Monomeric YoeB toxin retains RNase activity but adopts an obligate dimeric form for thermal stability
Abstract
Chromosomally-encoded toxin-antitoxin complexes are ubiquitous in bacteria and regulate growth through the release of the toxin component typically in a stress-dependent manner. Type II ribosome-dependent toxins adopt a RelE-family RNase fold and inhibit translation by degrading mRNAs while bound to the ribosome. Here, we present biochemical and structural studies of the Escherichia coli YoeB toxin interacting with both a UAA stop and an AAU sense codon in pre- and post-mRNA cleavage states to provide insights into possible mRNA substrate selection. Both mRNAs undergo minimal changes during the cleavage event in contrast to type II ribosome-dependent RelE toxin. Further, the 16S rRNA decoding site nucleotides that monitor the mRNA in the aminoacyl(A) site adopt different orientations depending upon which toxin is present. Although YoeB is a RelE family member, it is the sole ribosome-dependent toxin that is dimeric. We show that engineered monomeric YoeB is active against mRNAs bound to both the small and large subunit. However, the stability of monomeric YoeB is reduced ~20°C, consistent with potential YoeB activation during heat shock in E. coli as previously demonstrated. These data provide a molecular basis for the ability of YoeB to function in response to thermal stress.
- Authors:
-
- Emory Univ. School of Medicine, Atlanta, GA (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Org.:
- USDOE Office of Science (SC); National Institutes of Health (NIH)
- OSTI Identifier:
- 1573380
- Grant/Contract Number:
- AC02-06CH11357; R01GM093278; GM103403; S10 RR029205
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nucleic Acids Research
- Additional Journal Information:
- Journal Volume: 47; Journal Issue: 19; Journal ID: ISSN 0305-1048
- Publisher:
- Oxford University Press
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Pavelich, Ian J., Maehigashi, Tatsuya, Hoffer, Eric D., Ruangprasert, Ajchareeya, Miles, Stacey J., and Dunham, Christine M. Monomeric YoeB toxin retains RNase activity but adopts an obligate dimeric form for thermal stability. United States: N. p., 2019.
Web. doi:10.1093/nar/gkz760.
Pavelich, Ian J., Maehigashi, Tatsuya, Hoffer, Eric D., Ruangprasert, Ajchareeya, Miles, Stacey J., & Dunham, Christine M. Monomeric YoeB toxin retains RNase activity but adopts an obligate dimeric form for thermal stability. United States. https://doi.org/10.1093/nar/gkz760
Pavelich, Ian J., Maehigashi, Tatsuya, Hoffer, Eric D., Ruangprasert, Ajchareeya, Miles, Stacey J., and Dunham, Christine M. Tue .
"Monomeric YoeB toxin retains RNase activity but adopts an obligate dimeric form for thermal stability". United States. https://doi.org/10.1093/nar/gkz760. https://www.osti.gov/servlets/purl/1573380.
@article{osti_1573380,
title = {Monomeric YoeB toxin retains RNase activity but adopts an obligate dimeric form for thermal stability},
author = {Pavelich, Ian J. and Maehigashi, Tatsuya and Hoffer, Eric D. and Ruangprasert, Ajchareeya and Miles, Stacey J. and Dunham, Christine M.},
abstractNote = {Chromosomally-encoded toxin-antitoxin complexes are ubiquitous in bacteria and regulate growth through the release of the toxin component typically in a stress-dependent manner. Type II ribosome-dependent toxins adopt a RelE-family RNase fold and inhibit translation by degrading mRNAs while bound to the ribosome. Here, we present biochemical and structural studies of the Escherichia coli YoeB toxin interacting with both a UAA stop and an AAU sense codon in pre- and post-mRNA cleavage states to provide insights into possible mRNA substrate selection. Both mRNAs undergo minimal changes during the cleavage event in contrast to type II ribosome-dependent RelE toxin. Further, the 16S rRNA decoding site nucleotides that monitor the mRNA in the aminoacyl(A) site adopt different orientations depending upon which toxin is present. Although YoeB is a RelE family member, it is the sole ribosome-dependent toxin that is dimeric. We show that engineered monomeric YoeB is active against mRNAs bound to both the small and large subunit. However, the stability of monomeric YoeB is reduced ~20°C, consistent with potential YoeB activation during heat shock in E. coli as previously demonstrated. These data provide a molecular basis for the ability of YoeB to function in response to thermal stress.},
doi = {10.1093/nar/gkz760},
journal = {Nucleic Acids Research},
number = 19,
volume = 47,
place = {United States},
year = {Tue Sep 10 00:00:00 EDT 2019},
month = {Tue Sep 10 00:00:00 EDT 2019}
}
Web of Science
Works referenced in this record:
Allostery and Intrinsic Disorder Mediate Transcription Regulation by Conditional Cooperativity
journal, July 2010
- Garcia-Pino, Abel; Balasubramanian, Sreeram; Wyns, Lode
- Cell, Vol. 142, Issue 1
Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes
journal, February 2005
- Pandey, D. P.
- Nucleic Acids Research, Vol. 33, Issue 3
Bacterial toxin YafQ is an endoribonuclease that associates with the ribosome and blocks translation elongation through sequence-specific and frame-dependent mRNA cleavage
journal, March 2009
- Prysak, Meredith H.; Mozdzierz, Christopher J.; Cook, Angela M.
- Molecular Microbiology, Vol. 71, Issue 5
A Salmonella Toxin Promotes Persister Formation through Acetylation of tRNA
journal, July 2016
- Cheverton, Angela M.; Gollan, Bridget; Przydacz, Michal
- Molecular Cell, Vol. 63, Issue 1
Mechanism of endonuclease cleavage by the HigB toxin
journal, July 2016
- Schureck, Marc A.; Repack, Adrienne; Miles, Stacey J.
- Nucleic Acids Research, Vol. 44, Issue 16
Crystal structure of the 30 S ribosomal subunit from Thermus thermophilus: purification, crystallization and structure determination
journal, July 2001
- Clemons, William M.; Brodersen, Ditlev E.; McCutcheon, John P.
- Journal of Molecular Biology, Vol. 310, Issue 4
Structural aspects of messenger RNA reading frame maintenance by the ribosome
journal, April 2010
- Jenner, Lasse B.; Demeshkina, Natalia; Yusupova, Gulnara
- Nature Structural & Molecular Biology, Vol. 17, Issue 5
Unique type of plasmid maintenance function: postsegregational killing of plasmid-free cells.
journal, May 1986
- Gerdes, K.; Rasmussen, P. B.; Molin, S.
- Proceedings of the National Academy of Sciences, Vol. 83, Issue 10
Unexpected Electrophoretic Migration of RNA with Different 3‘ Termini Causes a RNA Sizing Ambiguity That Can Be Resolved Using Nuclease P1-Generated Sequencing Ladders †
journal, April 1998
- Cruz-Reyes, Jorge; Piller, Kenneth J.; Rusché,, Laura N.
- Biochemistry, Vol. 37, Issue 17
The structure and function of Mycobacterium tuberculosis MazF-mt6 toxin provide insights into conserved features of MazF endonucleases
journal, March 2017
- Hoffer, Eric D.; Miles, Stacey J.; Dunham, Christine M.
- Journal of Biological Chemistry, Vol. 292, Issue 19
Epistasis analysis of 16S rRNA ram mutations helps define the conformational dynamics of the ribosome that influence decoding
journal, February 2016
- Ying, Lanqing; Fredrick, Kurt
- RNA, Vol. 22, Issue 4
RelE, a global inhibitor of translation, is activated during nutritional stress
journal, November 2001
- Christensen, S. K.; Mikkelsen, M.; Pedersen, K.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 25
Bacterial Toxin HigB Associates with Ribosomes and Mediates Translation-dependent mRNA Cleavage at A-rich Sites
journal, May 2009
- Hurley, Jennifer M.; Woychik, Nancy A.
- Journal of Biological Chemistry, Vol. 284, Issue 28
YoeB toxin is activated during thermal stress
journal, July 2015
- Janssen, Brian D.; Garza-Sánchez, Fernando; Hayes, Christopher S.
- MicrobiologyOpen, Vol. 4, Issue 4
Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses
journal, January 2010
- Christensen-Dalsgaard, Mikkel; Jørgensen, Mikkel Girke; Gerdes, Kenn
- Molecular Microbiology, Vol. 75, Issue 2
The Structural Basis for mRNA Recognition and Cleavage by the Ribosome-Dependent Endonuclease RelE
journal, December 2009
- Neubauer, Cajetan; Gao, Yong-Gui; Andersen, Kasper R.
- Cell, Vol. 139, Issue 6
Bacterial lifestyle shapes stringent response activation
journal, April 2013
- Boutte, Cara C.; Crosson, Sean
- Trends in Microbiology, Vol. 21, Issue 4
VapC20 of Mycobacterium tuberculosis cleaves the Sarcin–Ricin loop of 23S rRNA
journal, November 2013
- Winther, Kristoffer S.; Brodersen, Ditlev E.; Brown, Alistair K.
- Nature Communications, Vol. 4, Issue 1
The bacterial translation stress response
journal, November 2014
- Starosta, Agata L.; Lassak, Jürgen; Jung, Kirsten
- FEMS Microbiology Reviews, Vol. 38, Issue 6
Overproduction of the Lon protease triggers inhibition of translation in Escherichia coli: involvement of the yefM-yoeB toxin-antitoxin system: Lon-dependent translation inhibition
journal, February 2004
- Christensen, Susanne K.; Maenhaut-Michel, Geneviève; Mine, Natacha
- Molecular Microbiology, Vol. 51, Issue 6
The Inhibitory Mechanism of Protein Synthesis by YoeB, an Escherichia coli Toxin
journal, January 2009
- Zhang, Yonglong; Inouye, Masayori
- Journal of Biological Chemistry, Vol. 284, Issue 11
Escherichia coli ItaT is a type II toxin that inhibits translation by acetylating isoleucyl-tRNAIle
journal, June 2018
- Wilcox, Brendan; Osterman, Ilya; Serebryakova, Marina
- Nucleic Acids Research, Vol. 46, Issue 15
Translation affects YoeB and MazF messenger RNA interferase activities by different mechanisms
journal, October 2008
- Christensen-Dalsgaard, M.; Gerdes, K.
- Nucleic Acids Research, Vol. 36, Issue 20
The ratio between CcdA and CcdB modulates the transcriptional repression of the ccd poison-antidote system: Autoregulation of the ccd operon
journal, July 2001
- Afif, Hassan; Allali, Noureddine; Couturier, Martine
- Molecular Microbiology, Vol. 41, Issue 1
A New Plasmid-Encoded Proteic Killer Gene System: Cloning, Sequencing, and AnalyzinghigLocus of Plasmid Rts1
journal, March 1996
- Tian, Qing Bao; Ohnishi, Makoto; Tabuchi, Akira
- Biochemical and Biophysical Research Communications, Vol. 220, Issue 2
Structure of the 30S ribosomal subunit
journal, September 2000
- Wimberly, Brian T.; Brodersen, Ditlev E.; Clemons, William M.
- Nature, Vol. 407, Issue 6802
Ribosomal ambiguity ( ram ) mutations promote the open (off) to closed (on) transition and thereby increase miscoding
journal, November 2018
- Hoffer, Eric D.; Maehigashi, Tatsuya; Fredrick, Kurt
- Nucleic Acids Research, Vol. 47, Issue 3
RelE toxins from Bacteria and Archaea cleave mRNAs on translating ribosomes, which are rescued by tmRNA: tmRNA counteracts RelE
journal, May 2003
- Christensen, Susanne K.; Gerdes, Kenn
- Molecular Microbiology, Vol. 48, Issue 5
Insights into the mRNA Cleavage Mechanism by MazF, an mRNA Interferase
journal, November 2004
- Zhang, Yonglong; Zhang, Junjie; Hara, Hiroto
- Journal of Biological Chemistry, Vol. 280, Issue 5
Enteric virulence associated protein VapC inhibits translation by cleavage of initiator tRNA
journal, April 2011
- Winther, K. S.; Gerdes, K.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 18
Toxins, Targets, and Triggers: An Overview of Toxin-Antitoxin Biology
journal, June 2018
- Harms, Alexander; Brodersen, Ditlev Egeskov; Mitarai, Namiko
- Molecular Cell, Vol. 70, Issue 5
Toxin-antitoxin systems in bacterial growth arrest and persistence
journal, March 2016
- Page, Rebecca; Peti, Wolfgang
- Nature Chemical Biology, Vol. 12, Issue 4
Toxin-Antitoxin Systems Are Important for Niche-Specific Colonization and Stress Resistance of Uropathogenic Escherichia coli
journal, October 2012
- Norton, J. Paul; Mulvey, Matthew A.
- PLoS Pathogens, Vol. 8, Issue 10
ParE toxin encoded by the broad-host-range plasmid RK2 is an inhibitor of Escherichia coli gyrase: Inhibitory activity of ParE toxin
journal, May 2002
- Jiang, Yong; Pogliano, Joe; Helinski, Donald R.
- Molecular Microbiology, Vol. 44, Issue 4
Internalization of Salmonella by Macrophages Induces Formation of Nonreplicating Persisters
journal, January 2014
- Helaine, S.; Cheverton, A. M.; Watson, K. G.
- Science, Vol. 343, Issue 6167
Global Analysis of the E. coli Toxin MazF Reveals Widespread Cleavage of mRNA and the Inhibition of rRNA Maturation and Ribosome Biogenesis
journal, June 2018
- Culviner, Peter H.; Laub, Michael T.
- Molecular Cell, Vol. 70, Issue 5
Bacterial Toxin RelE Mediates Frequent Codon-independent mRNA Cleavage from the 5′ End of Coding Regions in Vivo
journal, February 2011
- Hurley, Jennifer M.; Cruz, Jonathan W.; Ouyang, Ming
- Journal of Biological Chemistry, Vol. 286, Issue 17
mRNA bound to the 30S subunit is a HigB toxin substrate
journal, June 2016
- Schureck, Marc A.; Maehigashi, Tatsuya; Miles, Stacey J.
- RNA, Vol. 22, Issue 8
Molecular Mechanism of Bacterial Persistence by HipA
journal, October 2013
- Germain, Elsa; Castro-Roa, Daniel; Zenkin, Nikolay
- Molecular Cell, Vol. 52, Issue 2
The Bacterial Toxin RelE Displays Codon-Specific Cleavage of mRNAs in the Ribosomal A Site
journal, January 2003
- Pedersen, Kim; Zavialov, Andrey V.; Pavlov, Michael Yu.
- Cell, Vol. 112, Issue 1
Structure of the Proteus vulgaris HigB-(HigA) 2 -HigB Toxin-Antitoxin Complex
journal, November 2013
- Schureck, Marc A.; Maehigashi, Tatsuya; Miles, Stacey J.
- Journal of Biological Chemistry, Vol. 289, Issue 2
Mycobacterial toxin MazF-mt6 inhibits translation through cleavage of 23S rRNA at the ribosomal A site
journal, May 2013
- Schifano, J. M.; Edifor, R.; Sharp, J. D.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 21
Messenger RNA interferase RelE controls relBE transcription by conditional cooperativity
journal, August 2008
- Overgaard, Martin; Borch, Jonas; Jørgensen, Mikkel G.
- Molecular Microbiology, Vol. 69, Issue 4
DNA Damage Responses in Prokaryotes: Regulating Gene Expression, Modulating Growth Patterns, and Manipulating Replication Forks
journal, October 2013
- Kreuzer, K. N.
- Cold Spring Harbor Perspectives in Biology, Vol. 5, Issue 11
Molecular basis of ribosome recognition and mRNA hydrolysis by the E. coli YafQ toxin
journal, August 2015
- Maehigashi, Tatsuya; Ruangprasert, Ajchareeya; Miles, Stacey J.
- Nucleic Acids Research, Vol. 43, Issue 16
Conformational Change in the Catalytic Site of the Ribonuclease YoeB Toxin by YefM Antitoxin
journal, August 2005
- Kamada, Katsuhiko; Hanaoka, Fumio
- Molecular Cell, Vol. 19, Issue 4
The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu
journal, October 2013
- Castro-Roa, Daniel; Garcia-Pino, Abel; De Gieter, Steven
- Nature Chemical Biology, Vol. 9, Issue 12
YoeB–ribosome structure: a canonical RNase that requires the ribosome for its specific activity
journal, August 2013
- Feng, Shu; Chen, Yun; Kamada, Katsuhiko
- Nucleic Acids Research, Vol. 41, Issue 20
An RNA-seq method for defining endoribonuclease cleavage specificity identifies dual rRNA substrates for toxin MazF-mt3
journal, April 2014
- Schifano, Jason M.; Vvedenskaya, Irina O.; Knoblauch, Jared G.
- Nature Communications, Vol. 5, Issue 1
The Heat Shock Response: Life on the Verge of Death
journal, October 2010
- Richter, Klaus; Haslbeck, Martin; Buchner, Johannes
- Molecular Cell, Vol. 40, Issue 2
Structure of the 70S Ribosome Complexed with mRNA and tRNA
journal, September 2006
- Selmer, M.
- Science, Vol. 313, Issue 5795
F plasmid ccd mechanism in Escherichia coli.
journal, April 1986
- Hiraga, S.; Jaffé, A.; Ogura, T.
- Journal of Bacteriology, Vol. 166, Issue 1
Defining the mRNA recognition signature of a bacterial toxin protein
journal, October 2015
- Schureck, Marc A.; Dunkle, Jack A.; Maehigashi, Tatsuya
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 45
Doc Toxin Is a Kinase That Inactivates Elongation Factor Tu
journal, January 2014
- Cruz, Jonathan W.; Rothenbacher, Francesca P.; Maehigashi, Tatsuya
- Journal of Biological Chemistry, Vol. 289, Issue 11
The Structural Basis for mRNA Recognition and Cleavage by the Ribosome-Dependent Endonuclease RelE
text, January 2009
- Neubauer, C.; Gao, Y. -G.; Andersen, K. R.
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu
text, January 2013
- Castro-Roa, Daniel; Garcia-Pino, Abel; De Gieter, Steven
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
YoeB toxin is activated during thermal stress
journal, July 2015
- Janssen, Brian D.; Garza-Sánchez, Fernando; Hayes, Christopher S.
- MicrobiologyOpen, Vol. 4, Issue 4
A New Plasmid-Encoded Proteic Killer Gene System: Cloning, Sequencing, and AnalyzinghigLocus of Plasmid Rts1
journal, March 1996
- Tian, Qing Bao; Ohnishi, Makoto; Tabuchi, Akira
- Biochemical and Biophysical Research Communications, Vol. 220, Issue 2
Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes
journal, August 1992
- Bernard, Philippe; Couturier, Martine
- Journal of Molecular Biology, Vol. 226, Issue 3
Allostery and Intrinsic Disorder Mediate Transcription Regulation by Conditional Cooperativity
journal, July 2010
- Garcia-Pino, Abel; Balasubramanian, Sreeram; Wyns, Lode
- Cell, Vol. 142, Issue 1
Conformational Change in the Catalytic Site of the Ribonuclease YoeB Toxin by YefM Antitoxin
journal, August 2005
- Kamada, Katsuhiko; Hanaoka, Fumio
- Molecular Cell, Vol. 19, Issue 4
The Heat Shock Response: Life on the Verge of Death
journal, October 2010
- Richter, Klaus; Haslbeck, Martin; Buchner, Johannes
- Molecular Cell, Vol. 40, Issue 2
Molecular Mechanism of Bacterial Persistence by HipA
journal, October 2013
- Germain, Elsa; Castro-Roa, Daniel; Zenkin, Nikolay
- Molecular Cell, Vol. 52, Issue 2
Toxins, Targets, and Triggers: An Overview of Toxin-Antitoxin Biology
journal, June 2018
- Harms, Alexander; Brodersen, Ditlev Egeskov; Mitarai, Namiko
- Molecular Cell, Vol. 70, Issue 5
Selection of tRNA by the Ribosome Requires a Transition from an Open to a Closed Form
journal, November 2002
- Ogle, James M.; Murphy, Frank V.; Tarry, Michael J.
- Cell, Vol. 111, Issue 5
MazF Cleaves Cellular mRNAs Specifically at ACA to Block Protein Synthesis in Escherichia coli
journal, October 2003
- Zhang, Yonglong; Zhang, Junjie; Hoeflich, Klaus P.
- Molecular Cell, Vol. 12, Issue 4
Toxin-antitoxin systems in bacterial growth arrest and persistence
journal, March 2016
- Page, Rebecca; Peti, Wolfgang
- Nature Chemical Biology, Vol. 12, Issue 4
VapC20 of Mycobacterium tuberculosis cleaves the Sarcin–Ricin loop of 23S rRNA
journal, November 2013
- Winther, Kristoffer S.; Brodersen, Ditlev E.; Brown, Alistair K.
- Nature Communications, Vol. 4, Issue 1
An RNA-seq method for defining endoribonuclease cleavage specificity identifies dual rRNA substrates for toxin MazF-mt3
journal, April 2014
- Schifano, Jason M.; Vvedenskaya, Irina O.; Knoblauch, Jared G.
- Nature Communications, Vol. 5, Issue 1
Structural aspects of messenger RNA reading frame maintenance by the ribosome
journal, April 2010
- Jenner, Lasse B.; Demeshkina, Natalia; Yusupova, Gulnara
- Nature Structural & Molecular Biology, Vol. 17, Issue 5
Enteric virulence associated protein VapC inhibits translation by cleavage of initiator tRNA
journal, April 2011
- Winther, K. S.; Gerdes, K.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 18
Mycobacterial toxin MazF-mt6 inhibits translation through cleavage of 23S rRNA at the ribosomal A site
journal, May 2013
- Schifano, J. M.; Edifor, R.; Sharp, J. D.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 21
RelE, a global inhibitor of translation, is activated during nutritional stress
journal, November 2001
- Christensen, S. K.; Mikkelsen, M.; Pedersen, K.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 25
Unique type of plasmid maintenance function: postsegregational killing of plasmid-free cells.
journal, May 1986
- Gerdes, K.; Rasmussen, P. B.; Molin, S.
- Proceedings of the National Academy of Sciences, Vol. 83, Issue 10
Doc toxin is a kinase that inactivates elongation factor Tu.
journal, July 2014
- Cruz, Jonathan W.; Rothenbacher, Francesca P.; Maehigashi, Tatsuya
- Journal of Biological Chemistry, Vol. 289, Issue 28
Molecular Structure and Function of the Novel BrnT/BrnA Toxin-Antitoxin System of Brucella abortus
journal, February 2012
- Heaton, Brook E.; Herrou, Julien; Blackwell, Anne E.
- Journal of Biological Chemistry, Vol. 287, Issue 15
Mechanisms of Toxin Inhibition and Transcriptional Repression by Escherichia coli DinJ-YafQ
journal, June 2014
- Ruangprasert, Ajchareeya; Maehigashi, Tatsuya; Miles, Stacey J.
- Journal of Biological Chemistry, Vol. 289, Issue 30
Translation affects YoeB and MazF messenger RNA interferase activities by different mechanisms
journal, October 2008
- Christensen-Dalsgaard, M.; Gerdes, K.
- Nucleic Acids Research, Vol. 36, Issue 20
YoeB–ribosome structure: a canonical RNase that requires the ribosome for its specific activity
journal, August 2013
- Feng, Shu; Chen, Yun; Kamada, Katsuhiko
- Nucleic Acids Research, Vol. 41, Issue 20
Molecular basis of ribosome recognition and mRNA hydrolysis by the E. coli YafQ toxin
journal, August 2015
- Maehigashi, Tatsuya; Ruangprasert, Ajchareeya; Miles, Stacey J.
- Nucleic Acids Research, Vol. 43, Issue 16
Mechanism of endonuclease cleavage by the HigB toxin
journal, July 2016
- Schureck, Marc A.; Repack, Adrienne; Miles, Stacey J.
- Nucleic Acids Research, Vol. 44, Issue 16
Escherichia coli ItaT is a type II toxin that inhibits translation by acetylating isoleucyl-tRNAIle
journal, June 2018
- Wilcox, Brendan; Osterman, Ilya; Serebryakova, Marina
- Nucleic Acids Research, Vol. 46, Issue 15
The bacterial translation stress response
journal, November 2014
- Starosta, Agata L.; Lassak, Jürgen; Jung, Kirsten
- FEMS Microbiology Reviews, Vol. 38, Issue 6
Messenger RNA interferase RelE controls relBE transcription by conditional cooperativity
journal, August 2008
- Overgaard, Martin; Borch, Jonas; Jørgensen, Mikkel G.
- Molecular Microbiology, Vol. 69, Issue 4
Bacterial toxin YafQ is an endoribonuclease that associates with the ribosome and blocks translation elongation through sequence-specific and frame-dependent mRNA cleavage
journal, March 2009
- Prysak, Meredith H.; Mozdzierz, Christopher J.; Cook, Angela M.
- Molecular Microbiology, Vol. 71, Issue 5
Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses
journal, January 2010
- Christensen-Dalsgaard, Mikkel; Jørgensen, Mikkel Girke; Gerdes, Kenn
- Molecular Microbiology, Vol. 75, Issue 2
Structure of the 70S Ribosome Complexed with mRNA and tRNA
journal, September 2006
- Selmer, M.
- Science, Vol. 313, Issue 5795
HicA of Escherichia coli Defines a Novel Family of Translation-Independent mRNA Interferases in Bacteria and Archaea
journal, December 2008
- Jorgensen, M. G.; Pandey, D. P.; Jaskolska, M.
- Journal of Bacteriology, Vol. 191, Issue 4
F plasmid ccd mechanism in Escherichia coli.
journal, April 1986
- Hiraga, S.; Jaffé, A.; Ogura, T.
- Journal of Bacteriology, Vol. 166, Issue 1
Epistasis analysis of 16S rRNA ram mutations helps define the conformational dynamics of the ribosome that influence decoding
journal, February 2016
- Ying, Lanqing; Fredrick, Kurt
- RNA, Vol. 22, Issue 4
mRNA bound to the 30S subunit is a HigB toxin substrate
journal, June 2016
- Schureck, Marc A.; Maehigashi, Tatsuya; Miles, Stacey J.
- RNA, Vol. 22, Issue 8
The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu
text, January 2013
- Castro-Roa, Daniel; Garcia-Pino, Abel; De Gieter, Steven
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Works referencing / citing this record:
Identifying a Molecular Mechanism That Imparts Species-Specific Toxicity to YoeB Toxins
journal, May 2020
- Ames, Jessica R.; McGillick, Julia; Murphy, Tamiko
- Frontiers in Microbiology, Vol. 11
Functional Characterization of the mazEF Toxin-Antitoxin System in the Pathogenic Bacterium Agrobacterium tumefaciens
journal, May 2021
- Choi, Wonho; Yamaguchi, Yoshihiro; Park, Ji-Young
- Microorganisms, Vol. 9, Issue 5