to Ensure the Fidelity of Two Pivotal and Closely-Related Accessions of Brachypodium distachyon". United States. https://doi.org/10.3390/plants8060153. https://www.osti.gov/servlets/purl/1628520.
@article{osti_1628520,
title = {Never the Two Shall Mix: Robust Indel Markers to Ensure the Fidelity of Two Pivotal and Closely-Related Accessions of Brachypodium distachyon},
author = {Brew-Appiah, Rhoda A. T. and Peracchi, Luigi M. and Sanguinet, Karen A.},
abstractNote = {Brachypodium distachyon is an established model for monocotyledonous plants. Numerous markers intended for gene discovery and population genetics have been designed. However to date, very few indel markers with larger and easily scored length polymorphism differences, that distinguish between the two morphologically similar and highly utilized B. distachyon accessions, Bd21, the reference genome accession, and Bd21-3, the transformation-optimal accession, are publically available. In this study, 22 indel markers were designed and utilized to produce length polymorphism differences of 150 bp or more, for easy discrimination between Bd21 and Bd21-3. When tested on four other B. distachyon accessions, one case of multiallelism was observed. It was also shown that the markers could be used to determine homozygosity and heterozygosity at specific loci in a Bd21 x Bd3-1 F2 population. The work done in this study allows researchers to maintain the fidelity of Bd21 and Bd21-3 stocks for both transgenic and nontransgenic studies. It also provides markers that can be utilized in conjunction with others already available for further research on population genetics, gene discovery and gene characterization, all of which are necessary for the relevance of B. distachyon as a model species.},
doi = {10.3390/plants8060153},
journal = {Plants},
number = 6,
volume = 8,
place = {United States},
year = {Sat Jun 01 00:00:00 EDT 2019},
month = {Sat Jun 01 00:00:00 EDT 2019}
}
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Works referenced in this record:
Brachypodium distachyon as a Genetic Model System
journal, November 2015
- Kellogg, Elizabeth A.
- Annual Review of Genetics, Vol. 49, Issue 1
Genome-wide scans of selection highlight the impact of biotic and abiotic constraints in natural populations of the model grass Brachypodium distachyon
journal, September 2018
- Bourgeois, Yann; Stritt, Christoph; Walser, Jean-Claude
- The Plant Journal, Vol. 96, Issue 2
Global grass (Poaceae) success underpinned by traits facilitating colonization, persistence and habitat transformation: Grass success
journal, December 2017
- Linder, H. P.; Lehmann, Caroline E. R.; Archibald, Sally
- Biological Reviews, Vol. 93, Issue 2
Solutions for a cultivated planet
journal, October 2011
- Foley, Jonathan A.; Ramankutty, Navin; Brauman, Kate A.
- Nature, Vol. 478, Issue 7369
50 years of Arabidopsis research: highlights and future directions
journal, October 2015
- Provart, Nicholas J.; Alonso, Jose; Assmann, Sarah M.
- New Phytologist, Vol. 209, Issue 3
A Draft Sequence of the Rice Genome (Oryza sativa L. ssp. indica)
journal, April 2002
- Yu, Jun
- Science, Vol. 296, Issue 5565, p. 79-92
Development of SSR markers and analysis of diversity in Turkish populations of Brachypodium distachyon
journal, January 2009
- Vogel, John P.; Tuna, Metin; Budak, Hikmet
- BMC Plant Biology, Vol. 9, Issue 1
Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.
journal, April 2017
- Beier, Sebastian; Himmelbach, Axel; Colmsee, Christian
- Scientific Data, Vol. 4, Issue 1
Sequencing and functional validation of the JGI Brachypodium distachyon T-DNA collection
journal, June 2017
- Hsia, Mon Mandy; O'Malley, Ronan; Cartwright, Amy
- The Plant Journal, Vol. 91, Issue 3
Brachypodium distachyon . A New Model System for Functional Genomics in Grasses
journal, December 2001
- Draper, John; Mur, Luis A. J.; Jenkins, Glyn
- Plant Physiology, Vol. 127, Issue 4
Brachypodium: a promising hub between model species and cereals
journal, September 2014
- Girin, Thomas; David, Laure C.; Chardin, Camille
- Journal of Experimental Botany, Vol. 65, Issue 19
Exploring natural variation for rice sheath blight resistance in Brachypodium distachyon
journal, December 2018
- Gill, Upinder S.; Lee, Seonghee; Jia, Yulin
- Plant Signaling & Behavior, Vol. 14, Issue 1
Senescence and nitrogen use efficiency in perennial grasses for forage and biofuel production
journal, August 2017
- Yang, Jiading; Udvardi, Michael
- Journal of Experimental Botany, Vol. 69, Issue 4
Considerations of AOX Functionality Revealed by Critical Motifs and Unique Domains
journal, September 2018
- Brew-Appiah, Rhoda; Sanguinet, Karen
- International Journal of Molecular Sciences, Vol. 19, Issue 10
Development of Genetic and Genomic Research Resources for , a New Model System for Grass Crop Research
journal, January 2008
- Garvin, David F.; Gu, Yong-Qiang; Hasterok, Robert
- Crop Science, Vol. 48, Issue Supplement_1
The Molecular Basis of Diversity in the Photoperiodic Flowering Responses of Arabidopsis and Rice
journal, June 2004
- Hayama, Ryosuke; Coupland, George
- Plant Physiology, Vol. 135, Issue 2
A Draft Sequence of the Rice Genome (Oryza sativa L. ssp. japonica)
journal, April 2002
- Goff, S. A.
- Science, Vol. 296, Issue 5565
Fine Mapping of the Bsr1 Barley Stripe Mosaic Virus Resistance Gene in the Model Grass Brachypodium distachyon
journal, June 2012
- Cui, Yu; Lee, Mi Yeon; Huo, Naxin
- PLoS ONE, Vol. 7, Issue 6
A DNA Barcoding Method to Discriminate between the Model Plant Brachypodium distachyon and Its Close Relatives B. stacei and B. hybridum (Poaceae)
journal, December 2012
- López-Alvarez, Diana; López-Herranz, Maria Luisa; Betekhtin, Alexander
- PLoS ONE, Vol. 7, Issue 12
What makes a model organism?
journal, December 2013
- Leonelli, Sabina; Ankeny, Rachel A.
- Endeavour, Vol. 37, Issue 4
Triticeae Resources in Ensembl Plants
journal, November 2014
- Bolser, Dan M.; Kerhornou, Arnaud; Walts, Brandon
- Plant and Cell Physiology, Vol. 56, Issue 1
The development of Arabidopsis as a model plant
journal, March 2010
- Koornneef, Maarten; Meinke, David
- The Plant Journal, Vol. 61, Issue 6
Large Differences in Gene Expression Responses to Drought and Heat Stress between Elite Barley Cultivar Scarlett and a Spanish Landrace
journal, May 2017
- Cantalapiedra, Carlos P.; García-Pereira, María J.; Gracia, María P.
- Frontiers in Plant Science, Vol. 8
Banana21: From Gene Discovery to Deregulated Golden Bananas
journal, April 2018
- Paul, Jean-Yves; Harding, Robert; Tushemereirwe, Wilberforce
- Frontiers in Plant Science, Vol. 9
Effect of warming temperatures on US wheat yields
journal, May 2015
- Tack, Jesse; Barkley, Andrew; Nalley, Lawton Lanier
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 22
Molecular Breeding for Improved Second Generation Bioenergy Crops
journal, January 2016
- Allwright, Mike R.; Taylor, Gail
- Trends in Plant Science, Vol. 21, Issue 1
R ICE AS A M ODEL FOR C OMPARATIVE G ENOMICS OF P LANTS
journal, June 2002
- Shimamoto, Ko; Kyozuka, Junko
- Annual Review of Plant Biology, Vol. 53, Issue 1
Selection and phenotypic characterization of a core collection of Brachypodium distachyon inbred lines
journal, January 2014
- Tyler, Ludmila; Fangel, Jonatan U.; Fagerström, Alexandra
- BMC Plant Biology, Vol. 14, Issue 1
Targeting environmental adaptation in the monocot model Brachypodium distachyon: a multi-faceted approach
journal, January 2014
- Dell’Acqua, Matteo; Zuccolo, Andrea; Tuna, Metin
- BMC Genomics, Vol. 15, Issue 1
Growth and flowering physiology, and developing new technologies to increase the flower numbers in the Genus Lilium
journal, October 2013
- Suh, Jeung Keun; Wu, Xue Wei; Lee, Ae Kyung
- Horticulture, Environment, and Biotechnology, Vol. 54, Issue 5
HorTILLUS—A Rich and Renewable Source of Induced Mutations for Forward/Reverse Genetics and Pre-breeding Programs in Barley (Hordeum vulgare L.)
journal, February 2018
- Szurman-Zubrzycka, Miriam E.; Zbieszczyk, Justyna; Marzec, Marek
- Frontiers in Plant Science, Vol. 9
Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection
journal, September 2012
- Bragg, Jennifer N.; Wu, Jiajie; Gordon, Sean P.
- PLoS ONE, Vol. 7, Issue 9
Brachypodium : A Monocot Grass Model Genus for Plant Biology
journal, July 2018
- Scholthof, Karen-Beth G.; Irigoyen, Sonia; Catalan, Pilar
- The Plant Cell, Vol. 30, Issue 8
Primer3Plus, an enhanced web interface to Primer3
journal, May 2007
- Untergasser, A.; Nijveen, H.; Rao, X.
- Nucleic Acids Research, Vol. 35, Issue Web Server
Genome-wide identification and analysis of the ALTERNATIVE OXIDASE gene family in diploid and hexaploid wheat
journal, August 2018
- Brew-Appiah, Rhoda A. T.; York, Zara B.; Krishnan, Vandhana
- PLOS ONE, Vol. 13, Issue 8
Cell Wall Composition and Biomass Recalcitrance Differences Within a Genotypically Diverse Set of Brachypodium distachyon Inbred Lines
journal, May 2016
- Cass, Cynthia L.; Lavell, Anastasiya A.; Santoro, Nicholas
- Frontiers in Plant Science, Vol. 7
Comparison of a high-density genetic linkage map to genome features in the model grass Brachypodium distachyon
journal, May 2011
- Huo, Naxin; Garvin, David F.; You, Frank M.
- Theoretical and Applied Genetics, Vol. 123, Issue 3
Interaction of Photoperiod and Vernalization Determines Flowering Time of Brachypodium distachyon
journal, December 2013
- Ream, Thomas S.; Woods, Daniel P.; Schwartz, Christopher J.
- Plant Physiology, Vol. 164, Issue 2
Field Guide to Plant Model Systems
journal, October 2016
- Chang, Caren; Bowman, John L.; Meyerowitz, Elliot M.
- Cell, Vol. 167, Issue 2
Natural Variation of Flowering Time and Vernalization Responsiveness in Brachypodium distachyon
journal, February 2010
- Schwartz, Christopher J.; Doyle, Mark R.; Manzaneda, Antonio J.
- BioEnergy Research, Vol. 3, Issue 1
Validation of microsatellite markers for cytotype discrimination in the model grass Brachypodium distachyon
journal, July 2012
- Giraldo, Patricia; Rodríguez-Quijano, Marta; Vázquez, José F.
- Genome, Vol. 55, Issue 7
Neutral molecular markers support common origin of aluminium tolerance in three congeneric grass species growing in acidic soils
journal, November 2017
- Contreras, Roberto; Figueiras, Ana M.; Gallego, F. Javier
- AoB PLANTS, Vol. 9, Issue 6
Biotechnology of oil palm: Current status of oil palm genetic transformation
journal, July 2018
- Masani, Mat Yunus Abdul; Izawati, Abang Masli Dayang; Rasid, Omar Abdul
- Biocatalysis and Agricultural Biotechnology, Vol. 15
BARLEX – the Barley Draft Genome Explorer
journal, June 2015
- Colmsee, Christian; Beier, Sebastian; Himmelbach, Axel
- Molecular Plant, Vol. 8, Issue 6
Abiotic stress miRNomes in the Triticeae
journal, September 2016
- Alptekin, Burcu; Langridge, Peter; Budak, Hikmet
- Functional & Integrative Genomics, Vol. 17, Issue 2-3
Unlocking Triticeae genomics to sustainably feed the future
journal, November 2013
- Mochida, K.; Shinozaki, K.
- Plant and Cell Physiology, Vol. 54, Issue 12
Hotspots in the genomic architecture of field drought responses in wheat as breeding targets
journal, November 2018
- Gálvez, Sergio; Mérida-García, Rosa; Camino, Carlos
- Functional & Integrative Genomics, Vol. 19, Issue 2
Rice Genome Research: Current Status and Future Perspectives
journal, November 2008
- Han, Bin; Zhang, Qifa
- The Plant Genome, Vol. 1, Issue 2
Exploiting the Brachypodium Tool Box in cereal and grass research
journal, May 2011
- Mur, Luis A. J.; Allainguillaume, Joel; Catalán, Pilar
- New Phytologist, Vol. 191, Issue 2
Grasses as a Single Genetic System. Reassessment 2001
journal, March 2001
- Freeling, Michael
- Plant Physiology, Vol. 125, Issue 3
PHYTOCHROME C Is an Essential Light Receptor for Photoperiodic Flowering in the Temperate Grass, Brachypodium distachyon
journal, July 2014
- Woods, Daniel P.; Ream, Thomas S.; Minevich, Gregory
- Genetics, Vol. 198, Issue 1
Barley Genes as Tools to Confer Abiotic Stress Tolerance in Crops
journal, August 2016
- Gürel, Filiz; Öztürk, Zahide N.; Uçarlı, Cüneyt
- Frontiers in Plant Science, Vol. 7
Brachypodium distachyon and Setaria viridis : Model Genetic Systems for the Grasses
journal, April 2015
- Brutnell, Thomas P.; Bennetzen, Jeffrey L.; Vogel, John P.
- Annual Review of Plant Biology, Vol. 66, Issue 1
The B73 Maize Genome: Complexity, Diversity, and Dynamics
journal, November 2009
- Schnable, P. S.; Ware, D.; Fulton, R. S.
- Science, Vol. 326, Issue 5956, p. 1112-1115
High-efficiency Agrobacterium-mediated transformation of Brachypodium distachyon inbred line Bd21-3
journal, November 2007
- Vogel, John; Hill, Theresa
- Plant Cell Reports, Vol. 27, Issue 3
A simple and rapid method for the preparation of plant genomic DNA for PCR analysis
journal, January 1991
- Edwards, K.; Johnstone, C.; Thompson, C.
- Nucleic Acids Research, Vol. 19, Issue 6
Indel Group in Genomes (IGG) Molecular Genetic Markers
journal, July 2016
- Toal, Ted W.; Burkart-Waco, Diana; Howell, Tyson
- Plant Physiology, Vol. 172, Issue 1
A review of the trade in orchids and its implications for conservation
journal, December 2017
- Hinsley, Amy; de Boer, Hugo J.; Fay, Michael F.
- Botanical Journal of the Linnean Society, Vol. 186, Issue 4
Genome diversity in Brachypodium distachyon: deep sequencing of highly diverse inbred lines
journal, July 2014
- Gordon, Sean P.; Priest, Henry; Des Marais, David L.
- The Plant Journal, Vol. 79, Issue 3
Comparison of a high-density genetic linkage map to genome features in the model grass Brachypodium distachyon
journal, May 2011
- Huo, Naxin; Garvin, David F.; You, Frank M.
- Theoretical and Applied Genetics, Vol. 123, Issue 3
Abiotic stress miRNomes in the Triticeae
journal, September 2016
- Alptekin, Burcu; Langridge, Peter; Budak, Hikmet
- Functional & Integrative Genomics, Vol. 17, Issue 2-3
Hotspots in the genomic architecture of field drought responses in wheat as breeding targets
journal, November 2018
- Gálvez, Sergio; Mérida-García, Rosa; Camino, Carlos
- Functional & Integrative Genomics, Vol. 19, Issue 2
Natural Variation of Flowering Time and Vernalization Responsiveness in Brachypodium distachyon
journal, February 2010
- Schwartz, Christopher J.; Doyle, Mark R.; Manzaneda, Antonio J.
- BioEnergy Research, Vol. 3, Issue 1
Growth and flowering physiology, and developing new technologies to increase the flower numbers in the Genus Lilium
journal, October 2013
- Suh, Jeung Keun; Wu, Xue Wei; Lee, Ae Kyung
- Horticulture, Environment, and Biotechnology, Vol. 54, Issue 5
Grasses as a single genetic system: genome composition, collinearity and compatibility
journal, August 1993
- Bennetzin, Jeffrey L.; Freeling, Michael
- Trends in Genetics, Vol. 9, Issue 8
Biotechnology of oil palm: Current status of oil palm genetic transformation
journal, July 2018
- Masani, Mat Yunus Abdul; Izawati, Abang Masli Dayang; Rasid, Omar Abdul
- Biocatalysis and Agricultural Biotechnology, Vol. 15
What makes a model organism?
journal, December 2013
- Leonelli, Sabina; Ankeny, Rachel A.
- Endeavour, Vol. 37, Issue 4
BARLEX – the Barley Draft Genome Explorer
journal, June 2015
- Colmsee, Christian; Beier, Sebastian; Himmelbach, Axel
- Molecular Plant, Vol. 8, Issue 6
Molecular Breeding for Improved Second Generation Bioenergy Crops
journal, January 2016
- Allwright, Mike R.; Taylor, Gail
- Trends in Plant Science, Vol. 21, Issue 1
Chemistry and biological activity of steroidal glycosides from the Lilium genus
journal, January 2015
- Munafo Jr, John P.; Gianfagna, Thomas J.
- Natural Product Reports, Vol. 32, Issue 3
Effect of warming temperatures on US wheat yields
journal, May 2015
- Tack, Jesse; Barkley, Andrew; Nalley, Lawton Lanier
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 22
Neutral molecular markers support common origin of aluminium tolerance in three congeneric grass species growing in acidic soils
journal, November 2017
- Contreras, Roberto; Figueiras, Ana M.; Gallego, F. Javier
- AoB PLANTS, Vol. 9, Issue 6
A review of the trade in orchids and its implications for conservation
journal, December 2017
- Hinsley, Amy; de Boer, Hugo J.; Fay, Michael F.
- Botanical Journal of the Linnean Society, Vol. 186, Issue 4
Senescence and nitrogen use efficiency in perennial grasses for forage and biofuel production
journal, August 2017
- Yang, Jiading; Udvardi, Michael
- Journal of Experimental Botany, Vol. 69, Issue 4
A simple and rapid method for the preparation of plant genomic DNA for PCR analysis
journal, January 1991
- Edwards, K.; Johnstone, C.; Thompson, C.
- Nucleic Acids Research, Vol. 19, Issue 6
Primer3Plus, an enhanced web interface to Primer3
journal, May 2007
- Untergasser, A.; Nijveen, H.; Rao, X.
- Nucleic Acids Research, Vol. 35, Issue Web Server
Unlocking Triticeae genomics to sustainably feed the future
journal, November 2013
- Mochida, K.; Shinozaki, K.
- Plant and Cell Physiology, Vol. 54, Issue 12
Brachypodium distachyon . A New Model System for Functional Genomics in Grasses
journal, December 2001
- Draper, John; Mur, Luis A. J.; Jenkins, Glyn
- Plant Physiology, Vol. 127, Issue 4
Interaction of Photoperiod and Vernalization Determines Flowering Time of Brachypodium distachyon
journal, December 2013
- Ream, Thomas S.; Woods, Daniel P.; Schwartz, Christopher J.
- Plant Physiology, Vol. 164, Issue 2
Grasses as a Single Genetic System. Reassessment 2001
journal, March 2001
- Freeling, Michael
- Plant Physiology, Vol. 125, Issue 3
Indel Group in Genomes (IGG) Molecular Genetic Markers
journal, July 2016
- Toal, Ted W.; Burkart-Waco, Diana; Howell, Tyson
- Plant Physiology, Vol. 172, Issue 1
Brachypodium : A Monocot Grass Model Genus for Plant Biology
journal, July 2018
- Scholthof, Karen-Beth G.; Irigoyen, Sonia; Catalan, Pilar
- The Plant Cell, Vol. 30, Issue 8
Global grass (Poaceae) success underpinned by traits facilitating colonization, persistence and habitat transformation: Grass success
journal, December 2017
- Linder, H. P.; Lehmann, Caroline E. R.; Archibald, Sally
- Biological Reviews, Vol. 93, Issue 2
Exploiting the Brachypodium Tool Box in cereal and grass research
journal, May 2011
- Mur, Luis A. J.; Allainguillaume, Joel; Catalán, Pilar
- New Phytologist, Vol. 191, Issue 2
Genome diversity in Brachypodium distachyon: deep sequencing of highly diverse inbred lines
journal, July 2014
- Gordon, Sean P.; Priest, Henry; Des Marais, David L.
- The Plant Journal, Vol. 79, Issue 3
Sequencing and functional validation of the JGI Brachypodium distachyon T-DNA collection
journal, June 2017
- Hsia, Mon Mandy; O'Malley, Ronan; Cartwright, Amy
- The Plant Journal, Vol. 91, Issue 3
The B73 Maize Genome: Complexity, Diversity, and Dynamics
journal, November 2009
- Schnable, P. S.; Ware, D.; Fulton, R. S.
- Science, Vol. 326, Issue 5956, p. 1112-1115
Molecular, morphological, and cytological analysis of diverse Brachypodium distachyon inbred lines
journal, October 2009
- Filiz, E.; Ozdemir, B. S.; Budak, F.
- Genome, Vol. 52, Issue 10
Validation of microsatellite markers for cytotype discrimination in the model grass Brachypodium distachyon
journal, July 2012
- Giraldo, Patricia; Rodríguez-Quijano, Marta; Vázquez, José F.
- Genome, Vol. 55, Issue 7
Brachypodium distachyon and Setaria viridis : Model Genetic Systems for the Grasses
journal, April 2015
- Brutnell, Thomas P.; Bennetzen, Jeffrey L.; Vogel, John P.
- Annual Review of Plant Biology, Vol. 66, Issue 1
Brachypodium distachyon as a Genetic Model System
journal, November 2015
- Kellogg, Elizabeth A.
- Annual Review of Genetics, Vol. 49, Issue 1
R ICE AS A M ODEL FOR C OMPARATIVE G ENOMICS OF P LANTS
journal, June 2002
- Shimamoto, Ko; Kyozuka, Junko
- Annual Review of Plant Biology, Vol. 53, Issue 1
Targeting environmental adaptation in the monocot model Brachypodium distachyon: a multi-faceted approach
journal, January 2014
- Dell’Acqua, Matteo; Zuccolo, Andrea; Tuna, Metin
- BMC Genomics, Vol. 15, Issue 1
Selection and phenotypic characterization of a core collection of Brachypodium distachyon inbred lines
journal, January 2014
- Tyler, Ludmila; Fangel, Jonatan U.; Fagerström, Alexandra
- BMC Plant Biology, Vol. 14, Issue 1
Development of SSR markers and analysis of diversity in Turkish populations of Brachypodium distachyon
journal, January 2009
- Vogel, John P.; Tuna, Metin; Budak, Hikmet
- BMC Plant Biology, Vol. 9, Issue 1
Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection
journal, September 2012
- Bragg, Jennifer N.; Wu, Jiajie; Gordon, Sean P.
- PLoS ONE, Vol. 7, Issue 9
A DNA Barcoding Method to Discriminate between the Model Plant Brachypodium distachyon and Its Close Relatives B. stacei and B. hybridum (Poaceae)
journal, December 2012
- López-Alvarez, Diana; López-Herranz, Maria Luisa; Betekhtin, Alexander
- PLoS ONE, Vol. 7, Issue 12
PHYTOCHROME C Is an Essential Light Receptor for Photoperiodic Flowering in the Temperate Grass, Brachypodium distachyon
journal, July 2014
- Woods, Daniel P.; Ream, Thomas S.; Minevich, Gregory
- Genetics, Vol. 198, Issue 1
50 years of Arabidopsis research: Highlights and future directions
text, January 2016
- S., Cutler,; J., Waese,; S. M., Brady,
- Blackwell Publishing Ltd
Development of Genetic and Genomic Research Resources for , a New Model System for Grass Crop Research
journal, January 2008
- Garvin, David F.; Gu, Yong-Qiang; Hasterok, Robert
- Crop Science, Vol. 48, Issue Supplement_1
Cell Wall Composition and Biomass Recalcitrance Differences Within a Genotypically Diverse Set of Brachypodium distachyon Inbred Lines
journal, May 2016
- Cass, Cynthia L.; Lavell, Anastasiya A.; Santoro, Nicholas
- Frontiers in Plant Science, Vol. 7
Barley Genes as Tools to Confer Abiotic Stress Tolerance in Crops
journal, August 2016
- Gürel, Filiz; Öztürk, Zahide N.; Uçarlı, Cüneyt
- Frontiers in Plant Science, Vol. 7
Large Differences in Gene Expression Responses to Drought and Heat Stress between Elite Barley Cultivar Scarlett and a Spanish Landrace
journal, May 2017
- Cantalapiedra, Carlos P.; García-Pereira, María J.; Gracia, María P.
- Frontiers in Plant Science, Vol. 8
HorTILLUS—A Rich and Renewable Source of Induced Mutations for Forward/Reverse Genetics and Pre-breeding Programs in Barley (Hordeum vulgare L.)
journal, February 2018
- Szurman-Zubrzycka, Miriam E.; Zbieszczyk, Justyna; Marzec, Marek
- Frontiers in Plant Science, Vol. 9
Considerations of AOX Functionality Revealed by Critical Motifs and Unique Domains
journal, September 2018
- Brew-Appiah, Rhoda; Sanguinet, Karen
- International Journal of Molecular Sciences, Vol. 19, Issue 10
Rice Genome Research: Current Status and Future Perspectives
journal, November 2008
- Han, Bin; Zhang, Qifa
- The Plant Genome, Vol. 1, Issue 2