Age-related gene expression in luminal epithelial cells is driven by a microenvironment made from myoepithelial cells
Abstract
Luminal epithelial cells in the breast gradually alter gene and protein expression with age, appearing to lose lineage-specificity by acquiring myoepithelial-like characteristics. We hypothesize that the luminal lineage is particularly sensitive to microenvironment changes, and age-related microenvironment changes cause altered luminal cell phenotypes. To evaluate the effects of different microenvironments on the fidelity of epigenetically regulated luminal and myoepithelial gene expression, we generated a set of lineage-specific probes for genes that are controlled through DNA methylation. Culturing primary luminal cells under conditions that favor myoepithelial propogation led to their reprogramming at the level of gene methylation, and to a more myoepithelial-like expression profile. Primary luminal cells’ lineage-specific gene expression could be maintained when they were cultured as bilayers with primary myoepithelial cells. Isogenic stromal fibroblast co-cultures were unable to maintain the luminal phenotype. Mixed-age luminal-myoepithelial bilayers revealed that luminal cells adopt transcription and methylation patterns consistent with the chronological age of the myoepithelial cells. We provide evidence that the luminal epithelial phenotype is exquisitely sensitive to microenvironment conditions, and that states of aging are cell non-autonomously communicated through microenvironment cues over at least one cell diameter.
- Authors:
-
- City of Hope, Duarte, CA (United States). Dept. of Population Sciences
- City of Hope, Duarte, CA (United States). Dept. of Population Sciences; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Genomics and Systems Biology Division
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Genomics and Systems Biology Division; Univ. of Birmingham (United Kingdom). Dept. of Environmental Bioinformatics
- City of Hope, Duarte, CA (United States). Dept. of Population Sciences; City of Hope, Duarte, CA (United States). Center for Cancer and Aging; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division; Univ. of Bergen (Norway). Center for Cancer Biomarkers Research
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1627972
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Aging
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 10; Journal ID: ISSN 1945-4589
- Publisher:
- Impact Journals
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Cell Biology; Geriatrics & Gerontology
Citation Formats
Miyano, Masaru, Sayaman, Rosalyn W., Stoiber, Marcus H., Lin, Chun-Han, Stampfer, Martha R., Brown, James B., and LaBarge, Mark A. Age-related gene expression in luminal epithelial cells is driven by a microenvironment made from myoepithelial cells. United States: N. p., 2017.
Web. doi:10.18632/aging.101298.
Miyano, Masaru, Sayaman, Rosalyn W., Stoiber, Marcus H., Lin, Chun-Han, Stampfer, Martha R., Brown, James B., & LaBarge, Mark A. Age-related gene expression in luminal epithelial cells is driven by a microenvironment made from myoepithelial cells. United States. https://doi.org/10.18632/aging.101298
Miyano, Masaru, Sayaman, Rosalyn W., Stoiber, Marcus H., Lin, Chun-Han, Stampfer, Martha R., Brown, James B., and LaBarge, Mark A. Mon .
"Age-related gene expression in luminal epithelial cells is driven by a microenvironment made from myoepithelial cells". United States. https://doi.org/10.18632/aging.101298. https://www.osti.gov/servlets/purl/1627972.
@article{osti_1627972,
title = {Age-related gene expression in luminal epithelial cells is driven by a microenvironment made from myoepithelial cells},
author = {Miyano, Masaru and Sayaman, Rosalyn W. and Stoiber, Marcus H. and Lin, Chun-Han and Stampfer, Martha R. and Brown, James B. and LaBarge, Mark A.},
abstractNote = {Luminal epithelial cells in the breast gradually alter gene and protein expression with age, appearing to lose lineage-specificity by acquiring myoepithelial-like characteristics. We hypothesize that the luminal lineage is particularly sensitive to microenvironment changes, and age-related microenvironment changes cause altered luminal cell phenotypes. To evaluate the effects of different microenvironments on the fidelity of epigenetically regulated luminal and myoepithelial gene expression, we generated a set of lineage-specific probes for genes that are controlled through DNA methylation. Culturing primary luminal cells under conditions that favor myoepithelial propogation led to their reprogramming at the level of gene methylation, and to a more myoepithelial-like expression profile. Primary luminal cells’ lineage-specific gene expression could be maintained when they were cultured as bilayers with primary myoepithelial cells. Isogenic stromal fibroblast co-cultures were unable to maintain the luminal phenotype. Mixed-age luminal-myoepithelial bilayers revealed that luminal cells adopt transcription and methylation patterns consistent with the chronological age of the myoepithelial cells. We provide evidence that the luminal epithelial phenotype is exquisitely sensitive to microenvironment conditions, and that states of aging are cell non-autonomously communicated through microenvironment cues over at least one cell diameter.},
doi = {10.18632/aging.101298},
journal = {Aging},
number = 10,
volume = 9,
place = {United States},
year = {Mon Oct 09 00:00:00 EDT 2017},
month = {Mon Oct 09 00:00:00 EDT 2017}
}
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