Soft matter and nanomaterials characterization by cryogenic transmission electron microscopy
Abstract
Soft matter has historically been an unlikely candidate for investigation by electron microscopy techniques due to damage by the electron beam as well as inherent instability under a high vacuum environment. Characterization of soft matter has often relied on ensemble-scattering techniques. The recent development of cryogenic transmission electron microscopy (cryo-TEM) provides the soft matter community with an exciting opportunity to probe the structure of soft materials in real space. Cryo-TEM reduces beam damage and allows for characterization in a native, frozen-hydrated state, providing direct visual representation of soft structure. This article reviews cryo-TEM in soft materials characterization and illustrates how it has provided unique insights not possible by traditional ensemble techniques. Soft matter systems that have benefited from the use of cryo-TEM include biological-based “soft” nanoparticles (e.g., viruses and conjugates), synthetic polymers, supramolecular materials as well as the organic–inorganic interface of colloidal nanoparticles. We conclude that while many challenges remain, such as combining structural and chemical analyses; the opportunity for soft matter research to leverage newly developed cryo-TEM techniques continues to excite.
- Authors:
-
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Center for Integrated Nanotechnologies
- Case Western Reserve Univ., Cleveland, OH (United States)
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1581981
- Report Number(s):
- SAND-2019-13745J
Journal ID: ISSN 0883-7694; 681344; TRN: US2100921
- Grant/Contract Number:
- AC04-94AL85000; NA0003525; 89233218CNA000001
- Resource Type:
- Accepted Manuscript
- Journal Name:
- MRS Bulletin
- Additional Journal Information:
- Journal Volume: 44; Journal Issue: 12; Journal ID: ISSN 0883-7694
- Publisher:
- Materials Research Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Watt, John, Huber, Dale L., and Stewart, Phoebe L. Soft matter and nanomaterials characterization by cryogenic transmission electron microscopy. United States: N. p., 2019.
Web. doi:10.1557/mrs.2019.285.
Watt, John, Huber, Dale L., & Stewart, Phoebe L. Soft matter and nanomaterials characterization by cryogenic transmission electron microscopy. United States. https://doi.org/10.1557/mrs.2019.285
Watt, John, Huber, Dale L., and Stewart, Phoebe L. Tue .
"Soft matter and nanomaterials characterization by cryogenic transmission electron microscopy". United States. https://doi.org/10.1557/mrs.2019.285. https://www.osti.gov/servlets/purl/1581981.
@article{osti_1581981,
title = {Soft matter and nanomaterials characterization by cryogenic transmission electron microscopy},
author = {Watt, John and Huber, Dale L. and Stewart, Phoebe L.},
abstractNote = {Soft matter has historically been an unlikely candidate for investigation by electron microscopy techniques due to damage by the electron beam as well as inherent instability under a high vacuum environment. Characterization of soft matter has often relied on ensemble-scattering techniques. The recent development of cryogenic transmission electron microscopy (cryo-TEM) provides the soft matter community with an exciting opportunity to probe the structure of soft materials in real space. Cryo-TEM reduces beam damage and allows for characterization in a native, frozen-hydrated state, providing direct visual representation of soft structure. This article reviews cryo-TEM in soft materials characterization and illustrates how it has provided unique insights not possible by traditional ensemble techniques. Soft matter systems that have benefited from the use of cryo-TEM include biological-based “soft” nanoparticles (e.g., viruses and conjugates), synthetic polymers, supramolecular materials as well as the organic–inorganic interface of colloidal nanoparticles. We conclude that while many challenges remain, such as combining structural and chemical analyses; the opportunity for soft matter research to leverage newly developed cryo-TEM techniques continues to excite.},
doi = {10.1557/mrs.2019.285},
journal = {MRS Bulletin},
number = 12,
volume = 44,
place = {United States},
year = {Tue Dec 10 00:00:00 EST 2019},
month = {Tue Dec 10 00:00:00 EST 2019}
}
Web of Science
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