Automated analysis of scanning electron microscopic images for assessment of hair surface damage
Abstract
Mechanical damage of hair can serve as an indicator of health status and its assessment relies on the measurement of morphological features via microscopic analysis, yet few studies have categorized the extent of damage sustained, and instead have depended on qualitative profiling based on the presence or absence of specific features. We describe the development and application of a novel quantitative measure for scoring hair surface damage in scanning electron microscopic (SEM) images without predefined features, and automation of image analysis for characterization of morphological hair damage after exposure to an explosive blast. Application of an automated normalization procedure for SEM images revealed features indicative of contact with materials in an explosive device and characteristic of heat damage, though many were similar to features from physical and chemical weathering. Assessment of hair damage with tailing factor, a measure of asymmetry in pixel brightness histograms and proxy for surface roughness, yielded 81% classification accuracy to an existing damage classification system, indicating good agreement between the two metrics. Further ability of the tailing factor to score features of hair damage reflecting explosion conditions demonstrates the broad applicability of the metric to assess damage to hairs containing a diverse set of morphological features.
- Authors:
-
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Michigan State Univ., East Lansing, MI (United States)
- Lawrence Livermore National Laboratory, 7000 East Ave., Livermore, CA 94550, USA; Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Michigan State Univ., East Lansing, MI (United States)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA); USDOE Laboratory Directed Research and Development (LDRD) Program
- OSTI Identifier:
- 1599561
- Alternate Identifier(s):
- OSTI ID: 1557395
- Report Number(s):
- LLNL-JRNL-787599; LLNL-JRNL-774282
Journal ID: ISSN 2054-5703; 984952
- Grant/Contract Number:
- AC52-07NA27344; 16-SI-002
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Royal Society Open Science
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2054-5703
- Publisher:
- The Royal Society Publishing
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 97 MATHEMATICS AND COMPUTING; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; scanning electron microscopy; hair damage; image analysis; microscopic hair analysis; surface roughness; Biological and medical sciences, Mathematics and Computing, Chemistry - Inorganic, organic, physical and analytical chemistry
Citation Formats
Chu, Fanny, Anex, Deon S., Jones, A. Daniel, and Hart, Bradley R. Automated analysis of scanning electron microscopic images for assessment of hair surface damage. United States: N. p., 2020.
Web. doi:10.1098/rsos.191438.
Chu, Fanny, Anex, Deon S., Jones, A. Daniel, & Hart, Bradley R. Automated analysis of scanning electron microscopic images for assessment of hair surface damage. United States. https://doi.org/10.1098/rsos.191438
Chu, Fanny, Anex, Deon S., Jones, A. Daniel, and Hart, Bradley R. Wed .
"Automated analysis of scanning electron microscopic images for assessment of hair surface damage". United States. https://doi.org/10.1098/rsos.191438. https://www.osti.gov/servlets/purl/1599561.
@article{osti_1599561,
title = {Automated analysis of scanning electron microscopic images for assessment of hair surface damage},
author = {Chu, Fanny and Anex, Deon S. and Jones, A. Daniel and Hart, Bradley R.},
abstractNote = {Mechanical damage of hair can serve as an indicator of health status and its assessment relies on the measurement of morphological features via microscopic analysis, yet few studies have categorized the extent of damage sustained, and instead have depended on qualitative profiling based on the presence or absence of specific features. We describe the development and application of a novel quantitative measure for scoring hair surface damage in scanning electron microscopic (SEM) images without predefined features, and automation of image analysis for characterization of morphological hair damage after exposure to an explosive blast. Application of an automated normalization procedure for SEM images revealed features indicative of contact with materials in an explosive device and characteristic of heat damage, though many were similar to features from physical and chemical weathering. Assessment of hair damage with tailing factor, a measure of asymmetry in pixel brightness histograms and proxy for surface roughness, yielded 81% classification accuracy to an existing damage classification system, indicating good agreement between the two metrics. Further ability of the tailing factor to score features of hair damage reflecting explosion conditions demonstrates the broad applicability of the metric to assess damage to hairs containing a diverse set of morphological features.},
doi = {10.1098/rsos.191438},
journal = {Royal Society Open Science},
number = 1,
volume = 7,
place = {United States},
year = {Wed Jan 15 00:00:00 EST 2020},
month = {Wed Jan 15 00:00:00 EST 2020}
}
Web of Science
Works referenced in this record:
Hair Shaft Damage from Heat and Drying Time of Hair Dryer
journal, January 2011
- Lee, Yoonhee; Kim, Youn-Duk; Hyun, Hye-Jin
- Annals of Dermatology, Vol. 23, Issue 4
Quantitative analysis and classification of AFM images of human hair
journal, July 2004
- Gurden, S. P.; Monteiro, V. F.; Longo, E.
- Journal of Microscopy, Vol. 215, Issue 1
The color(s) of human hair—Forensic hair analysis with SpectraCube®
journal, March 2009
- Birngruber, Christoph; Ramsthaler, Frank; Verhoff, Marcel A.
- Forensic Science International, Vol. 185, Issue 1-3
Effect of shampoo, conditioner and permanent waving on the molecular structure of human hair
journal, January 2015
- Zhang, Yuchen; Alsop, Richard J.; Soomro, Asfia
- PeerJ, Vol. 3
Assessment of hair surface roughness using quantitative image analysis
journal, July 2017
- Park, K. H.; Kim, H. J.; Oh, B.
- Skin Research and Technology, Vol. 24, Issue 1
Development of a Classification System for Extrinsic Hair Damage: Standard Grading of Electron Microscopic Findings of Damaged Hairs
journal, January 2010
- Kim, Youn-Duk; Jeon, Soo-Young; Ji, Jae Hong
- The American Journal of Dermatopathology, Vol. 32, Issue 5
Score normalization in multimodal biometric systems
journal, December 2005
- Jain, Anil; Nandakumar, Karthik; Ross, Arun
- Pattern Recognition, Vol. 38, Issue 12
Effects of solar radiation on hair and photoprotection
journal, December 2015
- Dario, Michelli F.; Baby, André R.; Velasco, Maria Valéria R.
- Journal of Photochemistry and Photobiology B: Biology, Vol. 153
Twelve-point scale grading system of scanning electron microscopic examination to investigate subtle changes in damaged hair surface
journal, January 2016
- Lee, S. Y.; Choi, A. R.; Baek, J. H.
- Skin Research and Technology, Vol. 22, Issue 4
Differentiation of human hair by colour and diameter using light microscopy, digital imaging and statistical analysis: DIFFERENTIATION OF HUMAN HAIR
journal, September 2017
- Mills, M.; Bonetti, J.; Brettell, T.
- Journal of Microscopy, Vol. 270, Issue 1
Luminosity and contrast normalization in retinal images
journal, June 2005
- Foracchia, Marco; Grisan, Enrico; Ruggeri, Alfredo
- Medical Image Analysis, Vol. 9, Issue 3
Hair-MAP: a prototype automated system for forensic hair comparison and analysis
journal, October 2002
- Verma, Mool S.; Pratt, Lorien; Ganesh, Chidamber
- Forensic Science International, Vol. 129, Issue 3
Effect of photodamage on the outermost cuticle layer of human hair
journal, December 2015
- Richena, M.; Rezende, C. A.
- Journal of Photochemistry and Photobiology B: Biology, Vol. 153
The identification of mammalian species through the classification of hair patterns using image pattern recognition
conference, January 2006
- Moyo, Thamsanqa; Bangay, Shaun; Foster, Greg
- Proceedings of the 4th international conference on Computer graphics, virtual reality, visualisation and interaction in Africa - Afrigaph '06
Digital imaging and image analysis applied to numerical applications in forensic hair examination
journal, March 2011
- Brooks, Elizabeth; Comber, Bruce; McNaught, Ian
- Science & Justice, Vol. 51, Issue 1
Cuticle cell defects in lamellar ichthyosis hair and anomalous hair shaft syndromes visualized after detergent extraction
journal, December 1996
- Rice, Robert H.; Wong, Viviana J.; Price, Vera H.
- The Anatomical Record, Vol. 246, Issue 4
Influence of Oxidative and/or Reductive Treatment on Human Hair (I): Analysis of Hair-Damage after Oxidative and/or Reductive Treatment
journal, January 2003
- Takada, Koji; Nakamura, Akira; Matsuo, Naoko
- Journal of Oleo Science, Vol. 52, Issue 10
Secondary electron emission in the scanning electron microscope
journal, November 1983
- Seiler, H.
- Journal of Applied Physics, Vol. 54, Issue 11
In situ labeling of DNA reveals interindividual variation in nuclear DNA breakdown in hair and may be useful to predict success of forensic genotyping of hair
journal, April 2011
- Szabo, Sandra; Jaeger, Karin; Fischer, Heinz
- International Journal of Legal Medicine, Vol. 126, Issue 1
The ImageJ ecosystem: An open platform for biomedical image analysis: T
journal, July 2015
- Schindelin, Johannes; Rueden, Curtis T.; Hiner, Mark C.
- Molecular Reproduction and Development, Vol. 82, Issue 7-8
Scanning Electron Microscopy for Quantitative Small and Large Deformation Measurements Part I: SEM Imaging at Magnifications from 200 to 10,000
journal, March 2007
- Sutton, M. A.; Li, N.; Joy, D. C.
- Experimental Mechanics, Vol. 47, Issue 6
Statistical normalization techniques for magnetic resonance imaging
journal, January 2014
- Shinohara, Russell T.; Sweeney, Elizabeth M.; Goldsmith, Jeff
- NeuroImage: Clinical, Vol. 6
Scanning electron microscopy study of hair shaft damage secondary to cosmetic treatments of the hair
journal, January 2016
- Kaliyadan, Feroze; Gosai, Bb; Al Melhim, WalidNaief
- International Journal of Trichology, Vol. 8, Issue 2
NIH Image to ImageJ: 25 years of image analysis
journal, June 2012
- Schneider, Caroline A.; Rasband, Wayne S.; Eliceiri, Kevin W.
- Nature Methods, Vol. 9, Issue 7
A quantitative assessment of a reliable screening technique for the STR analysis of telogen hair roots
journal, January 2013
- Edson, Janette; Brooks, Elizabeth M.; McLaren, Carolyn
- Forensic Science International: Genetics, Vol. 7, Issue 1
Automated analysis of scanning electron microscopic images for assessment of hair surface damage
dataset, January 2019
- Chu, Fanny; Anex, Deon; Jones, A. Daniel
- Dryad
Scanning electron microscopic studies of wool/intumescent char formation
journal, January 2000
- Davies, Philip J.; Horrocks, A. Richard; Miraftab, Mohsen
- Polymer International, Vol. 49, Issue 10
In situ labeling of DNA reveals interindividual variation in nuclear DNA breakdown in hair and may be useful to predict success of forensic genotyping of hair
journal, April 2011
- Szabo, Sandra; Jaeger, Karin; Fischer, Heinz
- International Journal of Legal Medicine, Vol. 126, Issue 1
Drilling accurate nanopores for biosensors by energetic multi-wall carbon nanotubes: a molecular dynamics investigation
journal, September 2022
- Li, Changsheng; Wang, Zilin; Ma, Lei
- Journal of Molecular Modeling, Vol. 28, Issue 10
Scanning Electron Microscopy for Quantitative Small and Large Deformation Measurements Part I: SEM Imaging at Magnifications from 200 to 10,000
journal, March 2007
- Sutton, M. A.; Li, N.; Joy, D. C.
- Experimental Mechanics, Vol. 47, Issue 6
A quantitative assessment of a reliable screening technique for the STR analysis of telogen hair roots
journal, January 2013
- Edson, Janette; Brooks, Elizabeth M.; McLaren, Carolyn
- Forensic Science International: Genetics, Vol. 7, Issue 1
Effects of solar radiation on hair and photoprotection
journal, December 2015
- Dario, Michelli F.; Baby, André R.; Velasco, Maria Valéria R.
- Journal of Photochemistry and Photobiology B: Biology, Vol. 153
Effect of photodamage on the outermost cuticle layer of human hair
journal, December 2015
- Richena, M.; Rezende, C. A.
- Journal of Photochemistry and Photobiology B: Biology, Vol. 153
Statistical normalization techniques for magnetic resonance imaging
journal, January 2014
- Shinohara, Russell T.; Sweeney, Elizabeth M.; Goldsmith, Jeff
- NeuroImage: Clinical, Vol. 6
Modified resolution factor for asymmetrical peaks in chromatographic separation
journal, August 2003
- Song, Di; Wang, Juan
- Journal of Pharmaceutical and Biomedical Analysis, Vol. 32, Issue 6
Three types of genes underlying the Gametophyte factor1 locus cause unilateral cross incompatibility in maize
journal, August 2022
- Wang, Yuebin; Li, Wenqiang; Wang, Luxi
- Nature Communications, Vol. 13, Issue 1
Secondary electron emission in the scanning electron microscope
journal, November 1983
- Seiler, H.
- Journal of Applied Physics, Vol. 54, Issue 11
Quantitative analysis and classification of AFM images of human hair
journal, July 2004
- Gurden, S. P.; Monteiro, V. F.; Longo, E.
- Journal of Microscopy, Vol. 215, Issue 1
Differentiation of human hair by colour and diameter using light microscopy, digital imaging and statistical analysis: DIFFERENTIATION OF HUMAN HAIR
journal, September 2017
- Mills, M.; Bonetti, J.; Brettell, T.
- Journal of Microscopy, Vol. 270, Issue 1
Assessment of hair surface roughness using quantitative image analysis
journal, July 2017
- Park, K. H.; Kim, H. J.; Oh, B.
- Skin Research and Technology, Vol. 24, Issue 1
Pattern Recognition Techniques for the Identification of Activities of Daily Living using Mobile Device Accelerometer
preprint, January 2017
- Pires, Ivan Miguel; Garcia, Nuno M.; Pombo, Nuno
- arXiv
Hair Shaft Damage from Heat and Drying Time of Hair Dryer
journal, January 2011
- Lee, Yoonhee; Kim, Youn-Duk; Hyun, Hye-Jin
- Annals of Dermatology, Vol. 23, Issue 4
Influence of Oxidative and/or Reductive Treatment on Human Hair (I): Analysis of Hair-Damage after Oxidative and/or Reductive Treatment
journal, January 2003
- Takada, Koji; Nakamura, Akira; Matsuo, Naoko
- Journal of Oleo Science, Vol. 52, Issue 10