Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass

Okeke, Benedict C.

doi:10.1007/s12010-014-1121-x

Title: Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass

Abstract

Availability, cost and efficiency of microbial enzymes for lignocellulose bioconversion are central to sustainable biomass ethanol technology. Fungi enriched from decaying biomass and surface soil mixture displayed an array of strong cellulolytic and xylanolytic activities. Strains SG2 and SG4 produced a promising array of cellulolytic and xylanolytic enzymes including β-glucosidase, usually low in cultures of Trichoderma species. Nucleotide sequence analysis of internal transcribed spacer 2 (ITS2) region of rRNA gene revealed that strains SG2 and SG4 are closely related to Trichoderma inhamatum, Trichoderma piluliferum and Trichoderma aureoviride. Trichoderma sp. SG2 correspondingly displayed as much as 9.84±1.12, 48.02±2.53 and 30.10±1.11 units mL-1 of cellulase, xylanase and β-glucosidase. Ten times dilution of culture supernatant of strain SG2 revealed that activities were about 5.34, 8.45, and 2.05 orders of magnitude higher than observed in crude culture filtrate for cellulase, xylanase, and β-glucosidase respectively, indicating that more enzymes are present to contact with substrates in biomass sacharification. In parallel experiments Trichoderma species SG2 and SG4 produced more β-glucosidase than the industrial strain Trichoderma reesei RUT-C30. Results indicate that strains SG2 and SG4 have potential for low cost in-house production of primary lignocellulose-hydrolyzing enzymes for production of biomass saccharides and biofuel in the field.

Authors:

Okeke, Benedict C. ^[1]

Auburn Univ., Montgomery, AL (United States)

Publication Date:: Sun Aug 17 00:00:00 EDT 2014

Research Org.:: Auburn Univ., Montgomery, AL (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office

OSTI Identifier:: 1419492

Grant/Contract Number:: EE0003132

Resource Type:: Accepted Manuscript

Journal Name:: Applied Biochemistry and Biotechnology

Additional Journal Information:: Journal Volume: 174; Journal Issue: 4; Journal ID: ISSN 0273-2289

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; Cellulase; xylanase; β-glucosidase; β-xylosidae; biomass saccharides; lignocellulose; bioprocessing; biofuel

Citation Formats


                    Okeke, Benedict C. Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass.  United States: N. p., 2014. 
Web.  doi:10.1007/s12010-014-1121-x.

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                    Okeke, Benedict C. Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass.  United States.  https://doi.org/10.1007/s12010-014-1121-x

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                    Okeke, Benedict C. Sun .  
"Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass".  United States.  https://doi.org/10.1007/s12010-014-1121-x.  https://www.osti.gov/servlets/purl/1419492.

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@article{osti_1419492,

  title        = {Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass},

  author       = {Okeke, Benedict C.},

  abstractNote = {Availability, cost and efficiency of microbial enzymes for lignocellulose bioconversion are central to sustainable biomass ethanol technology. Fungi enriched from decaying biomass and surface soil mixture displayed an array of strong cellulolytic and xylanolytic activities. Strains SG2 and SG4 produced a promising array of cellulolytic and xylanolytic enzymes including β-glucosidase, usually low in cultures of Trichoderma species. Nucleotide sequence analysis of internal transcribed spacer 2 (ITS2) region of rRNA gene revealed that strains SG2 and SG4 are closely related to Trichoderma inhamatum, Trichoderma piluliferum and Trichoderma aureoviride. Trichoderma sp. SG2 correspondingly displayed as much as 9.84±1.12, 48.02±2.53 and 30.10±1.11 units mL-1 of cellulase, xylanase and β-glucosidase. Ten times dilution of culture supernatant of strain SG2 revealed that activities were about 5.34, 8.45, and 2.05 orders of magnitude higher than observed in crude culture filtrate for cellulase, xylanase, and β-glucosidase respectively, indicating that more enzymes are present to contact with substrates in biomass sacharification. In parallel experiments Trichoderma species SG2 and SG4 produced more β-glucosidase than the industrial strain Trichoderma reesei RUT-C30. Results indicate that strains SG2 and SG4 have potential for low cost in-house production of primary lignocellulose-hydrolyzing enzymes for production of biomass saccharides and biofuel in the field.},

  doi          = {10.1007/s12010-014-1121-x},

  journal      = {Applied Biochemistry and Biotechnology},

  number       = 4,

  volume       = 174,

  place        = {United States},

  year         = {Sun Aug 17 00:00:00 EDT 2014},

  month        = {Sun Aug 17 00:00:00 EDT 2014}

}

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Title: Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass

Abstract

Citation Formats

Transglycosylation products of cellulase system ofTrichoderma reesei journal, January 1979

Simultaneous saccharification and fermentation of lignocellulosic residues pretreated with phosphoric acid–acetone for bioethanol production journal, July 2009

Cellulases and biofuels journal, June 2009

Xylanases, xylanase families and extremophilic xylanases journal, January 2005

Lignocellulose conversion to biofuels: current challenges, global perspectives journal, June 2009

‘Cradle-to-grave’ assessment of existing lignocellulose pretreatment technologies journal, June 2009

Enhanced enzyme production from mixed cultures of Trichoderma reesei RUT-C30 and Aspergillus niger LMA grown as fed batch in a stirred tank bioreactor journal, October 2008

Bacterial and plant cellulose modification using ultrasound irradiation journal, June 2009

Biorefinery: Toward an industrial metabolism journal, June 2009

Paper mill sludge as a potential source for cellulase production by Trichoderma reesei QM 9123 and Aspergillus niger using mixed cultivation journal, January 1994

Production of cellulase/β-glucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure journal, September 2005

The potential of cellulases and cellulosomes for cellulosic waste management journal, June 2007

Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses journal, September 2010

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Cellulose: Fascinating Biopolymer and Sustainable Raw Material journal, May 2005

Surface activities, biodegradability and antimicrobial properties of n-alkyl glucosides, mannosides and galactosides journal, December 1990

Effect of Reduction in Yeast and Enzyme Concentrations in a Simultaneous- Saccharification-and-Fermentation–Based Bioethanol Process: Technical and Economic Evaluation journal, January 2005

Kinetics of solka floc cellulose hydrolysis bytrichoderma viride cellulase journal, June 1975

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Identification, purification and characterization of β-glucosidase from apple seed as a novel catalyst for synthesis of O-glucosides journal, January 2007

A novel family of hemicellulolytic α-glucuronidase journal, April 2009

What is (and is not) vital to advancing cellulosic ethanol journal, April 2007

Factors affecting cellulose hydrolysis and the potential of enzyme recycle to enhance the efficiency of an integrated wood to ethanol process journal, August 1996

Hydrolysis of lignocellulosic materials for ethanol production: a review journal, May 2002

Xylanase production by fungal mixed culture solid substrate fermentation on sugar cane bagasse journal, January 1998

An improved method to directly estimate cellulase adsorption on biomass solids journal, April 2008

Microbial xylanases and their industrial applications: a review journal, August 2001

Purification and properties of an extracellular β-xylosidase from a newly isolated Fusarium proliferatum journal, October 2003

Expression in Trichoderma reesei and characterisation of a thermostable family 3 β-glucosidase from the moderately thermophilic fungus Talaromyces emersonii journal, December 2004

Xylanase Production by Penicillium canescens 10-10c in Solid-State Fermentation journal, January 2003

Applications of enzymes in synthetic carbohydrate chemistry journal, February 1995

β-Glucosidase Production by <I>Trichoderma reesei</I> journal, January 2005

Aspergillus Enzymes Involved in Degradation of Plant Cell Wall Polysaccharides journal, December 2001

Purification and characterization of carboxymethylcellulase isolated from a marine bacterium, Bacillus subtilis subsp. subtilis A-53 journal, June 2009

Microbial xylanolytic systems journal, November 1985

Microbial β-Glucosidases: Cloning, Properties, and Applications journal, January 2002

Bacterial Reduction of Perchlorate and Nitrate in Water journal, January 1999

Properties of a xylanase from Streptomyces matensis being suitable for xylooligosaccharides production journal, June 2009

Kinetics of batch single cell protein production from rice polishings with Candida utilis in continuously aerated tank reactors journal, October 2006

Effect of xylanase supplementation of cellulase on digestion of corn stover solids prepared by leading pretreatment technologies journal, September 2009

A xylose-tolerant β-xylosidase from Paecilomyces thermophila: Characterization and its co-action with the endogenous xylanase journal, September 2008

A thermotolerant β-glucosidase isolated from an endophytic fungi, Periconia sp., with a possible use for biomass conversion to sugars journal, October 2009

Isolation of cellulose-hydrolytic bacteria and applications of the cellulolytic enzymes for cellulosic biohydrogen production journal, June 2009

Effect of additives on the digestibility of corn stover solids following pretreatment by leading technologies journal, April 2009

Enhancement of enzymatic saccharification of cellulose by cellulose dissolution pretreatments journal, May 2009

Pretreatment: the key to unlocking low-cost cellulosic ethanol journal, January 2008

Screening and identification of xylanase-producing bacteria from Thai soils journal, January 2007

The bgl1 gene of Trichoderma reesei QM 9414 encodes an extracellular, cellulose-inducible β-glucosidase involved in cellulase induction by sophorose journal, May 1995

Formation and Location of 1,4-β-Glucanases and 1,4-β-Glucosidases from Penicillium janthinellum journal, January 1981

Microbiological Procedures for Biodegradation Research book, September 2018

Canola meal as a novel substrate for β-glucosidase production by Trichoderma viride: application of the crude extract to biomass saccharification journal, June 2015

Transglycosylation products of cellulase system ofTrichoderma reesei
journal, January 1979

Simultaneous saccharification and fermentation of lignocellulosic residues pretreated with phosphoric acid–acetone for bioethanol production
journal, July 2009

Cellulases and biofuels
journal, June 2009

Xylanases, xylanase families and extremophilic xylanases
journal, January 2005

Lignocellulose conversion to biofuels: current challenges, global perspectives
journal, June 2009

‘Cradle-to-grave’ assessment of existing lignocellulose pretreatment technologies
journal, June 2009

Enhanced enzyme production from mixed cultures of Trichoderma reesei RUT-C30 and Aspergillus niger LMA grown as fed batch in a stirred tank bioreactor
journal, October 2008

Bacterial and plant cellulose modification using ultrasound irradiation
journal, June 2009

Biorefinery: Toward an industrial metabolism
journal, June 2009

Paper mill sludge as a potential source for cellulase production by Trichoderma reesei QM 9123 and Aspergillus niger using mixed cultivation
journal, January 1994

Production of cellulase/β-glucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure
journal, September 2005

The potential of cellulases and cellulosomes for cellulosic waste management
journal, June 2007

Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses
journal, September 2010

Screening for cellulose and hemicellulose degrading enzymes from the fungal genus Ulocladium
journal, June 2009

The Path Forward for Biofuels and Biomaterials
journal, January 2006

A novel cellulase free alkaliphilic xylanase from alkali tolerant Penicillium citrinum: production, purification and characterization
journal, February 2007

Hydrolysis of soybean isoflavone glycosides by a thermostable β-glucosidase from Paecilomyces thermophila
journal, August 2009

Cell-associated acid β-xylosidase production by Penicillium sclerotiorum
journal, October 2009

Effect of biomass concentration and inoculum source on the rate of anaerobic cellulose solubilization
journal, November 2009

Cellulose: Fascinating Biopolymer and Sustainable Raw Material
journal, May 2005

Surface activities, biodegradability and antimicrobial properties of n-alkyl glucosides, mannosides and galactosides
journal, December 1990

Effect of Reduction in Yeast and Enzyme Concentrations in a Simultaneous- Saccharification-and-Fermentation–Based Bioethanol Process: Technical and Economic Evaluation
journal, January 2005

Kinetics of solka floc cellulose hydrolysis bytrichoderma viride cellulase
journal, June 1975

Isolation and characterization of a β-glucosidase from Penicillium decumbens and improving hydrolysis of corncob residue by using it as cellulase supplementation
journal, May 2010

Identification, purification and characterization of β-glucosidase from apple seed as a novel catalyst for synthesis of O-glucosides
journal, January 2007

A novel family of hemicellulolytic α-glucuronidase
journal, April 2009

What is (and is not) vital to advancing cellulosic ethanol
journal, April 2007

Factors affecting cellulose hydrolysis and the potential of enzyme recycle to enhance the efficiency of an integrated wood to ethanol process
journal, August 1996

Hydrolysis of lignocellulosic materials for ethanol production: a review
journal, May 2002

Xylanase production by fungal mixed culture solid substrate fermentation on sugar cane bagasse
journal, January 1998

An improved method to directly estimate cellulase adsorption on biomass solids
journal, April 2008

Microbial xylanases and their industrial applications: a review
journal, August 2001

Purification and properties of an extracellular β-xylosidase from a newly isolated Fusarium proliferatum
journal, October 2003

Expression in Trichoderma reesei and characterisation of a thermostable family 3 β-glucosidase from the moderately thermophilic fungus Talaromyces emersonii
journal, December 2004

Xylanase Production by Penicillium canescens 10-10c in Solid-State Fermentation
journal, January 2003

Applications of enzymes in synthetic carbohydrate chemistry
journal, February 1995

β-Glucosidase Production by <I>Trichoderma reesei</I>
journal, January 2005

Aspergillus Enzymes Involved in Degradation of Plant Cell Wall Polysaccharides
journal, December 2001

Purification and characterization of carboxymethylcellulase isolated from a marine bacterium, Bacillus subtilis subsp. subtilis A-53
journal, June 2009

Microbial xylanolytic systems
journal, November 1985

Microbial β-Glucosidases: Cloning, Properties, and Applications
journal, January 2002

Bacterial Reduction of Perchlorate and Nitrate in Water
journal, January 1999

Properties of a xylanase from Streptomyces matensis being suitable for xylooligosaccharides production
journal, June 2009

Kinetics of batch single cell protein production from rice polishings with Candida utilis in continuously aerated tank reactors
journal, October 2006

Effect of xylanase supplementation of cellulase on digestion of corn stover solids prepared by leading pretreatment technologies
journal, September 2009

A xylose-tolerant β-xylosidase from Paecilomyces thermophila: Characterization and its co-action with the endogenous xylanase
journal, September 2008

A thermotolerant β-glucosidase isolated from an endophytic fungi, Periconia sp., with a possible use for biomass conversion to sugars
journal, October 2009

Isolation of cellulose-hydrolytic bacteria and applications of the cellulolytic enzymes for cellulosic biohydrogen production
journal, June 2009

Effect of additives on the digestibility of corn stover solids following pretreatment by leading technologies
journal, April 2009

Enhancement of enzymatic saccharification of cellulose by cellulose dissolution pretreatments
journal, May 2009

Pretreatment: the key to unlocking low-cost cellulosic ethanol
journal, January 2008

Screening and identification of xylanase-producing bacteria from Thai soils
journal, January 2007

The bgl1 gene of Trichoderma reesei QM 9414 encodes an extracellular, cellulose-inducible β-glucosidase involved in cellulase induction by sophorose
journal, May 1995

Formation and Location of 1,4-β-Glucanases and 1,4-β-Glucosidases from Penicillium janthinellum
journal, January 1981

Microbiological Procedures for Biodegradation Research
book, September 2018

Canola meal as a novel substrate for β-glucosidase production by Trichoderma viride: application of the crude extract to biomass saccharification
journal, June 2015