Genetically Engineered Elastomeric Polymer Network through Protein Zipper Assembly

Fazelinia, Hossein; Balog, Eva Rose M.; Desireddy, Anil; Chakraborty, Saumen; Sheehan, Chris J.; Strauss, Charlie E. M.; Martinez, Jennifer S.

doi:10.1002/slct.201700456

Title: Genetically Engineered Elastomeric Polymer Network through Protein Zipper Assembly

Abstract

The rich palette of chemical and functional diversity found within peptides has driven the recent interest in bottom–up assembly of soft polypeptide–based materials. Herein, we describe the creation of a novel helix–elastin like polymer or HELP. HELP marries the specificity of coiled–coil interactions with the elasticity and stimuli–responsive behavior of elastin, a class of polymers with extraordinary elasticity. We used Rosetta to computationally design highly specific protein helical zippers to connect short elastin segments. As a result, the programmed pairing between the helical blocks of two individual chains produced a genetically encoded material that, when deposited on a substrate, formed a flexible and porous 2D planar network with controllable porosity that responds dynamically upon application of stimuli.

Authors:

Fazelinia, Hossein ^[1]; Balog, Eva Rose M. ^[1]; Desireddy, Anil ^[1]; Chakraborty, Saumen ^[1]; Sheehan, Chris J. ^[1];

^[1];

^[1]

Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

Publication Date:: Tue Jul 18 00:00:00 EDT 2017

Research Org.:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1498046

Alternate Identifier(s):: OSTI ID: 1373958

Report Number(s):: LA-UR-16-20868
Journal ID: ISSN 2365-6549

Grant/Contract Number:: 89233218CNA000001

Resource Type:: Accepted Manuscript

Journal Name:: Chemistry Select

Additional Journal Information:: Journal Volume: 2; Journal Issue: 18; Journal ID: ISSN 2365-6549

Publisher:: Wiley

Country of Publication:: United States

Language:: English

Subject:: 36 MATERIALS SCIENCE; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Material Science; Elastin like polymer; Helical zipper; Protein design; Self-assembly; Stimuli responsive polymer

Citation Formats


                    Fazelinia, Hossein, Balog, Eva Rose M., Desireddy, Anil, Chakraborty, Saumen, Sheehan, Chris J., Strauss, Charlie E. M., and Martinez, Jennifer S. Genetically Engineered Elastomeric Polymer Network through Protein Zipper Assembly.  United States: N. p., 2017. 
Web.  doi:10.1002/slct.201700456.

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                    Fazelinia, Hossein, Balog, Eva Rose M., Desireddy, Anil, Chakraborty, Saumen, Sheehan, Chris J., Strauss, Charlie E. M., & Martinez, Jennifer S. Genetically Engineered Elastomeric Polymer Network through Protein Zipper Assembly.  United States.  https://doi.org/10.1002/slct.201700456

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                    Fazelinia, Hossein, Balog, Eva Rose M., Desireddy, Anil, Chakraborty, Saumen, Sheehan, Chris J., Strauss, Charlie E. M., and Martinez, Jennifer S. Tue .  
"Genetically Engineered Elastomeric Polymer Network through Protein Zipper Assembly".  United States.  https://doi.org/10.1002/slct.201700456.  https://www.osti.gov/servlets/purl/1498046.

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@article{osti_1498046,

  title        = {Genetically Engineered Elastomeric Polymer Network through Protein Zipper Assembly},

  author       = {Fazelinia, Hossein and Balog, Eva Rose M. and Desireddy, Anil and Chakraborty, Saumen and Sheehan, Chris J. and Strauss, Charlie E. M. and Martinez, Jennifer S.},

  abstractNote = {The rich palette of chemical and functional diversity found within peptides has driven the recent interest in bottom–up assembly of soft polypeptide–based materials. Herein, we describe the creation of a novel helix–elastin like polymer or HELP. HELP marries the specificity of coiled–coil interactions with the elasticity and stimuli–responsive behavior of elastin, a class of polymers with extraordinary elasticity. We used Rosetta to computationally design highly specific protein helical zippers to connect short elastin segments. As a result, the programmed pairing between the helical blocks of two individual chains produced a genetically encoded material that, when deposited on a substrate, formed a flexible and porous 2D planar network with controllable porosity that responds dynamically upon application of stimuli.},

  doi          = {10.1002/slct.201700456},

  journal      = {Chemistry Select},

  number       = 18,

  volume       = 2,

  place        = {United States},

  year         = {Tue Jul 18 00:00:00 EDT 2017},

  month        = {Tue Jul 18 00:00:00 EDT 2017}

}

Copy to clipboard

Journal Article:

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Accepted Manuscript (DOE)

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https://doi.org/10.1002/slct.201700456

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Figures / Tables:

Figure 1: Temperature responsive 2D networks of an elastomeric polymer are created using helical zippers. Schematic showing the design principal of stimuli-responsive Helix- Elastin Like Polymers (HELP) that shrinks and expands as a function of change in temperature. Although Elastin Like Polymers [ELP: (VPGIG)₈] typically form insoluble coacervates with increasesmore »

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Figures / Tables found in this record:

Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.

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Title: Genetically Engineered Elastomeric Polymer Network through Protein Zipper Assembly

Abstract

Citation Formats

Figures / Tables:

Building fibrous biomaterials from α-helical and collagen-like coiled-coil peptides journal, January 2010

Squaring the Circle in Peptide Assembly: From Fibers to Discrete Nanostructures by de Novo Design journal, September 2012

Design and engineering of silk fibroin scaffolds with biomimetic hierarchical structures journal, January 2013

Biomimetic collagen/elastin meshes for ventral hernia repair in a rat model journal, March 2017

Calcium Binding Peptide Motifs from Calmodulin Confer Divalent Ion Selectivity to Elastin-Like Polypeptides journal, June 2013

Accurate design of co-assembling multi-component protein nanomaterials journal, May 2014

Tunable Self-Assembly of Genetically Engineered Silk–Elastin-like Protein Polymers journal, November 2011

Biomimetic spinning of artificial spider silk from a chimeric minispidroin journal, January 2017

Computational Design of Self-Assembling Protein Nanomaterials with Atomic Level Accuracy journal, May 2012

Structural specificity in coiled-coil interactions journal, August 2008

Biomimetic Nanofibrillation in Two-Component Biopolymer Blends with Structural Analogs to Spider Silk journal, October 2016

Novel Rolling Circle Amplification and DNA Origami-Based DNA Belt-Involved Signal Amplification Assay for Highly Sensitive Detection of Prostate-Specific Antigen (PSA) journal, October 2014

Design of ordered two-dimensional arrays mediated by noncovalent protein-protein interfaces journal, June 2015

Elastic protein-based polymers in soft tissue augmentation and generation journal, January 1998

New currency for old rope: from coiled-coil assemblies to α-helical barrels journal, August 2012

The Design of Coiled-Coil Structures and Assemblies book, January 2005

Predicting helix orientation for coiled-coil dimers journal, May 2008

Comparison between Coacervation Property and Secondary Structure of Synthetic Peptides, Ile-containing Elastin-derived Pentapeptide Repeats journal, June 2013

Complete Recombinant Silk-Elastinlike Protein-Based Tissue Scaffold journal, December 2010

Biomimetic organization of collagen matrices to template bone-like microstructures journal, May 2016

Design and application of stimulus-responsive peptide systems journal, January 2007

Modular Design in Natural and Biomimetic Soft Materials journal, September 2011

Biomimetic Magnetic Silk Scaffolds journal, March 2015

New materials from proteins and peptides journal, August 2012

Design of a single-chain polypeptide tetrahedron assembled from coiled-coil segments journal, April 2013

Elastin: a representative ideal protein elastomer journal, February 2002

Metal-directed, chemically tunable assembly of one-, two- and three-dimensional crystalline protein arrays journal, March 2012

Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries journal, February 2011

Directed patterning of the self-assembled silk-elastin-like nanofibers using a nanomechanical stimulus journal, January 2012

Structural requirements essential for elastin coacervation: favorable spatial arrangements of valine ridges on the three-dimensional structure of elastin-derived polypeptide (VPGVG)n journal, September 2011

Modeling structurally variable regions in homologous proteins with rosetta journal, April 2004

Computational Design of Virus-Like Protein Assemblies on Carbon Nanotube Surfaces journal, May 2011

Semirational design of Jun-Fos coiled coils with increased affinity: Universal implications for leucine zipper prediction and design journal, June 2006

Recombinant human elastin polypeptides self-assemble into biomaterials with elastin-like properties journal, November 2003

Switchable Elastin-Like Polypeptides that Respond to Chemical Inducers of Dimerization journal, March 2013

Collagen-Based Biomimetic Nanofibrous Scaffolds: Preparation and Characterization of Collagen/Silk Fibroin Bicomponent Nanofibrous Structures journal, April 2008

Self-Assembling Cages from Coiled-Coil Peptide Modules journal, April 2013

Protein Structure Prediction Using Rosetta book, January 2004

Computational design of a protein crystal journal, April 2012

Selectional and Mutational Scope of Peptides Sequestering the Jun–Fos Coiled-Coil Domain journal, August 2008

Photoresponsive Retinal-Modified Silk–Elastin Copolymer journal, February 2013

LEGO-like DNA Structures journal, November 2012

Building fibrous biomaterials from α-helical and collagen-like coiled-coil peptides
journal, January 2010

Squaring the Circle in Peptide Assembly: From Fibers to Discrete Nanostructures by de Novo Design
journal, September 2012

Design and engineering of silk fibroin scaffolds with biomimetic hierarchical structures
journal, January 2013

Biomimetic collagen/elastin meshes for ventral hernia repair in a rat model
journal, March 2017

Calcium Binding Peptide Motifs from Calmodulin Confer Divalent Ion Selectivity to Elastin-Like Polypeptides
journal, June 2013

Accurate design of co-assembling multi-component protein nanomaterials
journal, May 2014

Tunable Self-Assembly of Genetically Engineered Silk–Elastin-like Protein Polymers
journal, November 2011

Biomimetic spinning of artificial spider silk from a chimeric minispidroin
journal, January 2017

Computational Design of Self-Assembling Protein Nanomaterials with Atomic Level Accuracy
journal, May 2012

Structural specificity in coiled-coil interactions
journal, August 2008

Biomimetic Nanofibrillation in Two-Component Biopolymer Blends with Structural Analogs to Spider Silk
journal, October 2016

Novel Rolling Circle Amplification and DNA Origami-Based DNA Belt-Involved Signal Amplification Assay for Highly Sensitive Detection of Prostate-Specific Antigen (PSA)
journal, October 2014

Design of ordered two-dimensional arrays mediated by noncovalent protein-protein interfaces
journal, June 2015

Elastic protein-based polymers in soft tissue augmentation and generation
journal, January 1998

New currency for old rope: from coiled-coil assemblies to α-helical barrels
journal, August 2012

The Design of Coiled-Coil Structures and Assemblies
book, January 2005

Predicting helix orientation for coiled-coil dimers
journal, May 2008

Comparison between Coacervation Property and Secondary Structure of Synthetic Peptides, Ile-containing Elastin-derived Pentapeptide Repeats
journal, June 2013

Complete Recombinant Silk-Elastinlike Protein-Based Tissue Scaffold
journal, December 2010

Biomimetic organization of collagen matrices to template bone-like microstructures
journal, May 2016

Design and application of stimulus-responsive peptide systems
journal, January 2007

Modular Design in Natural and Biomimetic Soft Materials
journal, September 2011

Biomimetic Magnetic Silk Scaffolds
journal, March 2015

New materials from proteins and peptides
journal, August 2012

Design of a single-chain polypeptide tetrahedron assembled from coiled-coil segments
journal, April 2013

Elastin: a representative ideal protein elastomer
journal, February 2002

Metal-directed, chemically tunable assembly of one-, two- and three-dimensional crystalline protein arrays
journal, March 2012

Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries
journal, February 2011

Directed patterning of the self-assembled silk-elastin-like nanofibers using a nanomechanical stimulus
journal, January 2012

Structural requirements essential for elastin coacervation: favorable spatial arrangements of valine ridges on the three-dimensional structure of elastin-derived polypeptide (VPGVG)n
journal, September 2011

Modeling structurally variable regions in homologous proteins with rosetta
journal, April 2004

Computational Design of Virus-Like Protein Assemblies on Carbon Nanotube Surfaces
journal, May 2011

Semirational design of Jun-Fos coiled coils with increased affinity: Universal implications for leucine zipper prediction and design
journal, June 2006

Recombinant human elastin polypeptides self-assemble into biomaterials with elastin-like properties
journal, November 2003

Switchable Elastin-Like Polypeptides that Respond to Chemical Inducers of Dimerization
journal, March 2013

Collagen-Based Biomimetic Nanofibrous Scaffolds: Preparation and Characterization of Collagen/Silk Fibroin Bicomponent Nanofibrous Structures
journal, April 2008

Self-Assembling Cages from Coiled-Coil Peptide Modules
journal, April 2013

Protein Structure Prediction Using Rosetta
book, January 2004

Computational design of a protein crystal
journal, April 2012

Selectional and Mutational Scope of Peptides Sequestering the Jun–Fos Coiled-Coil Domain
journal, August 2008

Photoresponsive Retinal-Modified Silk–Elastin Copolymer
journal, February 2013

LEGO-like DNA Structures
journal, November 2012