Phylogenetic and Functional Diversity of Microbial Communities Associated with Subsurface Sediments of the Sonora Margin, Guaymas Basin
Abstract
Subsurface sediments of the Sonora Margin (Guaymas Basin), located in proximity of active cold seep sites were explored. The taxonomic and functional diversity of bacterial and archaeal communities were investigated from 1 to 10 meters below the seafloor. Microbial community structure and abundance and distribution of dominant populations were assessed using complementary molecular approaches (Ribosomal Intergenic Spacer Analysis, 16S rRNA libraries and quantitative PCR with an extensive primers set) and correlated to comprehensive geochemical data. Moreover the metabolic potentials and functional traits of the microbial community were also identified using the GeoChip functional gene microarray and metabolic rates. The active microbial community structure in the Sonora Margin sediments was related to deep subsurface ecosystems (Marine Benthic Groups B and D, Miscellaneous Crenarchaeotal Group, Chloroflexi and Candidate divisions) and remained relatively similar throughout the sediment section, despite defined biogeochemical gradients. However, relative abundances of bacterial and archaeal dominant lineages were significantly correlated with organic carbon quantity and origin. Consistently, metabolic pathways for the degradation and assimilation of this organic carbon as well as genetic potentials for the transformation of detrital organic matters, hydrocarbons and recalcitrant substrates were detected, suggesting that chemoorganotrophic microorganisms may dominate the microbial community of the Sonora Marginmore »
- Authors:
-
- UMR6197, ZI de la pointe du Diable, Plouzane (France). Laboratoire de Microbiologie des Environnements Extremes. Ifremer; Universite de Bretagne Occidentale, Plouzane (France). UMR6197, ZI de la pointe du Diable. Laboratoire de Microbiologie des Environnements Extreme. Centre National de la Recherche Scientifique (CNRS)
- Cardiff University (United Kingdom)
- UMR6197, ZI de la pointe du Diable, Plouzane (France). Laboratoire Etude des Environnements Profonds. Ifremer
- UMR6197, ZI de la pointe du Diable, Plouzane (France). Laboratoire de Microbiologie des Environnements Extremes. Ifremer; Universite de Bretagne Occidentale, Plouzane (France). UMR6197, ZI de la pointe du Diable. Laboratoire de Microbiologie des Environnements Extreme. Centre National de la Recherche Scientifique (CNRS); Universite de Brest, Plouzane (France). Domaines Oce´aniques IUEM. UMR6538
- ZI de la pointe du Diable, Plouzane (France). Laboratoire des Environnements Sedimentaires. Geosciences Marines. Ifremer
- University of Oklahoma, Norman, OK (United States)
- University of Oklahoma, Norman, OK (United States); Tsinghua University, Beijing (China); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); French Research Institute for Exploitation of the Sea (IFREMER)
- OSTI Identifier:
- 1627721
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 8; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; sediment; ribosomal RNA; Archaean biology; bacteria; metabolic pathways; methane; cold seeps; community structure
Citation Formats
Vigneron, Adrien, Cruaud, Perrine, Roussel, Erwan G., Pignet, Patricia, Caprais, Jean-Claude, Callac, Nolwenn, Ciobanu, Maria-Cristina, Godfroy, Anne, Cragg, Barry A., Parkes, John R., Van Nostrand, Joy D., He, Zhili, Zhou, Jizhong, and Toffin, Laurent. Phylogenetic and Functional Diversity of Microbial Communities Associated with Subsurface Sediments of the Sonora Margin, Guaymas Basin. United States: N. p., 2014.
Web. doi:10.1371/journal.pone.0104427.
Vigneron, Adrien, Cruaud, Perrine, Roussel, Erwan G., Pignet, Patricia, Caprais, Jean-Claude, Callac, Nolwenn, Ciobanu, Maria-Cristina, Godfroy, Anne, Cragg, Barry A., Parkes, John R., Van Nostrand, Joy D., He, Zhili, Zhou, Jizhong, & Toffin, Laurent. Phylogenetic and Functional Diversity of Microbial Communities Associated with Subsurface Sediments of the Sonora Margin, Guaymas Basin. United States. https://doi.org/10.1371/journal.pone.0104427
Vigneron, Adrien, Cruaud, Perrine, Roussel, Erwan G., Pignet, Patricia, Caprais, Jean-Claude, Callac, Nolwenn, Ciobanu, Maria-Cristina, Godfroy, Anne, Cragg, Barry A., Parkes, John R., Van Nostrand, Joy D., He, Zhili, Zhou, Jizhong, and Toffin, Laurent. Wed .
"Phylogenetic and Functional Diversity of Microbial Communities Associated with Subsurface Sediments of the Sonora Margin, Guaymas Basin". United States. https://doi.org/10.1371/journal.pone.0104427. https://www.osti.gov/servlets/purl/1627721.
@article{osti_1627721,
title = {Phylogenetic and Functional Diversity of Microbial Communities Associated with Subsurface Sediments of the Sonora Margin, Guaymas Basin},
author = {Vigneron, Adrien and Cruaud, Perrine and Roussel, Erwan G. and Pignet, Patricia and Caprais, Jean-Claude and Callac, Nolwenn and Ciobanu, Maria-Cristina and Godfroy, Anne and Cragg, Barry A. and Parkes, John R. and Van Nostrand, Joy D. and He, Zhili and Zhou, Jizhong and Toffin, Laurent},
abstractNote = {Subsurface sediments of the Sonora Margin (Guaymas Basin), located in proximity of active cold seep sites were explored. The taxonomic and functional diversity of bacterial and archaeal communities were investigated from 1 to 10 meters below the seafloor. Microbial community structure and abundance and distribution of dominant populations were assessed using complementary molecular approaches (Ribosomal Intergenic Spacer Analysis, 16S rRNA libraries and quantitative PCR with an extensive primers set) and correlated to comprehensive geochemical data. Moreover the metabolic potentials and functional traits of the microbial community were also identified using the GeoChip functional gene microarray and metabolic rates. The active microbial community structure in the Sonora Margin sediments was related to deep subsurface ecosystems (Marine Benthic Groups B and D, Miscellaneous Crenarchaeotal Group, Chloroflexi and Candidate divisions) and remained relatively similar throughout the sediment section, despite defined biogeochemical gradients. However, relative abundances of bacterial and archaeal dominant lineages were significantly correlated with organic carbon quantity and origin. Consistently, metabolic pathways for the degradation and assimilation of this organic carbon as well as genetic potentials for the transformation of detrital organic matters, hydrocarbons and recalcitrant substrates were detected, suggesting that chemoorganotrophic microorganisms may dominate the microbial community of the Sonora Margin subsurface sediments.},
doi = {10.1371/journal.pone.0104427},
journal = {PLoS ONE},
number = 8,
volume = 9,
place = {United States},
year = {Wed Aug 06 00:00:00 EDT 2014},
month = {Wed Aug 06 00:00:00 EDT 2014}
}
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