Delignification of miscanthus using ethylenediamine (EDA) with or without ammonia and subsequent enzymatic hydrolysis to sugars
Abstract
Pretreatment of miscanthus is essential for efficient enzymatic production of cellulosic ethanol. This study reports a possible pretreatment method for miscanthus using aqueous ethylenediamine (EDA) for 30 min at 180 °C with or without ammonia. The mass ratio of miscanthus to EDA was varied from 1:3, 1:1, and 1:0.5, keeping the mass ratio of miscanthus to liquid (EDA + Water) constant at 1:8. The ammonia-to-miscanthus ratio was 1:0.25. After pretreatment with a ratio of 1:3 miscanthus to EDA, about 75 % of the lignin was removed from the raw miscanthus with 90 % retention of cellulose and 50 % of hemicellulose in the recovered solid. Enzymatic hydrolysis of the recovered solid miscanthus gave 63 % glucose and 62 % xylose conversion after 72 h. EDA provides an effective pretreatment for miscanthus, achieving good delignification and enhanced sugar yield by enzyme hydrolysis. Results using aqueous EDA with or without ammonia are much better than those using hot water and compare favorably with those using aqueous ammonia. The delignification efficiency of EDA pretreatment is high compared to that for hot-water pretreatment and is nearly as efficient as that obtained for aqueous-ammonia pretreatment.
- Authors:
-
- Univ. of California, Berkeley, CA (United States); Praj Industries, Pune (India)
- Univ. of California, Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1571069
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- 3 Biotech
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 1; Journal ID: ISSN 2190-572X
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Padmanabhan, Sasisanker, Schwyter, Philippe, Liu, Zhongguo, Poon, Geoffrey, Bell, Alexis T., and Prausnitz, John M. Delignification of miscanthus using ethylenediamine (EDA) with or without ammonia and subsequent enzymatic hydrolysis to sugars. United States: N. p., 2016.
Web. doi:10.1007/s13205-015-0344-z.
Padmanabhan, Sasisanker, Schwyter, Philippe, Liu, Zhongguo, Poon, Geoffrey, Bell, Alexis T., & Prausnitz, John M. Delignification of miscanthus using ethylenediamine (EDA) with or without ammonia and subsequent enzymatic hydrolysis to sugars. United States. https://doi.org/10.1007/s13205-015-0344-z
Padmanabhan, Sasisanker, Schwyter, Philippe, Liu, Zhongguo, Poon, Geoffrey, Bell, Alexis T., and Prausnitz, John M. Mon .
"Delignification of miscanthus using ethylenediamine (EDA) with or without ammonia and subsequent enzymatic hydrolysis to sugars". United States. https://doi.org/10.1007/s13205-015-0344-z. https://www.osti.gov/servlets/purl/1571069.
@article{osti_1571069,
title = {Delignification of miscanthus using ethylenediamine (EDA) with or without ammonia and subsequent enzymatic hydrolysis to sugars},
author = {Padmanabhan, Sasisanker and Schwyter, Philippe and Liu, Zhongguo and Poon, Geoffrey and Bell, Alexis T. and Prausnitz, John M.},
abstractNote = {Pretreatment of miscanthus is essential for efficient enzymatic production of cellulosic ethanol. This study reports a possible pretreatment method for miscanthus using aqueous ethylenediamine (EDA) for 30 min at 180 °C with or without ammonia. The mass ratio of miscanthus to EDA was varied from 1:3, 1:1, and 1:0.5, keeping the mass ratio of miscanthus to liquid (EDA + Water) constant at 1:8. The ammonia-to-miscanthus ratio was 1:0.25. After pretreatment with a ratio of 1:3 miscanthus to EDA, about 75 % of the lignin was removed from the raw miscanthus with 90 % retention of cellulose and 50 % of hemicellulose in the recovered solid. Enzymatic hydrolysis of the recovered solid miscanthus gave 63 % glucose and 62 % xylose conversion after 72 h. EDA provides an effective pretreatment for miscanthus, achieving good delignification and enhanced sugar yield by enzyme hydrolysis. Results using aqueous EDA with or without ammonia are much better than those using hot water and compare favorably with those using aqueous ammonia. The delignification efficiency of EDA pretreatment is high compared to that for hot-water pretreatment and is nearly as efficient as that obtained for aqueous-ammonia pretreatment.},
doi = {10.1007/s13205-015-0344-z},
journal = {3 Biotech},
number = 1,
volume = 6,
place = {United States},
year = {Mon Jan 11 00:00:00 EST 2016},
month = {Mon Jan 11 00:00:00 EST 2016}
}
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Works referenced in this record:
Solubility and rate of dissolution for Miscanthus in hydrophilic ionic liquids
journal, October 2011
- Padmanabhan, Sasisanker; Kim, Michael; Blanch, Harvey W.
- Fluid Phase Equilibria, Vol. 309, Issue 1
Efficient hydrogen production from the lignocellulosic energy crop Miscanthus by the extreme thermophilic bacteria Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana
journal, January 2009
- de Vrije, Truus; Bakker, Robert R.; Budde, Miriam AW
- Biotechnology for Biofuels, Vol. 2, Issue 1
Efficient hydrogen production from the lignocellulosic energy crop Miscanthus by the extreme thermophilic bacteria Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana
journal, January 2009
- de Vrije, Truus; Bakker, Robert R.; Budde, Miriam AW
- Biotechnology for Biofuels, Vol. 2, Issue 1
Solubility and rate of dissolution for Miscanthus in hydrophilic ionic liquids
journal, October 2011
- Padmanabhan, Sasisanker; Kim, Michael; Blanch, Harvey W.
- Fluid Phase Equilibria, Vol. 309, Issue 1
Advances in ethanol production
journal, June 2011
- Geddes, Claudia C.; Nieves, Ismael U.; Ingram, Lonnie O.
- Current Opinion in Biotechnology, Vol. 22, Issue 3
Delignification of Miscanthus by Extraction
journal, January 2012
- Padmanabhan, Sasisanker; Zaia, Edmond; Wu, Katherine
- Separation Science and Technology, Vol. 47, Issue 2
Biomass deconstruction to sugars
journal, August 2011
- Blanch, Harvey W.; Simmons, Blake A.; Klein-Marcuschamer, Daniel
- Biotechnology Journal, Vol. 6, Issue 9
Pretreatment of rice straw with ammonia and ionic liquid for lignocellulose conversion to fermentable sugars
journal, October 2010
- Nguyen, Tam-Anh D.; Kim, Kyoung-Rok; Han, Se Jong
- Bioresource Technology, Vol. 101, Issue 19
Advances in ethanol production
journal, June 2011
- Geddes, Claudia C.; Nieves, Ismael U.; Ingram, Lonnie O.
- Current Opinion in Biotechnology, Vol. 22, Issue 3
Comparison of different pretreatment methods for lignocellulosic materials. Part I: Conversion of rye straw to valuable products
journal, April 2011
- Ingram, Thomas; Wörmeyer, Kai; Lima, Juan Carlos Ixcaraguá
- Bioresource Technology, Vol. 102, Issue 8
‘Cradle-to-grave’ assessment of existing lignocellulose pretreatment technologies
journal, June 2009
- da Costa Sousa, Leonardo; Chundawat, Shishir PS; Balan, Venkatesh
- Current Opinion in Biotechnology, Vol. 20, Issue 3
Comparative sugar recovery and fermentation data following pretreatment of poplar wood by leading technologies
journal, March 2009
- Wyman, Charles E.; Dale, Bruce E.; Elander, Richard T.
- Biotechnology Progress, Vol. 25, Issue 2
'Cradle-to-grave' assessment of existing lignocellulose pretreatment technologies
text, January 2009
- Sousa, Leonardo; Chundawat, Shishir P. S.; Balan, Venkatesh
- Rutgers University
Pretreatment of rice straw with ammonia and ionic liquid for lignocellulose conversion to fermentable sugars
journal, October 2010
- Nguyen, Tam-Anh D.; Kim, Kyoung-Rok; Han, Se Jong
- Bioresource Technology, Vol. 101, Issue 19
Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources
journal, December 2011
- Wyman, Charles E.; Balan, Venkatesh; Dale, Bruce E.
- Bioresource Technology, Vol. 102, Issue 24
Comparison of different pretreatment methods for lignocellulosic materials. Part I: Conversion of rye straw to valuable products
journal, April 2011
- Ingram, Thomas; Wörmeyer, Kai; Lima, Juan Carlos Ixcaraguá
- Bioresource Technology, Vol. 102, Issue 8
Effect of enzyme supplementation at moderate cellulase loadings on initial glucose and xylose release from corn stover solids pretreated by leading technologies
journal, February 2009
- Kumar, Rajeev; Wyman, C. E.
- Biotechnology and Bioengineering, Vol. 102, Issue 2, p. 457-467
Dissolution of Ethylenediamine Pretreated Pulp with High Lignin Content in LiCl/DMSO without Milling
journal, August 2010
- Wang, Zhiguo; Yokoyama, Tomoya; Matsumoto, Yuji
- Journal of Wood Chemistry and Technology, Vol. 30, Issue 3
Miscanthus : a fast-growing crop for biofuels and chemicals production : A fast-growing crop for biofuels and chemicals production
journal, July 2012
- Brosse, Nicolas; Dufour, Anthony; Meng, Xianzhi
- Biofuels, Bioproducts and Biorefining, Vol. 6, Issue 5
Biomass deconstruction to sugars
journal, August 2011
- Blanch, Harvey W.; Simmons, Blake A.; Klein-Marcuschamer, Daniel
- Biotechnology Journal, Vol. 6, Issue 9
Ethanol production from rice straw using optimized aqueous-ammonia soaking pretreatment and simultaneous saccharification and fermentation processes
journal, October 2009
- Ko, Ja Kyong; Bak, Jin Seop; Jung, Min Woo
- Bioresource Technology, Vol. 100, Issue 19
Pretreatment of corn stover by combining ionic liquid dissolution with alkali extraction
journal, August 2011
- Geng, Xinglian; Henderson, Wesley A.
- Biotechnology and Bioengineering, Vol. 109, Issue 1
Dissolution of Ethylenediamine Pretreated Pulp with High Lignin Content in LiCl/DMSO without Milling
journal, August 2010
- Wang, Zhiguo; Yokoyama, Tomoya; Matsumoto, Yuji
- Journal of Wood Chemistry and Technology, Vol. 30, Issue 3
Compositional Analysis of Lignocellulosic Feedstocks. 1. Review and Description of Methods
journal, August 2010
- Sluiter, Justin B.; Ruiz, Raymond O.; Scarlata, Christopher J.
- Journal of Agricultural and Food Chemistry, Vol. 58, Issue 16, p. 9043-9053
Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources
journal, December 2011
- Wyman, Charles E.; Balan, Venkatesh; Dale, Bruce E.
- Bioresource Technology, Vol. 102, Issue 24
Comparison of the effects of five pretreatment methods on enhancing the enzymatic digestibility and ethanol production from sweet sorghum bagasse
journal, May 2012
- Cao, Weixing; Sun, Chen; Liu, Ronghou
- Bioresource Technology, Vol. 111
Measurement of cellulase activities
journal, January 1987
- Ghose, T. K.
- Pure and Applied Chemistry, Vol. 59, Issue 2
Comparative study on enzymatic digestibility of switchgrass varieties and harvests processed by leading pretreatment technologies
journal, December 2011
- Kim, Youngmi; Mosier, Nathan S.; Ladisch, Michael R.
- Bioresource Technology, Vol. 102, Issue 24
Characterization of milled wood lignin and ethanol organosolv lignin from miscanthus
journal, October 2009
- El Hage, Roland; Brosse, Nicolas; Chrusciel, Laurent
- Polymer Degradation and Stability, Vol. 94, Issue 10
Release of monomeric sugars from Miscanthus sinensis by microwave-assisted ammonia and phosphoric acid treatments
journal, January 2012
- Boonmanumsin, P.; Treeboobpha, S.; Jeamjumnunja, K.
- Bioresource Technology, Vol. 103, Issue 1
Changes of wood properties treated with aqueous amine solution, bending tests and X-ray analysis of wood after amine treatment
journal, January 2011
- Ishikura, Yukiko
- Journal of Materials Science, Vol. 46, Issue 11
Technoeconomic analysis of biofuels: A wiki-based platform for lignocellulosic biorefineries
journal, December 2010
- Klein-Marcuschamer, Daniel; Oleskowicz-Popiel, Piotr; Simmons, Blake A.
- Biomass and Bioenergy, Vol. 34, Issue 12
Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review
journal, July 2010
- Alvira, P.; Tomás-Pejó, E.; Ballesteros, M.
- Bioresource Technology, Vol. 101, Issue 13, p. 4851-4861
Downscaled method using glass microfiber filters for the determination of Klason lignin and structural carbohydrates
journal, September 2014
- Ibáñez, Ana B.; Bauer, Stefan
- Biomass and Bioenergy, Vol. 68
Cellulosic Biofuels
journal, June 2009
- Carroll, Andrew; Somerville, Chris
- Annual Review of Plant Biology, Vol. 60, Issue 1
Biomass pretreatment: Fundamentals toward application
journal, November 2011
- Agbor, Valery B.; Cicek, Nazim; Sparling, Richard
- Biotechnology Advances, Vol. 29, Issue 6
Aqueous-ammonia delignification of miscanthus followed by enzymatic hydrolysis to sugars
journal, May 2013
- Liu, Zhongguo; Padmanabhan, Sasisanker; Cheng, Kun
- Bioresource Technology, Vol. 135
Addition of ammonia and/or oxygen to an ionic liquid for delignification of miscanthus
journal, September 2011
- Rodríguez, Héctor; Padmanabhan, Sasisanker; Poon, Geoffrey
- Bioresource Technology, Vol. 102, Issue 17
Effect of alkali pretreatment of wheat straw on the efficacy of exogenous fibrolytic enzymes1
journal, January 2004
- Wang, Y.; Spratling, B. M.; ZoBell, D. R.
- Journal of Animal Science, Vol. 82, Issue 1
Pretreatment with Ammonia Water for Enzymatic Hydrolysis of Corn Husk, Bagasse, and Switchgrass
journal, January 2001
- Kurakake, Masahiro; Kisaka, Wataru; Ouchi, Kazuya
- Applied Biochemistry and Biotechnology, Vol. 90, Issue 3
Supplementation with xylanase and β-xylosidase to reduce xylo-oligomer and xylan inhibition of enzymatic hydrolysis of cellulose and pretreated corn stover
journal, June 2011
- Qing, Qing; Wyman, Charles E.
- Biotechnology for Biofuels, Vol. 4, Issue 1, Article No: 18
Supplementation with xylanase and β-xylosidase to reduce xylo-oligomer and xylan inhibition of enzymatic hydrolysis of cellulose and pretreated corn stover
journal, June 2011
- Qing, Qing; Wyman, Charles E.
- Biotechnology for Biofuels, Vol. 4, Issue 1, Article No: 18
Comparison of different pretreatment methods for lignocellulosic materials. Part II: Influence of pretreatment on the properties of rye straw lignin
journal, March 2011
- Wörmeyer, Kai; Ingram, Thomas; Saake, Bodo
- Bioresource Technology, Vol. 102, Issue 5
Investigation of biomass degradation mechanism in pretreatment of switchgrass by aqueous ammonia and sodium hydroxide
journal, November 2010
- Gupta, Rajesh; Lee, Y. Y.
- Bioresource Technology, Vol. 101, Issue 21
Comparison of different pretreatment methods for lignocellulosic materials. Part II: Influence of pretreatment on the properties of rye straw lignin
journal, March 2011
- Wörmeyer, Kai; Ingram, Thomas; Saake, Bodo
- Bioresource Technology, Vol. 102, Issue 5
Technoeconomic analysis of biofuels: A wiki-based platform for lignocellulosic biorefineries
journal, December 2010
- Klein-Marcuschamer, Daniel; Oleskowicz-Popiel, Piotr; Simmons, Blake A.
- Biomass and Bioenergy, Vol. 34, Issue 12
Summary of findings from the Biomass Refining Consortium for Applied Fundamentals and Innovation (CAFI): corn stover pretreatment
journal, June 2009
- Elander, Richard T.; Dale, Bruce E.; Holtzapple, Mark
- Cellulose, Vol. 16, Issue 4
Changes of wood properties treated with aqueous amine solution, bending tests and X-ray analysis of wood after amine treatment
journal, January 2011
- Ishikura, Yukiko
- Journal of Materials Science, Vol. 46, Issue 11
Release of monomeric sugars from Miscanthus sinensis by microwave-assisted ammonia and phosphoric acid treatments
journal, January 2012
- Boonmanumsin, P.; Treeboobpha, S.; Jeamjumnunja, K.
- Bioresource Technology, Vol. 103, Issue 1
Ionic liquid pretreatment of cellulosic biomass: Enzymatic hydrolysis and ionic liquid recycle
journal, December 2010
- Shill, Kierston; Padmanabhan, Sasisanker; Xin, Qin
- Biotechnology and Bioengineering, Vol. 108, Issue 3, p. 511-520
Plant cell walls to ethanol
journal, February 2012
- Jordan, Douglas B.; Bowman, Michael J.; Braker, Jay D.
- Biochemical Journal, Vol. 442, Issue 2
Pretreatment of Miscanthus x giganteus Using the Ethanol Organosolv Process for Ethanol Production
journal, September 2009
- Brosse, Nicolas; Sannigrahi, Poulomi; Ragauskas, Arthur
- Industrial & Engineering Chemistry Research, Vol. 48, Issue 18
Cellulosic Biofuels
journal, June 2009
- Carroll, Andrew; Somerville, Chris
- Annual Review of Plant Biology, Vol. 60, Issue 1
Ethanol production from rice straw using optimized aqueous-ammonia soaking pretreatment and simultaneous saccharification and fermentation processes
journal, October 2009
- Ko, Ja Kyong; Bak, Jin Seop; Jung, Min Woo
- Bioresource Technology, Vol. 100, Issue 19
Process and technoeconomic analysis of leading pretreatment technologies for lignocellulosic ethanol production using switchgrass
journal, December 2011
- Tao, Ling; Aden, Andy; Elander, Richard T.
- Bioresource Technology, Vol. 102, Issue 24, p. 11105-11114
Comparison of the effects of five pretreatment methods on enhancing the enzymatic digestibility and ethanol production from sweet sorghum bagasse
journal, May 2012
- Cao, Weixing; Sun, Chen; Liu, Ronghou
- Bioresource Technology, Vol. 111
Compositional Analysis of Lignocellulosic Feedstocks. 2. Method Uncertainties
journal, August 2010
- Templeton, David W.; Scarlata, Christopher J.; Sluiter, Justin B.
- Journal of Agricultural and Food Chemistry, Vol. 58, Issue 16
Biomass pretreatment: Fundamentals toward application
journal, November 2011
- Agbor, Valery B.; Cicek, Nazim; Sparling, Richard
- Biotechnology Advances, Vol. 29, Issue 6
Aqueous-ammonia delignification of miscanthus followed by enzymatic hydrolysis to sugars
journal, May 2013
- Liu, Zhongguo; Padmanabhan, Sasisanker; Cheng, Kun
- Bioresource Technology, Vol. 135
Characterization of milled wood lignin and ethanol organosolv lignin from miscanthus
journal, October 2009
- El Hage, Roland; Brosse, Nicolas; Chrusciel, Laurent
- Polymer Degradation and Stability, Vol. 94, Issue 10
Sodium hydroxide pretreatment of genetically modified switchgrass for improved enzymatic release of sugars
journal, April 2012
- Wang, Ziyu; Li, Ruyu; Xu, Jiele
- Bioresource Technology, Vol. 110
Comparative study on enzymatic digestibility of switchgrass varieties and harvests processed by leading pretreatment technologies
journal, December 2011
- Kim, Youngmi; Mosier, Nathan S.; Ladisch, Michael R.
- Bioresource Technology, Vol. 102, Issue 24
Methods for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis and Biofuel Production
journal, April 2009
- Kumar, Parveen; Barrett, Diane M.; Delwiche, Michael J.
- Industrial & Engineering Chemistry Research, Vol. 48, Issue 8, p. 3713-3729
Delignification of Miscanthus by Extraction
journal, January 2012
- Padmanabhan, Sasisanker; Zaia, Edmond; Wu, Katherine
- Separation Science and Technology, Vol. 47, Issue 2
Pulping of Whole Jute Plant (Corchorus capsularies) by Soda-Amine Liquor
journal, October 2000
- Jahan, M. Sarwar; Farouqui, F. I.
- Holzforschung, Vol. 54, Issue 6
Investigation of biomass degradation mechanism in pretreatment of switchgrass by aqueous ammonia and sodium hydroxide
journal, November 2010
- Gupta, Rajesh; Lee, Y. Y.
- Bioresource Technology, Vol. 101, Issue 21
Effect of Urea Addition on Soda Pulping of Oak Wood
journal, February 2008
- Cho, Nam-Seok; Matsumoto, Yuji; Cho, Hee-Yeon
- Journal of the Faculty of Agriculture, Kyushu University, Vol. 53, Issue 1
Works referencing / citing this record:
Life cycle assessment of ethanol production from miscanthus: A comparison of production pathways at two European sites
journal, September 2018
- Lask, Jan; Wagner, Moritz; Trindade, Luisa M.
- GCB Bioenergy, Vol. 11, Issue 1