Structural characterization of Class 2 OLD family nucleases supports a two-metal catalysis mechanism for cleavage
Abstract
Overcoming lysogenization defect (OLD) proteins constitute a family of uncharacterized nucleases present in bacteria, archaea, and some viruses. These enzymes contain an N-terminal ATPase domain and a C-terminal Toprim domain common amongst replication, recombination, and repair proteins. The in vivo activities of OLD proteins remain poorly understood and no definitive structural information exists. Here we identify and define two classes of OLD proteins based on differences in gene neighborhood and amino acid sequence conservation and present the crystal structures of the catalytic C-terminal regions from the Burkholderia pseudomallei and Xanthamonas campestris p.v. campestris Class 2 OLD proteins at 2.24 Å and 1.86 Å resolution respectively. The structures reveal a two-domain architecture containing a Toprim domain with altered architecture and a unique helical domain. Conserved side chains contributed by both domains coordinate two bound magnesium ions in the active site of B. pseudomallei OLD in a geometry that supports a two-metal catalysis mechanism for cleavage. The spatial organization of these domains additionally suggests a novel mode of DNA binding that is distinct from other Toprim containing proteins. Together, these findings define the fundamental structural properties of the OLD family catalytic core and the underlying mechanism controlling nuclease activity.
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE
- Contributing Org.:
- Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
- OSTI Identifier:
- 1566816
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nucleic Acids Research
- Additional Journal Information:
- Journal Volume: 47; Journal Issue: 17; Journal ID: ISSN 0305-1048
- Publisher:
- Oxford University Press
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Schiltz, Carl J., Lee, April, Partlow, Edward A., Hosford, Christopher J., and Chappie, Joshua S. Structural characterization of Class 2 OLD family nucleases supports a two-metal catalysis mechanism for cleavage. United States: N. p., 2019.
Web. doi:10.1093/nar/gkz703.
Schiltz, Carl J., Lee, April, Partlow, Edward A., Hosford, Christopher J., & Chappie, Joshua S. Structural characterization of Class 2 OLD family nucleases supports a two-metal catalysis mechanism for cleavage. United States. https://doi.org/10.1093/nar/gkz703
Schiltz, Carl J., Lee, April, Partlow, Edward A., Hosford, Christopher J., and Chappie, Joshua S. Sat .
"Structural characterization of Class 2 OLD family nucleases supports a two-metal catalysis mechanism for cleavage". United States. https://doi.org/10.1093/nar/gkz703. https://www.osti.gov/servlets/purl/1566816.
@article{osti_1566816,
title = {Structural characterization of Class 2 OLD family nucleases supports a two-metal catalysis mechanism for cleavage},
author = {Schiltz, Carl J. and Lee, April and Partlow, Edward A. and Hosford, Christopher J. and Chappie, Joshua S.},
abstractNote = {Overcoming lysogenization defect (OLD) proteins constitute a family of uncharacterized nucleases present in bacteria, archaea, and some viruses. These enzymes contain an N-terminal ATPase domain and a C-terminal Toprim domain common amongst replication, recombination, and repair proteins. The in vivo activities of OLD proteins remain poorly understood and no definitive structural information exists. Here we identify and define two classes of OLD proteins based on differences in gene neighborhood and amino acid sequence conservation and present the crystal structures of the catalytic C-terminal regions from the Burkholderia pseudomallei and Xanthamonas campestris p.v. campestris Class 2 OLD proteins at 2.24 Å and 1.86 Å resolution respectively. The structures reveal a two-domain architecture containing a Toprim domain with altered architecture and a unique helical domain. Conserved side chains contributed by both domains coordinate two bound magnesium ions in the active site of B. pseudomallei OLD in a geometry that supports a two-metal catalysis mechanism for cleavage. The spatial organization of these domains additionally suggests a novel mode of DNA binding that is distinct from other Toprim containing proteins. Together, these findings define the fundamental structural properties of the OLD family catalytic core and the underlying mechanism controlling nuclease activity.},
doi = {10.1093/nar/gkz703},
journal = {Nucleic Acids Research},
number = 17,
volume = 47,
place = {United States},
year = {Sat Aug 10 00:00:00 EDT 2019},
month = {Sat Aug 10 00:00:00 EDT 2019}
}
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Works referenced in this record:
Evaluation of methods for trace‐element determination with emphasis on their usability in the clinical routine laboratory
journal, January 2007
- Bolann, B. J.; Rahil‐Khazen, R.; Henriksen, H.
- Scandinavian Journal of Clinical and Laboratory Investigation, Vol. 67, Issue 4
How Cations Can Assist DNase I in DNA Binding and Hydrolysis
journal, November 2010
- Guéroult, Marc; Picot, Daniel; Abi-Ghanem, Joséphine
- PLoS Computational Biology, Vol. 6, Issue 11
Bacteriophage Lambda; Abortive Infection of Bacteria Lysogenic for Phage P2
journal, July 1970
- Lindahl, G.; Sironi, G.; Bialy, H.
- Proceedings of the National Academy of Sciences, Vol. 66, Issue 3
Making and Breaking Nucleic Acids: Two-Mg2+-Ion Catalysis and Substrate Specificity
journal, April 2006
- Yang, Wei; Lee, Jae Young; Nowotny, Marcin
- Molecular Cell, Vol. 22, Issue 1
Structural, functional and evolutionary relationships between homing endonucleases and proteins from their host organisms
journal, March 2012
- Taylor, G. K.; Stoddard, B. L.
- Nucleic Acids Research, Vol. 40, Issue 12
KEGG: new perspectives on genomes, pathways, diseases and drugs
journal, November 2016
- Kanehisa, Minoru; Furumichi, Miho; Tanabe, Mao
- Nucleic Acids Research, Vol. 45, Issue D1
Dali server: conservation mapping in 3D
journal, May 2010
- Holm, Liisa; Rosenstr�m, P�ivi
- Nucleic Acids Research, Vol. 38, Issue suppl_2
High-resolution Crystal Structure of the Restriction–modification Controller Protein C.AhdI from Aeromonas hydrophila
journal, February 2005
- McGeehan, J. E.; Streeter, S. D.; Papapanagiotou, I.
- Journal of Molecular Biology, Vol. 346, Issue 3
The 3′ to 5′ Exonuclease Activity of Mre11 Facilitates Repair of DNA Double-Strand Breaks
journal, June 1998
- Paull, Tanya T.; Gellert, Martin
- Molecular Cell, Vol. 1, Issue 7
A TOPRIM domain in the crystal structure of the catalytic core of Escherichia coli primase confirms a structural link to DNA topoisomerases 1 1Edited by P. E. Wright
journal, July 2000
- Podobnik, Marjetka; McInerney, Peter; O’Donnell, Mike
- Journal of Molecular Biology, Vol. 300, Issue 2
The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme
journal, December 1983
- Guerrier-Takada, Cecilia; Gardiner, Katheleen; Marsh, Terry
- Cell, Vol. 35, Issue 3
Type II restriction endonucleases: structure and mechanism
journal, March 2005
- Pingoud, A.; Fuxreiter, M.; Pingoud, V.
- Cellular and Molecular Life Sciences, Vol. 62, Issue 6
Binding Mechanism of Metal⋅NTP Substrates and Stringent-Response Alarmones to Bacterial DnaG-Type Primases
journal, September 2012
- Rymer, Richard U.; Solorio, Francisco A.; Tehranchi, Ashley K.
- Structure, Vol. 20, Issue 9
The structural basis of differential DNA sequence recognition by restriction–modification controller proteins
journal, August 2012
- Ball, N. J.; McGeehan, J. E.; Streeter, S. D.
- Nucleic Acids Research, Vol. 40, Issue 20
Catalytic Metal Ions and Enzymatic Processing of DNA and RNA
journal, January 2015
- Palermo, Giulia; Cavalli, Andrea; Klein, Michael L.
- Accounts of Chemical Research, Vol. 48, Issue 2
Structure, Molecular Mechanisms, and Evolutionary Relationships in DNA Topoisomerases
journal, June 2004
- Corbett, Kevin D.; Berger, James M.
- Annual Review of Biophysics and Biomolecular Structure, Vol. 33, Issue 1
Topoisomerases and site-specific recombinases: similarities in structure and mechanism
journal, July 2010
- Yang, Wei
- Critical Reviews in Biochemistry and Molecular Biology, Vol. 45, Issue 6
Mutants of Escherichia coli unable to be lysogenized by the temperate bacteriophage P2
journal, February 1969
- Sironi, G.
- Virology, Vol. 37, Issue 2
DNA Structure-specific Nuclease Activities in the Saccharomyces cerevisiae Rad50·Mre11 Complex
journal, July 2001
- Trujillo, Kelly M.; Sung, Patrick
- Journal of Biological Chemistry, Vol. 276, Issue 38
Bacteriophage P2-λ interference: Inhibition of protein synthesis involves transfer RNA inactivation
journal, December 1974
- Bregegere, Francois
- Journal of Molecular Biology, Vol. 90, Issue 3
Catalytic Mechanisms of Restriction and Homing Endonucleases
journal, November 2002
- Galburt, Eric A.; Stoddard, Barry L.
- Biochemistry, Vol. 41, Issue 47
Refined structure of alkaline phosphatase from Escherichia coli at 2.8 Å resolution
journal, November 1985
- Sowadski, Janusz M.; Handschumacher, Mark D.; Krishna Murthy, H. M.
- Journal of Molecular Biology, Vol. 186, Issue 2
Genome Scanning for Conditionally Essential Genes in Salmonella enterica Serotype Typhimurium
journal, February 2012
- Khatiwara, Anita; Jiang, Tieshan; Sung, Sam-Sun
- Applied and Environmental Microbiology, Vol. 78, Issue 9
Capture of a third Mg2+ is essential for catalyzing DNA synthesis
journal, June 2016
- Gao, Y.; Yang, W.
- Science, Vol. 352, Issue 6291
A short history of SHELX
journal, December 2007
- Sheldrick, George M.
- Acta Crystallographica Section A Foundations of Crystallography, Vol. 64, Issue 1, p. 112-122
Nbs1 potentiates ATP-driven DNA unwinding and endonuclease cleavage by the Mre11/Rad50 complex
journal, May 1999
- Paull, T. T.; Gellert, M.
- Genes & Development, Vol. 13, Issue 10
In vitro splicing of the ribosomal RNA precursor of tetrahymena: Involvement of a guanosine nucleotide in the excision of the intervening sequence
journal, December 1981
- Cech, Thomas R.; Zaug, Arthur J.; Grabowski, Paula J.
- Cell, Vol. 27, Issue 3
The crystal structure of the ternary complex of staphylococcal nuclease, Ca2+ and the inhibitor pdTp, refined at 1.65 Å
journal, January 1989
- Loll, Patrick J.; Lattman, Eaton E.
- Proteins: Structure, Function, and Genetics, Vol. 5, Issue 3
Improvements to the APBS biomolecular solvation software suite: Improvements to the APBS Software Suite
journal, October 2017
- Jurrus, Elizabeth; Engel, Dave; Star, Keith
- Protein Science, Vol. 27, Issue 1
The 5S rRNA maturase, ribonuclease M5, is a Toprim domain family member
journal, July 2005
- Allemand, F.
- Nucleic Acids Research, Vol. 33, Issue 13
The old exonuclease of bacteriophage P2.
journal, February 1995
- Myung, H.; Calendar, R.
- Journal of Bacteriology, Vol. 177, Issue 3
Connected gene neighborhoods in prokaryotic genomes
journal, May 2002
- Rogozin, I. B.
- Nucleic Acids Research, Vol. 30, Issue 10
Structural analysis of the genetic switch that regulates the expression of restriction-modification genes
journal, July 2008
- McGeehan, John E.; Streeter, Simon D.; Thresh, Sarah -J.
- Nucleic Acids Research, Vol. 36, Issue 14
Ca 2+ -dependent activity of human DNase I and its hyperactive variants
journal, January 1999
- Pan, Clark Q.; Lazarus, Robert A.
- Protein Science, Vol. 8, Issue 9
Toprim--a conserved catalytic domain in type IA and II topoisomerases, DnaG-type primases, OLD family nucleases and RecR proteins
journal, September 1998
- Aravind, L.
- Nucleic Acids Research, Vol. 26, Issue 18
A general two-metal-ion mechanism for catalytic RNA.
journal, July 1993
- Steitz, T. A.; Steitz, J. A.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 14
Structure of the RNA Polymerase Domain of E. coli Primase
journal, March 2000
- Keck, J. L.
- Science, Vol. 287, Issue 5462
Effects of magnesium and related divalent metal ions in topoisomerase structure and function
journal, February 2009
- Sissi, C.; Palumbo, M.
- Nucleic Acids Research, Vol. 37, Issue 3
The CWB2 Cell Wall-Anchoring Module Is Revealed by the Crystal Structures of the Clostridium difficile Cell Wall Proteins Cwp8 and Cwp6
journal, March 2017
- Usenik, Aleksandra; Renko, Miha; Mihelič, Marko
- Structure, Vol. 25, Issue 3
Bacteriophage P2: Interaction with phage lambda and with recombination-deficient bacteria
journal, November 1971
- Sironi, Gianpiero; Bialy, Harvey; Lozeron, Homer A.
- Virology, Vol. 46, Issue 2
An equivalent metal ion in one- and two-metal-ion catalysis
journal, October 2008
- Yang, Wei
- Nature Structural & Molecular Biology, Vol. 15, Issue 11
ConSurf 2016: an improved methodology to estimate and visualize evolutionary conservation in macromolecules
journal, May 2016
- Ashkenazy, Haim; Abadi, Shiran; Martz, Eric
- Nucleic Acids Research, Vol. 44, Issue W1
IMG/M: integrated genome and metagenome comparative data analysis system
journal, October 2016
- Chen, I-Min A.; Markowitz, Victor M.; Chu, Ken
- Nucleic Acids Research, Vol. 45, Issue D1
Structure and Mechanism of Helicases and Nucleic Acid Translocases
journal, June 2007
- Singleton, Martin R.; Dillingham, Mark S.; Wigley, Dale B.
- Annual Review of Biochemistry, Vol. 76, Issue 1
Cocrystal structure of an editing complex of Klenow fragment with DNA.
journal, December 1988
- Freemont, P. S.; Friedman, J. M.; Beese, L. S.
- Proceedings of the National Academy of Sciences, Vol. 85, Issue 23
Phaser crystallographic software
journal, July 2007
- McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
- Journal of Applied Crystallography, Vol. 40, Issue 4
A novel and unified two-metal mechanism for DNA cleavage by type II and IA topoisomerases
journal, June 2010
- Schmidt, Bryan H.; Burgin, Alex B.; Deweese, Joseph E.
- Nature, Vol. 465, Issue 7298
Diversity of limestone bacteriophages infecting Dickeya solani isolated in the Czech Republic
journal, February 2021
- Petrzik, Karel; Vacek, Josef; Brázdová, Sára
- Archives of Virology, Vol. 166, Issue 4
Improvements to the APBS biomolecular solvation software suite
text, January 2017
- Jurrus, Elizabeth; Engel, Dave; Star, Keith
- arXiv
The crystal structure of the ternary complex of staphylococcal nuclease, Ca2+ and the inhibitor pdTp, refined at 1.65 Å
journal, January 1989
- Loll, Patrick J.; Lattman, Eaton E.
- Proteins: Structure, Function, and Genetics, Vol. 5, Issue 3
X-Ray sensitivity of Escherichia coli lysogenic for bacteriophage P2
journal, January 1979
- Ghisotti, Daniela; Zangrossi, Sandro; Sironi, Gianpiero
- MGG Molecular & General Genetics, Vol. 169, Issue 3
Type II restriction endonucleases: structure and mechanism
journal, March 2005
- Pingoud, A.; Fuxreiter, M.; Pingoud, V.
- Cellular and Molecular Life Sciences, Vol. 62, Issue 6
Refined structure of alkaline phosphatase from Escherichia coli at 2.8 Å resolution
journal, November 1985
- Sowadski, Janusz M.; Handschumacher, Mark D.; Krishna Murthy, H. M.
- Journal of Molecular Biology, Vol. 186, Issue 2
Bacteriophage P2: Interaction with phage lambda and with recombination-deficient bacteria
journal, November 1971
- Sironi, Gianpiero; Bialy, Harvey; Lozeron, Homer A.
- Virology, Vol. 46, Issue 2
In vitro splicing of the ribosomal RNA precursor of tetrahymena: Involvement of a guanosine nucleotide in the excision of the intervening sequence
journal, December 1981
- Cech, Thomas R.; Zaug, Arthur J.; Grabowski, Paula J.
- Cell, Vol. 27, Issue 3
The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme
journal, December 1983
- Guerrier-Takada, Cecilia; Gardiner, Katheleen; Marsh, Terry
- Cell, Vol. 35, Issue 3
High-resolution Crystal Structure of the Restriction–modification Controller Protein C.AhdI from Aeromonas hydrophila
journal, February 2005
- McGeehan, J. E.; Streeter, S. D.; Papapanagiotou, I.
- Journal of Molecular Biology, Vol. 346, Issue 3
Binding Mechanism of Metal⋅NTP Substrates and Stringent-Response Alarmones to Bacterial DnaG-Type Primases
journal, September 2012
- Rymer, Richard U.; Solorio, Francisco A.; Tehranchi, Ashley K.
- Structure, Vol. 20, Issue 9
The CWB2 Cell Wall-Anchoring Module Is Revealed by the Crystal Structures of the Clostridium difficile Cell Wall Proteins Cwp8 and Cwp6
journal, March 2017
- Usenik, Aleksandra; Renko, Miha; Mihelič, Marko
- Structure, Vol. 25, Issue 3
Nucleases: diversity of structure, function and mechanism
journal, September 2010
- Yang, Wei
- Quarterly Reviews of Biophysics, Vol. 44, Issue 1
Anomalous diffraction in crystallographic phase evaluation
journal, February 2014
- Hendrickson, Wayne A.
- Quarterly Reviews of Biophysics, Vol. 47, Issue 1
Catalytic Metal Ions and Enzymatic Processing of DNA and RNA
journal, January 2015
- Palermo, Giulia; Cavalli, Andrea; Klein, Michael L.
- Accounts of Chemical Research, Vol. 48, Issue 2
A novel and unified two-metal mechanism for DNA cleavage by type II and IA topoisomerases
journal, June 2010
- Schmidt, Bryan H.; Burgin, Alex B.; Deweese, Joseph E.
- Nature, Vol. 465, Issue 7298
Bacteriophage Lambda; Abortive Infection of Bacteria Lysogenic for Phage P2
journal, July 1970
- Lindahl, G.; Sironi, G.; Bialy, H.
- Proceedings of the National Academy of Sciences, Vol. 66, Issue 3
Cocrystal structure of an editing complex of Klenow fragment with DNA.
journal, December 1988
- Freemont, P. S.; Friedman, J. M.; Beese, L. S.
- Proceedings of the National Academy of Sciences, Vol. 85, Issue 23
A general two-metal-ion mechanism for catalytic RNA.
journal, July 1993
- Steitz, T. A.; Steitz, J. A.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 14
Magnesium Coordination Controls the Molecular Switch Function of DNA Mismatch Repair Protein MutS
journal, February 2010
- Lebbink, Joyce H. G.; Fish, Alexander; Reumer, Annet
- Journal of Biological Chemistry, Vol. 285, Issue 17
Evaluation of methods for trace‐element determination with emphasis on their usability in the clinical routine laboratory
journal, January 2007
- Bolann, B. J.; Rahil‐Khazen, R.; Henriksen, H.
- Scandinavian Journal of Clinical and Laboratory Investigation, Vol. 67, Issue 4
Toprim--a conserved catalytic domain in type IA and II topoisomerases, DnaG-type primases, OLD family nucleases and RecR proteins
journal, September 1998
- Aravind, L.
- Nucleic Acids Research, Vol. 26, Issue 18
Connected gene neighborhoods in prokaryotic genomes
journal, May 2002
- Rogozin, I. B.
- Nucleic Acids Research, Vol. 30, Issue 10
The 5S rRNA maturase, ribonuclease M5, is a Toprim domain family member
journal, July 2005
- Allemand, F.
- Nucleic Acids Research, Vol. 33, Issue 13
Structural analysis of the genetic switch that regulates the expression of restriction-modification genes
journal, July 2008
- McGeehan, John E.; Streeter, Simon D.; Thresh, Sarah -J.
- Nucleic Acids Research, Vol. 36, Issue 14
Dali server: conservation mapping in 3D
journal, May 2010
- Holm, Liisa; Rosenstr�m, P�ivi
- Nucleic Acids Research, Vol. 38, Issue suppl_2
Structural, functional and evolutionary relationships between homing endonucleases and proteins from their host organisms
journal, March 2012
- Taylor, G. K.; Stoddard, B. L.
- Nucleic Acids Research, Vol. 40, Issue 12
The structural basis of differential DNA sequence recognition by restriction–modification controller proteins
journal, August 2012
- Ball, N. J.; McGeehan, J. E.; Streeter, S. D.
- Nucleic Acids Research, Vol. 40, Issue 20
KEGG: new perspectives on genomes, pathways, diseases and drugs
journal, November 2016
- Kanehisa, Minoru; Furumichi, Miho; Tanabe, Mao
- Nucleic Acids Research, Vol. 45, Issue D1
ConSurf 2016: an improved methodology to estimate and visualize evolutionary conservation in macromolecules
journal, May 2016
- Ashkenazy, Haim; Abadi, Shiran; Martz, Eric
- Nucleic Acids Research, Vol. 44, Issue W1
IMG/M: integrated genome and metagenome comparative data analysis system
journal, October 2016
- Chen, I-Min A.; Markowitz, Victor M.; Chu, Ken
- Nucleic Acids Research, Vol. 45, Issue D1
Nbs1 potentiates ATP-driven DNA unwinding and endonuclease cleavage by the Mre11/Rad50 complex
journal, May 1999
- Paull, T. T.; Gellert, M.
- Genes & Development, Vol. 13, Issue 10
Integration, scaling, space-group assignment and post-refinement
journal, January 2010
- Kabsch, Wolfgang
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 133-144
Ca 2+ -dependent activity of human DNase I and its hyperactive variants
journal, January 1999
- Pan, Clark Q.; Lazarus, Robert A.
- Protein Science, Vol. 8, Issue 9
The old exonuclease of bacteriophage P2.
journal, February 1995
- Myung, H.; Calendar, R.
- Journal of Bacteriology, Vol. 177, Issue 3
Structure and Mechanism of Helicases and Nucleic Acid Translocases
journal, June 2007
- Singleton, Martin R.; Dillingham, Mark S.; Wigley, Dale B.
- Annual Review of Biochemistry, Vol. 76, Issue 1
Structure, Molecular Mechanisms, and Evolutionary Relationships in DNA Topoisomerases
journal, June 2004
- Corbett, Kevin D.; Berger, James M.
- Annual Review of Biophysics and Biomolecular Structure, Vol. 33, Issue 1
How Cations Can Assist DNase I in DNA Binding and Hydrolysis
journal, November 2010
- Guéroult, Marc; Picot, Daniel; Abi-Ghanem, Joséphine
- PLoS Computational Biology, Vol. 6, Issue 11
Topoisomerases and site-specific recombinases: similarities in structure and mechanism
journal, July 2010
- Yang, Wei
- Critical Reviews in Biochemistry and Molecular Biology, Vol. 45, Issue 6
Improvements to the APBS biomolecular solvation software suite
text, January 2017
- Jurrus, Elizabeth; Engel, Dave; Star, Keith
- arXiv
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