Crystal structure of a DNA polymerase sliding clamp from a Gram-positive bacterium
Abstract
Background: Sliding DNA clamps are processivity factors that are required for efficient DNA replication. DNA polymerases maintain proximity to nucleic acid templates by interacting with sliding clamps that encircle DNA and thereby link the polymerase enzyme to the DNA substrate. Although the structures of sliding clamps from Gram-negative bacteria (E. coli), eukaryotes, archaea, and T4-like bacteriophages are well-known, the structure of a sliding clamp from Grampositive bacteria has not been reported previously. Results: We have determined the crystal structure of the dimeric β subunit of the DNA polymerase III holoenzyme of Streptococcus pyogenes. The sliding clamp from this Gram-positive organism forms a ring-shaped dimeric assembly that is similar in overall structure to that of the sliding clamps from Gram-negative bacteria, bacteriophage T4, eukaryotes and archaea. The dimer has overall dimensions of ~90 Å × ~70 Å × ~25 Å with a central chamber that is large enough to accommodate duplex DNA. In comparison to the circular shape of other assemblies, the S. pyogenes clamp adopts a more elliptical structure. Conclusion: The sequences of sliding clamps from S. pyogenes and E. coli are only 23% identical, making the generation of structural models for the S. pyogenes clamp difficult in the absencemore »
- Authors:
-
- Rockefeller Univ., New York, NY (United States). Howard Hughes Medical Inst.
- Univ. of California, Berkeley, CA (United States). Howard Hughes Medical Inst. Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; National Institutes of Health (NIH)
- OSTI Identifier:
- 1626550
- Grant/Contract Number:
- AC02-05CH11231; GM45547; (GM38839
- Resource Type:
- Accepted Manuscript
- Journal Name:
- BMC Structural Biology (Online)
- Additional Journal Information:
- Journal Name: BMC Structural Biology (Online); Journal Volume: 6; Journal Issue: 1; Journal ID: ISSN 1472-6807
- Publisher:
- BioMed Central
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Biophysics
Citation Formats
Argiriadi, Maria A., Goedken, Eric R., Bruck, Irina, O'Donnell, Mike, and Kuriyan, John. Crystal structure of a DNA polymerase sliding clamp from a Gram-positive bacterium. United States: N. p., 2006.
Web. doi:10.1186/1472-6807-6-2.
Argiriadi, Maria A., Goedken, Eric R., Bruck, Irina, O'Donnell, Mike, & Kuriyan, John. Crystal structure of a DNA polymerase sliding clamp from a Gram-positive bacterium. United States. https://doi.org/10.1186/1472-6807-6-2
Argiriadi, Maria A., Goedken, Eric R., Bruck, Irina, O'Donnell, Mike, and Kuriyan, John. Tue .
"Crystal structure of a DNA polymerase sliding clamp from a Gram-positive bacterium". United States. https://doi.org/10.1186/1472-6807-6-2. https://www.osti.gov/servlets/purl/1626550.
@article{osti_1626550,
title = {Crystal structure of a DNA polymerase sliding clamp from a Gram-positive bacterium},
author = {Argiriadi, Maria A. and Goedken, Eric R. and Bruck, Irina and O'Donnell, Mike and Kuriyan, John},
abstractNote = {Background: Sliding DNA clamps are processivity factors that are required for efficient DNA replication. DNA polymerases maintain proximity to nucleic acid templates by interacting with sliding clamps that encircle DNA and thereby link the polymerase enzyme to the DNA substrate. Although the structures of sliding clamps from Gram-negative bacteria (E. coli), eukaryotes, archaea, and T4-like bacteriophages are well-known, the structure of a sliding clamp from Grampositive bacteria has not been reported previously. Results: We have determined the crystal structure of the dimeric β subunit of the DNA polymerase III holoenzyme of Streptococcus pyogenes. The sliding clamp from this Gram-positive organism forms a ring-shaped dimeric assembly that is similar in overall structure to that of the sliding clamps from Gram-negative bacteria, bacteriophage T4, eukaryotes and archaea. The dimer has overall dimensions of ~90 Å × ~70 Å × ~25 Å with a central chamber that is large enough to accommodate duplex DNA. In comparison to the circular shape of other assemblies, the S. pyogenes clamp adopts a more elliptical structure. Conclusion: The sequences of sliding clamps from S. pyogenes and E. coli are only 23% identical, making the generation of structural models for the S. pyogenes clamp difficult in the absence of direct experimental information. Our structure of the S. pyogenes β subunit completes the catalog of clamp structures from all the major sequence grouping of sliding clamps. The more elliptical rather than circular structure of the S. pyogenes clamp implies that the topological nature of encircling DNA, rather than a precise geometric shape, is the most conserved aspect for this family of proteins.},
doi = {10.1186/1472-6807-6-2},
journal = {BMC Structural Biology (Online)},
number = 1,
volume = 6,
place = {United States},
year = {Tue Jan 10 00:00:00 EST 2006},
month = {Tue Jan 10 00:00:00 EST 2006}
}
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