Thermochemical wastewater valorization via enhanced microbial toxicity tolerance
Abstract
Thermochemical (TC) biomass conversion processes such as pyrolysis and liquefaction generate considerable amounts of wastewater, which often contains highly toxic compounds that are incredibly challenging to convert via standard wastewater treatment approaches such as anaerobic digestion. These streams represent a cost for TC biorefineries, and a potential valorization opportunity, if effective conversion methods are developed. The primary challenge hindering microbial conversion of TC wastewater is toxicity. In this study, we employ a robust bacterium, Pseudomonas putida, with TC wastewater streams to demonstrate that aldehydes are the most inhibitory compounds in these streams. Proteomics, transcriptomics, and fluorescence-based immunoassays of P. putida grown in a representative wastewater stream indicate that stress results from protein damage, which we hypothesize is a primary toxicity mechanism. Constitutive overexpression of the chaperone genes, groEL, groES, and clpB, in a genome-reduced P. putida strain improves the tolerance towards multiple TC wastewater samples up to 200-fold. Moreover, the concentration ranges of TC wastewater are industrially relevant for further bioprocess development for all wastewater streams examined here, representing different TC process configurations. Furthermore, we demonstrate proof-of-concept polyhydroxyalkanoate production from the usable carbon in an exemplary TC wastewater stream. Altogether, this study demonstrates that protein quality control machinery and repairmore »
- Authors:
-
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Iowa State Univ., Ames, IA (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); LanzaTech, Inc., Skokie, IL (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1542241
- Alternate Identifier(s):
- OSTI ID: 1434804
- Report Number(s):
- NREL/JA-2A00-71485
Journal ID: ISSN 1754-5692; EESNBY
- Grant/Contract Number:
- AC05-00OR22725; AC36-08GO28308; AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Energy & Environmental Science
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 6; Journal ID: ISSN 1754-5692
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS
Citation Formats
Jayakody, Lahiru N., Johnson, Christopher W., Whitham, Jason M., Giannone, Richard J., Black, Brenna A., Cleveland, Nicholas S., Klingeman, Dawn Marie, Michener, William E., Olstad, Jessica L., Vardon, Derek R., Brown, Robert C., Brown, Steven D., Hettich, Robert L., Guss, Adam M., and Beckham, Gregg T. Thermochemical wastewater valorization via enhanced microbial toxicity tolerance. United States: N. p., 2018.
Web. doi:10.1039/c8ee00460a.
Jayakody, Lahiru N., Johnson, Christopher W., Whitham, Jason M., Giannone, Richard J., Black, Brenna A., Cleveland, Nicholas S., Klingeman, Dawn Marie, Michener, William E., Olstad, Jessica L., Vardon, Derek R., Brown, Robert C., Brown, Steven D., Hettich, Robert L., Guss, Adam M., & Beckham, Gregg T. Thermochemical wastewater valorization via enhanced microbial toxicity tolerance. United States. https://doi.org/10.1039/c8ee00460a
Jayakody, Lahiru N., Johnson, Christopher W., Whitham, Jason M., Giannone, Richard J., Black, Brenna A., Cleveland, Nicholas S., Klingeman, Dawn Marie, Michener, William E., Olstad, Jessica L., Vardon, Derek R., Brown, Robert C., Brown, Steven D., Hettich, Robert L., Guss, Adam M., and Beckham, Gregg T. Tue .
"Thermochemical wastewater valorization via enhanced microbial toxicity tolerance". United States. https://doi.org/10.1039/c8ee00460a. https://www.osti.gov/servlets/purl/1542241.
@article{osti_1542241,
title = {Thermochemical wastewater valorization via enhanced microbial toxicity tolerance},
author = {Jayakody, Lahiru N. and Johnson, Christopher W. and Whitham, Jason M. and Giannone, Richard J. and Black, Brenna A. and Cleveland, Nicholas S. and Klingeman, Dawn Marie and Michener, William E. and Olstad, Jessica L. and Vardon, Derek R. and Brown, Robert C. and Brown, Steven D. and Hettich, Robert L. and Guss, Adam M. and Beckham, Gregg T.},
abstractNote = {Thermochemical (TC) biomass conversion processes such as pyrolysis and liquefaction generate considerable amounts of wastewater, which often contains highly toxic compounds that are incredibly challenging to convert via standard wastewater treatment approaches such as anaerobic digestion. These streams represent a cost for TC biorefineries, and a potential valorization opportunity, if effective conversion methods are developed. The primary challenge hindering microbial conversion of TC wastewater is toxicity. In this study, we employ a robust bacterium, Pseudomonas putida, with TC wastewater streams to demonstrate that aldehydes are the most inhibitory compounds in these streams. Proteomics, transcriptomics, and fluorescence-based immunoassays of P. putida grown in a representative wastewater stream indicate that stress results from protein damage, which we hypothesize is a primary toxicity mechanism. Constitutive overexpression of the chaperone genes, groEL, groES, and clpB, in a genome-reduced P. putida strain improves the tolerance towards multiple TC wastewater samples up to 200-fold. Moreover, the concentration ranges of TC wastewater are industrially relevant for further bioprocess development for all wastewater streams examined here, representing different TC process configurations. Furthermore, we demonstrate proof-of-concept polyhydroxyalkanoate production from the usable carbon in an exemplary TC wastewater stream. Altogether, this study demonstrates that protein quality control machinery and repair mechanisms can enable substantial gains in microbial tolerance to highly toxic substrates, including heterogeneous waste streams. When coupled to other metabolic engineering advances such as expanded substrate utilization and enhanced product accumulation, this study generally enables new strategies for biological conversion of highly-toxic, organic-rich wastewater via engineered aerobic monocultures or designer consortia.},
doi = {10.1039/c8ee00460a},
journal = {Energy & Environmental Science},
number = 6,
volume = 11,
place = {United States},
year = {Tue Apr 17 00:00:00 EDT 2018},
month = {Tue Apr 17 00:00:00 EDT 2018}
}
Web of Science
Figures / Tables:
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