A Copper Relay System Involving Two Periplasmic Chaperones Drives cbb3-Type Cytochrome c Oxidase Biogenesis in Rhodobacter capsulatus
Abstract
PccA and SenC are periplasmic copper chaperones required for the biogenesis of cbb3-type cytochrome c oxidase (cbb3-Cox) in Rhodobacter capsulatus at physiological Cu concentrations. However, both proteins are dispensable for cbb3-Cox assembly when the external Cu concentration is high. PccA and SenC bind Cu using Met and His residues and Cys and His residues as ligands, respectively, and both proteins form a complex during cbb3-Cox biogenesis. SenC also interacts directly with cbb3-Cox, as shown by chemical cross-linking. Here we determined the periplasmic concentrations of both proteins in vivo and analyzed their Cu binding stoichiometries and their Cu(I) and Cu(II) binding affinity constants (KD) in vitro. Our data show that both proteins bind a single Cu atom with high affinity. In vitro Cu transfer assays demonstrate Cu transfer both from PccA to SenC and from SenC to PccA at similar levels. We conclude that PccA and SenC constitute a Cu relay system that facilitates Cu delivery to cbb3-Cox.
- Authors:
-
[2]
- Univ. of Pennsylvania, Philadelphia, PA (United States); Univ. of Freiburg (Germany)
- Univ. of Freiburg (Germany)
- Univ. of Pennsylvania, Philadelphia, PA (United States)
- Publication Date:
- Research Org.:
- Univ. of Pennsylvania, Philadelphia, PA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1546085
- Grant/Contract Number:
- FG02-91ER20052
- Resource Type:
- Accepted Manuscript
- Journal Name:
- ACS Chemical Biology
- Additional Journal Information:
- Journal Volume: 13; Journal Issue: 5; Journal ID: ISSN 1554-8929
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Trasnea, Petru-Iulian, Andrei, Andreea, Marckmann, Dorian, Utz, Marcel, Khalfaoui-Hassani, Bahia, Selamoglu, Nur, Daldal, Fevzi, and Koch, Hans-Georg. A Copper Relay System Involving Two Periplasmic Chaperones Drives cbb3-Type Cytochrome c Oxidase Biogenesis in Rhodobacter capsulatus. United States: N. p., 2018.
Web. doi:10.1021/acschembio.8b00293.
Trasnea, Petru-Iulian, Andrei, Andreea, Marckmann, Dorian, Utz, Marcel, Khalfaoui-Hassani, Bahia, Selamoglu, Nur, Daldal, Fevzi, & Koch, Hans-Georg. A Copper Relay System Involving Two Periplasmic Chaperones Drives cbb3-Type Cytochrome c Oxidase Biogenesis in Rhodobacter capsulatus. United States. https://doi.org/10.1021/acschembio.8b00293
Trasnea, Petru-Iulian, Andrei, Andreea, Marckmann, Dorian, Utz, Marcel, Khalfaoui-Hassani, Bahia, Selamoglu, Nur, Daldal, Fevzi, and Koch, Hans-Georg. Tue .
"A Copper Relay System Involving Two Periplasmic Chaperones Drives cbb3-Type Cytochrome c Oxidase Biogenesis in Rhodobacter capsulatus". United States. https://doi.org/10.1021/acschembio.8b00293. https://www.osti.gov/servlets/purl/1546085.
@article{osti_1546085,
title = {A Copper Relay System Involving Two Periplasmic Chaperones Drives cbb3-Type Cytochrome c Oxidase Biogenesis in Rhodobacter capsulatus},
author = {Trasnea, Petru-Iulian and Andrei, Andreea and Marckmann, Dorian and Utz, Marcel and Khalfaoui-Hassani, Bahia and Selamoglu, Nur and Daldal, Fevzi and Koch, Hans-Georg},
abstractNote = {PccA and SenC are periplasmic copper chaperones required for the biogenesis of cbb3-type cytochrome c oxidase (cbb3-Cox) in Rhodobacter capsulatus at physiological Cu concentrations. However, both proteins are dispensable for cbb3-Cox assembly when the external Cu concentration is high. PccA and SenC bind Cu using Met and His residues and Cys and His residues as ligands, respectively, and both proteins form a complex during cbb3-Cox biogenesis. SenC also interacts directly with cbb3-Cox, as shown by chemical cross-linking. Here we determined the periplasmic concentrations of both proteins in vivo and analyzed their Cu binding stoichiometries and their Cu(I) and Cu(II) binding affinity constants (KD) in vitro. Our data show that both proteins bind a single Cu atom with high affinity. In vitro Cu transfer assays demonstrate Cu transfer both from PccA to SenC and from SenC to PccA at similar levels. We conclude that PccA and SenC constitute a Cu relay system that facilitates Cu delivery to cbb3-Cox.},
doi = {10.1021/acschembio.8b00293},
journal = {ACS Chemical Biology},
number = 5,
volume = 13,
place = {United States},
year = {Tue Apr 03 00:00:00 EDT 2018},
month = {Tue Apr 03 00:00:00 EDT 2018}
}
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