3D-Printed pHEMA Materials for Topographical and Biochemical Modulation of Dorsal Root Ganglion Cell Response
Abstract
Understanding and controlling the interactions occurring between cells and engineered materials are central challenges toward progress in the development of biomedical devices. In this work, we describe materials for direct ink writing (DIW), an extrusion-based type of 3D printing, that embed a custom synthetic protein (RGD-PDL) within the microfilaments of 3D-hydrogel scaffolds to modify these interactions and differentially direct tissue-level organization of complex cell populations in vitro. The RGD-PDL is synthesized by modifying poly-d-lysine (PDL) to varying extents with peptides containing the integrin-binding motif Arg-Gly-Asp (RGD). Compositional gradients of the RGD-PDL presented by both patterned and thin-film poly(2-hydroxyethyl) methacrylate (pHEMA) substrates allow the patterning of cell-growth compliance in a grayscale form. The surface chemistry-dependent guidance of cell growth on the RGD-PDL-modified pHEMA materials is demonstrated using a model NIH-3T3 fibroblast cell line. The formation of a more complex cellular system—organotypic primary murine dorsal root ganglion (DRG)—in culture is also achieved on these scaffolds, where distinctive forms of cell growth and migration guidance are seen depending on their RGD-PDL content and topography. In conclusion, this experimental platform for the study of physicochemical factors on the formation and the reorganization of organotypic cultures offers useful capabilities for studies in tissue engineering, regenerativemore »
- Authors:
-
[1];
[1];
[1];
[1];
- Univ. of Illinois at Urbana-Champaign, IL (United States)
- Univ. of Illinois at Urbana-Champaign, IL (United States); KTH Royal Inst. of Technology, Stockholm (Sweden)
- Publication Date:
- Research Org.:
- Univ. of Illinois at Urbana-Champaign, IL (United States)
- Sponsoring Org.:
- National Science Foundation (NSF); Army Research Office (ARO); USDOE
- OSTI Identifier:
- 1418567
- Grant/Contract Number:
- FG02-07ER46471; CBET-0939511 STC; DBI 14-50962 EAGER; IIP-1353368
- Resource Type:
- Accepted Manuscript
- Journal Name:
- ACS Applied Materials and Interfaces
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 36; Journal ID: ISSN 1944-8244
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; 4D printing; biologically compliant materials; programmable cell-scaffold interactions; 3D cell culture; functional soft materials; dorsal root ganglion (DRG); gels
Citation Formats
Badea, Adina, McCracken, Joselle M., Tillmaand, Emily G., Kandel, Mikhail E., Oraham, Aaron W., Mevis, Molly B., Rubakhin, Stanislav S., Popescu, Gabriel, Sweedler, Jonathan V., and Nuzzo, Ralph G. 3D-Printed pHEMA Materials for Topographical and Biochemical Modulation of Dorsal Root Ganglion Cell Response. United States: N. p., 2017.
Web. doi:10.1021/acsami.7b06742.
Badea, Adina, McCracken, Joselle M., Tillmaand, Emily G., Kandel, Mikhail E., Oraham, Aaron W., Mevis, Molly B., Rubakhin, Stanislav S., Popescu, Gabriel, Sweedler, Jonathan V., & Nuzzo, Ralph G. 3D-Printed pHEMA Materials for Topographical and Biochemical Modulation of Dorsal Root Ganglion Cell Response. United States. https://doi.org/10.1021/acsami.7b06742
Badea, Adina, McCracken, Joselle M., Tillmaand, Emily G., Kandel, Mikhail E., Oraham, Aaron W., Mevis, Molly B., Rubakhin, Stanislav S., Popescu, Gabriel, Sweedler, Jonathan V., and Nuzzo, Ralph G. Thu .
"3D-Printed pHEMA Materials for Topographical and Biochemical Modulation of Dorsal Root Ganglion Cell Response". United States. https://doi.org/10.1021/acsami.7b06742. https://www.osti.gov/servlets/purl/1418567.
@article{osti_1418567,
title = {3D-Printed pHEMA Materials for Topographical and Biochemical Modulation of Dorsal Root Ganglion Cell Response},
author = {Badea, Adina and McCracken, Joselle M. and Tillmaand, Emily G. and Kandel, Mikhail E. and Oraham, Aaron W. and Mevis, Molly B. and Rubakhin, Stanislav S. and Popescu, Gabriel and Sweedler, Jonathan V. and Nuzzo, Ralph G.},
abstractNote = {Understanding and controlling the interactions occurring between cells and engineered materials are central challenges toward progress in the development of biomedical devices. In this work, we describe materials for direct ink writing (DIW), an extrusion-based type of 3D printing, that embed a custom synthetic protein (RGD-PDL) within the microfilaments of 3D-hydrogel scaffolds to modify these interactions and differentially direct tissue-level organization of complex cell populations in vitro. The RGD-PDL is synthesized by modifying poly-d-lysine (PDL) to varying extents with peptides containing the integrin-binding motif Arg-Gly-Asp (RGD). Compositional gradients of the RGD-PDL presented by both patterned and thin-film poly(2-hydroxyethyl) methacrylate (pHEMA) substrates allow the patterning of cell-growth compliance in a grayscale form. The surface chemistry-dependent guidance of cell growth on the RGD-PDL-modified pHEMA materials is demonstrated using a model NIH-3T3 fibroblast cell line. The formation of a more complex cellular system—organotypic primary murine dorsal root ganglion (DRG)—in culture is also achieved on these scaffolds, where distinctive forms of cell growth and migration guidance are seen depending on their RGD-PDL content and topography. In conclusion, this experimental platform for the study of physicochemical factors on the formation and the reorganization of organotypic cultures offers useful capabilities for studies in tissue engineering, regenerative medicine, and diagnostics.},
doi = {10.1021/acsami.7b06742},
journal = {ACS Applied Materials and Interfaces},
number = 36,
volume = 9,
place = {United States},
year = {Thu Aug 31 00:00:00 EDT 2017},
month = {Thu Aug 31 00:00:00 EDT 2017}
}
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