Molecular tools enabling pennycress (Thlaspi arvense) as a model plant and oilseed cash cover crop
Abstract
Thlapsi arvense L. (pennycress) is being developed as a profitable oilseed cover crop for the winter fallow period throughout the temperate regions of the world, controlling soil erosion and nutrients run-off on otherwise barren farmland. We demonstrate that pennycress can serve as a user-friendly model system akin to Arabidopsis that is well-suited for both laboratory and field experimentation. We sequenced the diploid genome of the spring-type Spring 32-10 inbred line (1C DNA content of 539 Mb; 2n = 14), identifying variation that may explain phenotypic differences with winter-type pennycress, as well as predominantly a one-to-one correspondence with Arabidopsis genes, which makes translational research straightforward. We developed an Agrobacterium-mediated floral dip transformation method (0.5% transformation efficiency) and introduced CRISPR-Cas9 constructs to produce indel mutations in the putative FATTY ACID ELONGATION1 (FAE1) gene, thereby abolishing erucic acid production and creating an edible seed oil comparable to that of canola. We also stably transformed pennycress with the Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene, producing low-viscosity acetyl-triacylglycerol-containing seed oil suitable as a diesel-engine drop-in fuel. Adoption of pennycress as a model system will accelerate oilseed-crop translational research and facilitate pennycress’ rapid domestication to meet the growing sustainable food and fuel demands.
- Authors:
-
[5];
[1]
- Illinois State University (ISU), Normal, IL (United States)
- Western Illinois University, Macomb, IL (United States)
- Univ. of Minnesota, Saint Paul, MN (United States)
- Kansas State Univ., Manhattan, KS (United States)
- Univ. of Nebraska, Lincoln, NE (United States)
- Publication Date:
- Research Org.:
- Colorado State Univ., Fort Collins, CO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); USDA
- OSTI Identifier:
- 1611817
- Grant/Contract Number:
- SC0012459; 2014-67009-22305; 2015-67013-22815
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Plant Biotechnology Journal
- Additional Journal Information:
- Journal Volume: 17; Journal Issue: 4; Journal ID: ISSN 1467-7644
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biotechnology and applied microbiology; plant sciences; pennycress; genome; domestication; triacylglycerol; CRISPR; transformation
Citation Formats
McGinn, Michaela, Phippen, Winthrop B., Chopra, Ratan, Bansal, Sunil, Jarvis, Brice A., Phippen, Mary E., Dorn, Kevin M., Esfahanian, Maliheh, Nazarenus, Tara J., Cahoon, Edgar B., Durrett, Timothy P., Marks, M. David, and Sedbrook, John C. Molecular tools enabling pennycress (Thlaspi arvense) as a model plant and oilseed cash cover crop. United States: N. p., 2018.
Web. doi:10.1111/pbi.13014.
McGinn, Michaela, Phippen, Winthrop B., Chopra, Ratan, Bansal, Sunil, Jarvis, Brice A., Phippen, Mary E., Dorn, Kevin M., Esfahanian, Maliheh, Nazarenus, Tara J., Cahoon, Edgar B., Durrett, Timothy P., Marks, M. David, & Sedbrook, John C. Molecular tools enabling pennycress (Thlaspi arvense) as a model plant and oilseed cash cover crop. United States. https://doi.org/10.1111/pbi.13014
McGinn, Michaela, Phippen, Winthrop B., Chopra, Ratan, Bansal, Sunil, Jarvis, Brice A., Phippen, Mary E., Dorn, Kevin M., Esfahanian, Maliheh, Nazarenus, Tara J., Cahoon, Edgar B., Durrett, Timothy P., Marks, M. David, and Sedbrook, John C. Wed .
"Molecular tools enabling pennycress (Thlaspi arvense) as a model plant and oilseed cash cover crop". United States. https://doi.org/10.1111/pbi.13014. https://www.osti.gov/servlets/purl/1611817.
@article{osti_1611817,
title = {Molecular tools enabling pennycress (Thlaspi arvense) as a model plant and oilseed cash cover crop},
author = {McGinn, Michaela and Phippen, Winthrop B. and Chopra, Ratan and Bansal, Sunil and Jarvis, Brice A. and Phippen, Mary E. and Dorn, Kevin M. and Esfahanian, Maliheh and Nazarenus, Tara J. and Cahoon, Edgar B. and Durrett, Timothy P. and Marks, M. David and Sedbrook, John C.},
abstractNote = {Thlapsi arvense L. (pennycress) is being developed as a profitable oilseed cover crop for the winter fallow period throughout the temperate regions of the world, controlling soil erosion and nutrients run-off on otherwise barren farmland. We demonstrate that pennycress can serve as a user-friendly model system akin to Arabidopsis that is well-suited for both laboratory and field experimentation. We sequenced the diploid genome of the spring-type Spring 32-10 inbred line (1C DNA content of 539 Mb; 2n = 14), identifying variation that may explain phenotypic differences with winter-type pennycress, as well as predominantly a one-to-one correspondence with Arabidopsis genes, which makes translational research straightforward. We developed an Agrobacterium-mediated floral dip transformation method (0.5% transformation efficiency) and introduced CRISPR-Cas9 constructs to produce indel mutations in the putative FATTY ACID ELONGATION1 (FAE1) gene, thereby abolishing erucic acid production and creating an edible seed oil comparable to that of canola. We also stably transformed pennycress with the Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene, producing low-viscosity acetyl-triacylglycerol-containing seed oil suitable as a diesel-engine drop-in fuel. Adoption of pennycress as a model system will accelerate oilseed-crop translational research and facilitate pennycress’ rapid domestication to meet the growing sustainable food and fuel demands.},
doi = {10.1111/pbi.13014},
journal = {Plant Biotechnology Journal},
number = 4,
volume = 17,
place = {United States},
year = {Wed Sep 19 00:00:00 EDT 2018},
month = {Wed Sep 19 00:00:00 EDT 2018}
}
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Metabolic engineering of oilseed crops to produce high levels of novel acetyl glyceride oils with reduced viscosity, freezing point and calorific value
journal, March 2015
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De novo assembly of the pennycress ( Thlaspi arvense ) transcriptome provides tools for the development of a winter cover crop and biodiesel feedstock
journal, August 2013
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Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana
journal, June 2014
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Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system
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The pennycress (Thlaspi arvense L.) nectary: structural and transcriptomic characterization
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