Cyanobacterial Alkanes Modulate Photosynthetic Cyclic Electron Flow to Assist Growth under Cold Stress
Abstract
All cyanobacterial membranes contain diesel-range C15-C19 hydrocarbons at concentrations similar to chlorophyll. Recently, two universal but mutually exclusive hydrocarbon production pathways in cyanobacteria were discovered. We engineered a mutant of Synechocystis sp. PCC 6803 that produces no alkanes, which grew poorly at low temperatures. We analyzed this defect by assessing the redox kinetics of PSI. The mutant exhibited enhanced cyclic electron flow (CEF), especially at low temperature. CEF raises the ATP:NADPH ratio from photosynthesis and balances reductant requirements of biosynthesis with maintaining the redox poise of the electron transport chain. We conducted in silico flux balance analysis and showed that growth rate reaches a distinct maximum for an intermediate value of CEF equivalent to recycling 1 electron in 4 from PSI to the plastoquinone pool. Based on this analysis, we conclude that the lack of membrane alkanes causes higher CEF, perhaps for maintenance of redox poise. In turn, increased CEF reduces growth by forcing the cell to use less energy-efficient pathways, lowering the quantum efficiency of photosynthesis. This study highlights the unique and universal role of medium-chain hydrocarbons in cyanobacterial thylakoid membranes: they regulate redox balance and reductant partitioning in these oxygenic photosynthetic cells under stress.
- Authors:
-
- Washington Univ., St. Louis, MO (United States). Dept. of Energy, Environmental, and Chemical Engineering
- Washington Univ., St. Louis, MO (United States). Dept. of Biology; Pennsylvania State Univ., University Park, PA (United States). Dept. of Chemical Engineering
- Pennsylvania State Univ., University Park, PA (United States). Dept. of Chemical Engineering
- Washington Univ., St. Louis, MO (United States). Dept. of Energy, Environmental, and Chemical Engineering; Washington Univ., St. Louis, MO (United States). Dept. of Biology
- Publication Date:
- Research Org.:
- Washington Univ., St. Louis, MO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1624800
- Grant/Contract Number:
- FG02-00ER41132
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 5; Journal Issue: 1; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Science & Technology - Other Topics
Citation Formats
Berla, Bertram M., Saha, Rajib, Maranas, Costas D., and Pakrasi, Himadri B. Cyanobacterial Alkanes Modulate Photosynthetic Cyclic Electron Flow to Assist Growth under Cold Stress. United States: N. p., 2015.
Web. doi:10.1038/srep14894.
Berla, Bertram M., Saha, Rajib, Maranas, Costas D., & Pakrasi, Himadri B. Cyanobacterial Alkanes Modulate Photosynthetic Cyclic Electron Flow to Assist Growth under Cold Stress. United States. https://doi.org/10.1038/srep14894
Berla, Bertram M., Saha, Rajib, Maranas, Costas D., and Pakrasi, Himadri B. Tue .
"Cyanobacterial Alkanes Modulate Photosynthetic Cyclic Electron Flow to Assist Growth under Cold Stress". United States. https://doi.org/10.1038/srep14894. https://www.osti.gov/servlets/purl/1624800.
@article{osti_1624800,
title = {Cyanobacterial Alkanes Modulate Photosynthetic Cyclic Electron Flow to Assist Growth under Cold Stress},
author = {Berla, Bertram M. and Saha, Rajib and Maranas, Costas D. and Pakrasi, Himadri B.},
abstractNote = {All cyanobacterial membranes contain diesel-range C15-C19 hydrocarbons at concentrations similar to chlorophyll. Recently, two universal but mutually exclusive hydrocarbon production pathways in cyanobacteria were discovered. We engineered a mutant of Synechocystis sp. PCC 6803 that produces no alkanes, which grew poorly at low temperatures. We analyzed this defect by assessing the redox kinetics of PSI. The mutant exhibited enhanced cyclic electron flow (CEF), especially at low temperature. CEF raises the ATP:NADPH ratio from photosynthesis and balances reductant requirements of biosynthesis with maintaining the redox poise of the electron transport chain. We conducted in silico flux balance analysis and showed that growth rate reaches a distinct maximum for an intermediate value of CEF equivalent to recycling 1 electron in 4 from PSI to the plastoquinone pool. Based on this analysis, we conclude that the lack of membrane alkanes causes higher CEF, perhaps for maintenance of redox poise. In turn, increased CEF reduces growth by forcing the cell to use less energy-efficient pathways, lowering the quantum efficiency of photosynthesis. This study highlights the unique and universal role of medium-chain hydrocarbons in cyanobacterial thylakoid membranes: they regulate redox balance and reductant partitioning in these oxygenic photosynthetic cells under stress.},
doi = {10.1038/srep14894},
journal = {Scientific Reports},
number = 1,
volume = 5,
place = {United States},
year = {Tue Oct 13 00:00:00 EDT 2015},
month = {Tue Oct 13 00:00:00 EDT 2015}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journalFigures / Tables:
Figure 1: Cartoon of cyanobacterial photosynthetic electron transport pathways. In the linear electron transport pathway (dotted magenta line), light is first absorbed by PSII, then excited electrons are transported inside the membrane by PQ to the cyt b6f complex, then through the thylakoid lumen by the PC to PSI. Atmore »
All figures and tables
(9 total)
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
[34] Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes
book, January 1987
- Lichtenthaler, Hartmut K.
- Methods in Enzymology
Iron stress restricts photosynthetic intersystem electron transport in Synechococcus sp. PCC 7942
journal, November 2000
- Ivanov, A. G.; Park, Y. -I.; Miskiewicz, E.
- FEBS Letters, Vol. 485, Issue 2-3
The histidine kinase Hik33 perceives osmotic stress and cold stress in Synechocystis sp. PCC 6803: Osmostress and cold sensor Hik33 in Synechocystis
journal, November 2002
- Mikami, Koji; Kanesaki, Yu; Suzuki, Iwane
- Molecular Microbiology, Vol. 46, Issue 4
Simple Conditions for Growth of Unicellular Blue-Green Algae on Plates
journal, March 1968
- Allen, Mary Mennes
- Journal of Phycology, Vol. 4, Issue 1
Synergistic effect of high-light and low temperature on cell growth of the Δ12 fatty acid desaturase mutant in Synechococcus sp. PCC 7002
journal, January 2002
- Sakamoto, Toshio; Bryant, Donald A.
- Photosynthesis Research, Vol. 72, Issue 3, p. 231-242
Distinct Roles of Multiple NDH-1 Complexes in the Cyanobacterial Electron Transport Network as Revealed by Kinetic Analysis of P700+ Reduction in Various ndh-Deficient Mutants of Synechocystis sp. Strain PCC6803
journal, October 2010
- Bernat, G.; Appel, J.; Ogawa, T.
- Journal of Bacteriology, Vol. 193, Issue 1
The Redox Potential of the Plastoquinone Pool of the Cyanobacterium Synechocystis Species Strain PCC 6803 Is under Strict Homeostatic Control
journal, April 2014
- Schuurmans, R. Milou; Schuurmans, J. Merijn; Bekker, Martijn
- Plant Physiology, Vol. 165, Issue 1
Reconstruction and Comparison of the Metabolic Potential of Cyanobacteria Cyanothece sp. ATCC 51142 and Synechocystis sp. PCC 6803
journal, October 2012
- Saha, Rajib; Verseput, Alex T.; Berla, Bertram M.
- PLoS ONE, Vol. 7, Issue 10
j5 DNA Assembly Design Automation Software
journal, December 2011
- Hillson, Nathan J.; Rosengarten, Rafael D.; Keasling, Jay D.
- ACS Synthetic Biology, Vol. 1, Issue 1, p. 14-21
The Importance of Energy Balance in Improving Photosynthetic Productivity
journal, November 2010
- Kramer, David M.; Evans, John R.
- Plant Physiology, Vol. 155, Issue 1
Alkane Biosynthesis Genes in Cyanobacteria and Their Transcriptional Organization
journal, July 2014
- Klähn, Stephan; Baumgartner, Desirée; Pfreundt, Ulrike
- Frontiers in Bioengineering and Biotechnology, Vol. 2
Inorganic carbon transporters of the cyanobacterial CO2 concentrating mechanism
journal, February 2011
- Price, G. Dean
- Photosynthesis Research, Vol. 109, Issue 1-3
Engineering cyanobacteria to improve photosynthetic production of alka(e)nes
journal, January 2013
- Wang, Weihua; Liu, Xufeng; Lu, Xuefeng
- Biotechnology for Biofuels, Vol. 6, Issue 1
The effects of alternate optimal solutions in constraint-based genome-scale metabolic models
journal, October 2003
- Mahadevan, R.; Schilling, C. H.
- Metabolic Engineering, Vol. 5, Issue 4
Inhibitors in the functional dissection of the photosynthetic electron transport system
journal, July 2007
- Trebst, Achim
- Photosynthesis Research, Vol. 92, Issue 2
Microbial Biosynthesis of Alkanes
journal, July 2010
- Schirmer, A.; Rude, M. A.; Li, X.
- Science, Vol. 329, Issue 5991, p. 559-562
Cyclic electron flow around photosystem I in unicellular green algae
journal, June 2010
- Alric, Jean
- Photosynthesis Research, Vol. 106, Issue 1-2
Central role of cyclic electron transport around photosystem I in the regulation of photosynthesis
journal, April 2014
- Shikanai, Toshiharu
- Current Opinion in Biotechnology, Vol. 26
Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli
journal, April 2013
- Howard, T. P.; Middelhaufe, S.; Moore, K.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 19
Hydrocarbons of Blue-Green Algae: Geochemical Signfficance
journal, January 1969
- Winters, K.; Parker, P. L.; Van Baalen, C.
- Science, Vol. 163, Issue 3866
Synechococcus sp. Strain PCC 7002 Transcriptome: Acclimation to Temperature, Salinity, Oxidative Stress, and Mixotrophic Growth Conditions
journal, January 2012
- Ludwig, Marcus; Bryant, Donald A.
- Frontiers in Microbiology, Vol. 3
Metabolic Flux Balancing: Basic Concepts, Scientific and Practical Use
journal, October 1994
- Varma, Amit; Palsson, Bernhard O.
- Bio/Technology, Vol. 12, Issue 10
Identification of Novel Ssl0352 Protein (NdhS), Essential for Efficient Operation of Cyclic Electron Transport around Photosystem I, in NADPH:plastoquinone Oxidoreductase (NDH-1) Complexes of Synechocystis sp. PCC 6803
journal, August 2011
- Battchikova, Natalia; Wei, Lanzhen; Du, Lingyu
- Journal of Biological Chemistry, Vol. 286, Issue 42
Two Types of Functionally Distinct NAD(P)H Dehydrogenases in Synechocystis sp. Strain PCC6803
journal, July 2000
- Ohkawa, Hiroshi; Pakrasi, Himadri B.; Ogawa, Teruo
- Journal of Biological Chemistry, Vol. 275, Issue 41
Synthetic biology of cyanobacteria: unique challenges and opportunities
journal, January 2013
- Berla, Bertram M.; Saha, Rajib; Immethun, Cheryl M.
- Frontiers in Microbiology, Vol. 4
Targeted mutagenesis of acyl-lipid desaturases in Synechocystis: evidence for the important roles of polyunsaturated membrane lipids in growth, respiration and photosynthesis.
journal, December 1996
- Tasaka, Y.; Gombos, Z.; Nishiyama, Y.
- The EMBO Journal, Vol. 15, Issue 23
Growth at low temperature causes nitrogen limitation in the cyanobacterium Synechococcus sp. PCC 7002
journal, December 1997
- Sakamoto, Toshio; Bryant, D. A.
- Archives of Microbiology, Vol. 169, Issue 1
Quinol and Cytochrome Oxidases in the Cyanobacterium Synechocystis sp. PCC 6803 †
journal, December 1998
- Howitt, Crispin A.; Vermaas, Wim F. J.
- Biochemistry, Vol. 37, Issue 51
Improved Alkane Production in Nitrogen-Fixing and Halotolerant Cyanobacteria via Abiotic Stresses and Genetic Manipulation of Alkane Synthetic Genes
journal, May 2015
- Kageyama, Hakuto; Waditee-Sirisattha, Rungaroon; Sirisattha, Sophon
- Current Microbiology, Vol. 71, Issue 1
Organic Geochemical Studies, ii. a Preliminary Report on the Distribution of Aliphatic Hydrocarbons in Algae, in Bacteria, and in a Recent lake Sediment
journal, January 1968
- Han, J.; McCarthy, E. D.; Hoeven, W. V.
- Proceedings of the National Academy of Sciences, Vol. 59, Issue 1
Modular Synthase-Encoding Gene Involved in α-Olefin Biosynthesis in Synechococcus sp. Strain PCC 7002
journal, April 2011
- Mendez-Perez, Daniel; Begemann, Matthew B.; Pfleger, Brian F.
- Applied and Environmental Microbiology, Vol. 77, Issue 12, p. 4264-4267
Photosynthesis of ATP—Electrons, Proton Pumps, Rotors, and Poise
journal, August 2002
- Allen, John F.
- Cell, Vol. 110, Issue 3
The recovery of photosynthesis from low-temperature photoinhibition is accelerated by the unsaturation of membrane lipids: a mechanism of chilling tolerance.
journal, September 1994
- Gombos, Z.; Wada, H.; Murata, N.
- Proceedings of the National Academy of Sciences, Vol. 91, Issue 19
Proton-powered turbine of a plant motor
journal, May 2000
- Seelert, Holger; Poetsch, Ansgar; Dencher, Norbert A.
- Nature, Vol. 405, Issue 6785
Detection of Formate, Rather than Carbon Monoxide, As the Stoichiometric Coproduct in Conversion of Fatty Aldehydes to Alkanes by a Cyanobacterial Aldehyde Decarbonylase
journal, March 2011
- Warui, Douglas M.; Li, Ning; Nørgaard, Hanne
- Journal of the American Chemical Society, Vol. 133, Issue 10
Fatty Aldehydes in Cyanobacteria Are a Metabolically Flexible Precursor for a Diversity of Biofuel Products
journal, March 2013
- Kaiser, Brett K.; Carleton, Michael; Hickman, Jason W.
- PLoS ONE, Vol. 8, Issue 3
Metabolic reconstruction of the archaeon methanogen Methanosarcina Acetivorans
journal, January 2011
- Satish Kumar, Vinay; Ferry, James G.; Maranas, Costas D.
- BMC Systems Biology, Vol. 5, Issue 1
Characterization of Cyanobacterial Hydrocarbon Composition and Distribution of Biosynthetic Pathways
journal, January 2014
- Coates, R. Cameron; Podell, Sheila; Korobeynikov, Anton
- PLoS ONE, Vol. 9, Issue 1
Succinate Dehydrogenase and Other Respiratory Pathways in Thylakoid Membranes of Synechocystis sp. Strain PCC 6803: Capacity Comparisons and Physiological Function
journal, July 2001
- Cooley, J. W.; Vermaas, W. F. J.
- Journal of Bacteriology, Vol. 183, Issue 14
Genome-Scale Modeling of Light-Driven Reductant Partitioning and Carbon Fluxes in Diazotrophic Unicellular Cyanobacterium Cyanothece sp. ATCC 51142
journal, April 2012
- Vu, Trang T.; Stolyar, Sergey M.; Pinchuk, Grigoriy E.
- PLoS Computational Biology, Vol. 8, Issue 4
Detailing the optimality of photosynthesis in cyanobacteria through systems biology analysis
journal, January 2012
- Nogales, J.; Gudmundsson, S.; Knight, E. M.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 7
Quantification of cyclic and linear flows in plants
journal, March 2005
- Joliot, P.; Joliot, A.
- Proceedings of the National Academy of Sciences, Vol. 102, Issue 13
Temperature-Induced Changes in the Fatty Acid Composition of the Cyanobacterium, Synechocystis PCC6803
journal, April 1990
- Wada, Hajime; Murata, Norio
- Plant Physiology, Vol. 92, Issue 4
Cyclic electron flow is redox-controlled but independent of state transition
journal, June 2013
- Takahashi, Hiroko; Clowez, Sophie; Wollman, Francis-André
- Nature Communications, Vol. 4, Issue 1
Substrate-Triggered Addition of Dioxygen to the Diferrous Cofactor of Aldehyde-Deformylating Oxygenase to Form a Diferric-Peroxide Intermediate
journal, October 2013
- Pandelia, Maria E.; Li, Ning; Nørgaard, Hanne
- Journal of the American Chemical Society, Vol. 135, Issue 42
What is flux balance analysis?
journal, March 2010
- Orth, Jeffrey D.; Thiele, Ines; Palsson, Bernhard Ø
- Nature Biotechnology, Vol. 28, Issue 3
Sequence Analysis of the Genome of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC6803. II. Sequence Determination of the Entire Genome and Assignment of Potential Protein-coding Regions
journal, January 1996
- Kaneko, T.; Sato, S.; Kotani, H.
- DNA Research, Vol. 3, Issue 3
SLIC: A Method for Sequence- and Ligation-Independent Cloning
book, January 2012
- Li, Mamie Z.; Elledge, Stephen J.
- Methods in Molecular Biology
Unique Thylakoid Membrane Architecture of a Unicellular N 2 -Fixing Cyanobacterium Revealed by Electron Tomography
journal, December 2010
- Liberton, Michelle; Austin, Jotham R.; Berg, R. Howard
- Plant Physiology, Vol. 155, Issue 4
Role of cyclic electron transport in photosynthesis as measured by the photoinduced turnover of P700 in vivo
journal, September 1976
- Maxwell, Peter C.; Biggins, John
- Biochemistry, Vol. 15, Issue 18
Insights into the physiology of Chlorella vulgaris cultivated in sweet sorghum bagasse hydrolysate for sustainable algal biomass and lipid production
journal, March 2021
- Arora, Neha; Philippidis, George P.
- Scientific Reports, Vol. 11, Issue 1
Detection of formate, rather than carbon monoxide, as the stoichiometric coproduct in conversion of fatty aldehydes to alkanes by a cyanobacterial aldehyde decarbonylase.
journal, March 2011
- Warui, D. M.
- Journal of the American Chemical Society
Targeted mutagenesis of acyl-lipid desaturases in Synechocystis: evidence for the important roles of polyunsaturated membrane lipids in growth, respiration and photosynthesis.
journal, December 1996
- Tasaka, Y.; Gombos, Z.; Nishiyama, Y.
- The EMBO Journal, Vol. 15, Issue 23
Growth at low temperature causes nitrogen limitation in the cyanobacterium Synechococcus sp. PCC 7002
journal, December 1997
- Sakamoto, Toshio; Bryant, D. A.
- Archives of Microbiology, Vol. 169, Issue 1
Cyclic electron flow around photosystem I in unicellular green algae
journal, June 2010
- Alric, Jean
- Photosynthesis Research, Vol. 106, Issue 1-2
Inorganic carbon transporters of the cyanobacterial CO2 concentrating mechanism
journal, February 2011
- Price, G. Dean
- Photosynthesis Research, Vol. 109, Issue 1-3
Central role of cyclic electron transport around photosystem I in the regulation of photosynthesis
journal, April 2014
- Shikanai, Toshiharu
- Current Opinion in Biotechnology, Vol. 26
The effects of alternate optimal solutions in constraint-based genome-scale metabolic models
journal, October 2003
- Mahadevan, R.; Schilling, C. H.
- Metabolic Engineering, Vol. 5, Issue 4
Role of cyclic electron transport in photosynthesis as measured by the photoinduced turnover of P700 in vivo
journal, September 1976
- Maxwell, Peter C.; Biggins, John
- Biochemistry, Vol. 15, Issue 18
Quinol and Cytochrome Oxidases in the Cyanobacterium Synechocystis sp. PCC 6803 †
journal, December 1998
- Howitt, Crispin A.; Vermaas, Wim F. J.
- Biochemistry, Vol. 37, Issue 51
Detection of Formate, Rather than Carbon Monoxide, As the Stoichiometric Coproduct in Conversion of Fatty Aldehydes to Alkanes by a Cyanobacterial Aldehyde Decarbonylase
journal, March 2011
- Warui, Douglas M.; Li, Ning; Nørgaard, Hanne
- Journal of the American Chemical Society, Vol. 133, Issue 10
Substrate-Triggered Addition of Dioxygen to the Diferrous Cofactor of Aldehyde-Deformylating Oxygenase to Form a Diferric-Peroxide Intermediate
journal, October 2013
- Pandelia, Maria E.; Li, Ning; Nørgaard, Hanne
- Journal of the American Chemical Society, Vol. 135, Issue 42
j5 DNA Assembly Design Automation Software
journal, December 2011
- Hillson, Nathan J.; Rosengarten, Rafael D.; Keasling, Jay D.
- ACS Synthetic Biology, Vol. 1, Issue 1, p. 14-21
Metabolic Flux Balancing: Basic Concepts, Scientific and Practical Use
journal, October 1994
- Varma, Amit; Palsson, Bernhard O.
- Bio/Technology, Vol. 12, Issue 10
Cyclic electron flow is redox-controlled but independent of state transition
journal, June 2013
- Takahashi, Hiroko; Clowez, Sophie; Wollman, Francis-André
- Nature Communications, Vol. 4, Issue 1
Quantification of cyclic and linear flows in plants
journal, March 2005
- Joliot, P.; Joliot, A.
- Proceedings of the National Academy of Sciences, Vol. 102, Issue 13
Detailing the optimality of photosynthesis in cyanobacteria through systems biology analysis
journal, January 2012
- Nogales, J.; Gudmundsson, S.; Knight, E. M.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 7
Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli
journal, April 2013
- Howard, T. P.; Middelhaufe, S.; Moore, K.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 19
Sequence Analysis of the Genome of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC6803. II. Sequence Determination of the Entire Genome and Assignment of Potential Protein-coding Regions
journal, January 1996
- Kaneko, T.; Sato, S.; Kotani, H.
- DNA Research, Vol. 3, Issue 3
Unique Thylakoid Membrane Architecture of a Unicellular N 2 -Fixing Cyanobacterium Revealed by Electron Tomography
journal, December 2010
- Liberton, Michelle; Austin, Jotham R.; Berg, R. Howard
- Plant Physiology, Vol. 155, Issue 4
The Importance of Energy Balance in Improving Photosynthetic Productivity
journal, November 2010
- Kramer, David M.; Evans, John R.
- Plant Physiology, Vol. 155, Issue 1
The Redox Potential of the Plastoquinone Pool of the Cyanobacterium Synechocystis Species Strain PCC 6803 Is under Strict Homeostatic Control
journal, April 2014
- Schuurmans, R. Milou; Schuurmans, J. Merijn; Bekker, Martijn
- Plant Physiology, Vol. 165, Issue 1
Metabolic reconstruction of the archaeon methanogen Methanosarcina Acetivorans
journal, January 2011
- Satish Kumar, Vinay; Ferry, James G.; Maranas, Costas D.
- BMC Systems Biology, Vol. 5, Issue 1
Engineering cyanobacteria to improve photosynthetic production of alka(e)nes
journal, January 2013
- Wang, Weihua; Liu, Xufeng; Lu, Xuefeng
- Biotechnology for Biofuels, Vol. 6, Issue 1
Genome-Scale Modeling of Light-Driven Reductant Partitioning and Carbon Fluxes in Diazotrophic Unicellular Cyanobacterium Cyanothece sp. ATCC 51142
journal, April 2012
- Vu, Trang T.; Stolyar, Sergey M.; Pinchuk, Grigoriy E.
- PLoS Computational Biology, Vol. 8, Issue 4
Fatty Aldehydes in Cyanobacteria Are a Metabolically Flexible Precursor for a Diversity of Biofuel Products
journal, March 2013
- Kaiser, Brett K.; Carleton, Michael; Hickman, Jason W.
- PLoS ONE, Vol. 8, Issue 3
Characterization of Cyanobacterial Hydrocarbon Composition and Distribution of Biosynthetic Pathways
journal, January 2014
- Coates, R. Cameron; Podell, Sheila; Korobeynikov, Anton
- PLoS ONE, Vol. 9, Issue 1
Foliar application of zinc in the agronomic biofortification of arugula
journal, December 2019
- Rugeles-Reyes, Sergio Manuel; CecÍLio Filho, Arthur Bernardes; LÓPez Aguilar, Miguel Angel
- Food Science and Technology, Vol. 39, Issue 4
Alkane Biosynthesis Genes in Cyanobacteria and Their Transcriptional Organization
journal, July 2014
- Klähn, Stephan; Baumgartner, Desirée; Pfreundt, Ulrike
- Frontiers in Bioengineering and Biotechnology, Vol. 2
Synthetic biology of cyanobacteria: unique challenges and opportunities
journal, January 2013
- Berla, Bertram M.; Saha, Rajib; Immethun, Cheryl M.
- Frontiers in Microbiology, Vol. 4
Works referencing / citing this record:
Diverse hydrocarbon biosynthetic enzymes can substitute for olefin synthase in the cyanobacterium Synechococcus sp. PCC 7002
journal, February 2019
- Knoot, Cory J.; Pakrasi, Himadri B.
- Scientific Reports, Vol. 9, Issue 1
Modeling the Interplay between Photosynthesis, CO2 Fixation, and the Quinone Pool in a Purple Non-Sulfur Bacterium
journal, September 2019
- Alsiyabi, Adil; Immethun, Cheryl M.; Saha, Rajib
- Scientific Reports, Vol. 9, Issue 1
Requirement of alkanes for salt tolerance of Cyanobacteria: characterization of alkane synthesis genes from salt-sensitive Synechococcus elongatus PCC7942 and salt-tolerant Aphanothece halophytica
journal, July 2018
- Yamamori, T.; Kageyama, H.; Tanaka, Y.
- Letters in Applied Microbiology, Vol. 67, Issue 3
Improved lipid production via fatty acid biosynthesis and free fatty acid recycling in engineered Synechocystis sp. PCC 6803
journal, January 2019
- Eungrasamee, Kamonchanock; Miao, Rui; Incharoensakdi, Aran
- Biotechnology for Biofuels, Vol. 12, Issue 1
Diverse hydrocarbon biosynthetic enzymes can substitute for olefin synthase in the cyanobacterium Synechococcus sp. PCC 7002
journal, February 2019
- Knoot, Cory J.; Pakrasi, Himadri B.
- Scientific Reports, Vol. 9, Issue 1
Microalgae synthesize hydrocarbons from long-chain fatty acids via a light-dependent pathway
journal, June 2016
- Sorigué, Damien; Légeret, Bertrand; Cuiné, Stéphan
- Plant Physiology
Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.