hLARP7 C-terminal domain contains an xRRM that binds the 3' hairpin of 7SK RNA
Abstract
The 7SK small nuclear ribonucleoprotein (snRNP) sequesters and inactivates the positive transcription elongation factor b (P-TEFb), an essential eukaryotic mRNA transcription factor. The human La-related protein group 7 (hLARP7) is a constitutive component of the 7SK snRNP and localizes to the 3' terminus of the 7SK long noncoding RNA. hLARP7, and in particular its C-terminal domain (CTD), is essential for 7SK RNA stability and assembly with P-TEFb. The hLARP7 N-terminal Lamodule binds and protects the 3' end from degradation, but the structural and functional role of its CTD is unclear.We report the solution NMR structure of the hLARP7 CTD and show that this domain contains an xRRM, a class of atypical RRM first identified in the Tetrahymena thermophila telomerase LARP7 protein p65. The xRRM binds the 3' end of 7SK RNA at the top of stem-loop 4 (SL4) and interacts with both unpaired and base-paired nucleotides. This study thus confirms that the xRRM is general to the LARP7 family of proteins and defines the binding site for hLARP7 on the 7SK RNA, providing insight into function.
- Authors:
-
- Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry
- Publication Date:
- Research Org.:
- Univ. of California, Los Angeles, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; National Inst. for Health (NIH); American Heart Association (AHA); American Cancer Society (ACS)
- OSTI Identifier:
- 1427800
- Grant/Contract Number:
- FC03-02ER63421; GM107567; T32 CA009056; 14POST20380737; 126777-PF-14-179- 01-DMC
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nucleic Acids Research
- Additional Journal Information:
- Journal Volume: 44; Journal Issue: 20; Journal ID: ISSN 0305-1048
- Publisher:
- Oxford University Press
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE
Citation Formats
Eichhorn, Catherine D., Chug, Rahul, and Feigon, Juli. hLARP7 C-terminal domain contains an xRRM that binds the 3' hairpin of 7SK RNA. United States: N. p., 2016.
Web. doi:10.1093/nar/gkw833.
Eichhorn, Catherine D., Chug, Rahul, & Feigon, Juli. hLARP7 C-terminal domain contains an xRRM that binds the 3' hairpin of 7SK RNA. United States. https://doi.org/10.1093/nar/gkw833
Eichhorn, Catherine D., Chug, Rahul, and Feigon, Juli. Mon .
"hLARP7 C-terminal domain contains an xRRM that binds the 3' hairpin of 7SK RNA". United States. https://doi.org/10.1093/nar/gkw833. https://www.osti.gov/servlets/purl/1427800.
@article{osti_1427800,
title = {hLARP7 C-terminal domain contains an xRRM that binds the 3' hairpin of 7SK RNA},
author = {Eichhorn, Catherine D. and Chug, Rahul and Feigon, Juli},
abstractNote = {The 7SK small nuclear ribonucleoprotein (snRNP) sequesters and inactivates the positive transcription elongation factor b (P-TEFb), an essential eukaryotic mRNA transcription factor. The human La-related protein group 7 (hLARP7) is a constitutive component of the 7SK snRNP and localizes to the 3' terminus of the 7SK long noncoding RNA. hLARP7, and in particular its C-terminal domain (CTD), is essential for 7SK RNA stability and assembly with P-TEFb. The hLARP7 N-terminal Lamodule binds and protects the 3' end from degradation, but the structural and functional role of its CTD is unclear.We report the solution NMR structure of the hLARP7 CTD and show that this domain contains an xRRM, a class of atypical RRM first identified in the Tetrahymena thermophila telomerase LARP7 protein p65. The xRRM binds the 3' end of 7SK RNA at the top of stem-loop 4 (SL4) and interacts with both unpaired and base-paired nucleotides. This study thus confirms that the xRRM is general to the LARP7 family of proteins and defines the binding site for hLARP7 on the 7SK RNA, providing insight into function.},
doi = {10.1093/nar/gkw833},
journal = {Nucleic Acids Research},
number = 20,
volume = 44,
place = {United States},
year = {Mon Sep 26 00:00:00 EDT 2016},
month = {Mon Sep 26 00:00:00 EDT 2016}
}
Web of Science
Works referenced in this record:
Gene expression in early stage cervical cancer
journal, March 2008
- Biewenga, Petra; Buist, Marrije R.; Moerland, Perry D.
- Gynecologic Oncology, Vol. 108, Issue 3
LARP7 is a potential tumor suppressor gene in gastric cancer
journal, April 2012
- Cheng, Yulan; Jin, Zhe; Agarwal, Rachana
- Laboratory Investigation, Vol. 92, Issue 7
Loss of function mutation in LARP7, chaperone of 7SK ncRNA, causes a syndrome of facial dysmorphism, intellectual disability, and primordial dwarfism
journal, August 2012
- Alazami, Anas M.; Al-Owain, Mohammad; Alzahrani, Fatema
- Human Mutation, Vol. 33, Issue 10
A novel occluded RNA recognition motif in Prp24 unwinds the U6 RNA internal stem loop
journal, June 2011
- Martin-Tumasz, Stephen; Richie, Ashley C.; Clos, Lawrence J.
- Nucleic Acids Research, Vol. 39, Issue 17
LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated
journal, February 2008
- Krueger, B. J.; Jeronimo, C.; Roy, B. B.
- Nucleic Acids Research, Vol. 36, Issue 7
RNA recognition motifs: boring? Not quite
journal, June 2008
- Cléry, Antoine; Blatter, Markus; Allain, Frédéric H-T
- Current Opinion in Structural Biology, Vol. 18, Issue 3
Isolated pseudo-RNA-recognition motifs of SR proteins can regulate splicing using a noncanonical mode of RNA recognition
journal, July 2013
- Clery, A.; Sinha, R.; Anczukow, O.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 30
Protein and RNA Dynamics Play Key Roles in Determining the Specific Recognition of GU-rich Polyadenylation Regulatory Elements by Human Cstf-64 Protein
journal, April 2005
- Deka, Pritilekha; Rajan, P. K.; Perez-Canadillas, Jose Manuel
- Journal of Molecular Biology, Vol. 347, Issue 4
Evolution of 7SK RNA and Its Protein Partners in Metazoa
journal, September 2009
- Marz, M.; Donath, A.; Verstraete, N.
- Molecular Biology and Evolution, Vol. 26, Issue 12
Invertebrate 7SK snRNAs
journal, January 2008
- Gruber, Andreas R.; Koper-Emde, Dorota; Marz, Manja
- Journal of Molecular Evolution, Vol. 66, Issue 2
RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP
journal, March 2013
- Muniz, L.; Egloff, S.; Kiss, T.
- Nucleic Acids Research, Vol. 41, Issue 8
Specificity of Hexim1 and Hexim2 Complex Formation with Cyclin T1/T2, Importin α and 7SK snRNA
journal, January 2010
- Czudnochowski, Nadine; Vollmuth, Friederike; Baumann, Sascha
- Journal of Molecular Biology, Vol. 395, Issue 1
KAP1 Recruitment of the 7SK snRNP Complex to Promoters Enables Transcription Elongation by RNA Polymerase II
text, January 2016
- P., McNamara, Ryan; L., McCann, Jennifer; E., Reeder, Jonathan
- The University of North Carolina at Chapel Hill University Libraries
A La-Related Protein Modulates 7SK snRNP Integrity to Suppress P-TEFb-Dependent Transcriptional Elongation and Tumorigenesis
journal, March 2008
- He, Nanhai; Jahchan, Nadine S.; Hong, Eunmee
- Molecular Cell, Vol. 29, Issue 5
Structural analyses of the 7SK ribonucleoprotein (RNP), the most abundant human small RNP of unknown function.
journal, July 1991
- Wassarman, D. A.; Steitz, J. A.
- Molecular and Cellular Biology, Vol. 11, Issue 7
HMGA1 directly interacts with TAR to modulate basal and Tat-dependent HIV transcription
journal, March 2013
- Eilebrecht, Sebastian; Wilhelm, Emmanuelle; Benecke, Bernd-Joachim
- RNA Biology, Vol. 10, Issue 3
U2AF homology motifs: protein recognition in the RRM world
journal, July 2004
- Kielkopf, C. L.
- Genes & Development, Vol. 18, Issue 13
NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition
journal, July 2006
- Dominguez, C.
- Nucleic Acids Research, Vol. 34, Issue 13
Transcription inhibition by flavopiridol: mechanism of chronic lymphocytic leukemia cell death
journal, October 2005
- Chen, Rong; Keating, Michael J.; Gandhi, Varsha
- Blood, Vol. 106, Issue 7
HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters
journal, March 2014
- Eilebrecht, Sebastian; Le Douce, Valentin; Riclet, Raphael
- Nucleic Acids Research, Vol. 42, Issue 8
7SK snRNA-mediated, gene-specific cooperativity of HMGA1 and P-TEFb
journal, November 2011
- Eilebrecht, Sebastian; Benecke, Bernd-Joachim; Benecke, Arndt
- RNA Biology, Vol. 8, Issue 6
Controlling Cellular P-TEFb Activity by the HIV-1 Transcriptional Transactivator Tat
journal, October 2010
- Muniz, Lisa; Egloff, Sylvain; Ughy, Bettina
- PLoS Pathogens, Vol. 6, Issue 10
Analysis of the interaction with the hepatitis C virus mRNA reveals an alternative mode of RNA recognition by the human La protein
journal, October 2011
- Martino, Luigi; Pennell, Simon; Kelly, Geoff
- Nucleic Acids Research, Vol. 40, Issue 3
xRRM: A new class of RRM found in the telomerase La family protein p65
journal, March 2013
- Singh, Mahavir; Choi, Charles P.; Feigon, Juli
- RNA Biology, Vol. 10, Issue 3
KAP1 Recruitment of the 7SK snRNP Complex to Promoters Enables Transcription Elongation by RNA Polymerase II
journal, January 2016
- McNamara, Ryan P.; Reeder, Jonathan E.; McMillan, Elizabeth A.
- Molecular Cell, Vol. 61, Issue 1
7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development
journal, April 2009
- Barboric, M.; Lenasi, T.; Chen, H.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 19
A La protein requirement for efficient pre-tRNA folding
journal, December 2003
- Chakshusmathi, G.
- The EMBO Journal, Vol. 22, Issue 24
A Rare Example of Lock-and-Key RNA Recognition
journal, December 2010
- Dethoff, Elizabeth A.; Al-Hashimi, Hashim M.
- Journal of Molecular Biology, Vol. 404, Issue 4
Recognition of GU-rich polyadenylation regulatory elements by human CstF-64 protein
journal, June 2003
- Perez Canadillas, J. M.
- The EMBO Journal, Vol. 22, Issue 11
Identification of protein coding regions by database similarity search
journal, March 1993
- Gish, Warren; States, David J.
- Nature Genetics, Vol. 3, Issue 3
Intracellular distribution of low molecular weight RNA species in HeLa cells
journal, November 1980
- Gurney, T.
- The Journal of Cell Biology, Vol. 87, Issue 2
Structural insights into the Cyclin T1–Tat–TAR RNA transcription activation complex from EIAV
journal, November 2008
- Anand, Kanchan; Schulte, Antje; Vogel-Bachmayr, Karin
- Nature Structural & Molecular Biology, Vol. 15, Issue 12
A comprehensive analysis of the La-motif protein superfamily
journal, March 2009
- Bousquet-Antonelli, C.; Deragon, J. -M.
- RNA, Vol. 15, Issue 5
The La-related protein LARP7 is a component of the 7SK ribonucleoprotein and affects transcription of cellular and viral polymerase II genes
journal, May 2008
- Markert, Andreas; Grimm, Michael; Martinez, Javier
- EMBO reports, Vol. 9, Issue 6
RNA helicase DDX21 coordinates transcription and ribosomal RNA processing
journal, November 2014
- Calo, Eliezer; Flynn, Ryan A.; Martin, Lance
- Nature, Vol. 518, Issue 7538
Stepwise protein-mediated RNA folding directs assembly of telomerase ribonucleoprotein
journal, February 2007
- Stone, Michael D.; Mihalusova, Mariana; O’Connor, Catherine M.
- Nature, Vol. 446, Issue 7134
5′ Processing of tRNA Precursors Can Be Modulated by the Human La Antigen Phosphoprotein
journal, June 1998
- Fan, Hao; Goodier, John L.; Chamberlain, Joel R.
- Molecular and Cellular Biology, Vol. 18, Issue 6
Distinct Conformational Transition Patterns of Noncoding 7SK snRNA and HIV TAR RNAs upon Tat Binding
journal, January 2014
- Lu, Jia; Wong, Vivian; Zhang, Yi
- Biochemistry, Vol. 53, Issue 4
A capping-independent function of MePCE in stabilizing 7SK snRNA and facilitating the assembly of 7SK snRNP
journal, November 2009
- Xue, Y.; Yang, Z.; Chen, R.
- Nucleic Acids Research, Vol. 38, Issue 2
The crystal structure of the Split End protein SHARP adds a new layer of complexity to proteins containing RNA recognition motifs
journal, April 2014
- Arieti, Fabiana; Gabus, Caroline; Tambalo, Margherita
- Nucleic Acids Research, Vol. 42, Issue 10
HIV-1 Tat Assembles a Multifunctional Transcription Elongation Complex and Stably Associates with the 7SK snRNP
journal, May 2010
- Sobhian, Bijan; Laguette, Nadine; Yatim, Ahmad
- Molecular Cell, Vol. 38, Issue 3
HMGA1-dependent and independent 7SK RNA gene regulatory activity
journal, January 2011
- Eilebrecht, Sebastian; Bécavin, Christophe; Léger, Hélène
- RNA Biology, Vol. 8, Issue 1
Structural insight into RNA recognition motifs: versatile molecular Lego building blocks for biological systems: Structural insight into RNA recognition motifs
journal, January 2012
- Muto, Yutaka; Yokoyama, Shigeyuki
- Wiley Interdisciplinary Reviews: RNA, Vol. 3, Issue 2
Controlling the Elongation Phase of Transcription with P-TEFb
journal, August 2006
- Peterlin, B. Matija; Price, David H.
- Molecular Cell, Vol. 23, Issue 3
The rrm Domain in Gw182 Proteins Contributes to Mirna-Mediated gene Silencing
dataset, January 2009
- Eulalio, A.; Tritschler, F.; Buettner, R.
- Biological Magnetic Resonance Bank
HEXIM1 targets a repeated GAUC motif in the riboregulator of transcription 7SK and promotes base pair rearrangements
journal, July 2010
- Lebars, Isabelle; Martinez-Zapien, D.; Durand, A.
- Nucleic Acids Research, Vol. 38, Issue 21
Structural basis of G-tract recognition and encaging by hnRNP F quasi-RRMs
journal, June 2010
- Dominguez, Cyril; Fisette, Jean-François; Chabot, Benoit
- Nature Structural & Molecular Biology, Vol. 17, Issue 7
The RRM domain in GW182 proteins contributes to miRNA-mediated gene silencing
journal, March 2009
- Eulalio, Ana; Tritschler, Felix; Büttner, Regina
- Nucleic Acids Research, Vol. 37, Issue 9
CDK9/CYCLIN T1 expression during normal lymphoid differentiation and malignant transformation
journal, January 2004
- Bellan, Cristiana; De Falco, Giulia; Lazzi, Stefano
- The Journal of Pathology, Vol. 203, Issue 4
Preformed Protein-binding Motifs in 7SK snRNA: Structural and Thermodynamic Comparisons with Retroviral TAR
journal, December 2010
- Durney, Michael A.; D'Souza, Victoria M.
- Journal of Molecular Biology, Vol. 404, Issue 4
Identification of protein coding regions by database similarity search
journal, March 1993
- Gish, Warren; States, David J.
- Nature Genetics, Vol. 3, Issue 3
RNA-based affinity purification reveals 7SK RNPs with distinct composition and regulation
journal, April 2007
- Hogg, J. R.; Collins, K.
- RNA, Vol. 13, Issue 6
Aberrant Nuclear Trafficking of La Protein Leads to Disordered Processing of Associated Precursor tRNAs
journal, May 2002
- Intine, Robert V.; Dundr, Miroslav; Misteli, Tom
- Molecular Cell, Vol. 9, Issue 5
Sjögren Syndrome Antigen B (SSB)/La Promotes Global MicroRNA Expression by Binding MicroRNA Precursors through Stem-Loop Recognition
journal, November 2012
- Liang, Chunyang; Xiong, Ke; Szulwach, Keith E.
- Journal of Biological Chemistry, Vol. 288, Issue 1
Hexim1 sequesters positive transcription elongation factor b from the class II transactivator on MHC class II promoters
journal, November 2006
- Kohoutek, J.; Blazek, D.; Peterlin, B. M.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 46
NMRPipe: A multidimensional spectral processing system based on UNIX pipes
journal, November 1995
- Delaglio, Frank; Grzesiek, Stephan; Vuister, GeertenW.
- Journal of Biomolecular NMR, Vol. 6, Issue 3
Compound heterozygous variants in the LARP7 gene as a cause of Alazami syndrome in a Caucasian female with significant failure to thrive, short stature, and developmental disability
journal, September 2015
- Ling, Tina T.; Sorrentino, Susanna
- American Journal of Medical Genetics Part A, Vol. 170, Issue 1
A mutation in T7 RNA polymerase that facilitates promoter clearance
journal, April 2005
- Guillerez, J.; Lopez, P. J.; Proux, F.
- Proceedings of the National Academy of Sciences, Vol. 102, Issue 17
SOFAST-HMQC Experiments for Recording Two-dimensional Deteronuclear Correlation Spectra of Proteins within a Few Seconds
journal, December 2005
- Schanda, Paul; Kupče, Ēriks; Brutscher, Bernhard
- Journal of Biomolecular NMR, Vol. 33, Issue 4
Measurement ofJand Dipolar Couplings from Simplified Two-Dimensional NMR Spectra
journal, April 1998
- Ottiger, Marcel; Delaglio, Frank; Bax, Ad
- Journal of Magnetic Resonance, Vol. 131, Issue 2
Evolution of 7SK RNA and Its Protein Partners in Metazoa
journal, September 2009
- Marz, M.; Donath, A.; Verstraete, N.
- Molecular Biology and Evolution, Vol. 26, Issue 12
The role of the C-terminal helix of U1A protein in the interaction with U1hpII RNA
journal, May 2013
- Law, M. J.; Lee, D. S.; Lee, C. S.
- Nucleic Acids Research, Vol. 41, Issue 14
Taking MLL through the MudPIT: Identification of Novel Complexes that Bring Together MLL-Fusion Proteins and Transcription Elongation Factors
journal, February 2010
- Fromm, George; Adelman, Karen
- Molecular Cell, Vol. 37, Issue 4
Structural Study of a DNA.cntdot.RNA Hybrid Duplex with a Chiral Phosphorothioate Moiety by NMR: Extraction of Distance and Torsion Angle Constraints and Imino Proton Exchange Rates
journal, September 1994
- Gonzalez, Carlos; Stec, Wojciech; Kobylanska, Anna
- Biochemistry, Vol. 33, Issue 37
7SK small nuclear RNA directly affects HMGA1 function in transcription regulation
journal, November 2010
- Eilebrecht, S.; Brysbaert, G.; Wegert, T.
- Nucleic Acids Research, Vol. 39, Issue 6
Loss of function mutation in LARP7, chaperone of 7SK ncRNA, causes a syndrome of facial dysmorphism, intellectual disability, and primordial dwarfism
journal, August 2012
- Alazami, Anas M.; Al-Owain, Mohammad; Alzahrani, Fatema
- Human Mutation, Vol. 33, Issue 10
AFF1 is a ubiquitous P-TEFb partner to enable Tat extraction of P-TEFb from 7SK snRNP and formation of SECs for HIV transactivation
journal, December 2013
- Lu, H.; Li, Z.; Xue, Y.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 1
NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: A novel mode of RNA recognition
text, January 2006
- Cyril, Dominguez,; H. -T., Allain, Frédéric
- ETH Zurich
U2AF homology motifs: protein recognition in the RRM world
journal, July 2004
- Kielkopf, C. L.
- Genes & Development, Vol. 18, Issue 13
Kick-sTARting HIV-1 transcription elongation by 7SK snRNP deporTATion
journal, August 2010
- Barboric, Matjaz; Lenasi, Tina
- Nature Structural & Molecular Biology, Vol. 17, Issue 8
AFF4, a Component of the ELL/P-TEFb Elongation Complex and a Shared Subunit of MLL Chimeras, Can Link Transcription Elongation to Leukemia
journal, February 2010
- Lin, Chengqi; Smith, Edwin R.; Takahashi, Hidehisa
- Molecular Cell, Vol. 37, Issue 3
RNA helicase DDX21 coordinates transcription and ribosomal RNA processing
journal, November 2014
- Calo, Eliezer; Flynn, Ryan A.; Martin, Lance
- Nature, Vol. 518, Issue 7538
Gene expression in early stage cervical cancer
journal, March 2008
- Biewenga, Petra; Buist, Marrije R.; Moerland, Perry D.
- Gynecologic Oncology, Vol. 108, Issue 3
Evidence for a base triple in the free HIV-1 TAR RNA
journal, March 2004
- Huthoff, H.
- RNA, Vol. 10, Issue 3
Structure of the C-Terminal Domain of Human La Protein Reveals a Novel RNA Recognition Motif Coupled to a Helical Nuclear Retention Element
journal, July 2003
- Jacks, Amanda; Babon, Jeff; Kelly, Geoff
- Structure, Vol. 11, Issue 7
RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP
journal, March 2013
- Muniz, L.; Egloff, S.; Kiss, T.
- Nucleic Acids Research, Vol. 41, Issue 8
Isolated pseudo-RNA-recognition motifs of SR proteins can regulate splicing using a noncanonical mode of RNA recognition
journal, July 2013
- Clery, A.; Sinha, R.; Anczukow, O.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 30
Structural insights into the Cyclin T1–Tat–TAR RNA transcription activation complex from EIAV
journal, November 2008
- Anand, Kanchan; Schulte, Antje; Vogel-Bachmayr, Karin
- Nature Structural & Molecular Biology, Vol. 15, Issue 12
7SK small nuclear RNA directly affects HMGA1 function in transcription regulation
journal, November 2010
- Eilebrecht, S.; Brysbaert, G.; Wegert, T.
- Nucleic Acids Research, Vol. 39, Issue 6
Recognition of GU-rich polyadenylation regulatory elements by human CstF-64 protein
journal, June 2003
- Perez Canadillas, J. M.
- The EMBO Journal, Vol. 22, Issue 11
RNA-mediated displacement of an inhibitory snRNP complex activates transcription elongation
journal, June 2010
- D'Orso, Iván; Frankel, Alan D.
- Nature Structural & Molecular Biology, Vol. 17, Issue 7
Analysis of the interaction with the hepatitis C virus mRNA reveals an alternative mode of RNA recognition by the human La protein
journal, October 2011
- Martino, Luigi; Pennell, Simon; Kelly, Geoff
- Nucleic Acids Research, Vol. 40, Issue 3
HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters
journal, March 2014
- Eilebrecht, Sebastian; Le Douce, Valentin; Riclet, Raphael
- Nucleic Acids Research, Vol. 42, Issue 8
Intracellular distribution of low molecular weight RNA species in HeLa cells
journal, November 1980
- Gurney, T.
- The Journal of Cell Biology, Vol. 87, Issue 2
A comprehensive analysis of the La-motif protein superfamily
journal, March 2009
- Bousquet-Antonelli, C.; Deragon, J. -M.
- RNA, Vol. 15, Issue 5
7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development
journal, April 2009
- Barboric, M.; Lenasi, T.; Chen, H.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 19
RNA recognition motifs: boring? Not quite
journal, June 2008
- Cléry, Antoine; Blatter, Markus; Allain, Frédéric H-T
- Current Opinion in Structural Biology, Vol. 18, Issue 3
A La protein requirement for efficient pre-tRNA folding
journal, December 2003
- Chakshusmathi, G.
- The EMBO Journal, Vol. 22, Issue 24
A novel occluded RNA recognition motif in Prp24 unwinds the U6 RNA internal stem loop
journal, June 2011
- Martin-Tumasz, Stephen; Richie, Ashley C.; Clos, Lawrence J.
- Nucleic Acids Research, Vol. 39, Issue 17
Basic Local Alignment Search Tool
journal, October 1990
- Altschul, S.
- Journal of Molecular Biology, Vol. 215, Issue 3
PPM1G Binds 7SK RNA and Hexim1 To Block P-TEFb Assembly into the 7SK snRNP and Sustain Transcription Elongation
journal, August 2015
- Gudipaty, Swapna Aravind; McNamara, Ryan P.; Morton, Emily L.
- Molecular and Cellular Biology, Vol. 35, Issue 22
Structural insight into the mechanism of stabilization of the 7SK small nuclear RNA by LARP7
journal, March 2015
- Uchikawa, Emiko; Natchiar, Kundhavai S.; Han, Xiao
- Nucleic Acids Research, Vol. 43, Issue 6
Correction: Broadening the phenotypic spectrum of pathogenic LARP7 variants: two cases with intellectual disability, variable growth retardation and distinct facial features
journal, March 2018
- Hollink, Iris H. I. M.; Alfadhel, Majid; Al-Wakeel, Anwar S.
- Journal of Human Genetics, Vol. 63, Issue 4
The La Protein
journal, June 2002
- Wolin, Sandra L.; Cedervall, Tommy
- Annual Review of Biochemistry, Vol. 71, Issue 1
New Insights into the Control of HIV-1 Transcription: When Tat Meets the 7SK snRNP and Super Elongation Complex (SEC)
journal, March 2011
- He, Nanhai; Zhou, Qiang
- Journal of Neuroimmune Pharmacology, Vol. 6, Issue 2
Recruitment of cyclin T1/P-TEFb to an HIV type 1 long terminal repeat promoter proximal RNA target is both necessary and sufficient for full activation of transcription
journal, July 1999
- Bieniasz, P. D.; Grdina, T. A.; Bogerd, H. P.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 14
Hexim1 sequesters positive transcription elongation factor b from the class II transactivator on MHC class II promoters
journal, November 2006
- Kohoutek, J.; Blazek, D.; Peterlin, B. M.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 46
The role of the C-terminal helix of U1A protein in the interaction with U1hpII RNA
journal, May 2013
- Law, M. J.; Lee, D. S.; Lee, C. S.
- Nucleic Acids Research, Vol. 41, Issue 14
7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes
journal, November 2001
- Nguyen, Van Trung; Kiss, Tamás; Michels, Annemieke A.
- Nature, Vol. 414, Issue 6861
Binding of the 7SK snRNA turns the HEXIM1 protein into a P-TEFb (CDK9/cyclin T) inhibitor
journal, June 2004
- Michels, Annemieke A.; Fraldi, Alessandro; Li, Qintong
- The EMBO Journal, Vol. 23, Issue 13
Crystal structure of a core spliceosomal protein interface
journal, January 2006
- Schellenberg, M. J.; Edwards, R. A.; Ritchie, D. B.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 5
Recognition of GU-rich polyadenylation regulatory elements by human CstF-64 protein
dataset, January 2003
- Perez-Canadillas, Jose M.; Varani, Gabriele
- Biological Magnetic Resonance Bank
A Novel RNA Binding Domain in Tetrahymena Telomerase p65 Initiates Hierarchical Assembly of Telomerase Holoenzyme
journal, February 2006
- O'Connor, C. M.; Collins, K.
- Molecular and Cellular Biology, Vol. 26, Issue 6
Protein and RNA Dynamics Play Key Roles in Determining the Specific Recognition of GU-rich Polyadenylation Regulatory Elements by Human Cstf-64 Protein
journal, April 2005
- Deka, Pritilekha; Rajan, P. K.; Perez-Canadillas, Jose Manuel
- Journal of Molecular Biology, Vol. 347, Issue 4
LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated
journal, February 2008
- Krueger, B. J.; Jeronimo, C.; Roy, B. B.
- Nucleic Acids Research, Vol. 36, Issue 7
Measurement ofJand Dipolar Couplings from Simplified Two-Dimensional NMR Spectra
journal, April 1998
- Ottiger, Marcel; Delaglio, Frank; Bax, Ad
- Journal of Magnetic Resonance, Vol. 131, Issue 2
Structural Basis for Telomerase RNA Recognition and RNP Assembly by the Holoenzyme La Family Protein p65
journal, July 2012
- Singh, Mahavir; Wang, Zhonghua; Koo, Bon-Kyung
- Molecular Cell, Vol. 47, Issue 1
Transition Step during Assembly of HIV Tat:P-TEFb Transcription Complexes and Transfer to TAR RNA
journal, September 2012
- D'Orso, I.; Jang, G. M.; Pastuszak, A. W.
- Molecular and Cellular Biology, Vol. 32, Issue 23
CTIP2 is a negative regulator of P-TEFb
journal, July 2013
- Cherrier, T.; Le Douce, V.; Eilebrecht, S.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 31
Invertebrate 7SK snRNAs
journal, January 2008
- Gruber, Andreas R.; Koper-Emde, Dorota; Marz, Manja
- Journal of Molecular Evolution, Vol. 66, Issue 2
NMR evidence for a base triple in the HIV-2 TAR C-G.C+ mutant- argininamide complex
journal, April 1998
- Brodsky, A.
- Nucleic Acids Research, Vol. 26, Issue 8
Structural analyses of the 7SK ribonucleoprotein (RNP), the most abundant human small RNP of unknown function
journal, July 1991
- Wassarman, D. A.; Steitz, J. A.
- Molecular and Cellular Biology, Vol. 11, Issue 7
Stepwise protein-mediated RNA folding directs assembly of telomerase ribonucleoprotein
journal, February 2007
- Stone, Michael D.; Mihalusova, Mariana; O’Connor, Catherine M.
- Nature, Vol. 446, Issue 7134
The La-related protein LARP7 is a component of the 7SK ribonucleoprotein and affects transcription of cellular and viral polymerase II genes
journal, May 2008
- Markert, Andreas; Grimm, Michael; Martinez, Javier
- EMBO reports, Vol. 9, Issue 6
Gbp2 interacts with THO/TREX through a novel type of RRM domain
journal, November 2015
- Martínez-Lumbreras, Santiago; Taverniti, Valerio; Zorrilla, Silvia
- Nucleic Acids Research, Vol. 44, Issue 1
Gbp2 interacts with THO/TREX through a novel type of RRM domain
journal, November 2015
- Martínez-Lumbreras, Santiago; Taverniti, Valerio; Zorrilla, Silvia
- Nucleic Acids Research, Vol. 44, Issue 1
Regulation of Polymerase II Transcription by 7SK snRNA: Two Distinct RNA Elements Direct P-TEFb and HEXIM1 Binding
journal, December 2005
- Egloff, S.; Van Herreweghe, E.; Kiss, T.
- Molecular and Cellular Biology, Vol. 26, Issue 2
The crystal structure of the Split End protein SHARP adds a new layer of complexity to proteins containing RNA recognition motifs
journal, April 2014
- Arieti, Fabiana; Gabus, Caroline; Tambalo, Margherita
- Nucleic Acids Research, Vol. 42, Issue 10
CTIP2 is a negative regulator of P-TEFb
journal, July 2013
- Cherrier, T.; Le Douce, V.; Eilebrecht, S.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 31
Distinct Conformational Transition Patterns of Noncoding 7SK snRNA and HIV TAR RNAs upon Tat Binding
journal, January 2014
- Lu, Jia; Wong, Vivian; Zhang, Yi
- Biochemistry, Vol. 53, Issue 4
Crystal structure of a core spliceosomal protein interface
journal, January 2006
- Schellenberg, M. J.; Edwards, R. A.; Ritchie, D. B.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 5
7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes
journal, November 2001
- Nguyen, Van Trung; Kiss, Tamás; Michels, Annemieke A.
- Nature, Vol. 414, Issue 6861
Structural Study of a DNA.cntdot.RNA Hybrid Duplex with a Chiral Phosphorothioate Moiety by NMR: Extraction of Distance and Torsion Angle Constraints and Imino Proton Exchange Rates
journal, September 1994
- Gonzalez, Carlos; Stec, Wojciech; Kobylanska, Anna
- Biochemistry, Vol. 33, Issue 37
Dissection of the Proposed Base Triple in Human Immunodeficiency Virus TAR RNA Indicates the Importance of the Hoogsteen Interaction †
journal, March 1997
- Tao, Jianshi; Chen, Lily; Frankel, Alan D.
- Biochemistry, Vol. 36, Issue 12
HEXIM1 targets a repeated GAUC motif in the riboregulator of transcription 7SK and promotes base pair rearrangements
journal, July 2010
- Lebars, Isabelle; Martinez-Zapien, D.; Durand, A.
- Nucleic Acids Research, Vol. 38, Issue 21
HMGA1-dependent and independent 7SK RNA gene regulatory activity
journal, January 2011
- Eilebrecht, Sebastian; Bécavin, Christophe; Léger, Hélène
- RNA Biology, Vol. 8, Issue 1
Controlling Cellular P-TEFb Activity by the HIV-1 Transcriptional Transactivator Tat
journal, October 2010
- Muniz, Lisa; Egloff, Sylvain; Ughy, Bettina
- PLoS Pathogens, Vol. 6, Issue 10
Activation and function of cyclin T–Cdk9 (positive transcription elongation factor-b) in cardiac muscle-cell hypertrophy
journal, September 2002
- Sano, Motoaki; Abdellatif, Maha; Oh, Hidemasa
- Nature Medicine, Vol. 8, Issue 11
A capping-independent function of MePCE in stabilizing 7SK snRNA and facilitating the assembly of 7SK snRNP
journal, November 2009
- Xue, Y.; Yang, Z.; Chen, R.
- Nucleic Acids Research, Vol. 38, Issue 2
An RNA Polymerase II Elongation Factor Encoded by the Human ELL Gene
journal, March 1996
- Shilatifard, A.; Lane, W. S.; Jackson, K. W.
- Science, Vol. 271, Issue 5257
Structural Basis for Telomerase RNA Recognition and RNP Assembly by the Holoenzyme La Family Protein p65
journal, July 2012
- Singh, Mahavir; Wang, Zhonghua; Koo, Bon-Kyung
- Molecular Cell, Vol. 47, Issue 1
TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts
journal, June 2009
- Shen, Yang; Delaglio, Frank; Cornilescu, Gabriel
- Journal of Biomolecular NMR, Vol. 44, Issue 4
Broadening the phenotypic spectrum of pathogenic LARP7 variants: two cases with intellectual disability, variable growth retardation and distinct facial features
journal, November 2015
- Hollink, Iris HIM; Alfadhel, Majid; Al-Wakeel, Anwar S.
- Journal of Human Genetics, Vol. 61, Issue 3
SOFAST-HMQC Experiments for Recording Two-dimensional Deteronuclear Correlation Spectra of Proteins within a Few Seconds
journal, December 2005
- Schanda, Paul; Kupče, Ēriks; Brutscher, Bernhard
- Journal of Biomolecular NMR, Vol. 33, Issue 4
Structure of the C-terminal domain of human La protein reveals a novel RNA recognition motif coupled to a helical nuclear retention element.
journal, July 2003
- Jacks, A.
- Structure., Vol. 11, Issue 7, p. 833-843
The RRM domain in GW182 proteins contributes to miRNA-mediated gene silencing
journal, March 2009
- Eulalio, Ana; Tritschler, Felix; Büttner, Regina
- Nucleic Acids Research, Vol. 37, Issue 9
CDK9/CYCLIN T1 expression during normal lymphoid differentiation and malignant transformation
journal, January 2004
- Bellan, Cristiana; De Falco, Giulia; Lazzi, Stefano
- The Journal of Pathology, Vol. 203, Issue 4
HMGA1 directly interacts with TAR to modulate basal and Tat-dependent HIV transcription
journal, March 2013
- Eilebrecht, Sebastian; Wilhelm, Emmanuelle; Benecke, Bernd-Joachim
- RNA Biology, Vol. 10, Issue 3
Binding of the 7SK snRNA turns the HEXIM1 protein into a P-TEFb (CDK9/cyclin T) inhibitor
journal, June 2004
- Michels, Annemieke A.; Fraldi, Alessandro; Li, Qintong
- The EMBO Journal, Vol. 23, Issue 13
Structural basis of G-tract recognition and encaging by hnRNP F quasi-RRMs
journal, June 2010
- Dominguez, Cyril; Fisette, Jean-François; Chabot, Benoit
- Nature Structural & Molecular Biology, Vol. 17, Issue 7
Controlling the Elongation Phase of Transcription with P-TEFb
journal, August 2006
- Peterlin, B. Matija; Price, David H.
- Molecular Cell, Vol. 23, Issue 3
Conserved and divergent features of the structure and function of La and La-related proteins (LARPs)
journal, May 2010
- Bayfield, Mark A.; Yang, Ruiqing; Maraia, Richard J.
- Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, Vol. 1799, Issue 5-6
The 7SK small nuclear RNA inhibits the CDK9/cyclin T1 kinase to control transcription
journal, November 2001
- Yang, Zhiyuan; Zhu, Qingwei; Luo, Kunxin
- Nature, Vol. 414, Issue 6861
HIV-1 Tat Assembles a Multifunctional Transcription Elongation Complex and Stably Associates with the 7SK snRNP
journal, May 2010
- Sobhian, Bijan; Laguette, Nadine; Yatim, Ahmad
- Molecular Cell, Vol. 38, Issue 3
xRRM: A new class of RRM found in the telomerase La family protein p65
journal, March 2013
- Singh, Mahavir; Choi, Charles P.; Feigon, Juli
- RNA Biology, Vol. 10, Issue 3
A La-Related Protein Modulates 7SK snRNP Integrity to Suppress P-TEFb-Dependent Transcriptional Elongation and Tumorigenesis
journal, March 2008
- He, Nanhai; Jahchan, Nadine S.; Hong, Eunmee
- Molecular Cell, Vol. 29, Issue 5
Transcription inhibition by flavopiridol: mechanism of chronic lymphocytic leukemia cell death
journal, October 2005
- Chen, Rong; Keating, Michael J.; Gandhi, Varsha
- Blood, Vol. 106, Issue 7
RNA-based affinity purification reveals 7SK RNPs with distinct composition and regulation
journal, April 2007
- Hogg, J. R.; Collins, K.
- RNA, Vol. 13, Issue 6
Evidence for a base triple in the free HIV-1 TAR RNA
journal, March 2004
- Huthoff, H.
- RNA, Vol. 10, Issue 3
The La Protein
journal, June 2002
- Wolin, Sandra L.; Cedervall, Tommy
- Annual Review of Biochemistry, Vol. 71, Issue 1
Association between the 7 S RNA and the lupus La protein varies among cell types.
journal, October 1983
- Chambers, J. C.; Kurilla, M. G.; Keene, J. D.
- Journal of Biological Chemistry, Vol. 258, Issue 19
Works referencing / citing this record:
Structural basis for recognition of human 7SK long noncoding RNA by the La-related protein Larp7
journal, June 2018
- Eichhorn, Catherine D.; Yang, Yuan; Repeta, Lucas
- Proceedings of the National Academy of Sciences, Vol. 115, Issue 28
Reconstitution of a functional 7SK snRNP
journal, April 2017
- Brogie, John E.; Price, David H.
- Nucleic Acids Research, Vol. 45, Issue 11
LARP7-like protein Pof8 regulates telomerase assembly and poly(A)+TERRA expression in fission yeast
text, January 2022
- Mennie, Amanda K.; Moser, Bettina; Nakamura, Toru
- University of Illinois at Chicago
The La and related RNA-binding proteins (LARPs): structures, functions, and evolving perspectives: The LARPs, La, and related RNA-binding proteins
journal, August 2017
- Maraia, Richard J.; Mattijssen, Sandy; Cruz-Gallardo, Isabel
- Wiley Interdisciplinary Reviews: RNA, Vol. 8, Issue 6
Structural basis of 7SK RNA 5′-γ-phosphate methylation and retention by MePCE
journal, December 2018
- Yang, Yuan; Eichhorn, Catherine D.; Wang, Yaqiang
- Nature Chemical Biology, Vol. 15, Issue 2
Swellix: a computational tool to explore RNA conformational space
journal, November 2017
- Sloat, Nathan; Liu, Jui-Wen; Schroeder, Susan J.
- BMC Bioinformatics, Vol. 18, Issue 1
Structure of S. pombe telomerase protein Pof8 C-terminal domain is an xRRM conserved among LARP7 proteins
journal, August 2019
- Basu, Ritwika; Eichhorn, Catherine D.; Cheng, Ryan
- RNA Biology