Reversible Lifting of Surface Supported Lipid Bilayers with a Membrane-Spanning Nonionic Triblock Copolymer
Abstract
Neutron reflectometry was used to monitor structural variations in surface supported DMPC bilayers induced by the addition of Triton X-100, a surfactant commonly used to aid solubilization of membrane proteins, and the co-addition of a membrane spanning non-ionic amphiphilic triblock copolymer, (PEO117-PPO47-PEO117, Pluronic F98). Surfactant addition causes slight compression of the bilayer thickness and the creation of a distinct EO layer that increases the hydrophilic layer proximal to the supporting substrate (i.e., a water and EO gap between the lipid bilayer and quartz) to 6.8 ± 0.4 Å. Addition of the triblock copolymer into the DMPC: Triton X-100 bilayer increases the complexity (broadens) the lipid phase transition, further compresses the bilayer, and continues to expand the proximal hydrophilic layer thickness. The observed structural changes are temperature dependent with transmembrane polymer insertion achieved at 37 °C leading to a compressed membrane thickness of 39.2 ± 0.2 Å and proximal gap of 45.2 ± 0.2 Å. Temperature driven exclusion of the polymer at 15 °C causes partitioning of the polymer into the proximal space generating a large hydrogel cushion 162 ± 16 Å thick. An intermediate gap width (10 – 27 Å) is achieved at room temperature (22 – 25 °C). Themore »
- Authors:
-
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Univ. of California, Davis, CA (United States)
- Publication Date:
- Research Org.:
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC). Basic Energy Sciences (BES) (SC-22)
- OSTI Identifier:
- 1414103
- Report Number(s):
- LA-UR-17-21430
Journal ID: ISSN 1525-7797
- Grant/Contract Number:
- AC52-06NA25396
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biomacromolecules
- Additional Journal Information:
- Journal Volume: 18; Journal Issue: 4; Journal ID: ISSN 1525-7797
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; Material Science
Citation Formats
Hayden, Steven C., Junghans, Ann, Majewski, Jaroslaw, and Firestone, Millicent A. Reversible Lifting of Surface Supported Lipid Bilayers with a Membrane-Spanning Nonionic Triblock Copolymer. United States: N. p., 2017.
Web. doi:10.1021/acs.biomac.6b01461.
Hayden, Steven C., Junghans, Ann, Majewski, Jaroslaw, & Firestone, Millicent A. Reversible Lifting of Surface Supported Lipid Bilayers with a Membrane-Spanning Nonionic Triblock Copolymer. United States. https://doi.org/10.1021/acs.biomac.6b01461
Hayden, Steven C., Junghans, Ann, Majewski, Jaroslaw, and Firestone, Millicent A. Wed .
"Reversible Lifting of Surface Supported Lipid Bilayers with a Membrane-Spanning Nonionic Triblock Copolymer". United States. https://doi.org/10.1021/acs.biomac.6b01461. https://www.osti.gov/servlets/purl/1414103.
@article{osti_1414103,
title = {Reversible Lifting of Surface Supported Lipid Bilayers with a Membrane-Spanning Nonionic Triblock Copolymer},
author = {Hayden, Steven C. and Junghans, Ann and Majewski, Jaroslaw and Firestone, Millicent A.},
abstractNote = {Neutron reflectometry was used to monitor structural variations in surface supported DMPC bilayers induced by the addition of Triton X-100, a surfactant commonly used to aid solubilization of membrane proteins, and the co-addition of a membrane spanning non-ionic amphiphilic triblock copolymer, (PEO117-PPO47-PEO117, Pluronic F98). Surfactant addition causes slight compression of the bilayer thickness and the creation of a distinct EO layer that increases the hydrophilic layer proximal to the supporting substrate (i.e., a water and EO gap between the lipid bilayer and quartz) to 6.8 ± 0.4 Å. Addition of the triblock copolymer into the DMPC: Triton X-100 bilayer increases the complexity (broadens) the lipid phase transition, further compresses the bilayer, and continues to expand the proximal hydrophilic layer thickness. The observed structural changes are temperature dependent with transmembrane polymer insertion achieved at 37 °C leading to a compressed membrane thickness of 39.2 ± 0.2 Å and proximal gap of 45.2 ± 0.2 Å. Temperature driven exclusion of the polymer at 15 °C causes partitioning of the polymer into the proximal space generating a large hydrogel cushion 162 ± 16 Å thick. An intermediate gap width (10 – 27 Å) is achieved at room temperature (22 – 25 °C). The temperature-driven changes in the proximal hydrophilic gap dimensions are shown to be reversible but thermal history causes variation in magnitude. Temperature-driven changes in polymer association with a supported lipid bilayer offer a facile means to reversibly control both the membrane characteristics as well as the separation between membrane and solid substrate.},
doi = {10.1021/acs.biomac.6b01461},
journal = {Biomacromolecules},
number = 4,
volume = 18,
place = {United States},
year = {Wed Feb 22 00:00:00 EST 2017},
month = {Wed Feb 22 00:00:00 EST 2017}
}
Web of Science
Works referenced in this record:
Supported phospholipid bilayers
journal, January 1985
- Tamm, L. K.; McConnell, H. M.
- Biophysical Journal, Vol. 47, Issue 1
Solid supported lipid bilayers: From biophysical studies to sensor design
journal, November 2006
- Castellana, Edward T.; Cremer, Paul S.
- Surface Science Reports, Vol. 61, Issue 10
A review of traditional and emerging methods to characterize lipid–protein interactions in biological membranes
journal, January 2015
- Hsia, Chih-Yun; Richards, Mark J.; Daniel, Susan
- Analytical Methods, Vol. 7, Issue 17
Solid-supported lipid bilayers to drive stem cell fate and tissue architecture using periosteum derived progenitor cells
journal, March 2013
- Evans, Sarah F.; Docheva, Denitsa; Bernecker, Anja
- Biomaterials, Vol. 34, Issue 8
Supported Lipid Bilayers for the Generation of Dynamic Cell-Material Interfaces
journal, November 2015
- van Weerd, Jasper; Karperien, Marcel; Jonkheijm, Pascal
- Advanced Healthcare Materials, Vol. 4, Issue 18
Formation of Lipid Sheaths around Nanoparticle-Supported Lipid Bilayers
journal, March 2012
- Ahmed, Selver; Savarala, Sushma; Chen, Yanjing
- Small, Vol. 8, Issue 11
Lipid Nanotechnology
journal, February 2013
- Mashaghi, Samaneh; Jadidi, Tayebeh; Koenderink, Gijsje
- International Journal of Molecular Sciences, Vol. 14, Issue 2
Lipid-bilayer-assisted two-dimensional self-assembly of DNA origami nanostructures
journal, August 2015
- Suzuki, Yuki; Endo, Masayuki; Sugiyama, Hiroshi
- Nature Communications, Vol. 6, Issue 1
Model membrane systems and their applications
journal, December 2007
- Chan, Yee-Hung M.; Boxer, Steven G.
- Current Opinion in Chemical Biology, Vol. 11, Issue 6
Tethered Lipid Bilayer Membranes: Formation and Ionic Reservoir Characterization
journal, February 1998
- Raguse, Burkhard; Braach-Maksvytis, Vijoleta; Cornell, Bruce A.
- Langmuir, Vol. 14, Issue 3
Supported Membranes: Scientific and Practical Applications
journal, January 1996
- Sackmann, E.
- Science, Vol. 271, Issue 5245
Polymer-supported membranes as models of the cell surface
journal, September 2005
- Tanaka, Motomu; Sackmann, Erich
- Nature, Vol. 437, Issue 7059, p. 656-663
Formation of Solid-Supported Lipid Bilayers: An Integrated View
journal, April 2006
- Richter, Ralf P.; Bérat, Rémi; Brisson, Alain R.
- Langmuir, Vol. 22, Issue 8
Polymer Supported Lipid Bilayers
journal, January 2013
- McCabe, Ian P.; Forstner, Martin B.
- Open Journal of Biophysics, Vol. 03, Issue 01
Small-Angle X-ray Scattering Study of the Interaction of Poly(ethylene oxide)- b -Poly(propylene oxide)- b -Poly(ethylene oxide) Triblock Copolymers with Lipid Bilayers
journal, November 2003
- Firestone, Millicent A.; Wolf, Amanda C.; Seifert, Sönke
- Biomacromolecules, Vol. 4, Issue 6
Electron Density Mapping of Triblock Copolymers Associated with Model Biomembranes: Insights into Conformational States and Effect on Bilayer Structure
journal, June 2008
- Lee, Byeongdu; Firestone, Millicent A.
- Biomacromolecules, Vol. 9, Issue 6
Electron-Transfer Dynamics of Photosynthetic Reaction Centers in Thermoresponsive Soft Materials
journal, December 2005
- Laible, Philip D.; Kelley, Richard F.; Wasielewski, Michael R.
- The Journal of Physical Chemistry B, Vol. 109, Issue 49
Nanoparticle Arrays Formed by Spatial Compartmentalization in a Complex Fluid
journal, March 2001
- Firestone, Millicent A.; Williams, Dixy E.; Seifert, Sönke
- Nano Letters, Vol. 1, Issue 3
SPEAR — ToF neutron reflectometer at the Los Alamos Neutron Science Center
journal, November 2011
- Dubey, M.; Jablin, M. S.; Wang, P.
- The European Physical Journal Plus, Vol. 126, Issue 11
Co-refinement of multiple-contrast neutron/X-ray reflectivity data using MOTOFIT
journal, March 2006
- Nelson, Andrew
- Journal of Applied Crystallography, Vol. 39, Issue 2
Nanostructure of supported lipid bilayers in water
journal, September 2008
- Nickel, Bert
- Biointerphases, Vol. 3, Issue 3
Neutron Reflectivity and Atomic Force Microscopy Studies of a Lipid Bilayer in Water Adsorbed to the Surface of a Silicon Single Crystal
journal, January 1996
- Koenig, B. W.; Krueger, S.; Orts, W. J.
- Langmuir, Vol. 12, Issue 5
Phospholipid bilayer formation at a bare Si surface: a time-resolved neutron reflectivity study
journal, June 2004
- Gutberlet, T.; Steitz, R.; Fragneto, G.
- Journal of Physics: Condensed Matter, Vol. 16, Issue 26
Structure of an adsorbed dimyristoylphosphatidylcholine bilayer measured with specular reflection of neutrons
journal, February 1991
- Johnson, S. J.; Bayerl, T. M.; McDermott, D. C.
- Biophysical Journal, Vol. 59, Issue 2
Effect of Low Amounts of Cholesterol on the Swelling Behavior of Floating Bilayers
journal, September 2005
- Stidder, Barry; Fragneto, Giovanna; Roser, Stephen J.
- Langmuir, Vol. 21, Issue 20
Lipid Rearrangement in DSPC/DMPC Bilayers: A Neutron Reflectometry Study
journal, October 2012
- Gerelli, Yuri; Porcar, Lionel; Fragneto, Giovanna
- Langmuir, Vol. 28, Issue 45
Structural Studies of Polymer-Cushioned Lipid Bilayers
journal, November 1998
- Majewski, J.; Wong, J. Y.; Park, C. K.
- Biophysical Journal, Vol. 75, Issue 5
Coupling of spectrin and polylysine to phospholipid monolayers studied by specular reflection of neutrons
journal, November 1991
- Johnson, S. J.; Bayerl, T. M.; Weihan, W.
- Biophysical Journal, Vol. 60, Issue 5
MARTINI Coarse-Grained Model of Triton TX-100 in Pure DPPC Monolayer and Bilayer Interfaces
journal, April 2016
- Pizzirusso, Antonio; De Nicola, Antonio; Milano, Giuseppe
- The Journal of Physical Chemistry B, Vol. 120, Issue 16
Detergent organisation in solutions and in crystals of membrane proteins
journal, December 1994
- Timmins, Peter; Pebay-Peyroula, Eva; Welte, Wolfram
- Biophysical Chemistry, Vol. 53, Issue 1-2
Small-angle neutron scattering of silica nanoparticles templated in PEO–PPO–PEO cubic crystals
journal, February 2007
- Pozzo, Danilo C.; Walker, Lynn M.
- Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 294, Issue 1-3
Pluronic block copolymers: Evolution of drug delivery concept from inert nanocarriers to biological response modifiers
journal, September 2008
- Batrakova, Elena V.; Kabanov, Alexander V.
- Journal of Controlled Release, Vol. 130, Issue 2
Pluronic® block-copolymers in medicine: from chemical and biological versatility to rationalisation and clinical advances
journal, January 2014
- Pitto-Barry, Anaïs; Barry, Nicolas P. E.
- Polym. Chem., Vol. 5, Issue 10
Preparation of a self-supporting cell architecture mimic by water channel confined photocrosslinking within a lamellar structured hydrogel
journal, January 2011
- Grubjesic, Simonida; Lee, Byeongdu; Seifert, Sönke
- Soft Matter, Vol. 7, Issue 20
Cascade synthesis of a gold nanoparticle–network polymer composite
journal, January 2016
- Grubjesic, Simonida; Ringstrand, Bryan S.; Jungjohann, Katherine L.
- Nanoscale, Vol. 8, Issue 5
Multi-length scale evaluation of the temperature-tunable mechanical properties of a lyotropic mesophase
journal, July 2012
- Lee, Sungwon; Seifert, Sönke; Firestone, Millicent A.
- Polymer Journal, Vol. 45, Issue 2
Understanding the Interaction of Block Copolymers with DMPC Lipid Bilayer Using Coarse-Grained Molecular Dynamics Simulations
journal, November 2012
- Hezaveh, Samira; Samanta, Susruta; De Nicola, Antonio
- The Journal of Physical Chemistry B, Vol. 116, Issue 49
Nature of Interactions between PEO-PPO-PEO Triblock Copolymers and Lipid Membranes: (I) Effect of Polymer Hydrophobicity on Its Ability to Protect Liposomes from Peroxidation
journal, August 2012
- Wang, Jia-Yu; Marks, Jeremy; Lee, Ka Yee C.
- Biomacromolecules, Vol. 13, Issue 9
Nature of Interactions between PEO-PPO-PEO Triblock Copolymers and Lipid Membranes: (II) Role of Hydration Dynamics Revealed by Dynamic Nuclear Polarization
journal, August 2012
- Cheng, Chi-Yuan; Wang, Jia-Yu; Kausik, Ravinath
- Biomacromolecules, Vol. 13, Issue 9
Reflection of Neutrons from a Polymer Layer Adsorbed at the Quartz-Water Interface
journal, September 1990
- Lee, E. M.; Thomas, R. K.; Rennie, A. R.
- Europhysics Letters (EPL), Vol. 13, Issue 2
Adsorption studies of polyethers
journal, May 1999
- Shar, J. A.; Obey, T. M.; Cosgrove, T.
- Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 150, Issue 1-3
Neutron reflection from interfaces with biological and biomimetic materials
journal, May 2001
- Krueger, Susan
- Current Opinion in Colloid & Interface Science, Vol. 6, Issue 2
Neutron reflection from supported lipid membranes
journal, December 2010
- Wacklin, Hanna P.
- Current Opinion in Colloid & Interface Science, Vol. 15, Issue 6
Works referencing / citing this record:
Structure and dynamics of liposomes designed for drug delivery: coarse-grained molecular dynamics simulations to reveal the role of lipopolymer incorporation
journal, January 2020
- Lemaalem, Mohammed; Hadrioui, Nourddine; Derouiche, Abdelali
- RSC Advances, Vol. 10, Issue 7