The plasticity of cyanobacterial carbon metabolism
Abstract
This opinion article aims to raise awareness of a fundamental issue which governs sustainable production of biofuels and bio-chemicals from photosynthetic cyanobacteria. Discussed is the plasticity of carbon metabolism, by which the cyanobacterial cells flexibly distribute intracellular carbon fluxes towards target products and adapt to environmental/genetic alterations. This intrinsic feature in cyanobacterial metabolism is being understood through recent identification of new biochemical reactions and engineering on low-throughput pathways. We focus our discussion on new insights into the nature of metabolic plasticity in cyanobacteria and its impact on hydrocarbons (e.g. ethylene and isoprene) production. Here, we discuss approaches that need to be developed to rationally rewire photosynthetic carbon fluxes throughout primary metabolism. We also outline open questions about the regulatory mechanisms of the metabolic network that remain to be answered, which might shed light on photosynthetic carbon metabolism and help optimize design principles in order to improve the production of fuels and chemicals in cyanobacteria.
- Authors:
-
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office; USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1399349
- Alternate Identifier(s):
- OSTI ID: 1549234
- Report Number(s):
- NREL/JA-2700-68379
Journal ID: ISSN 1367-5931
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Current Opinion in Chemical Biology
- Additional Journal Information:
- Journal Volume: 41; Journal Issue: C; Journal ID: ISSN 1367-5931
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; biofuels; biochemicals; cyanobacteria
Citation Formats
Xiong, Wei, Cano, Melissa, Wang, Bo, Douchi, Damien, and Yu, Jianping. The plasticity of cyanobacterial carbon metabolism. United States: N. p., 2017.
Web. doi:10.1016/j.cbpa.2017.09.004.
Xiong, Wei, Cano, Melissa, Wang, Bo, Douchi, Damien, & Yu, Jianping. The plasticity of cyanobacterial carbon metabolism. United States. https://doi.org/10.1016/j.cbpa.2017.09.004
Xiong, Wei, Cano, Melissa, Wang, Bo, Douchi, Damien, and Yu, Jianping. Fri .
"The plasticity of cyanobacterial carbon metabolism". United States. https://doi.org/10.1016/j.cbpa.2017.09.004. https://www.osti.gov/servlets/purl/1399349.
@article{osti_1399349,
title = {The plasticity of cyanobacterial carbon metabolism},
author = {Xiong, Wei and Cano, Melissa and Wang, Bo and Douchi, Damien and Yu, Jianping},
abstractNote = {This opinion article aims to raise awareness of a fundamental issue which governs sustainable production of biofuels and bio-chemicals from photosynthetic cyanobacteria. Discussed is the plasticity of carbon metabolism, by which the cyanobacterial cells flexibly distribute intracellular carbon fluxes towards target products and adapt to environmental/genetic alterations. This intrinsic feature in cyanobacterial metabolism is being understood through recent identification of new biochemical reactions and engineering on low-throughput pathways. We focus our discussion on new insights into the nature of metabolic plasticity in cyanobacteria and its impact on hydrocarbons (e.g. ethylene and isoprene) production. Here, we discuss approaches that need to be developed to rationally rewire photosynthetic carbon fluxes throughout primary metabolism. We also outline open questions about the regulatory mechanisms of the metabolic network that remain to be answered, which might shed light on photosynthetic carbon metabolism and help optimize design principles in order to improve the production of fuels and chemicals in cyanobacteria.},
doi = {10.1016/j.cbpa.2017.09.004},
journal = {Current Opinion in Chemical Biology},
number = C,
volume = 41,
place = {United States},
year = {Fri Sep 29 00:00:00 EDT 2017},
month = {Fri Sep 29 00:00:00 EDT 2017}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 37 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Carbon partitioning in photosynthesis
journal, June 2013
- Melis, Anastasios
- Current Opinion in Chemical Biology, Vol. 17, Issue 3
Engineering Limonene and Bisabolene Production in Wild Type and a Glycogen-Deficient Mutant of Synechococcus sp. PCC 7002
journal, June 2014
- Davies, Fiona K.; Work, Victoria H.; Beliaev, Alexander S.
- Frontiers in Bioengineering and Biotechnology, Vol. 2
Network rigidity and metabolic engineering in metabolite overproduction
journal, June 1991
- Stephanopoulos, G.; Vallino, J.
- Science, Vol. 252, Issue 5013
Photo-catalytic conversion of carbon dioxide to organic acids by a recombinant cyanobacterium incapable of glycogen storage
journal, January 2012
- Carrieri, Damian; Paddock, Troy; Maness, Pin-Ching
- Energy & Environmental Science, Vol. 5, Issue 11, p. 9457-9461
The γ-aminobutyric acid shunt contributes to closing the tricarboxylic acid cycle in S ynechocystis sp. PCC 6803 : The γ-aminobutyric acid shunt in
journal, July 2014
- Xiong, Wei; Brune, Daniel; Vermaas, Wim F. J.
- Molecular Microbiology, Vol. 93, Issue 4
The plasticity of cyanobacterial metabolism supports direct CO2 conversion to ethylene
journal, April 2015
- Xiong, Wei; Morgan, John A.; Ungerer, Justin
- Nature Plants, Vol. 1, Issue 5
Photoheterotrophic Fluxome in Synechocystis sp. Strain PCC 6803 and Its Implications for Cyanobacterial Bioenergetics
journal, December 2014
- You, Le; He, Lian; Tang, Yinjie J.
- Journal of Bacteriology, Vol. 197, Issue 5
Phosphoketolase pathway contributes to carbon metabolism in cyanobacteria
journal, December 2015
- Xiong, Wei; Lee, Tai-Chi; Rommelfanger, Sarah
- Nature Plants, Vol. 2, Issue 1, Article No. 15187
The Entner–Doudoroff pathway is an overlooked glycolytic route in cyanobacteria and plants
journal, April 2016
- Chen, Xi; Schreiber, Karoline; Appel, Jens
- Proceedings of the National Academy of Sciences, Vol. 113, Issue 19
Glycolytic strategy as a tradeoff between energy yield and protein cost
journal, April 2013
- Flamholz, A.; Noor, E.; Bar-Even, A.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 24
Synthetic non-oxidative glycolysis enables complete carbon conservation
journal, September 2013
- Bogorad, Igor W.; Lin, Tzu-Shyang; Liao, James C.
- Nature, Vol. 502, Issue 7473
Engineering of a modular and synthetic phosphoketolase pathway for photosynthetic production of acetone from CO 2 in S ynechococcus elongatus PCC 7942 under light and aerobic condition
journal, February 2016
- Chwa, Jun‐Won; Kim, Wook Jin; Sim, Sang Jun
- Plant Biotechnology Journal, Vol. 14, Issue 8
The Metabolism of Acetate by the Blue-green Algae, Anabaena variabilis and Anacystis nidulans
journal, November 1967
- Pearce, J.; Carr, N. G.
- Journal of General Microbiology, Vol. 49, Issue 2
The Tricarboxylic Acid Cycle in Cyanobacteria
journal, December 2011
- Zhang, S.; Bryant, D. A.
- Science, Vol. 334, Issue 6062
Biochemical Validation of the Glyoxylate Cycle in the Cyanobacterium Chlorogloeopsis fritschii Strain PCC 9212
journal, April 2015
- Zhang, Shuyi; Bryant, Donald A.
- Journal of Biological Chemistry, Vol. 290, Issue 22
Role of glutamate metabolism in bacterial responses towards acid and other stresses
journal, September 2012
- Feehily, C.; Karatzas, K. A. G.
- Journal of Applied Microbiology, Vol. 114, Issue 1
Engineering the methylerythritol phosphate pathway in cyanobacteria for photosynthetic isoprene production from CO 2
journal, January 2016
- Gao, Xiang; Gao, Fang; Liu, Deng
- Energy & Environmental Science, Vol. 9, Issue 4
Techno-economic analysis of a conceptual biofuel production process from bioethylene produced by photosynthetic recombinant cyanobacteria
journal, January 2016
- Markham, Jennifer N.; Tao, Ling; Davis, Ryan
- Green Chemistry, Vol. 18, Issue 23
Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism
journal, January 2010
- Lindberg, Pia; Park, Sungsoon; Melis, Anastasios
- Metabolic Engineering, Vol. 12, Issue 1, p. 70-79
Heterologous Expression of the Mevalonic Acid Pathway in Cyanobacteria Enhances Endogenous Carbon Partitioning to Isoprene
journal, January 2014
- Bentley, Fiona K.; Zurbriggen, Andreas; Melis, Anastasios
- Molecular Plant, Vol. 7, Issue 1
Kinetic flux profiling for quantitation of cellular metabolic fluxes
journal, July 2008
- Yuan, Jie; Bennett, Bryson D.; Rabinowitz, Joshua D.
- Nature Protocols, Vol. 3, Issue 8
Transcriptome and proteome analysis of nitrogen starvation responses in Synechocystis 6803 ΔglgC, a mutant incapable of glycogen storage
journal, January 2017
- Carrieri, Damian; Lombardi, Thomas; Paddock, Troy
- Algal Research, Vol. 21
The role of dynamics in allosteric regulation
journal, December 2003
- Kern, Dorothee; Zuiderweg, Erik RP
- Current Opinion in Structural Biology, Vol. 13, Issue 6
Systematic identification of allosteric protein-metabolite interactions that control enzyme activity in vivo
journal, March 2013
- Link, Hannes; Kochanowski, Karl; Sauer, Uwe
- Nature Biotechnology, Vol. 31, Issue 4
Allosteric Inhibition of Phosphoenolpyruvate Carboxylases is Determined by a Single Amino Acid Residue in Cyanobacteria
journal, January 2017
- Takeya, Masahiro; Hirai, Masami Yokota; Osanai, Takashi
- Scientific Reports, Vol. 7, Issue 1
The Unique Cyanobacterial Protein OpcA Is an Allosteric Effector of Glucose-6-phosphate Dehydrogenase in Nostoc punctiforme ATCC 29133
journal, January 2001
- Hagen, Kari D.; Meeks, John C.
- Journal of Biological Chemistry, Vol. 276, Issue 15
Structural and biochemical characterization of fructose-1,6/sedoheptulose-1,7-bisphosphatase from the cyanobacterium Synechocystis strain 6803
journal, December 2013
- Feng, Lingling; Sun, Yao; Deng, Hui
- FEBS Journal, Vol. 281, Issue 3
The diversity of cyanobacterial metabolism: genome analysis of multiple phototrophic microorganisms
journal, January 2012
- Beck, Christian; Knoop, Henning; Axmann, Ilka M.
- BMC Genomics, Vol. 13, Issue 1
Different strategies of metabolic regulation in cyanobacteria: from transcriptional to biochemical control
journal, September 2016
- Jablonsky, Jiri; Papacek, Stepan; Hagemann, Martin
- Scientific Reports, Vol. 6, Issue 1
Rerouting Carbon Flux To Enhance Photosynthetic Productivity
journal, February 2012
- Ducat, Daniel C.; Avelar-Rivas, J. Abraham; Way, Jeffrey C.
- Applied and Environmental Microbiology, Vol. 78, Issue 8
Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria
journal, January 2012
- Gao, Zhengxu; Zhao, Hui; Li, Zhimin
- Energy Environ. Sci., Vol. 5, Issue 12
Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde
journal, November 2009
- Atsumi, Shota; Higashide, Wendy; Liao, James C.
- Nature Biotechnology, Vol. 27, Issue 12, p. 1177-1180
Engineering cyanobacteria for photosynthetic production of 3-hydroxybutyrate directly from CO2
journal, March 2013
- Wang, Bo; Pugh, Shawn; Nielsen, David R.
- Metabolic Engineering, Vol. 16
Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light
journal, November 2013
- Kusakabe, Tamami; Tatsuke, Tsuneyuki; Tsuruno, Keigo
- Metabolic Engineering, Vol. 20
Works referencing / citing this record:
New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering
journal, February 2019
- Santos-Merino, María; Singh, Amit K.; Ducat, Daniel C.
- Frontiers in Bioengineering and Biotechnology, Vol. 7
Membrane-Inlet Mass Spectrometry Enables a Quantitative Understanding of Inorganic Carbon Uptake Flux and Carbon Concentrating Mechanisms in Metabolically Engineered Cyanobacteria
journal, June 2019
- Douchi, Damien; Liang, Feiyan; Cano, Melissa
- Frontiers in Microbiology, Vol. 10
Remodeling of the Photosynthetic Chain Promotes Direct CO 2 Conversion into Valuable Aromatic Compounds
journal, October 2018
- Ni, Jun; Liu, Hong‐Yu; Tao, Fei
- Angewandte Chemie, Vol. 130, Issue 49
Biotechnology of cyanobacterial isoprene production
journal, May 2018
- Chaves, Julie E.; Melis, Anastasios
- Applied Microbiology and Biotechnology, Vol. 102, Issue 15
Unlocking the photobiological conversion of CO 2 to ( R )-3-hydroxybutyrate in cyanobacteria
journal, January 2018
- Wang, Bo; Xiong, Wei; Yu, Jianping
- Green Chemistry, Vol. 20, Issue 16
Terpenoid Metabolic Engineering in Photosynthetic Microorganisms
journal, October 2018
- Vavitsas, Konstantinos; Fabris, Michele; Vickers, Claudia
- Genes, Vol. 9, Issue 11
Recent advances in synthetic biology of cyanobacteria
journal, May 2018
- Sengupta, Annesha; Pakrasi, Himadri B.; Wangikar, Pramod P.
- Applied Microbiology and Biotechnology, Vol. 102, Issue 13
Remodeling of the Photosynthetic Chain Promotes Direct CO 2 Conversion into Valuable Aromatic Compounds
journal, December 2018
- Ni, Jun; Liu, Hong‐Yu; Tao, Fei
- Angewandte Chemie International Edition, Vol. 57, Issue 49
New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering
journal, February 2019
- Santos-Merino, María; Singh, Amit K.; Ducat, Daniel C.
- Frontiers in Bioengineering and Biotechnology, Vol. 7
Membrane-Inlet Mass Spectrometry Enables a Quantitative Understanding of Inorganic Carbon Uptake Flux and Carbon Concentrating Mechanisms in Metabolically Engineered Cyanobacteria
journal, June 2019
- Douchi, Damien; Liang, Feiyan; Cano, Melissa
- Frontiers in Microbiology, Vol. 10
Enhanced Production of D-Lactate in Cyanobacteria by Re-Routing Photosynthetic Cyclic and Pseudo-Cyclic Electron Flow
journal, January 2020
- Selão, Tiago Toscano; Jebarani, Jasmin; Ismail, Nurul Aina
- Frontiers in Plant Science, Vol. 10
Terpenoid Metabolic Engineering in Photosynthetic Microorganisms
journal, October 2018
- Vavitsas, Konstantinos; Fabris, Michele; Vickers, Claudia
- Genes, Vol. 9, Issue 11