Functional Role of MrpA in the MrpABCDEFG Na+/H+ Antiporter Complex from the Archaeon Methanosarcina acetivorans
Abstract
The multisubunit cation/proton antiporter 3 family, also called Mrp, is widely distributed in all three phylogenetic domains (Eukarya, Bacteria, and Archaea). Investigations have focused on Mrp complexes from the domain Bacteria to the exclusion of Archaea, with a consensus emerging that all seven subunits are required for Na+/H+ antiport activity. The MrpA subunit from the MrpABCDEFG Na+/H+ antiporter complex of the archaeon Methanosarcina acetivorans was produced in antiporter-deficient Escherichia coli strains EP432 and KNabc and biochemically characterized to determine the role of MrpA in the complex. Both strains containing MrpA grew in the presence of up to 500 mM NaCl and pH values up to 11.0 with no added NaCl. Everted vesicles from the strains containing MrpA were able to generate a NADH-dependent pH gradient (ΔpH), which was abated by the addition of monovalent cations. The apparent Km values for Na+ and Li+ were similar and ranged from 31 to 63 mM, whereas activity was too low to determine the apparent Km for K+. Optimum activity was obtained between pH 7.0 and 8.0. Homology molecular modeling identified two half-closed symmetry-related ion translocation channels that are linked, forming a continuous path from the cytoplasm to the periplasm, analogous to the NuoLmore »
- Authors:
-
- National Inst. of Cardiology, Mexico City (Mexico). Dept. of Biochemistry; Pennsylvania State Univ., University Park, PA (United States). Dept. of Biochemistry and Molecular Biology
- Univ. of Guanajuato (Mexico). Division of Health Sciences and Engineering and Dept. of Agroindustrial Engineering
- National Inst. of Cardiology, Mexico City (Mexico). Dept. of Biochemistry
- Pennsylvania State Univ., University Park, PA (United States). Dept. of Biochemistry and Molecular Biology
- Publication Date:
- Research Org.:
- Pennsylvania State Univ., University Park, PA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division; National Science Foundation (NSF); National Council for Science and Technology (CONACYT) (Mexico)
- OSTI Identifier:
- 1465111
- Grant/Contract Number:
- FG02-95ER20198; 0820734; 156969; 979/2016; 169/2016
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Bacteriology
- Additional Journal Information:
- Journal Volume: 199; Journal Issue: 2; Journal ID: ISSN 0021-9193
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Methanosarcina; methanogen; sodium/proton antiport
Citation Formats
Jasso-Chávez, Ricardo, Diaz-Perez, César, Rodríguez-Zavala, José S., and Ferry, James G. Functional Role of MrpA in the MrpABCDEFG Na+/H+ Antiporter Complex from the Archaeon Methanosarcina acetivorans. United States: N. p., 2016.
Web. doi:10.1128/JB.00662-16.
Jasso-Chávez, Ricardo, Diaz-Perez, César, Rodríguez-Zavala, José S., & Ferry, James G. Functional Role of MrpA in the MrpABCDEFG Na+/H+ Antiporter Complex from the Archaeon Methanosarcina acetivorans. United States. https://doi.org/10.1128/JB.00662-16
Jasso-Chávez, Ricardo, Diaz-Perez, César, Rodríguez-Zavala, José S., and Ferry, James G. Mon .
"Functional Role of MrpA in the MrpABCDEFG Na+/H+ Antiporter Complex from the Archaeon Methanosarcina acetivorans". United States. https://doi.org/10.1128/JB.00662-16. https://www.osti.gov/servlets/purl/1465111.
@article{osti_1465111,
title = {Functional Role of MrpA in the MrpABCDEFG Na+/H+ Antiporter Complex from the Archaeon Methanosarcina acetivorans},
author = {Jasso-Chávez, Ricardo and Diaz-Perez, César and Rodríguez-Zavala, José S. and Ferry, James G.},
abstractNote = {The multisubunit cation/proton antiporter 3 family, also called Mrp, is widely distributed in all three phylogenetic domains (Eukarya, Bacteria, and Archaea). Investigations have focused on Mrp complexes from the domain Bacteria to the exclusion of Archaea, with a consensus emerging that all seven subunits are required for Na+/H+ antiport activity. The MrpA subunit from the MrpABCDEFG Na+/H+ antiporter complex of the archaeon Methanosarcina acetivorans was produced in antiporter-deficient Escherichia coli strains EP432 and KNabc and biochemically characterized to determine the role of MrpA in the complex. Both strains containing MrpA grew in the presence of up to 500 mM NaCl and pH values up to 11.0 with no added NaCl. Everted vesicles from the strains containing MrpA were able to generate a NADH-dependent pH gradient (ΔpH), which was abated by the addition of monovalent cations. The apparent Km values for Na+ and Li+ were similar and ranged from 31 to 63 mM, whereas activity was too low to determine the apparent Km for K+. Optimum activity was obtained between pH 7.0 and 8.0. Homology molecular modeling identified two half-closed symmetry-related ion translocation channels that are linked, forming a continuous path from the cytoplasm to the periplasm, analogous to the NuoL subunit of complex I. Bioinformatics analyses revealed genes encoding homologs of MrpABCDEFG in metabolically diverse methane-producing species. In conclusion, the results advance the biochemical, evolutionary, and physiological understanding of Mrp complexes that extends to the domain Archaea.},
doi = {10.1128/JB.00662-16},
journal = {Journal of Bacteriology},
number = 2,
volume = 199,
place = {United States},
year = {Mon Oct 31 00:00:00 EDT 2016},
month = {Mon Oct 31 00:00:00 EDT 2016}
}
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Works referencing / citing this record:
Mrp Antiporters Have Important Roles in Diverse Bacteria and Archaea
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