DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila

Sotillos, Sol; Díaz-Meco, María Teresa; Caminero, Eva; Moscat, Jorge; Campuzano, Sonsoles

doi:10.1083/jcb.200311031

Title: DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila

Abstract

Both in Drosophila and vertebrate epithelial cells, the establishment of apicobasal polarity requires the apically localized, membrane-associated Par-3–Par-6–aPKC protein complex. In Drosophila, this complex colocalizes with the Crumbs–Stardust (Sdt)–Pals1-associated TJ protein (Patj) complex. Genetic and molecular analyses suggest a functional relationship between them. In this work we show, by overexpression of a kinase-dead Drosophila atypical PKC (DaPKC), the requirement for the kinase activity of DaPKC to maintain the position of apical determinants and to restrict the localization of basolateral ones. We demonstrate a novel physical interaction between the apical complexes, via direct binding of DaPKC to both Crb and Patj, and identify Crumbs as a phosphorylation target of DaPKC. This phosphorylation of Crumbs is functionally significant. Thus, a nonphosphorylatable Crumbs protein behaves in vivo as a dominant negative. Moreover, the phenotypic effect of overexpressing wild-type Crumbs is suppressed by reducing DaPKC activity. These results provide a mechanistic framework for the functional interaction between the Par-3–Par-6–aPKC and Crumbs–Sdt–Patj complexes based in the posttranslational modification of Crb by DaPKC.

Authors:

Sotillos, Sol ^[1]; Díaz-Meco, María Teresa ^[1]; Caminero, Eva ^[1]; Moscat, Jorge ^[1]; Campuzano, Sonsoles ^[1]

Consejo Superior de Investigaciones Científicas, Madrid (Spain). Centro de Biología Molecular Severo Ochoa; Universidad Autonóma de Madrid, Cantoblanco, Madrid (Spain)

Publication Date:: Mon Aug 09 00:00:00 EDT 2004

Research Org.:: Consejo Superior de Investigaciones Cientificas (CSIC), Madrid (Spain); Universidad Autonóma de Madrid, Cantoblanco, Madrid (Spain)

Sponsoring Org.:: Comunidad Autónoma de Madrid (CAM); Dirección General de Investigación Científica y Técnica; Fundación Ramón Areces

OSTI Identifier:: 1625133

Grant/Contract Number:: 08.5/0030/2000; PB98-0682; BMC2002-00411; SAF1999-0053; 2FD97-1429; SAF2000-0175; 08.1/0060/2000

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Cell Biology

Additional Journal Information:: Journal Volume: 166; Journal Issue: 4; Journal ID: ISSN 0021-9525

Publisher:: Rockefeller University Press

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; cell biology; DaPKC; Crumbs; Drosophila; epithelial cell polarity; signal transduction

Citation Formats


                    Sotillos, Sol, Díaz-Meco, María Teresa, Caminero, Eva, Moscat, Jorge, and Campuzano, Sonsoles. DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila.  United States: N. p., 2004. 
Web.  doi:10.1083/jcb.200311031.

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                    Sotillos, Sol, Díaz-Meco, María Teresa, Caminero, Eva, Moscat, Jorge, & Campuzano, Sonsoles. DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila.  United States.  https://doi.org/10.1083/jcb.200311031

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                    Sotillos, Sol, Díaz-Meco, María Teresa, Caminero, Eva, Moscat, Jorge, and Campuzano, Sonsoles. Mon .  
"DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila".  United States.  https://doi.org/10.1083/jcb.200311031.  https://www.osti.gov/servlets/purl/1625133.

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@article{osti_1625133,

  title        = {DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila},

  author       = {Sotillos, Sol and Díaz-Meco, María Teresa and Caminero, Eva and Moscat, Jorge and Campuzano, Sonsoles},

  abstractNote = {Both in Drosophila and vertebrate epithelial cells, the establishment of apicobasal polarity requires the apically localized, membrane-associated Par-3–Par-6–aPKC protein complex. In Drosophila, this complex colocalizes with the Crumbs–Stardust (Sdt)–Pals1-associated TJ protein (Patj) complex. Genetic and molecular analyses suggest a functional relationship between them. In this work we show, by overexpression of a kinase-dead Drosophila atypical PKC (DaPKC), the requirement for the kinase activity of DaPKC to maintain the position of apical determinants and to restrict the localization of basolateral ones. We demonstrate a novel physical interaction between the apical complexes, via direct binding of DaPKC to both Crb and Patj, and identify Crumbs as a phosphorylation target of DaPKC. This phosphorylation of Crumbs is functionally significant. Thus, a nonphosphorylatable Crumbs protein behaves in vivo as a dominant negative. Moreover, the phenotypic effect of overexpressing wild-type Crumbs is suppressed by reducing DaPKC activity. These results provide a mechanistic framework for the functional interaction between the Par-3–Par-6–aPKC and Crumbs–Sdt–Patj complexes based in the posttranslational modification of Crb by DaPKC.},

  doi          = {10.1083/jcb.200311031},

  journal      = {Journal of Cell Biology},

  number       = 4,

  volume       = 166,

  place        = {United States},

  year         = {Mon Aug 09 00:00:00 EDT 2004},

  month        = {Mon Aug 09 00:00:00 EDT 2004}

}

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Works referenced in this record:

Assembly of Epithelial Tight Junctions Is Negatively Regulated by Par6
journal, February 2002

Gao, Lin; Joberty, Gérard; Macara, Ian G.
Current Biology, Vol. 12, Issue 3
DOI: 10.1016/S0960-9822(01)00663-7

The discs-large tumor suppressor gene of Drosophila encodes a guanylate kinase homolog localized at septate junctions
journal, August 1991

Woods, Daniel F.; Bryant, Peter J.
Cell, Vol. 66, Issue 3
DOI: 10.1016/0092-8674(81)90009-X

An Atypical PKC Directly Associates and Colocalizes at the Epithelial Tight Junction with ASIP, a Mammalian Homologue of Caenorhabditis elegans Polarity Protein PAR-3
journal, October 1998

Izumi, Yasushi; Hirose, Tomonori; Tamai, Yoko
Journal of Cell Biology, Vol. 143, Issue 1
DOI: 10.1083/jcb.143.1.95

Crumbs interacts with moesin and βHeavy-spectrin in the apical membrane skeleton of Drosophila
journal, September 2002

Médina, Emmanuelle; Williams, Janice; Klipfell, Elizabeth
Journal of Cell Biology, Vol. 158, Issue 5
DOI: 10.1083/jcb.200203080

Drosophila PAR-1 and 14-3-3 Inhibit Bazooka/PAR-3 to Establish Complementary Cortical Domains in Polarized Cells
journal, December 2003

Benton, Richard; Johnston, Daniel St
Cell, Vol. 115, Issue 6
DOI: 10.1016/S0092-8674(03)00938-3

Epithelial Cell Polarity and Cell Junctions in Drosophila
journal, December 2001

Tepass, Ulrich; Tanentzapf, Guy; Ward, Robert
Annual Review of Genetics, Vol. 35, Issue 1
DOI: 10.1146/annurev.genet.35.102401.091415

Direct interaction of two polarity complexes implicated in epithelial tight junction assembly
journal, January 2003

Hurd, Toby W.; Gao, Lin; Roh, Michael H.
Nature Cell Biology, Vol. 5, Issue 2
DOI: 10.1038/ncb923

CRB3 Binds Directly to Par6 and Regulates the Morphogenesis of the Tight Junctions in Mammalian Epithelial Cells
journal, March 2004

Lemmers, Céline; Michel, Didier; Lane-Guermonprez, Lydie
Molecular Biology of the Cell, Vol. 15, Issue 3
DOI: 10.1091/mbc.e03-04-0235

Interaction of Par-6 and Crumbs complexes is essential for photoreceptor morphogenesis in Drosophila
journal, September 2003

Nam, S. -C.
Development, Vol. 130, Issue 18
DOI: 10.1242/dev.00648

armadillo, bazooka, and stardust are critical for early stages in formation of the zonula adherens and maintenance of the polarized blastoderm epithelium in Drosophila.
journal, July 1996

Müller, H. A.; Wieschaus, E.
Journal of Cell Biology, Vol. 134, Issue 1
DOI: 10.1083/jcb.134.1.149

Discs Lost, a Novel Multi-PDZ Domain Protein, Establishes and Maintains Epithelial Polarity
journal, March 1999

Bhat, Manzoor A.; Izaddoost, Shayan; Lu, Yue
Cell, Vol. 96, Issue 6
DOI: 10.1016/S0092-8674(00)80593-0

aPKC kinase activity is required for the asymmetric differentiation of the premature junctional complex during epithelial cell polarization
journal, September 2002

Suzuki, Atsushi; Ishiyama, Chikako; Hashiba, Katsutaka
Journal of Cell Science, Vol. 115, Issue 18
DOI: 10.1242/jcs.00032

Atypical PKC Phosphorylates PAR-1 Kinases to Regulate Localization and Activity
journal, April 2004

Hurov, Jonathan B.; Watkins, Janis L.; Piwnica-Worms, Helen
Current Biology, Vol. 14, Issue 8
DOI: 10.1016/j.cub.2004.04.007

Drosophila Atypical Protein Kinase C Associates with Bazooka and Controls Polarity of Epithelia and Neuroblasts
journal, September 2000

Wodarz, Andreas; Ramrath, Andreas; Grimm, Alexandra
Journal of Cell Biology, Vol. 150, Issue 6
DOI: 10.1083/jcb.150.6.1361

Localization of apical epithelial determinants by the basolateral PDZ protein Scribble
journal, February 2000

Bilder, David; Perrimon, Norbert
Nature, Vol. 403, Issue 6770
DOI: 10.1038/35001108

Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia
journal, December 2003

Rolls, Melissa M.; Albertson, Roger; Shih, Hsin-Pei
Journal of Cell Biology, Vol. 163, Issue 5
DOI: 10.1083/jcb.200306079

DmPAR-6 directs epithelial polarity and asymmetric cell division of neuroblasts in Drosophila
journal, December 2000

Petronczki, Mark; Knoblich, Juergen A.
Nature Cell Biology, Vol. 3, Issue 1
DOI: 10.1038/35050550

The Par complex directs asymmetric cell division by phosphorylating the cytoskeletal protein Lgl
journal, March 2003

Betschinger, Jörg; Mechtler, Karl; Knoblich, Juergen A.
Nature, Vol. 422, Issue 6929
DOI: 10.1038/nature01486

Apical, Lateral, and Basal Polarization Cues Contribute to the Development of the Follicular Epithelium during Drosophila Oogenesis
journal, November 2000

Tanentzapf, Guy; Smith, Christian; McGlade, Jane
Journal of Cell Biology, Vol. 151, Issue 4
DOI: 10.1083/jcb.151.4.891

Composition and Formation of Intercellular Junctions in Epithelial Cells
journal, December 2002

Knust, E.
Science, Vol. 298, Issue 5600
DOI: 10.1126/science.1072161

Integrated activity of PDZ protein complexes regulates epithelial polarity
journal, December 2002

Bilder, David; Schober, Markus; Perrimon, Norbert
Nature Cell Biology, Vol. 5, Issue 1
DOI: 10.1038/ncb897

Cooperative Regulation of Cell Polarity and Growth by Drosophila Tumor Suppressors
journal, July 2000

Bilder, D.
Science, Vol. 289, Issue 5476
DOI: 10.1126/science.289.5476.113

Visualization of Gene Expression in Living Adult Drosophila
journal, October 1996

Calleja, M.; Moreno, E.; Pelaz, S.
Science, Vol. 274, Issue 5285
DOI: 10.1126/science.274.5285.252

The Crumbs3-Pals1 complex participates in the establishment of polarity in mammalian epithelial cells
journal, July 2003

Roh, Michael H.; Fan, Shuling; Liu, Chia-Jen
Journal of Cell Science, Vol. 116, Issue 14
DOI: 10.1242/jcs.00500

Molecular cloning of lethal(2)giant larvae, a recessive oncogene of Drosophila melanogaster.
journal, June 1985

Mechler, B. M.; McGinnis, W.; Gehring, W. J.
The EMBO Journal, Vol. 4, Issue 6
DOI: 10.1002/j.1460-2075.1985.tb03816.x

Crumbs, a Component of the Apical Membrane, Is Required for Zonula Adherens Formation in Primary Epithelia ofDrosophila
journal, July 1996

Tepass, Ulrich
Developmental Biology, Vol. 177, Issue 1
DOI: 10.1006/dbio.1996.0157

Interactions between the crumbs, lethal giant larvae and bazooka pathways in epithelial polarization
journal, December 2002

Tanentzapf, Guy; Tepass, Ulrich
Nature Cell Biology, Vol. 5, Issue 1
DOI: 10.1038/ncb896

Drosophila Stardust interacts with Crumbs to control polarity of epithelia but not neuroblasts
journal, December 2001

Hong, Yang; Stronach, Beth; Perrimon, Norbert
Nature, Vol. 414, Issue 6864
DOI: 10.1038/414634a

Origins of Cell Polarity
journal, February 1996

Drubin, David G.; Nelson, W. James
Cell, Vol. 84, Issue 3
DOI: 10.1016/S0092-8674(00)81278-7

Zonula Adherens Formation in Caenorhabditis elegans Requires dlg-1, the Homologue of the Drosophila Gene discs large
journal, February 2001

Bossinger, Olaf; Klebes, Ansgar; Segbert, Christoph
Developmental Biology, Vol. 230, Issue 1
DOI: 10.1006/dbio.2000.0113

Drosophila Stardust is a partner of Crumbs in the control of epithelial cell polarity
journal, December 2001

Bachmann, André; Schneider, Martina; Theilenberg, Eva
Nature, Vol. 414, Issue 6864
DOI: 10.1038/414638a

Composition and function of PDZ protein complexes during cell polarization
journal, September 2003

Roh, Michael H.; Margolis, Ben
American Journal of Physiology-Renal Physiology, Vol. 285, Issue 3
DOI: 10.1152/ajprenal.00086.2003

Loss of PALS1 Expression Leads to Tight Junction and Polarity Defects
journal, April 2004

Straight, Samuel W.; Shin, Kunyoo; Fogg, Vanessa C.
Molecular Biology of the Cell, Vol. 15, Issue 4
DOI: 10.1091/mbc.e03-08-0620

Control of spindle orientation in Drosophila by the Par-3-related PDZ-domain protein Bazooka
journal, December 1998

Kuchinke, Ute; Grawe, Ferdi; Knust, Elisabeth
Current Biology, Vol. 8, Issue 25
DOI: 10.1016/S0960-9822(98)00016-5

A polarity complex of mPar-6 and atypical PKC binds, phosphorylates and regulates mammalian Lgl
journal, March 2003

Plant, Pamela J.; Fawcett, James P.; Lin, Dan C. C.
Nature Cell Biology, Vol. 5, Issue 4
DOI: 10.1038/ncb948

The Maguk protein, Pals1, functions as an adapter, linking mammalian homologues of Crumbs and Discs Lost
journal, April 2002

Roh, Michael H.; Makarova, Olga; Liu, Chia-Jen
Journal of Cell Biology, Vol. 157, Issue 1
DOI: 10.1083/jcb.200109010

The interaction of p62 with RIP links the atypical PKCs to NF-kappa B activation
journal, June 1999

Sanz, L.
The EMBO Journal, Vol. 18, Issue 11
DOI: 10.1093/emboj/18.11.3044

The Drosophila Cell Survival Gene discs lost Encodes a Cytoplasmic Codanin-1-like Protein, Not a Homolog of Tight Junction PDZ Protein Patj
journal, December 2003

Pielage, Jan; Stork, Tobias; Bunse, Ingrid
Developmental Cell, Vol. 5, Issue 6
DOI: 10.1016/S1534-5807(03)00358-7

Genetic control of epithelial cell polarity: Lessons fromDrosophila
journal, May 2000

M�ller, H. -Arno J.
Developmental Dynamics, Vol. 218, Issue 1
DOI: 10.1002/(SICI)1097-0177(200005)218:1<52::AID-DVDY5>3.0.CO;2-L

Atypical Protein Kinase C Is Involved in the Evolutionarily Conserved Par Protein Complex and Plays a Critical Role in Establishing Epithelia-Specific Junctional Structures
journal, March 2001

Suzuki, Atsushi; Yamanaka, Tomoyuki; Hirose, Tomonori
Journal of Cell Biology, Vol. 152, Issue 6
DOI: 10.1083/jcb.152.6.1183

Expression of crumbs confers apical character on plasma membrane domains of ectodermal epithelia of drosophila
journal, July 1995

Wodarz, Andreas; Hinz, Uwe; Engelbert, Martin
Cell, Vol. 82, Issue 1
DOI: 10.1016/0092-8674(95)90053-5

Parsing the Polarity Code
journal, March 2004

Macara, Ian G.
Nature Reviews Molecular Cell Biology, Vol. 5, Issue 3
DOI: 10.1038/nrm1332

Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
journal, June 1993

Brand, A. H.; Perrimon, N.
Development, Vol. 118, Issue 2
DOI: 10.1242/dev.118.2.401

The Drosophila genes crumbs and stardust are involved in the biogenesis of adherens junctions
journal, March 1996

Grawe, F.; Wodarz, A.; Lee, B.
Development, Vol. 122, Issue 3
DOI: 10.1242/dev.122.3.951

Involvement of ASIP/PAR-3 in the promotion of epithelial tight junction formation
journal, June 2002

Hirose, Tomonori; Izumi, Yasushi; Nagashima, Yoji
Journal of Cell Science, Vol. 115, Issue 12
DOI: 10.1242/jcs.115.12.2485

Drosophila neurotactin, a surface glycoprotein with homology to serine esterases, is dynamically expressed during embryogenesis
journal, December 1990

Hortsch, M.; Patel, N. H.; Bieber, A. J.
Development, Vol. 110, Issue 4
DOI: 10.1242/dev.110.4.1327

Expression of crumbs confers apical character on plasma membrane domains of ectodermal epithelia of Drosophila
journal, October 1995

Wodarz, A.; Hinz, U.; Engelbert, M.
Trends in Genetics, Vol. 11, Issue 10, p. 387
DOI: 10.1016/s0168-9525(00)89127-1

Discs Lost, a Novel Multi-PDZ Domain Protein, Establishes and Maintains Epithelial Polarity
journal, December 2003

Bhat, Manzoor A.; Izaddoost, Shayan; Lu, Yue
Cell, Vol. 115, Issue 6
DOI: 10.1016/s0092-8674(03)00973-5

Molecular cloning of lethal(2)giant larvae, a recessive oncogene of Drosophila melanogaster.
journal, June 1985

Mechler, B. M.; McGinnis, W.; Gehring, W. J.
The EMBO Journal, Vol. 4, Issue 6
DOI: 10.1002/j.1460-2075.1985.tb03816.x

Protein kinase C ζ isoform is critical for mitogenic signal transduction
journal, August 1993

Berra, Edurne; Diaz-Meco, Maria T.; Dominguez, Isabel
Cell, Vol. 74, Issue 3
DOI: 10.1016/0092-8674(93)80056-k

Intercellular junctions and cellular polarity: the PAR–aPKC complex, a conserved core cassette playing fundamental roles in cell polarity
journal, October 2001

Ohno, Shigeo
Current Opinion in Cell Biology, Vol. 13, Issue 5
DOI: 10.1016/s0955-0674(00)00264-7

Positional cloning of heart and soul reveals multiple roles for PKCλ in zebrafish organogenesis
journal, October 2001

Horne-Badovinac, Sally; Lin, Dan; Waldron, Steve
Current Biology, Vol. 11, Issue 19
DOI: 10.1016/s0960-9822(01)00458-4

A conserved motif in Crumbs is required for E-cadherin localisation and zonula adherens formation in Drosophila
journal, January 2000

Klebes, Ansgar; Knust, Elisabeth
Current Biology, Vol. 10, Issue 2
DOI: 10.1016/s0960-9822(99)00277-8

Targeted Disruption of the ζPKC Gene Results in the Impairment of the NF-κB Pathway
journal, October 2001

Leitges, Michael; Sanz, Laura; Martin, Pilar
Molecular Cell, Vol. 8, Issue 4
DOI: 10.1016/s1097-2765(01)00361-6

Cell polarity: the PARty expands
journal, January 2001

Doe, Chris Q.
Nature Cell Biology, Vol. 3, Issue 1
DOI: 10.1038/35050684

Drosophila Stardust interacts with Crumbs to control polarity of epithelia but not neuroblasts
journal, December 2001

Hong, Yang; Stronach, Beth; Perrimon, Norbert
Nature, Vol. 414, Issue 6864
DOI: 10.1038/414634a

Drosophila Stardust is a partner of Crumbs in the control of epithelial cell polarity
journal, December 2001

Bachmann, André; Schneider, Martina; Theilenberg, Eva
Nature, Vol. 414, Issue 6864
DOI: 10.1038/414638a

The Par complex directs asymmetric cell division by phosphorylating the cytoskeletal protein Lgl
journal, March 2003

Betschinger, Jörg; Mechtler, Karl; Knoblich, Juergen A.
Nature, Vol. 422, Issue 6929
DOI: 10.1038/nature01486

Parsing the Polarity Code
journal, March 2004

Macara, Ian G.
Nature Reviews Molecular Cell Biology, Vol. 5, Issue 3
DOI: 10.1038/nrm1332

Drosophila Atypical Protein Kinase C Associates with Bazooka and Controls Polarity of Epithelia and Neuroblasts
journal, September 2000

Wodarz, Andreas; Ramrath, Andreas; Grimm, Alexandra
Journal of Cell Biology, Vol. 150, Issue 6
DOI: 10.1083/jcb.150.6.1361

Atypical Protein Kinase C Is Involved in the Evolutionarily Conserved Par Protein Complex and Plays a Critical Role in Establishing Epithelia-Specific Junctional Structures
journal, March 2001

Suzuki, Atsushi; Yamanaka, Tomoyuki; Hirose, Tomonori
Journal of Cell Biology, Vol. 152, Issue 6
DOI: 10.1083/jcb.152.6.1183

Composition and Formation of Intercellular Junctions in Epithelial Cells
journal, December 2002

Knust, E.
Science, Vol. 298, Issue 5600
DOI: 10.1126/science.1072161

Epithelial Cell Polarity and Cell Junctions in Drosophila
journal, December 2001

Tepass, Ulrich; Tanentzapf, Guy; Ward, Robert
Annual Review of Genetics, Vol. 35, Issue 1
DOI: 10.1146/annurev.genet.35.102401.091415

Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
journal, June 1993

Brand, A. H.; Perrimon, N.
Development, Vol. 118, Issue 2
DOI: 10.1242/dev.118.2.401

The Drosophila genes crumbs and stardust are involved in the biogenesis of adherens junctions
journal, March 1996

Grawe, F.; Wodarz, A.; Lee, B.
Development, Vol. 122, Issue 3
DOI: 10.1242/dev.122.3.951

The Crumbs3-Pals1 complex participates in the establishment of polarity in mammalian epithelial cells
journal, July 2003

Roh, Michael H.; Fan, Shuling; Liu, Chia-Jen
Journal of Cell Science, Vol. 116, Issue 14
DOI: 10.1242/jcs.00500

Involvement of ASIP/PAR-3 in the promotion of epithelial tight junction formation
journal, June 2002

Hirose, Tomonori; Izumi, Yasushi; Nagashima, Yoji
Journal of Cell Science, Vol. 115, Issue 12
DOI: 10.1242/jcs.115.12.2485

Works referencing / citing this record:

The Hippo pathway and apico–basal cell polarity
journal, May 2011

Genevet, Alice; Tapon, Nicolas
Biochemical Journal, Vol. 436, Issue 2
DOI: 10.1042/bj20110217

Coordinated protein sorting, targeting and distribution in polarized cells
journal, November 2008

Mellman, Ira; Nelson, W. James
Nature Reviews Molecular Cell Biology, Vol. 9, Issue 11
DOI: 10.1038/nrm2525

Atypical PKC contributes to poor prognosis through loss of apical-basal polarity and Cyclin E overexpression in ovarian cancer
journal, August 2005

Eder, A. M.; Sui, X.; Rosen, D. G.
Proceedings of the National Academy of Sciences, Vol. 102, Issue 35
DOI: 10.1073/pnas.0505641102

Using Drosophila melanogaster to map human cancer pathways
journal, July 2005

Brumby, Anthony M.; Richardson, Helena E.
Nature Reviews Cancer, Vol. 5, Issue 8
DOI: 10.1038/nrc1671

Crumbs3 Is Essential for Proper Epithelial Development and Viability
journal, January 2014

Whiteman, Eileen L.; Fan, Shuling; Harder, Jennifer L.
Molecular and Cellular Biology, Vol. 34, Issue 1
DOI: 10.1128/mcb.00999-13

Yorkie and JNK revert syncytial muscles into myoblasts during Org-1–dependent lineage reprogramming
journal, October 2019

Schaub, Christoph; Rose, Marcel; Frasch, Manfred
Journal of Cell Biology, Vol. 218, Issue 11
DOI: 10.1083/jcb.201905048

Polarization and Myelination in Myelinating Glia
journal, December 2012

Masaki, Toshihiro
ISRN Neurology, Vol. 2012
DOI: 10.5402/2012/769412

Multiple domains in the Crumbs Homolog 2a (Crb2a) protein are required for regulating rod photoreceptor size
journal, July 2010

Hsu, Ya-Chu; Jensen, Abbie M.
BMC Cell Biology, Vol. 11, Issue 1
DOI: 10.1186/1471-2121-11-60

A Polybasic Domain in aPKC Mediates Par-6-Dependent Control of Plasma Membrane Targeting and Kinase Activity
posted_content, January 2019

Dong, Wei; Lu, Juan; Zhang, Xuejing
DOI: 10.1101/588624

Expression and localization of the polarity protein CRB2 in adult mouse brain: a comparison with the CRB1rd8 mutant mouse model
journal, August 2018

Dolón, Jorge F.; Paniagua, Antonio E.; Valle, Vicente
Scientific Reports, Vol. 8, Issue 1
DOI: 10.1038/s41598-018-30210-5

Towards understanding CRUMBS function in retinal dystrophies
journal, October 2006

Richard, Mélisande; Roepman, Ronald; Aartsen, Wendy M.
Human Molecular Genetics, Vol. 15, Issue suppl_2
DOI: 10.1093/hmg/ddl195

Nuclear fallout provides a new link between aPKC and polarized cell trafficking
journal, April 2016

Calero-Cuenca, Francisco J.; Espinosa-Vázquez, José Manuel; Reina-Campos, Miguel
BMC Biology, Vol. 14, Issue 1
DOI: 10.1186/s12915-016-0253-6

Epithelial cell polarity: what flies can teach us about cancer
journal, August 2012

Bergstralh, Daniel T.; St Johnston, Daniel
Essays in Biochemistry, Vol. 53
DOI: 10.1042/bse0530129

Mesenchymal–epithelial transition in development and reprogramming
journal, January 2019

Pei, Duanqing; Shu, Xiaodong; Gassama-Diagne, Ama
Nature Cell Biology, Vol. 21, Issue 1
DOI: 10.1038/s41556-018-0195-z

Polarity Protein Complex Scribble/Lgl/Dlg And Epithelial Cell Barriers
book, January 2013

Su, Wen-Hui; Mruk, Dolores D.; Wong, Elissa W. P.
Advances in Experimental Medicine and Biology
DOI: 10.1007/978-1-4614-4711-5_7

Epithelial cell polarity, stem cells and cancer
journal, December 2011

Martin-Belmonte, Fernando; Perez-Moreno, Mirna
Nature Reviews Cancer, Vol. 12, Issue 1
DOI: 10.1038/nrc3169

Trafficking through COPII Stabilises Cell Polarity and Drives Secretion during Drosophila Epidermal Differentiation
journal, May 2010

Norum, Michaela; Tång, Erika; Chavoshi, Tina
PLoS ONE, Vol. 5, Issue 5
DOI: 10.1371/journal.pone.0010802

Evolution and Cell Physiology. 4. Why invent yet another protein complex to build junctions in epithelial cells?
journal, December 2013

Bivic, André Le
American Journal of Physiology-Cell Physiology, Vol. 305, Issue 12
DOI: 10.1152/ajpcell.00272.2013

Domain-specific functions of Stardust in Drosophila embryonic development
journal, November 2016

Koch, Leonie; Feicht, Sabine; Sun, Rui
Royal Society Open Science, Vol. 3, Issue 11
DOI: 10.1098/rsos.160776

Apical transport of Crumbs maintains epithelial cell polarity
posted_content, January 2019

Aguilar-Aragon, M.; Fletcher, G.; Thompson, B. J.
bioRxiv
DOI: 10.1101/592311

Disruption of Rab8a and Rab11a causes formation of basolateral microvilli in neonatal enteropathy
journal, January 2017

Feng, Qiang; Bonder, Edward M.; Engevik, Amy C.
Journal of Cell Science
DOI: 10.1242/jcs.201897

Atypical protein kinase C induces cell transformation by disrupting Hippo/Yap signaling
journal, October 2015

Archibald, Andrew; Al-Masri, Maia; Liew-Spilger, Alyson
Molecular Biology of the Cell, Vol. 26, Issue 20
DOI: 10.1091/mbc.e15-05-0265

Drosophila Models of Cell Polarity and Cell Competition in Tumourigenesis
book, January 2019

Fahey-Lozano, Natasha; La Marca, John E.; Portela, Marta
Advances in Experimental Medicine and Biology
DOI: 10.1007/978-3-030-23629-8_3

The cytoskeleton and cancer
journal, January 2009

Hall, Alan
Cancer and Metastasis Reviews, Vol. 28, Issue 1-2
DOI: 10.1007/s10555-008-9166-3

The Crumbs3 Polarity Protein
journal, March 2017

Margolis, Ben
Cold Spring Harbor Perspectives in Biology, Vol. 10, Issue 3
DOI: 10.1101/cshperspect.a027961

α-Tocopherol promotes HaCaT keratinocyte wound repair through the regulation of polarity proteins leading to the polarized cell migration: α-Tocopherol Promotes HaCaT Keratinocyte Wound Repair
journal, February 2018

Horikoshi, Yosuke; Kamizaki, Kouki; Hanaki, Takehiko
BioFactors, Vol. 44, Issue 2
DOI: 10.1002/biof.1414

PAR3–PAR6–atypical PKC polarity complex proteins in neuronal polarization
journal, April 2018

Hapak, Sophie M.; Rothlin, Carla V.; Ghosh, Sourav
Cellular and Molecular Life Sciences, Vol. 75, Issue 15
DOI: 10.1007/s00018-018-2828-6

Rho-kinase planar polarisation at tissue boundaries depends on phospho-regulation of membrane residence time
posted_content, January 2019

Sidor, Clara; Stevens, Tim J.; Jin, Li
DOI: 10.1101/615062

The apical-basal cell polarity determinant Crumbs regulates Hippo signaling in Drosophila
journal, August 2010

Chen, C. -L.; Gajewski, K. M.; Hamaratoglu, F.
Proceedings of the National Academy of Sciences, Vol. 107, Issue 36
DOI: 10.1073/pnas.1004060107

Formation of a Bazooka–Stardust complex is essential for plasma membrane polarity in epithelia
journal, September 2010

Krahn, Michael P.; Bückers, Johanna; Kastrup, Lars
Journal of Cell Biology, Vol. 190, Issue 5
DOI: 10.1083/jcb.201006029

A conserved polybasic domain mediates plasma membrane targeting of Lgl and its regulation by hypoxia
journal, October 2015

Dong, Wei; Zhang, Xuejing; Liu, Weijie
The Journal of Cell Biology, Vol. 211, Issue 2
DOI: 10.1083/jcb.201503067

Tissue Polarity-Dependent Control of Mammary Epithelial Homeostasis and Cancer Development: an Epigenetic Perspective
journal, January 2010

Lelièvre, Sophie A.
Journal of Mammary Gland Biology and Neoplasia, Vol. 15, Issue 1
DOI: 10.1007/s10911-010-9168-y

Neural stem cell temporal patterning and brain tumour growth rely on oxidative phosphorylation
journal, September 2019

van den Ameele, Jelle; Brand, Andrea H.
eLife, Vol. 8
DOI: 10.7554/elife.47887

A dual role of the extracellular domain of Drosophila Crumbs for morphogenesis of the embryonic neuroectoderm
journal, January 2018

Das, Shradha; Knust, Elisabeth
Biology Open, Vol. 7, Issue 1
DOI: 10.1242/bio.031435

Casein kinase 1 family proteins promote Slimb-dependent Expanded degradation
journal, September 2019

Fulford, Alexander D.; Holder, Maxine V.; Frith, David
eLife, Vol. 8
DOI: 10.7554/elife.46592

Control of tumourigenesis by the Scribble/Dlg/Lgl polarity module
journal, November 2008

Humbert, P. O.; Grzeschik, N. A.; Brumby, A. M.
Oncogene, Vol. 27, Issue 55
DOI: 10.1038/onc.2008.341

Localization and Function of Pals1-associated Tight Junction Protein in Drosophila Is Regulated by Two Distinct Apical Complexes
journal, May 2015

Sen, Arnab; Sun, Rui; Krahn, Michael P.
Journal of Biological Chemistry, Vol. 290, Issue 21
DOI: 10.1074/jbc.m114.629014

Organogenesis and tumorigenesis: Insight from the JAK/STAT pathway in the Drosophila eye
journal, August 2010

Wang, Ying-Hsuan; Huang, Min-Lang
Developmental Dynamics, Vol. 239, Issue 10
DOI: 10.1002/dvdy.22394

Cdc42 and Par proteins stabilize dynamic adherens junctions in the Drosophila neuroectoderm through regulation of apical endocytosis
journal, December 2008

Harris, Kathryn P.; Tepass, Ulrich
Journal of Cell Biology, Vol. 183, Issue 6
DOI: 10.1083/jcb.200807020

A bidirectional antagonism between aPKC and Yurt regulates epithelial cell polarity
journal, February 2014

Gamblin, Clémence L.; Hardy, Émilie J. -L.; Chartier, François J. -M.
Journal of Cell Biology, Vol. 204, Issue 4
DOI: 10.1083/jcb.201308032

Atf3 links loss of epithelial polarity to defects in cell differentiation and cytoarchitecture
journal, March 2018

Donohoe, Colin D.; Csordás, Gábor; Correia, Andreia
PLOS Genetics, Vol. 14, Issue 3
DOI: 10.1371/journal.pgen.1007241

Novel Neuroprotective Function of Apical-Basal Polarity Gene Crumbs in Amyloid Beta 42 (Aβ42) Mediated Neurodegeneration
journal, November 2013

Steffensmeier, Andrew M.; Tare, Meghana; Puli, Oorvashi Roy
PLoS ONE, Vol. 8, Issue 11
DOI: 10.1371/journal.pone.0078717

Oligomerization of the FERM-FA protein Yurt controls epithelial cell polarity
journal, August 2018

Gamblin, Clémence L.; Parent-Prévost, Frédérique; Jacquet, Kévin
Journal of Cell Biology, Vol. 217, Issue 11
DOI: 10.1083/jcb.201803099

The positioning and segregation of apical cues during epithelial polarity establishment in Drosophila
journal, August 2005

Harris, Tony J. C.; Peifer, Mark
Journal of Cell Biology, Vol. 170, Issue 5
DOI: 10.1083/jcb.200505127

Enteropathogenic Escherichia coli (EPEC) Recruitment of PAR Polarity Protein Atypical PKCζ to Pedestals and Cell–Cell Contacts Precedes Disruption of Tight Junctions in Intestinal Epithelial Cells
journal, January 2020

Tapia, Rocio; Kralicek, Sarah E.; Hecht, Gail A.
International Journal of Molecular Sciences, Vol. 21, Issue 2
DOI: 10.3390/ijms21020527

Yorkie and JNK revert syncytial muscles into myoblasts during Org-1 dependent lineage reprogramming
posted_content, January 2019

Schaub, Christoph; Rose, Marcel; Frasch, Manfred
DOI: 10.1101/607820

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journal, June 2020

Dong, Wei; Lu, Juan; Zhang, Xuejing
Journal of Cell Biology, Vol. 219, Issue 7
DOI: 10.1083/jcb.201903031

The positioning and segregation of apical cues during epithelial polarity establishment in Drosophila
text, January 2005

J. C., Harris, Tony; Mark, Peifer,
The University of North Carolina at Chapel Hill University Libraries
DOI: 10.17615/fvst-yg23

Neural stem cell temporal patterning and brain tumour growth rely on oxidative phosphorylation.
text, January 2019

Van Den Ameele, Jelle; Brand, Andrea
Apollo - University of Cambridge Repository
DOI: 10.17863/cam.44301

Casein kinase 1 family proteins promote Slimb-dependent Expanded degradation.
text, January 2020

Fulford, Alexander D.; Holder, Maxine V.; Frith, David
The Francis Crick Institute
DOI: 10.25418/crick.11637006.v1

Rho-Kinase Planar Polarization at Tissue Boundaries Depends on Phospho-regulation of Membrane Residence Time
journal, February 2020

Sidor, Clara; Stevens, Tim J.; Jin, Li
Developmental Cell, Vol. 52, Issue 3
DOI: 10.1016/j.devcel.2019.12.003

Atf3 links loss of epithelial polarity to defects in cell differentiation and cytoarchitecture
posted_content, February 2018

Donohoe, Colin D.; Csordas, Gabor; Correia, Andreia
PLOS Genetics
DOI: 10.1101/212969

Drosophila models of cell polarity and cell competition in tumourigenesis
other, January 2022

Fahey-Lozano, Natalia; La-Marca, John; Portela-Esteban, Marta
La Trobe
DOI: 10.26181/19305878.v3

Tight junction and polarity interaction in the transporting epithelial phenotype
journal, March 2008

Cereijido, Marcelino; Contreras, Rubén G.; Shoshani, Liora
Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1778, Issue 3
DOI: 10.1016/j.bbamem.2007.09.001

aPKC Phosphorylation of Bazooka Defines the Apical/Lateral Border in Drosophila Epithelial Cells
journal, April 2010

Morais-de-Sá, Eurico; Mirouse, Vincent; St Johnston, Daniel
Cell, Vol. 141, Issue 3
DOI: 10.1016/j.cell.2010.02.040

Coordinated Control of Cell Adhesion, Polarity, and Cytoskeleton Underlies Hox-Induced Organogenesis in Drosophila
journal, November 2006

Lovegrove, Bridget; Simões, Sergio; Rivas, María Luisa
Current Biology, Vol. 16, Issue 22
DOI: 10.1016/j.cub.2006.09.029

KIBRA Suppresses Apical Exocytosis through Inhibition of aPKC Kinase Activity in Epithelial Cells
journal, April 2011

Yoshihama, Yohei; Sasaki, Kazunori; Horikoshi, Yosuke
Current Biology, Vol. 21, Issue 8
DOI: 10.1016/j.cub.2011.03.029

Lgl Regulates Notch Signaling via Endocytosis, Independently of the Apical aPKC-Par6-Baz Polarity Complex
journal, September 2014

Parsons, Linda M.; Portela, Marta; Grzeschik, Nicola A.
Current Biology, Vol. 24, Issue 18
DOI: 10.1016/j.cub.2014.07.075

Fragile X Protein Functions with Lgl and the PAR Complex in Flies and Mice
journal, January 2005

Zarnescu, Daniela C.; Jin, Peng; Betschinger, Joerg
Developmental Cell, Vol. 8, Issue 1
DOI: 10.1016/j.devcel.2004.10.020

aPKC Controls Microtubule Organization to Balance Adherens Junction Symmetry and Planar Polarity during Development
journal, May 2007

Harris, Tony J. C.; Peifer, Mark
Developmental Cell, Vol. 12, Issue 5
DOI: 10.1016/j.devcel.2007.02.011

The Tumor Suppressors Brat and Numb Regulate Transit-Amplifying Neuroblast Lineages in Drosophila
journal, April 2008

Bowman, Sarah K.; Rolland, Vivien; Betschinger, Joerg
Developmental Cell, Vol. 14, Issue 4
DOI: 10.1016/j.devcel.2008.03.004

Anisotropy of Crumbs and aPKC Drives Myosin Cable Assembly during Tube Formation
journal, November 2012

Röper, Katja
Developmental Cell, Vol. 23, Issue 5
DOI: 10.1016/j.devcel.2012.09.013

Rho-Kinase Planar Polarization at Tissue Boundaries Depends on Phospho-regulation of Membrane Residence Time
journal, February 2020

Sidor, Clara; Stevens, Tim J.; Jin, Li
Developmental Cell, Vol. 52, Issue 3
DOI: 10.1016/j.devcel.2019.12.003

The cytoskeletal motor proteins Dynein and MyoV direct apical transport of Crumbs
journal, March 2020

Aguilar-Aragon, M.; Fletcher, G.; Thompson, B. J.
Developmental Biology, Vol. 459, Issue 2
DOI: 10.1016/j.ydbio.2019.12.009

Epithelial cell polarity: a major gatekeeper against cancer?
journal, May 2011

Royer, C.; Lu, X.
Cell Death & Differentiation, Vol. 18, Issue 9
DOI: 10.1038/cdd.2011.60

The positioning and segregation of apical cues during epithelial polarity establishment in Drosophila
journal, August 2005

Harris, Tony J. C.; Peifer, Mark
Journal of Cell Biology, Vol. 170, Issue 5
DOI: 10.1083/jcb.200505127

p32 is a novel mammalian Lgl binding protein that enhances the activity of protein kinase Cζ and regulates cell polarity
journal, August 2007

Bialucha, Carl U.; Ferber, Emma C.; Pichaud, Franck
The Journal of Cell Biology, Vol. 178, Issue 4
DOI: 10.1083/jcb.200612022

Formation of a Bazooka–Stardust complex is essential for plasma membrane polarity in epithelia
journal, September 2010

Krahn, Michael P.; Bückers, Johanna; Kastrup, Lars
Journal of Cell Biology, Vol. 190, Issue 5
DOI: 10.1083/jcb.201006029

Atypical protein kinase C induces cell transformation by disrupting Hippo/Yap signaling
journal, October 2015

Archibald, Andrew; Al-Masri, Maia; Liew-Spilger, Alyson
Molecular Biology of the Cell, Vol. 26, Issue 20
DOI: 10.1091/mbc.e15-05-0265

A Polybasic Domain in aPKC Mediates Par-6-Dependent Control of Plasma Membrane Targeting and Kinase Activity
posted_content, January 2019

Dong, Wei; Lu, Juan; Zhang, Xuejing
DOI: 10.1101/588624

Apical transport of Crumbs maintains epithelial cell polarity
posted_content, January 2019

Aguilar-Aragon, M.; Fletcher, G.; Thompson, B. J.
bioRxiv
DOI: 10.1101/592311

Tight Junctions: A Barrier to the Initiation and Progression of Breast Cancer?
journal, January 2010

Brennan, Kieran; Offiah, Gozie; McSherry, Elaine A.
Journal of Biomedicine and Biotechnology, Vol. 2010
DOI: 10.1155/2010/460607

Multiple domains in the Crumbs Homolog 2a (Crb2a) protein are required for regulating rod photoreceptor size
journal, July 2010

Hsu, Ya-Chu; Jensen, Abbie M.
BMC Cell Biology, Vol. 11, Issue 1
DOI: 10.1186/1471-2121-11-60

Loss of the Drosophila cell polarity regulator Scribbled promotes epithelial tissue overgrowth and cooperation with oncogenic Ras-Raf through impaired Hippo pathway signaling
journal, January 2011

Doggett, Karen; Grusche, Felix A.; Richardson, Helena E.
BMC Developmental Biology, Vol. 11, Issue 1
DOI: 10.1186/1471-213x-11-57

Nuclear fallout provides a new link between aPKC and polarized cell trafficking
journal, April 2016

Calero-Cuenca, Francisco J.; Espinosa-Vázquez, José Manuel; Reina-Campos, Miguel
BMC Biology, Vol. 14, Issue 1
DOI: 10.1186/s12915-016-0253-6

The BTB-zinc Finger Transcription Factor Abrupt Acts as an Epithelial Oncogene in Drosophila melanogaster through Maintaining a Progenitor-like Cell State
journal, July 2013

Turkel, Nezaket; Sahota, Virender K.; Bolden, Jessica E.
PLoS Genetics, Vol. 9, Issue 7
DOI: 10.1371/journal.pgen.1003627

Functional Interactions between the erupted/tsg101 Growth Suppressor Gene and the DaPKC and rbf1 Genes in Drosophila Imaginal Disc Tumors
journal, September 2009

Gilbert, M. Melissa; Robinson, Brian S.; Moberg, Kenneth H.
PLoS ONE, Vol. 4, Issue 9
DOI: 10.1371/journal.pone.0007039

A Modifier Screen for Bazooka/PAR-3 Interacting Genes in the Drosophila Embryo Epithelium
journal, April 2010

Shao, Wei; Wu, Johnny; Chen, Jeyla
PLoS ONE, Vol. 5, Issue 4
DOI: 10.1371/journal.pone.0009938

Genetic Interaction of Centrosomin and Bazooka in Apical Domain Regulation in Drosophila Photoreceptor
journal, January 2011

Chen, Geng; Rogers, Alicia K.; League, Garrett P.
PLoS ONE, Vol. 6, Issue 1
DOI: 10.1371/journal.pone.0016127

Protein Kinase C-Dependent Signaling Controls the Midgut Epithelial Barrier to Malaria Parasite Infection in Anopheline Mosquitoes
journal, October 2013

Pakpour, Nazzy; Camp, Lauren; Smithers, Hannah M.
PLoS ONE, Vol. 8, Issue 10
DOI: 10.1371/journal.pone.0076535

Intrinsic regulation of enteroendocrine fate by Numb
journal, May 2017

Sallé, Jérémy; Gervais, Louis; Boumard, Benjamin
The EMBO Journal, Vol. 36, Issue 13
DOI: 10.15252/embj.201695622

Neural stem cell temporal patterning and brain tumour growth rely on oxidative phosphorylation.
text, January 2019

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Apollo - University of Cambridge Repository
DOI: 10.17863/cam.44301

Casein kinase 1 family proteins promote Slimb-dependent Expanded degradation.
text, January 2020

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The Francis Crick Institute
DOI: 10.25418/crick.11637006

The Scribble Cell Polarity Module in the Regulation of Cell Signaling in Tissue Development and Tumorigenesis
text, January 2022

Stephens, Rebecca; Lim, Krystle; Portela-Esteban, Marta
La Trobe
DOI: 10.26181/19305938.v1

Polarization and Myelination in Myelinating Glia
journal, December 2012

Masaki, Toshihiro
ISRN Neurology, Vol. 2012
DOI: 10.5402/2012/769412

Drosophila: A Model System That Allows in vivo Manipulation and Study of Epithelial Cell Polarity
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Leibfried, Andrea; Bellaiche, Yohanns
Current Frontiers and Perspectives in Cell Biology
DOI: 10.5772/37120

Epithelial Cell Polarity Determinant CRB3 in Cancer Development
journal, January 2015

Li, Pingping; Mao, Xiaona; Ren, Yu
International Journal of Biological Sciences, Vol. 11, Issue 1
DOI: 10.7150/ijbs.10615

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Title: DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila

Abstract

Citation Formats

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An Atypical PKC Directly Associates and Colocalizes at the Epithelial Tight Junction with ASIP, a Mammalian Homologue of Caenorhabditis elegans Polarity Protein PAR-3 journal, October 1998

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Epithelial Cell Polarity and Cell Junctions in Drosophila journal, December 2001

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Molecular cloning of lethal(2)giant larvae, a recessive oncogene of Drosophila melanogaster. journal, June 1985

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Involvement of ASIP/PAR-3 in the promotion of epithelial tight junction formation journal, June 2002

Drosophila neurotactin, a surface glycoprotein with homology to serine esterases, is dynamically expressed during embryogenesis journal, December 1990

Expression of crumbs confers apical character on plasma membrane domains of ectodermal epithelia of Drosophila journal, October 1995

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Molecular cloning of lethal(2)giant larvae, a recessive oncogene of Drosophila melanogaster. journal, June 1985

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The Drosophila genes crumbs and stardust are involved in the biogenesis of adherens junctions journal, March 1996

The Crumbs3-Pals1 complex participates in the establishment of polarity in mammalian epithelial cells journal, July 2003

Involvement of ASIP/PAR-3 in the promotion of epithelial tight junction formation journal, June 2002

The Hippo pathway and apico–basal cell polarity journal, May 2011

Coordinated protein sorting, targeting and distribution in polarized cells journal, November 2008

Atypical PKC contributes to poor prognosis through loss of apical-basal polarity and Cyclin E overexpression in ovarian cancer journal, August 2005

Using Drosophila melanogaster to map human cancer pathways journal, July 2005

Crumbs3 Is Essential for Proper Epithelial Development and Viability journal, January 2014

Yorkie and JNK revert syncytial muscles into myoblasts during Org-1–dependent lineage reprogramming journal, October 2019

Polarization and Myelination in Myelinating Glia journal, December 2012

Multiple domains in the Crumbs Homolog 2a (Crb2a) protein are required for regulating rod photoreceptor size journal, July 2010

A Polybasic Domain in aPKC Mediates Par-6-Dependent Control of Plasma Membrane Targeting and Kinase Activity posted_content, January 2019

Expression and localization of the polarity protein CRB2 in adult mouse brain: a comparison with the CRB1rd8 mutant mouse model journal, August 2018

Assembly of Epithelial Tight Junctions Is Negatively Regulated by Par6
journal, February 2002

The discs-large tumor suppressor gene of Drosophila encodes a guanylate kinase homolog localized at septate junctions
journal, August 1991

An Atypical PKC Directly Associates and Colocalizes at the Epithelial Tight Junction with ASIP, a Mammalian Homologue of Caenorhabditis elegans Polarity Protein PAR-3
journal, October 1998

Crumbs interacts with moesin and βHeavy-spectrin in the apical membrane skeleton of Drosophila
journal, September 2002

Drosophila PAR-1 and 14-3-3 Inhibit Bazooka/PAR-3 to Establish Complementary Cortical Domains in Polarized Cells
journal, December 2003

Epithelial Cell Polarity and Cell Junctions in Drosophila
journal, December 2001

Direct interaction of two polarity complexes implicated in epithelial tight junction assembly
journal, January 2003

CRB3 Binds Directly to Par6 and Regulates the Morphogenesis of the Tight Junctions in Mammalian Epithelial Cells
journal, March 2004

Interaction of Par-6 and Crumbs complexes is essential for photoreceptor morphogenesis in Drosophila
journal, September 2003

armadillo, bazooka, and stardust are critical for early stages in formation of the zonula adherens and maintenance of the polarized blastoderm epithelium in Drosophila.
journal, July 1996

Discs Lost, a Novel Multi-PDZ Domain Protein, Establishes and Maintains Epithelial Polarity
journal, March 1999

aPKC kinase activity is required for the asymmetric differentiation of the premature junctional complex during epithelial cell polarization
journal, September 2002

Atypical PKC Phosphorylates PAR-1 Kinases to Regulate Localization and Activity
journal, April 2004

Drosophila Atypical Protein Kinase C Associates with Bazooka and Controls Polarity of Epithelia and Neuroblasts
journal, September 2000

Localization of apical epithelial determinants by the basolateral PDZ protein Scribble
journal, February 2000

Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia
journal, December 2003

DmPAR-6 directs epithelial polarity and asymmetric cell division of neuroblasts in Drosophila
journal, December 2000

The Par complex directs asymmetric cell division by phosphorylating the cytoskeletal protein Lgl
journal, March 2003

Apical, Lateral, and Basal Polarization Cues Contribute to the Development of the Follicular Epithelium during Drosophila Oogenesis
journal, November 2000

Composition and Formation of Intercellular Junctions in Epithelial Cells
journal, December 2002

Integrated activity of PDZ protein complexes regulates epithelial polarity
journal, December 2002

Cooperative Regulation of Cell Polarity and Growth by Drosophila Tumor Suppressors
journal, July 2000

Visualization of Gene Expression in Living Adult Drosophila
journal, October 1996

The Crumbs3-Pals1 complex participates in the establishment of polarity in mammalian epithelial cells
journal, July 2003

Molecular cloning of lethal(2)giant larvae, a recessive oncogene of Drosophila melanogaster.
journal, June 1985

Crumbs, a Component of the Apical Membrane, Is Required for Zonula Adherens Formation in Primary Epithelia ofDrosophila
journal, July 1996

Interactions between the crumbs, lethal giant larvae and bazooka pathways in epithelial polarization
journal, December 2002

Drosophila Stardust interacts with Crumbs to control polarity of epithelia but not neuroblasts
journal, December 2001

Origins of Cell Polarity
journal, February 1996

Zonula Adherens Formation in Caenorhabditis elegans Requires dlg-1, the Homologue of the Drosophila Gene discs large
journal, February 2001

Drosophila Stardust is a partner of Crumbs in the control of epithelial cell polarity
journal, December 2001

Composition and function of PDZ protein complexes during cell polarization
journal, September 2003

Loss of PALS1 Expression Leads to Tight Junction and Polarity Defects
journal, April 2004

Control of spindle orientation in Drosophila by the Par-3-related PDZ-domain protein Bazooka
journal, December 1998

A polarity complex of mPar-6 and atypical PKC binds, phosphorylates and regulates mammalian Lgl
journal, March 2003

The Maguk protein, Pals1, functions as an adapter, linking mammalian homologues of Crumbs and Discs Lost
journal, April 2002

The interaction of p62 with RIP links the atypical PKCs to NF-kappa B activation
journal, June 1999

The Drosophila Cell Survival Gene discs lost Encodes a Cytoplasmic Codanin-1-like Protein, Not a Homolog of Tight Junction PDZ Protein Patj
journal, December 2003

Genetic control of epithelial cell polarity: Lessons fromDrosophila
journal, May 2000

Atypical Protein Kinase C Is Involved in the Evolutionarily Conserved Par Protein Complex and Plays a Critical Role in Establishing Epithelia-Specific Junctional Structures
journal, March 2001

Expression of crumbs confers apical character on plasma membrane domains of ectodermal epithelia of drosophila
journal, July 1995

Parsing the Polarity Code
journal, March 2004

Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
journal, June 1993

The Drosophila genes crumbs and stardust are involved in the biogenesis of adherens junctions
journal, March 1996

Involvement of ASIP/PAR-3 in the promotion of epithelial tight junction formation
journal, June 2002

Drosophila neurotactin, a surface glycoprotein with homology to serine esterases, is dynamically expressed during embryogenesis
journal, December 1990

Expression of crumbs confers apical character on plasma membrane domains of ectodermal epithelia of Drosophila
journal, October 1995

Discs Lost, a Novel Multi-PDZ Domain Protein, Establishes and Maintains Epithelial Polarity
journal, December 2003

Molecular cloning of lethal(2)giant larvae, a recessive oncogene of Drosophila melanogaster.
journal, June 1985

Protein kinase C ζ isoform is critical for mitogenic signal transduction
journal, August 1993

Intercellular junctions and cellular polarity: the PAR–aPKC complex, a conserved core cassette playing fundamental roles in cell polarity
journal, October 2001

Positional cloning of heart and soul reveals multiple roles for PKCλ in zebrafish organogenesis
journal, October 2001

A conserved motif in Crumbs is required for E-cadherin localisation and zonula adherens formation in Drosophila
journal, January 2000

Targeted Disruption of the ζPKC Gene Results in the Impairment of the NF-κB Pathway
journal, October 2001

Cell polarity: the PARty expands
journal, January 2001

Drosophila Stardust interacts with Crumbs to control polarity of epithelia but not neuroblasts
journal, December 2001

Drosophila Stardust is a partner of Crumbs in the control of epithelial cell polarity
journal, December 2001

The Par complex directs asymmetric cell division by phosphorylating the cytoskeletal protein Lgl
journal, March 2003

Parsing the Polarity Code
journal, March 2004

Drosophila Atypical Protein Kinase C Associates with Bazooka and Controls Polarity of Epithelia and Neuroblasts
journal, September 2000

Atypical Protein Kinase C Is Involved in the Evolutionarily Conserved Par Protein Complex and Plays a Critical Role in Establishing Epithelia-Specific Junctional Structures
journal, March 2001

Composition and Formation of Intercellular Junctions in Epithelial Cells
journal, December 2002

Epithelial Cell Polarity and Cell Junctions in Drosophila
journal, December 2001

Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
journal, June 1993

The Drosophila genes crumbs and stardust are involved in the biogenesis of adherens junctions
journal, March 1996

The Crumbs3-Pals1 complex participates in the establishment of polarity in mammalian epithelial cells
journal, July 2003

Involvement of ASIP/PAR-3 in the promotion of epithelial tight junction formation
journal, June 2002

The Hippo pathway and apico–basal cell polarity
journal, May 2011

Coordinated protein sorting, targeting and distribution in polarized cells
journal, November 2008

Atypical PKC contributes to poor prognosis through loss of apical-basal polarity and Cyclin E overexpression in ovarian cancer
journal, August 2005

Using Drosophila melanogaster to map human cancer pathways
journal, July 2005

Crumbs3 Is Essential for Proper Epithelial Development and Viability
journal, January 2014

Yorkie and JNK revert syncytial muscles into myoblasts during Org-1–dependent lineage reprogramming
journal, October 2019

Polarization and Myelination in Myelinating Glia
journal, December 2012

Multiple domains in the Crumbs Homolog 2a (Crb2a) protein are required for regulating rod photoreceptor size
journal, July 2010

A Polybasic Domain in aPKC Mediates Par-6-Dependent Control of Plasma Membrane Targeting and Kinase Activity
posted_content, January 2019

Expression and localization of the polarity protein CRB2 in adult mouse brain: a comparison with the CRB1rd8 mutant mouse model
journal, August 2018

Towards understanding CRUMBS function in retinal dystrophies
journal, October 2006

Nuclear fallout provides a new link between aPKC and polarized cell trafficking
journal, April 2016

Epithelial cell polarity: what flies can teach us about cancer
journal, August 2012