The Kinetochore Receptor for the Cohesin Loading Complex
Abstract
The ring-shaped cohesin complex brings together distant DNA domains to maintain, express, and segregate the genome. Establishing specific chromosomal linkages depends on cohesin recruitment to defined loci. One such locus is the budding yeast centromere, which is a paradigm for targeted cohesin loading. The kinetochore, a multiprotein complex that connects centromeres to microtubules, drives the recruitment of high levels of cohesin to link sister chromatids together. We have exploited this system to determine the mechanism of specific cohesin recruitment. We show that phosphorylation of the Ctf19 kinetochore protein by a conserved kinase, DDK, provides a binding site for the Scc2/4 cohesin loading complex, thereby directing cohesin loading to centromeres. A similar mechanism targets cohesin to chromosomes in vertebrates. These findings represent a complete molecular description of targeted cohesin loading, a phenomenon with wide-ranging importance in chromosome segregation and, in multicellular organisms, transcription regulation.
- Authors:
-
- Harvard Medical School, Boston, MA (United States)
- Univ. of Edinburgh (United Kingdom)
- Harvard Medical School, Boston, MA (United States); Howard Hughes Medical Inst., Chevy Chase, MD (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- Wellcome Centre for Cell Biology; National Institutes of Health (NIH)
- OSTI Identifier:
- 1430320
- Grant/Contract Number:
- 203149; P41 GM 103403
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Cell
- Additional Journal Information:
- Journal Volume: 171; Journal Issue: 1; Journal ID: ISSN 0092-8674
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; centromere; cohesin; kinetochore; cell cycle
Citation Formats
Hinshaw, Stephen M., Makrantoni, Vasso, Harrison, Stephen C., and Marston, Adèle L. The Kinetochore Receptor for the Cohesin Loading Complex. United States: N. p., 2017.
Web. doi:10.1016/j.cell.2017.08.017.
Hinshaw, Stephen M., Makrantoni, Vasso, Harrison, Stephen C., & Marston, Adèle L. The Kinetochore Receptor for the Cohesin Loading Complex. United States. https://doi.org/10.1016/j.cell.2017.08.017
Hinshaw, Stephen M., Makrantoni, Vasso, Harrison, Stephen C., and Marston, Adèle L. Thu .
"The Kinetochore Receptor for the Cohesin Loading Complex". United States. https://doi.org/10.1016/j.cell.2017.08.017. https://www.osti.gov/servlets/purl/1430320.
@article{osti_1430320,
title = {The Kinetochore Receptor for the Cohesin Loading Complex},
author = {Hinshaw, Stephen M. and Makrantoni, Vasso and Harrison, Stephen C. and Marston, Adèle L.},
abstractNote = {The ring-shaped cohesin complex brings together distant DNA domains to maintain, express, and segregate the genome. Establishing specific chromosomal linkages depends on cohesin recruitment to defined loci. One such locus is the budding yeast centromere, which is a paradigm for targeted cohesin loading. The kinetochore, a multiprotein complex that connects centromeres to microtubules, drives the recruitment of high levels of cohesin to link sister chromatids together. We have exploited this system to determine the mechanism of specific cohesin recruitment. We show that phosphorylation of the Ctf19 kinetochore protein by a conserved kinase, DDK, provides a binding site for the Scc2/4 cohesin loading complex, thereby directing cohesin loading to centromeres. A similar mechanism targets cohesin to chromosomes in vertebrates. These findings represent a complete molecular description of targeted cohesin loading, a phenomenon with wide-ranging importance in chromosome segregation and, in multicellular organisms, transcription regulation.},
doi = {10.1016/j.cell.2017.08.017},
journal = {Cell},
number = 1,
volume = 171,
place = {United States},
year = {Thu Sep 21 00:00:00 EDT 2017},
month = {Thu Sep 21 00:00:00 EDT 2017}
}
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