Biomass-degrading enzymes are catabolite repressed in anaerobic gut fungi
Abstract
Anaerobic fungi are among the most active plant‐degrading microbes in nature. Increased insight into the mechanisms and environmental cues that regulate fungal hydrolysis would better inform bioprocessing strategies to depolymerize lignocellulose. Here, we compare the response of three strains of anaerobic fungi ( Piromyces finnis , Anaeromyces robustus , and Neocallimastix californiae ) to catabolite regulation by simple carbohydrates. Anaerobic fungi exhibited high enzymatic activity against crystalline cellulose, which was repressed upon incubation with free sugars. Cellulolytic degradation was also inhibited when fungi were exposed to sugars they did not metabolize, suggesting a general mode of catabolite repression. RNA‐Seq experiments in the presence of excess glucose confirmed repression of carbohydrate active enzymes during sugar uptake, and offer a path towards unmasking the function of co‐regulated genes that could be involved in biomass degradation. Overall, these results suggest that sugar‐rich hydrolysates tune the behavior of anaerobic fungi by dampening production of their biomass‐degrading enzymes. © 2018 American Institute of Chemical Engineers AIChE J , 64: 4263–4270, 2018
- Publication Date:
- Research Org.:
- Univ. of California, Santa Barbara, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
- OSTI Identifier:
- 1485151
- Alternate Identifier(s):
- OSTI ID: 1475228
- Grant/Contract Number:
- SC0010352; AC02-05CH11231; AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- AIChE Journal
- Additional Journal Information:
- Journal Volume: 64; Journal Issue: 12; Journal ID: ISSN 0001-1541
- Publisher:
- American Institute of Chemical Engineers
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; anaerobic fungi; cellulases; biofuel; regulation; Neocallimastigales
Citation Formats
Henske, John K., Gilmore, Sean P., Haitjema, Charles H., Solomon, Kevin V., and O'Malley, Michelle A. Biomass-degrading enzymes are catabolite repressed in anaerobic gut fungi. United States: N. p., 2018.
Web. doi:10.1002/aic.16395.
Henske, John K., Gilmore, Sean P., Haitjema, Charles H., Solomon, Kevin V., & O'Malley, Michelle A. Biomass-degrading enzymes are catabolite repressed in anaerobic gut fungi. United States. https://doi.org/10.1002/aic.16395
Henske, John K., Gilmore, Sean P., Haitjema, Charles H., Solomon, Kevin V., and O'Malley, Michelle A. Thu .
"Biomass-degrading enzymes are catabolite repressed in anaerobic gut fungi". United States. https://doi.org/10.1002/aic.16395. https://www.osti.gov/servlets/purl/1485151.
@article{osti_1485151,
title = {Biomass-degrading enzymes are catabolite repressed in anaerobic gut fungi},
author = {Henske, John K. and Gilmore, Sean P. and Haitjema, Charles H. and Solomon, Kevin V. and O'Malley, Michelle A.},
abstractNote = {Anaerobic fungi are among the most active plant‐degrading microbes in nature. Increased insight into the mechanisms and environmental cues that regulate fungal hydrolysis would better inform bioprocessing strategies to depolymerize lignocellulose. Here, we compare the response of three strains of anaerobic fungi ( Piromyces finnis , Anaeromyces robustus , and Neocallimastix californiae ) to catabolite regulation by simple carbohydrates. Anaerobic fungi exhibited high enzymatic activity against crystalline cellulose, which was repressed upon incubation with free sugars. Cellulolytic degradation was also inhibited when fungi were exposed to sugars they did not metabolize, suggesting a general mode of catabolite repression. RNA‐Seq experiments in the presence of excess glucose confirmed repression of carbohydrate active enzymes during sugar uptake, and offer a path towards unmasking the function of co‐regulated genes that could be involved in biomass degradation. Overall, these results suggest that sugar‐rich hydrolysates tune the behavior of anaerobic fungi by dampening production of their biomass‐degrading enzymes. © 2018 American Institute of Chemical Engineers AIChE J , 64: 4263–4270, 2018},
doi = {10.1002/aic.16395},
journal = {AIChE Journal},
number = 12,
volume = 64,
place = {United States},
year = {Thu Aug 30 00:00:00 EDT 2018},
month = {Thu Aug 30 00:00:00 EDT 2018}
}
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